Metabolomics Analysis of Phase Shift and Intercellular Entrainment of Central Circadian Pacemaker Cells

• Project/Area Number: 20310119
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 基礎ゲノム科学
• Research Institution: Kanazawa University (2009-2011); Mitsubishi Kagaku Institute of Life Sciences (2008)
• Principal Investigator: TEI Hajime 金沢大学, 自然システム学系, 教授 (00242115)
• Co-Investigator(Kenkyū-buntansha): 曽我 朋義 慶應義塾大学, 環境情報学部, 教授 (60338217)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
• Keywords: 概日リズム / 視交叉上核 / 時計遺伝子 / メタボローム / データベース / CE-MS / メクボローム

Research Abstract

This study is focused on the phase shift and inter-cellular entrainment of the cellular circadian rhythms. To clarify the molecule(s) these phenomena, I have constructed a database assembling the results of metabolomic and proteomic analyses. Metabolites involved in the determination of cellular energy states, lipid metabolism, and amino acid biosynthesis worked for the cellular phase shift ; and those involved in xenobiotics and nucleic acid metabolism functioned for cellular entrainment

Research Products

• [Journal Article] Positive Autoregulation Delays the Expression Phase of Mammalian Clock Gene Per2 (2011)
  • Author(s): Ogawa, Y., Koike, N., Kurosawa, G., Soga, T., Tomita, M., Tei, H.
  • Journal Title: PLoS One Volume: 6
  • Peer Reviewed
• [Journal Article] Positive Autoregulation Delays the Expression Phase of Mammalian Clock Gene Per2 (2011)
  • Author(s): Ogawa Y, Koike N, Kurosawa G, Soga T, Tomita M, Tei H
  • Journal Title: PLoS One Volume: 6
  • Peer Reviewed
• [Journal Article] Positive Autoregulation Delays the Expression Phase of Mammalian Clock Gene Per2. (2011)
  • Author(s): Ogawa Y, Koike N, Kurosawa G, Soga T, Tomita M, Tei H.
  • Journal Title: PLoS One. 6
• [Journal Article] Positive Autoregulation Delays the Expression Phase of Mammalian Clock Gene Per2. (2011)
  • Author(s): Ogawa Y, Koike N, Kurosawa G, Soga T, Tomita M, Tei H
  • Journal Title: PLoS One Volume: 6
  • Peer Reviewed
• [Journal Article] The uterus sustains stable biological clock during pregnancy (2010)
  • Author(s): Akiyama, S., Ohta, H., Watanabe, S., Moriya, T., Hariu, A., Nakahata, N., Chisaka, H., Matsuda, T., Kimura, Y., Tsuchiya, S., Tei, H., Okamura, K., Yaegashi, N.
  • Journal Title: Tohoku J. Exp. Med Volume: 221 Pages: 287-298
  • NAID: 10027085356
  • Peer Reviewed
• [Journal Article] The uterus sustains stable biological clock during pregnancy. (2010)
  • Author(s): Akiyama, S., Ohta, H., Watanabe, S., Moriya, T., Hariu, A., Nakahata, N.,Chisaka, H., Matsuda, T., Kimura, Y.,Tsuchiya, S., Tei, H., Okamura, K.,Yaegashi, N.
  • Journal Title: Tohoku J. Exp. Med. 221 Pages: 287-298
  • NAID: 10027085356
• [Journal Article] The uterus sustains stable biological clock during pregnancy. (2010)
  • Author(s): Akivama S, Ohta H, Watanabe S, Mori ya T, Hariu A, Nakahata N, Chisaka H, Matsuda T, Kimura Y.Tsuchlva S, Tei_H.Okamura K, Yaedashi N.
  • Journal Title: Tohoku J Exp Med. Volume: 221 Pages: 287-298
  • NAID: 10027085356
  • Peer Reviewed
• [Journal Article] Period-phase map : two-dimensional selection of circadian rhythm-related genes (2009)
  • Author(s): Morioka, R., Arita, M., Sakamoto, K., Kawaguchi, S., Tei, H., Horimoto, K.
  • Journal Title: IET Syst Biol Volume: 3 Pages: 487-495
  • Peer Reviewed
• [Journal Article] Genetic and Molecular Analysis of Wild-Derived Arrhythmic Mice (2009)
  • Author(s): Watanabe, T., Suzuki, T., Ishikawa, A., Yokota, Y., Ueda, H. R., Yamada, R. G., Tei, H., Imai, S., Tomida, S., Kobayashi, J., Naito, E., Yasuo, S., Nakao, N., Namikawa, T., Yoshimura, T., Ebihara, S.
  • Journal Title: PLoS ONE Volume: 4
  • Peer Reviewed
• [Journal Article] Ontogeny of circadian organization in the rat (2009)
  • Author(s): Yamazaki, S., Yoshikawa, T., Biscoe, E. W., Numano, R., Gallaspy, L. M., Soulsby, S., Papadimas, E., Pezuk, P., Doyle, S. E., Tei, H., Sakaki, Y., Block, G. D., Menaker, M.
  • Journal Title: J Biol Rhythms Volume: 24 Pages: 55-63
  • Peer Reviewed
• [Journal Article] Period-phase map : two-dimensional selection of circadian rhythm-related genes. (2009)
  • Author(s): Morioka, R., Arita, M., Sakamoto, K., Kawaguchi, S., Tei, H., Horimoto, K.
  • Journal Title: IET Syst Biol. 3 Pages: 487-495
• [Journal Article] Genetic and Molecular Analysis of Wild-Derived Arrhythmic Mice. (2009)
  • Author(s): Watanabe, T., Suzuki, T., Ishikawa, A., Yokota, Y., Ueda, H.R., Yamada, R.G., Tei, H., Imai, S., Tomida, S., Kobayashi, J., Naito, E., Yasuo, S., Nakao, N., Namikawa, T., Yoshimura, T., Ebihara, S.
  • Journal Title: PLoS ONE. 4
• [Journal Article] Ontogeny of circadian organization in the rat. (2009)
  • Author(s): Yamazaki, S., Yoshikawa, T., Biscoe, E.W., Numano, R., Gallaspy, L.M., Soulsby, S., Papadimas, E., Pezuk, P., Doyle, S.E., Tei, H., Sakaki, Y., Block, G.D., Menaker, M.
  • Journal Title: J Biol Rhythms. 24 Pages: 55-63
• [Journal Article] Period-phase map: two-dimensional selection of circadian rhythm-related genes (2009)
  • Author(s): Morioka R, Arita M, Sakamoto K, Kawaguchi S, Tei H, Horimoto K.
  • Journal Title: IET Syst.Biol. 3 Pages: 487-495
  • Peer Reviewed
• [Journal Article] Genetic and molecular analysis of wild-derived arrhythmic mice. (2009)
  • Author(s): Watanabe T, Suzuki T, Ishikawa A, Yokota Y, Ueda HR, Yamada RG, Tei H, Imai S, Tomida S, Kobayashi J, Naito E, Yasuo S, Nakao N, Namikawa T, Yoshimura T, Ebihara S.
  • Journal Title: PLoS One 4
  • Peer Reviewed
• [Journal Article] Ontogeny of circadian organization in the rat. (2009)
  • Author(s): Yamazaki S, Yoshikawa T, Biscoe EW, Numano R, Gallaspy LM, Soulsby S, Papadimas E, Pezuk P, Doyle SE, Tei H, Sakaki Y, Block GD, Menaker M.
  • Journal Title: J.Biol.Rhythms 24 Pages: 55-63
  • Peer Reviewed
• [Journal Article] Ontogeny of circadian organization in the rat (2009)
  • Author(s): Yamazaki S, Yoshikawa T, Biscoe EW, Numano R, Gallaspy LM, Soulsby S, Papadimas E, Pezuk P, Doyle SE, Tei H, Sakaki Y, Block GD, Menaker M.
  • Journal Title: J Biol Rhythms 24 Pages: 55-63
  • Peer Reviewed
• [Journal Article] Genetic and Molecular Analysis of Wild-Derived Arrhythmic Mice (2009)
  • Author(s): Watanabe T., Suzuki T., Ishikawa A., Yokota Y., Ueda HR., Yamada RG., Tei H., Imai S., Tomida S., Kobayashi J., Naito E., Yasuo S., Nakao N., Namikawa T., Yoshimura T., Ebihara S.
  • Journal Title: PLoS ONE 4
  • Peer Reviewed
• [Journal Article] Quantitative Metabolome Profiling of Colon and Stomach Cancer Microenvironment by Capillary Electrophoresis Time-of-flight Mass Spectrometry (2009)
  • Author(s): Hirayama, A., Kami, K., Sugimoto, M., Sugawara, M., Toki, N., Onozuka, H., Kinoshita, T., Saito, N., Ochiai, A., Tomita, M., Esumi, H., Soga, T.
  • Journal Title: Cancer Res. 69 Pages: 4918-4925
  • Peer Reviewed
• [Journal Article] Cystathionine β-synthase as a Carbon Monoxide-sensitive Regulator of Bile Excretion. (2009)
  • Author(s): Shintani, T., Iwabuchi, T., Soga, T., Kato, Y., Yamamoto, T., Takano, N., Hishiki, T., Ueno, Y., Ikeda, S., Sakurakawa, T., Ishikawa, K., Goda, N., Yitagawa, Y., Kajiyama, M., Matsumoto, K., Suematsu, M.
  • Journal Title: Hepatology 49 Pages: 141-150
  • Peer Reviewed
• [Journal Article] Degradation of ppGpp by nudix pyrophosphatase modulates the transition of growth phase in the bacterium thermos thermophilus (2009)
  • Author(s): Ooga, T., Ohashi, Y., Kuramitsu, S., Koyama, Y., Tomita, M., Soga, T., Masui, R.
  • Journal Title: J. Biol. Chem.
  • Peer Reviewed
• [Journal Article] Metabolite profiling reveals YihU as a novel hydroxybutyrate dehydrogenase for alternative succinic semialdehyde metabolism in Escherichia coli. (2009)
  • Author(s): Saito, N., Robert, M., Kochi, H., Matsuo, G., Kakazu, Y., Soga, T., Tomita, M.
  • Journal Title: J. Biol. Chem.
  • Peer Reviewed
• [Journal Article] Maternal feeding controls fetal biological clock (2008)
  • Author(s): Ohta, H., Xu, S., Moriya, T., Iigo, M., Watanabe, T., Nakahata, N., Chisaka, H., Hanita, T., Matsuda, T., Ohura, T., Kimura, Y., Yaegashi, N., Tsuchiya, S., Tei, H., Okamura, K.
  • Journal Title: PLoS ONE Volume: 3
  • Peer Reviewed
• [Journal Article] Maternal feeding controls fetal biological clock. (2008)
  • Author(s): Ohta, H., Xu, S., Moriya, T., Iigo, M., Watanabe, T., Nakahata, N., Chisaka, H., Hanita, T., Matsuda, T., Ohura, T., Kimura, Y., Yaegashi, N., Tsuchiya, S., Tei, H., Okamura, K.
  • Journal Title: PLoS ONE. 3
• [Journal Article] Circadian mPer1 gene expression in mesencephalic trigeminal nucleus cultures. (2008)
  • Author(s): Hiler, DJ., Bhattacherjee, A., Yamazaki, S., Tei, H, Geusz, ME.
  • Journal Title: Brain Res. 1214 Pages: 84-93
• [Journal Article] Reorganization of the suprachiasmatic nucleus coding for day length. (2008)
  • Author(s): Naito, E., Watanabe, T., Tei, H., Yoshimura, T., Ebihara, S.
  • Journal Title: J. Biol. Rhythms. 23 Pages: 140-149
• [Journal Article] Reorganization of the suprachiasmatic nucleus coding for day length (2008)
  • Author(s): Naito E., Watanabe T., Tei, H., Yoshimura T., Ebihara, S.
  • Journal Title: J. Biol. Rhythms. 23 Pages: 140-149
  • Peer Reviewed
• [Journal Article] Circadian mPer1 gene expression in mesencephalic trigeminal nucleus cultures (2008)
  • Author(s): Hiler, DJ., Bhattacherjee, A., Yamazaki, S., Tei, H, Geusz, ME.
  • Journal Title: Brain Res. 1214 Pages: 84-93
  • Peer Reviewed
• [Journal Article] Maternal feeding controls fetal biological clock (2008)
  • Author(s): Ohta, H., Xu, S., Moriya, T., Iigo, M., Watanabe, T., Nakahata, N., Chisaka, H., Hanita, T., Matsuda, T., Ohura, T., Kimura, Y., Yaegashi, N., Tsuchiya, S., Tei. H., Okamura, K.
  • Journal Title: PLoS ONE 3
  • Peer Reviewed