A novel PVDF membrane for on-membrane identification of gel-resolved proteins by mass spectrometry

• Project/Area Number: 20310121
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied genomics
• Research Institution: Yokohama City University
• Principal Investigator: HIRANO Hisashi Yokohama City University, 生命ナノシステム科学研究科, 教授 (00275075)
• Co-Investigator(Kenkyū-buntansha): KAWASAKI HIROSHI 横浜市立大学, 生命ナノシステム科学研究科, 准教授 (70169704) ; ARAKAWA NORIAKI 横浜市立大学, 生命ナノシステム科学研究科, 助教 (60398394)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2008: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
• Keywords: タンパク質 / 翻訳後修飾 / プロテインチップ / 質量分析装置 / 癌

Research Abstract

In the 1990s, techniques were developed to transfer proteins from gels onto PVDF membranes, and to identify the proteins on the membranes by MALDI-MS. However, the low ionization efficiency of peptides and proteins immobilized on the membranes often renders these techniques useless. Therefore, it is essential to develop membranes on which peptides and proteins can be efficiently ionized for MS analysis. In this study, we found that 0.1-μm pores PVDF membranes can be used for highly efficient and effective electroblotting and MALDI-MS analysis. This membrane showed high ion yield in MALDI-MS analysis of peptides compared to conventional PVDF membranes. In all, 38 yeast proteins with various physicochemical characteristics were separated by 2-DE, immobilized onto the 0.1-μm pore PVDF membranes, and successfully identified by MALDI-MS/MS.

Research Products

• [Journal Article] Proteomic identification of differentially expressed genes in neural stem cells andneurons differentiated from embryonic stem cells of cynomolgus monkey (Macaca fascicularis) in vitro. (2011)
  • Author(s): Akama K, Horikoshi T, Nakayama T, Otsu M, Imaizumi N, Nakamura M, Toda T, Inuma I, Hirano H, Kondo K, Suzuki S, Inoue N.
  • Journal Title: Biophys.Biochim.Acta 1814 Pages: 265-276
  • Peer Reviewed
• [Journal Article] Nα-Acetylation of yeast ribosomal proteins : Identification by 2D-DIGE MS/MS and analysis of the effect on proteins synthesis. (2011)
  • Author(s): Kamita M, Kimura Y, Ino Y, Kamp RM, Polavoda B, Sherman F, Hirano H.
  • Journal Title: J Proteomics 74 Pages: 431-441
  • Peer Reviewed
• [Journal Article] Proteomic identification of differentially expressed genes in neural stemcells andneurons differentiated f rom embryonic stem cells of cynomolgus monkey (Macaca fascicularis) in vitro. (2011)
  • Author(s): Akama K, Horikoshi T, Nakayama T, Otsu M, Imaizumi N, Nakamura M, Toda T, Inuma I, Hirano H, Kondo K, Suzuki S, Inoue N
  • Journal Title: Biophys.Biochim.Acta Volume: 1814 Pages: 265-276
  • Peer Reviewed
• [Journal Article] N^α-Acetylation of yeast ribosomal proteins : Identification by 2D-DIGE MS/MS and analysis of the effect on proteins synthesis. (2011)
  • Author(s): Kamita M, Kimura Y, Ino Y, Kamp R M, Polavoda B, Sherman F, Hirano H
  • Journal Title: Journal of Priteomics Volume: 74 Pages: 431-441
  • Peer Reviewed
• [Journal Article] Co- and post-translational modifications of the 26S proteasome. (2010)
  • Author(s): Kikuchi J, Iwafune Y, Akiyama T, Okayama A, Nakamura H, Arakawa N, Kimura Y, Hirano H.
  • Journal Title: Proteomics 10 Pages: 2769-2779
  • Peer Reviewed
• [Journal Article] Characterization of multiple alternative forms of heterogeneous nuclear ribonucleoprotein K by phosphate-affinity electrophoresis. (2010)
  • Author(s): Kimura Y, Nagata K, Suzuki N, Yokoyama R, Yamanaka Y, Kitamura H, Hirano H, Ohara O.
  • Journal Title: Proteomics 10 Pages: 3884-3895
  • Peer Reviewed
• [Journal Article] Characterization of multiple alternative forms of heterogeneous nuclear ribonucleoprotein K by phosphate-affinity electrophoresis. (2010)
  • Author(s): Kimura Y, Nagata K, Suzuki N, Yokoyama R, Yamanaka Y, Kitamura H, Hirano H, Ohara O
  • Journal Title: Proteomics Volume: 10 Pages: 3884-3895
  • Peer Reviewed
• [Journal Article] Co- and post-translational modifications of the 26S proteasome in yeast (2010)
  • Author(s): Kikuchi J, Iwafune Y, Akiyama T, Okayama A, Nakamura H, Arakawa N, Kimura Y, Hirano H
  • Journal Title: Proteomics (in press)
  • Peer Reviewed
• [Journal Article] Characterization of receptor proteins using affinity cross-linking with biotinylated ligands (2010)
  • Author(s): Shinya T, Osada T, Desaki Y, Hatamoto M, Yamanaka Y, Hirano H, Takai R, Che F-S, Kaku H, Shibuya N
  • Journal Title: Plant Cell Physiol 51 Pages: 262-270
  • NAID: 10027346826
  • Peer Reviewed
• [Journal Article] Proteomic analysis of membrane proteins expressed specifically in pluripoten stem cells (2009)
  • Author(s): Itoh A, Kurisaki A, Yamanaka Y, Hirano H, Fukuda H, Sugino H, Asashima M.
  • Journal Title: Proteomics 9 Pages: 126-137
  • Peer Reviewed
• [Journal Article] Transgenic peas expressing an a-amylase inhibitor gene from beans show altered expression and modification of endogenous proteins (2009)
  • Author(s): Islam N, Campbell PM, Higgins TJV, Hirano H, Arkurst RJ
  • Journal Title: Electrophoresis 30 Pages: 1863-1868
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 平野久
  • Journal Title: タンパク質のアミノ酸配列と翻訳後修飾の分析, やさしい原理からはいるタンパク質化学実験法, タンパク質をみる-構造と挙動-(化学同人, 京都) Pages: 1-32
• [Journal Article] (2009)
  • Author(s): Kamp K, Hirano H
  • Journal Title: N-Terminal sequencing of N-terminally modified proteins. In : The Protein Protocols Handbook(Humana Press, New York) Pages: 1063-1080
• [Journal Article] (2009)
  • Author(s): 木村弥生, 平野久
  • Journal Title: iTRAQ試薬を用いた疾患バイオマーカー探索, 明日を拓く新次元プロテオミクス(秀潤社, 東京, 2009) Pages: 116-121
• [Journal Article] Proteomic analysis of membrane proteins expressed specifically in pluripoten stem cells (2009)
  • Author(s): Itoh, A., Kurisaki, A., Yamanaka, Y., Hirano, H., Fukuda, H., Sugino, H., Asashima, M
  • Journal Title: Proteomics 9 Pages: 126-137
  • Peer Reviewed
• [Journal Article] Mutagenesis of longer inserts by the ligation of two PCR fragments amplified with a mutation primer (2009)
  • Author(s): Kato, Y., Arakawa, N., Mashuishi, Y., Kawasaki, H., Hirano H.
  • Journal Title: J. Biosci. Bioeng. 107 Pages: 95-97
  • NAID: 110007042029
  • Peer Reviewed
• [Journal Article] A signaling polypeptide derived from an innate immune adaptor molecule can be harnessed as a new class of vaccine adjuvant. (2009)
  • Author(s): Kobiyama, K, . Takeshita, F., Ishii, K. J., Koyama, S., Aoshi, T., Akira, S., Sakaue-Sawano, A., Miyawaki, A., Yamanaka, Y., Hirano, H., Suzuki, K., Okuda, K.
  • Journal Title: J Immunol. 182 Pages: 1593-1601
  • Peer Reviewed
• [Journal Article] Wild-type p53 enhances Annexin IV gene expression in ovarian clear cell adenocarcinoma.
  • Author(s): Masuishi Y, Arakawa N, Hirano H.
  • Journal Title: FEBS J in press
  • Peer Reviewed
• [Journal Article] Wild-type p53 enhances Annexin IV gene expression in ovarian clear cell adenocarcinoma.
  • Author(s): Masuishi Y, Arakawa N, Hirano H
  • Journal Title: FEBS J. Volume: (in press)
  • Peer Reviewed
• [Presentation] 比較リン酸化プロテオーム解析による腫瘍組織型特異的なリン酸化修飾異常蛋白質の探索 (2010)
  • Author(s): 木村鮎子, 野村文子, 永田佳代子, 荒川憲昭, 平野久
  • Organizer: 第33回日本分子生物学会年会,第83回日本生化学会大会合同大会
  • Place of Presentation: 兵庫,神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] Proteome analysis for the discovery of biomarkers and therapeutic targets. (2010)
  • Author(s): Hirano H, Arakawa N, Masuishi Y, Morita E., Miyagi E, Hirahara F.
  • Organizer: 5th AOHUPO Congress, 14th ADNAT Convention & 1st PSI Conference
  • Place of Presentation: ハイデラバード
  • Year and Date: 2010-02-24
• [Presentation] Proteome analysis for the discovery of biomarkers and therapeutic targets (2010)
  • Author(s): Hirano H, Arakawa N, Masuishi Y, Morita E, Miyagi E, Hirahara F
  • Organizer: 5th AOHUPO Congress, 14th ADNAT Convention & 1st PSI Conference
  • Place of Presentation: Hydrabad, India
  • Year and Date: 2010-02-24
• [Presentation] A novel PVDF membrane for on-membrane identification of gel-resolved proteins by MALDI MS (2010)
  • Author(s): Ino Y, Okayama A, Arakawa N, Hirano H.
  • Organizer: The 17th Meeting of Methods in Protein Structure Analysis
  • Place of Presentation: ウプサラ
• [Presentation] 卵巣明細胞腺癌のセクレトーム解析 (2010)
  • Author(s): 荒川憲昭, 森田絵理菜, 宮城悦子, 平原史樹, 平野久
  • Organizer: 日本プロテオーム機構第8回大会・第6回日本臨床プロテオーム研究会連合大会
  • Place of Presentation: 千葉,東京ベイホテル
• [Presentation] ヘテロ核リボヌクレオタンパク質のモディフィコミクス (2010)
  • Author(s): 木村弥生, 永田佳代子, 北村浩, 平野久, 小原牧
  • Organizer: 日本プロテオーム機構第8回大会・第6回日本臨床プロテオーム研究会連合大会
  • Place of Presentation: 千葉,東京ベイホテル
• [Presentation] A novel PVDF membrane for on-membrane identification of gel-resolved proteins by MALDI MS (2010)
  • Author(s): Ino Y, Okayama A, Arakawa N, Hirano H
  • Organizer: The 17th Meeting of Methods in Protein Structure Analysis
  • Place of Presentation: Sweden, Uppsala
• [Presentation] ヒト26Sプロテアソームのリン酸化による機能調節 (2010)
  • Author(s): 木村弥生, 永田佳代子, 菅原英俊, 井野洋子, 野村文子, 小原牧, 鵜殿平一郎, 平野久
  • Organizer: 第61回日本電気泳動学会
  • Place of Presentation: 北海道,北海道大学
• [Presentation] Secretome analysis of cell lines derived from ovarian clear cell adenocarcinoma, a highly malignant type of ovarian cancer. (2010)
  • Author(s): Arakawa N, Morita E, Nomura A, Miyagi E, Hirahara F, Kawasaki H, Hirano H
  • Organizer: 9th Human Proteome Organisation World Congress
  • Place of Presentation: Australia, Sydney
• [Presentation] 診断マーカーのプロテオミクス,探索,バリデーション,利用の方法 (2009)
  • Author(s): 平野久
  • Organizer: 第60回日本電気泳動学会総会
  • Place of Presentation: 松本
  • Year and Date: 2009-09-20
• [Presentation] 診断マーカーのプロテオミクス, 探索, バリデーション, 利用の方法 (2009)
  • Author(s): 平野久
  • Organizer: 第60回日本電気泳動学会総会
  • Place of Presentation: 松本
  • Year and Date: 2009-09-20
• [Presentation] Proteomic analysis of co- and post-translational modifications in the yeast 26S proteasome. (2009)
  • Author(s): 平野久
  • Organizer: 11th International Congress on Amino Acids, Peptides and Proteins
  • Place of Presentation: ウイーン
  • Year and Date: 2009-08-06
• [Presentation] Proteomic analysis of co- and post-translational modifications in the yeast 26S proteasome (2009)
  • Author(s): 平野久
  • Organizer: 11th International Congress on Amino Acids, Peptides and Proteins
  • Place of Presentation: Vienna
  • Year and Date: 2009-08-06
• [Presentation] バイオマーカーおよび創薬ターゲット探索のプロテオーム研究における質量分析 (2009)
  • Author(s): 平野久
  • Organizer: 第61回日本細胞生物学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-04
• [Presentation] バイオマーカー及び創薬ターゲット探索のプロテオーム研究における質量分析 (2009)
  • Author(s): 平野久
  • Organizer: 第61回日本細胞生物学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-04
• [Presentation] 蛋白質複合体の翻訳後修飾のプロテオーム解析 (2009)
  • Author(s): 平野久
  • Organizer: 第73日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2009-05-23
• [Presentation] 蛋白質複合体の翻訳後修飾のプロテオーム解祈 (2009)
  • Author(s): 平野久
  • Organizer: 第73日本生化学会中部支部例会・シンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2009-05-23
• [Presentation] プロテオーム解析による食品安全性評価 (2009)
  • Author(s): 平野 久, 倉田 洋一, Islam, N., 森司
  • Organizer: 日本農芸化学会講演会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-28
• [Presentation] 卵巣明細胞腺癌特異的なアネキシンIV遺伝子の発現に関わる転写製御因子の同定 (2008)
  • Author(s): 増石有佑, 荒川憲昭, 川崎博史, 宮城悦子, 平原史樹, 平野久
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] 卵巣明細胞腺癌におけるリン脂質結合タンパク質アネキシンIVの性状解析 (2008)
  • Author(s): 田矢史織, 荒川憲昭, 川崎博史, 宮城悦子, 平原史樹, 平野久
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] レドックスプロテオミスによる血管型NADPHオキシダーゼの標的タンパク質の探索 (2008)
  • Author(s): 野村文子, 荒川憲昭, 山中結子, 勝山真人, 川崎博史, 平野久
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] 川崎病のプロテオーム解析 (2008)
  • Author(s): 志村 直樹, 川崎 博史, 成戸 卓也, 今川 智之, 森 雅亮, 横田 俊平, 平野 久
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] シロイヌナズナカルス再分化におけるタンパク質リン酸化の変動 (2008)
  • Author(s): 風巻 玲子, 中村 浩規, 山中 結子, 平山 隆志, 平野 久
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] Proteomics for co- and post-translational modifications of the yeast 26S proteasome. (2008)
  • Author(s): 平野久
  • Organizer: Taiwan-Japan Proteomics Symposium
  • Place of Presentation: 台北
  • Year and Date: 2008-12-04
• [Presentation] Proteomics for CO- and post-translational modifications of the yeast 26S proteasome (2008)
  • Author(s): Hirano, H.
  • Organizer: Taiwan-Japan Proteomics Symposium
  • Place of Presentation: 台北
  • Year and Date: 2008-12-04
• [Presentation] ゲノムからプロテオームヘ (2008)
  • Author(s): 平野 久, 荒川憲昭, 増石有佑, 田矢志織, 山中結子, 宮城悦子, 平原史樹
  • Organizer: 第98回総会日本病理学会
  • Place of Presentation: 松山
  • Year and Date: 2008-11-20
• [Presentation] 中央アジア在来品種コムギにおける高分子量グルテニンサブユニットの変異 (2008)
  • Author(s): 寺沢 洋平, 高田 兼則, 河原 太八, 加藤 鎌司, 平野 久, 笹隈 哲夫, 笹沼 恒男
  • Organizer: 日本育種学会第114回講演会
  • Place of Presentation: 大津
  • Year and Date: 2008-10-12
• [Presentation] Proteomic analysis of serum from diabetic LEA/Sendai rats (2008)
  • Author(s): Takahashi E, Okamura T, Ikari K, Hirano H, Yasuda K, Kaburagi K.
  • Organizer: 17th Meeting of Methods in Protein Structure Analysis
  • Place of Presentation: 札幌
  • Year and Date: 2008-08-27
• [Presentation] Protein identification and quantification using a proteome database of liquid chromatography-mass spectrometric data. (2008)
  • Author(s): 川崎博史, 荒川憲昭, 平野久
  • Organizer: 17th Meeting of Methods in Protein Structure Analysis.
  • Place of Presentation: 札幌
  • Year and Date: 2008-08-27
• [Presentation] New potential therapeutic targets for ovarian clear cell carcinoma. (2008)
  • Author(s): 荒川憲昭, 増石有佑, 山中結子, 川崎博史, 宮城悦子, 平原史樹, 平野久
  • Organizer: 17th Meeting of Methods in Protein Structure Analysis
  • Place of Presentation: 札幌
  • Year and Date: 2008-08-27
• [Presentation] N^α-Terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae and its function. (2008)
  • Author(s): Kamita M, Kamp RM, Hirano H
  • Organizer: 17th Meeting of Methods in Protein Structure Analysis
  • Place of Presentation: 札幌
  • Year and Date: 2008-08-27
• [Presentation] Protein identification and quantification using a proteome database of liquid chromatography-mass spectrometric data (2008)
  • Author(s): Kawasaki, H., Arakawa, N., Hirano, H.
  • Organizer: The 17th Meeting of Methods in Protein Structure Analysis
  • Place of Presentation: 札幌
  • Year and Date: 2008-08-27
• [Presentation] New potential therapeutic targets for ovarian clear cell carcinoma (2008)
  • Author(s): Arakawa, N., Masuishi, Y., Yamanaka, Y., Kawasaki, H., Miyagi, E., Hirahara, F., Hirano, H.
  • Organizer: The 17th Meeting of Methods in Protein Structure Analysis
  • Place of Presentation: 札幌
  • Year and Date: 2008-08-27
• [Presentation] Nα-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae and its function (2008)
  • Author(s): Kamita, M., Kamp, R. M., Hirano, H.
  • Organizer: The 17th Meeting of Methods in Protein Structure Analysis
  • Place of Presentation: 札幌
  • Year and Date: 2008-08-27
• [Presentation] Proteomic analysis of serum from diabetic LEA/Sendai rats (2008)
  • Author(s): Takahashi, E., Okamura, T., Ikari, K., Hirano, H., Yasuda, K., Kaburagi, K.
  • Organizer: The 17th Meeting of Methods in Protein Structure Analysis
  • Place of Presentation: 札幌
  • Year and Date: 2008-08-27
• [Presentation] GH transgenic salmon proteomics (2008)
  • Author(s): Kurata, Y., Mori, T., Yamanaka, Y., Hirano, H.
  • Organizer: The 17th Meeting of Methods in Protein Structure Analysis
  • Place of Presentation: 札幌
  • Year and Date: 2008-08-27
• [Presentation] Identification and validation of ovarian cancer-associated proteins. (2008)
  • Author(s): Hirano H, Arakawa N, Kawasaki H., Masuishi Y, Takahashi E, Yahagi S, Yamanaka Y, Miyagi E, Hirahara F.
  • Organizer: 2nd Pacific Rim International Conference on Protein Science/4th Asian-Oceania Human Proteome Organization
  • Place of Presentation: ケアンズ
  • Year and Date: 2008-06-25
• [Presentation] Identification and validation of ovarian cancer-associated proteins (2008)
  • Author(s): Hirano, H., Arakawa, N., kawasaki, H., Masuishi, Y, Takahashi, E., Yahagi, S., Yamanaka, Y.,Miyagi, E., Hirahara
  • Organizer: The joint 2nd Pacific Rim International Conference on Protein Science and 4th Asian-Oceania Human Proteome Organization
  • Place of Presentation: ケアンズ
  • Year and Date: 2008-06-25
• [Presentation] Proteomic analysis for identification of therapeutic targets of ovarian clear cell carcinoma. (2008)
  • Author(s): 荒川憲昭, 増石有佑, 川崎博史, 宮城悦子, 平原史樹, 平野久
  • Organizer: 2nd Pacific Rim International Conference on Protein Science/4th Asian-Oceania Human Proteome Organization
  • Place of Presentation: ケアンズ
  • Year and Date: 2008-06-23
• [Presentation] プロテオーム研究の動向 (2008)
  • Author(s): 平野 久
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-11
• [Presentation] Bud32p複合体は出芽酵母二倍体において出芽マーカーの局在決定に関与している (2008)
  • Author(s): 加藤 悠, 川崎 博史, 平野 久
  • Organizer: 第8回日本蛋白質科学会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-10
• [Presentation] Proteomic analysis for identification of therapeutic targets of ovarian clear cell carcinoma (2008)
  • Author(s): Arakawa, N., Masuishi, Y., Kawasaki, H., Miyagi, E., Hirahara, F., Hirano, H.
  • Organizer: The joint 2nd Pacific Rim International Conference on Protein Science and 4th Asian-Oceania Human Proteome Organization
  • Place of Presentation: ケアンズ
  • Year and Date: 2008-06-03
• [Presentation] LEA/Sendaiラット血清のプロテオーム解析 (2008)
  • Author(s): 高橋 枝里, 岡村 匡史, 井狩 高平, 平野 久, 安田 和基, 鏑木 康志
  • Organizer: 第51回日本糖尿病学会年次学術集会
  • Place of Presentation: 東京
  • Year and Date: 2008-05-23
• [Presentation] Proteomic approach to discover biomarkers and therapeutic targets. (2008)
  • Author(s): 平野久
  • Organizer: 13th Joint Biophysics Conference
  • Place of Presentation: 台湾
  • Year and Date: 2008-05-21
• [Presentation] Proteomic approach to discover biomarkers and therapeutic targets. (2008)
  • Author(s): Hirano, H.
  • Organizer: The 13th Joint Biophysics Conference
  • Place of Presentation: 南投(台湾)
  • Year and Date: 2008-05-21
• [Book] タンパク質のアミノ酸配列と翻訳後修飾の分析,やさしい原理からはいるタンパク質化学実験法,タンパク質をみる-構造と挙動- 京都 (2009)
  • Author(s): 平野久
  • Publisher: 化学同人
• [Book] N-Terminal sequencing of N-terminally modified proteins. In: The Protein Protocols Handbook, New York (2009)
  • Author(s): Kamp K, Hirano H.
  • Publisher: Humana Press
• [Book] iTRAQ試薬を用いた疾患バイオマーカー探索,明日を拓く新次元プロテオミクス 東京 (2009)
  • Author(s): 木村弥生, 平野久
  • Publisher: 秀潤社
• [Book] やさしい原理からはいるタンパク質化学実験法 (2009)
  • Author(s): 平野 久
  • Publisher: 化学同人, 京都(印刷中)
• [Book] タンパク質の事典 東京 (2008)
  • Author(s): 平野久
  • Publisher: 朝倉書店
• [Book] タンパク質の事典 (2008)
  • Author(s): 平野 久
  • Publisher: 朝倉書店, 東京
• [Remarks] ホームページ
  • URL: http://proteome.sci.yokohama-cu.ac.jp
• [Patent(Industrial Property Rights)] 電気泳動で分離されたタンパク質を膜フィルターに転写し質量分析する方法 (2010)
  • Inventor(s): 平野久, 井野洋子
  • Filing Date: 2010-05-14