| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Research Abstract |
In this study we have tried to generate a new class of catalytic antibodies by two ways, directed evolution and newly designed hapten immunization. We have developed a yeast surface-displayed library of catalytic antibodies showing a luciferase-like activity. In addition, we demonstrate a single antibody catalyzing multiple chemical transformations by the generation of antigen-combining site that function as an apoprotein for binding functionalized small non-protein components. Replacement of this cofactor with acidic and amino cofactors enabled the antibodies to catalyze β-elimination, decarboxylation and aldol reactions with large rate accelerations.
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