Generation of a New Class of Catalytic Antibodies based Directed Evolution
Project/Area Number |
20310138
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Living organism molecular science
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Research Institution | Osaka Prefecture University |
Principal Investigator |
IKUO Fujii Osaka Prefecture University, 理学系研究科, 教授 (70189984)
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Co-Investigator(Kenkyū-buntansha) |
TSUMURAYA Takeshi 大阪府立大学, 理学系研究科, 准教授 (00372855)
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Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
|
Keywords | 抗体酵素 / モノクローナル抗体 / 免疫学 / タンパク質 / 遷移状態アナログ / 抗原 / ハイブリドーマ / 免疫 / 進化分子工学 / ライブラリー |
Research Abstract |
In this study we have tried to generate a new class of catalytic antibodies by two ways, directed evolution and newly designed hapten immunization. We have developed a yeast surface-displayed library of catalytic antibodies showing a luciferase-like activity. In addition, we demonstrate a single antibody catalyzing multiple chemical transformations by the generation of antigen-combining site that function as an apoprotein for binding functionalized small non-protein components. Replacement of this cofactor with acidic and amino cofactors enabled the antibodies to catalyze β-elimination, decarboxylation and aldol reactions with large rate accelerations.
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Report
(4 results)
Research Products
(101 results)
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[Journal Article] Critical Contribution of Aromatic Rings to Specific Recognition of Polyether Rings by Antibody : The Case of Ciguatoxin CTX3C and Its Specific Antibody 1C92008
Author(s)
K. Tsumoto, A. Yokota, Y. Tanaka, M. Ui, T. Tsumuraya, I. Fujii, I. Kumagai, Y. Nagumo, H. Oguri, M. Inoue, M. Hirama
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Journal Title
J.Mol.Biol. 283
Pages: 12259-12266
Related Report
Peer Reviewed
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[Journal Article] Critical Contribution of Aromatic Rings to Specific Recognition of Polyether Rings by Antibody : The Case of Ciguatoxin CTX3C and Its Specific Antibody 1C92008
Author(s)
Kouhei Tsumoto, Akiko Yokota, Yoshikazu Tanaka, Mihoko Ui, Takeshi Tsumuraya, Ikuo Fujii, Izumi Kumagai, Yoko Nagumo, Hiroki Oguri, Masayuki Inoue, Masahiro Hirama
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Journal Title
Journal of Biological Chemistry 283
Pages: 12259-12266
Related Report
Peer Reviewed
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[Presentation] ホロ酵素型触媒抗体の分子設計2010
Author(s)
廣瀬仁孝, 石川文洋, 円谷健, 藤井郁雄
Organizer
第27回有機合成化学セミナー
Place of Presentation
シーサイドホテル舞子ビラ神戸(兵庫県)
Year and Date
2010-09-03
Related Report
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[Presentation] ホロ酵素型抗体酵素2009
Author(s)
石川文洋, 円谷健, 藤井郁雄
Organizer
2009年度酵素・補酵素研究会
Place of Presentation
奈良
Year and Date
2009-07-10
Related Report
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