Structure-based Engineering of Enzymes Involved in the Biosynthesis of Secondary Metabolites
| Project/Area Number |
20310136
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | University of Shizuoka |
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Principal Investigator |
ABE Ikuro University of Shizuoka, 大学院・薬学系研究科, 教授 (40305496)
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| Co-Investigator(Renkei-kenkyūsha) |
KHONO Toshiyuki 三菱化学生命科学研究所, 主任研究員 (40416657)
SAITO Kazuki 千葉大学, 大学院・薬学研究院, 教授 (00146705)
KAWAHARA Nobuo 国立医薬品食品衛生研究所, 室長 (10224825)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2008: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
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| Keywords | 生合成 / 二次代謝酵素 / 結晶構造解析 / 酵素工学 / 物質生産 / 生合成工学 / ポリケタイド / 酵素 / 遺伝子 |
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| Research Abstract |
Type III polyketide synthases catalyze the biosynthesis of structurally diverse, biologically active plant polyophenols. On the basis of the X-ray crystal structures of the enzymes, we rationally designed and generated a set of site-directed mutant enzymes with novel and expanded catalytic functions. Further, by exploiting the promiscuous substrate specificities of the enzymes, we succeeded in production of an array of chemically and structurally divergent unnatural novel compounds.
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Report
(4 results)
Research Products
(64 results)
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[Journal Article] Alkylresorcinol Synthases from Sorghum bicolor Involved in The Biosynthesis of the Allelopathic Benzoquinone Sorgoleone.2010
Author(s)
D.Cook, A.M.Rimando, T.E.Clemente, F.E.Dayan, N.P.D.Nanayakkara, J.Schroder, Z.Pan, B.P.Noonan, M.Fishbein, I.Abe, S.O.Duke, S.R.Baerson
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Journal Title
Plant Cell 22
Pages: 867-887
Related Report
Peer Reviewed
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[Journal Article] Proc.Natl.Acad.Sci.USA2010
Author(s)
H.Morita, Y.Shimokawa, M.Tanio, R.Kato, H.Noguchi, S.Sugio, T.Kohno, I.Abe
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Journal Title
A Structure-Based Mechanism for Benzalacetone Synthase from Rheu m palmatum
Volume: 107
Pages: 669-673
Related Report
Peer Reviewed
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[Journal Article] Bioorg.Med.Chem.Lett.2010
Author(s)
H.Morita, K.Wanibuchi, R.Kato, S.Sugio, I.Abe
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Journal Title
Structure-Based Engineering of Benzalacetone Synthase
Volume: 20
Pages: 5099-5103
Related Report
Peer Reviewed
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