Phylogenetic positions and biomineralization of eggshell proteins of turtles

• Project/Area Number: 20340142
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Stratigraphy/Paleontology
• Research Institution: University of Tsukuba
• Principal Investigator: ENDO Kazuyoshi University of Tsukuba, 大学院・理学系研究科, 教授 (80251411)
• Co-Investigator(Kenkyū-buntansha): HIRAYAMA Ren 早稲田大学, 国際教養学術院, 教授 (00238396)
• Co-Investigator(Renkei-kenkyūsha): SARASHINA Isao 東京大学, 大学院・理学系研究科, 研究員 (90375444)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2009: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2008: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
• Keywords: 系統学 / 生体鉱物学 / カメ類 / 卵殻タンパク質 / 爬虫類 / バイオミネラリゼーション

Research Abstract

We performed immunological assays on the extracts from eggshells of reptiles and birds. The results support that the archosaurian affinity of turtles. Raman measurements showed that eggshells of turtles are aragonite, whereas those of snakes, lizards, crocodiles and birds are calcite. We examined the ion composition in the secretions of the oviducts of soft-shelled turtles and hens. Under both the conditions, not aragonite but calcite is expected to be formed, suggesting that the switch between the polymorphs may be controlled by proteins.

Research Products

• [Journal Article] Possible functions of Dpp in gastropod shell formation and shell coiling. (2011)
  • Author(s): Shimizu, K., Sarashina, I., Kagi, H., Endo, K.
  • Journal Title: Development, Genes and Evolution Volume: 印刷中(掲載確定)
  • Peer Reviewed
• [Journal Article] ネアンデルタール人のゲノム配列 (2011)
  • Author(s): 遠藤一佳
  • Journal Title: 遺伝 Volume: 65 Pages: 1-4
  • NAID: 40018824246
• [Journal Article] Artificial fertilization, early development and chromosome numbers in the brachiopod Lingula anatina. (2010)
  • Author(s): Nishizawa, A., Sarashina, I., Tsujimoto, Y., Iijima, M., Endo K.
  • Journal Title: Special Papers in Palaeontology Vol.84 Pages: 309-316
• [Journal Article] バイオミネラリゼーションの起源と進化 (2010)
  • Author(s): 遠藤一佳・更科功
  • Journal Title: 遺伝 Vol.64, No.4 Pages: 23-28
• [Journal Article] Artificial fertilization, early development and chromosome number in the brachiopod Lingula anatina (2010)
  • Author(s): Nishizawa, A., Sarashina, I., Tsujimoto, Y., Iijima, M., Endo, K.
  • Journal Title: Special Papers in Palaeontology (印刷中)
  • Peer Reviewed
• [Journal Article] バイオミネラリゼーションの起源と進化 (2010)
  • Author(s): 遠藤一佳・更科功
  • Journal Title: 遺伝 (印刷中)
• [Journal Article] Intraspecific variations in the ITS region of Recent radiolarians (2009)
  • Author(s): Ando, H., Kunitomo Y., Sarashina I., Iijima, M., Endo K., Sashida, K.
  • Journal Title: Earth Evolution Sciences 3 Pages: 37-44
  • NAID: 120001356327
• [Journal Article] Phylogenetic position of turtles among reptiles: evidence from immunological comparisons of eggshell matrices (2009)
  • Author(s): Nishi, M., Sarashina I., Iijima, M., Hirayama, R., Endo K.
  • Journal Title: Earth Evolution Sciences 3 Pages: 45-51
  • NAID: 120001356328
• [Journal Article] Phylogenetic position of turtles among reptiles: evidence from immuno logical comparisons of eggshell matrices. (2009)
  • Author(s): Nishi, M., Sarashina, I., Iijima, M., Hiravama, R. and Endo, K.
  • Journal Title: Earth Evolution Sciences 3 Pages: 45-51
  • Peer Reviewed
• [Journal Article] Dynamic expression patterns of vasa during embryogenesis in the cricket Gryllus bimaculatus. (2008)
  • Author(s): Mito T, Nakamura T, Sarashina I, Chang CC, Ogawa S, Ohuchi H, Noji S
  • Journal Title: Dev Genes Evol Vol.218, No.7 Pages: 381-387
• [Journal Article] Preservation of the shell matrix protein Dermatopontin in 1500-year-old land snail fossils from the Bonin islands. (2008)
  • Author(s): Sarashina I., Kunitomo Y., Chiba, S., Iijima, M., Endo K.
  • Journal Title: Organic Geochemistry 39 Pages: 1742-1746
  • NAID: 120007138453
• [Journal Article] Expression patterns of engrailed and dpp in the gastropod Lymnaea stagnalis. (2008)
  • Author(s): Iijima, M., Sarashina I., Takeuchi, T., Endo K.
  • Journal Title: Development, Genes and Evolution 218 Pages: 237-251
• [Journal Article] In vitro regulation of CaCO3 crystal polymorphism by the highly acidic molluscan shell protein Aspein. (2008)
  • Author(s): Takeuchi, T., Sarashina I., Iijima, M., Endo K.
  • Journal Title: FEBS Letters 582 Pages: 591-596
  • NAID: 120007138653
• [Journal Article] 軟体動物の貝殻タンパク質の進化 (2008)
  • Author(s): 更科功・遠藤一佳
  • Journal Title: 月刊地球号外 no.59 Pages: 103-109
• [Journal Article] 硬組織に含まれる基質タンパク質のinvivoにおける機能解析 (2008)
  • Author(s): 国友良樹・更科功・遠藤一佳
  • Journal Title: 月刊地球号外 No.59 Pages: 96-102
• [Presentation] Characterization of the shell matrix proteins of calcareous foraminifera. (2011)
  • Author(s): Isao Sarashina, Takashi Toyohuku, Kazuhiko Fujita, Masashi Tsuchiya, Kazuyoshi Endo, Hiroshi Kitazato
  • Organizer: Japan Geoscience Union International Symposium 2011
  • Place of Presentation: Makuhari Messe, Chiba, Japan
  • Year and Date: 2011-05-26
• [Presentation] 翼形類(二枚貝)における貝殻基質タンパク質アスペインの分子進化 (2011)
  • Author(s): 磯和幸延、更科功、遠藤一佳
  • Organizer: 日本地球惑星科学連合2011年大会
  • Place of Presentation: 幕張メッセ、千葉
  • Year and Date: 2011-05-26
• [Presentation] 翼形類(二枚貝)における貝殻基質タンパク質アスペインの分子進化 (2010)
  • Author(s): 磯和幸延、更科功、遠藤一佳
  • Organizer: 第5回バイオミネラリゼーションワークショップ
  • Place of Presentation: 東京大学農学部、東京
  • Year and Date: 2010-11-06
• [Presentation] 巻貝の貝殻形成におけるBMP2/4 の機能解析 (2010)
  • Author(s): 清水啓介、更科功、遠藤一佳
  • Organizer: 第5回バイオミネラリゼーションワークショップ
  • Place of Presentation: 東京大学農学部、東京
  • Year and Date: 2010-11-06
• [Presentation] In vitro functional analysis of molluscan shell matrix proteins. (2010)
  • Author(s): K.Endo
  • Organizer: Gordon Research Conference on biomineralization
  • Place of Presentation: ニコーロンドン,米国 招待講演
  • Year and Date: 2010-08-17
• [Presentation] Phylogeny estimation based on fossil protein sequences : a case of extinct land snails from the bonin islands (2010)
  • Author(s): Endo, K., Sarashina, I., Chiba, Sand Takao, T.
  • Organizer: International Paleontological Congress
  • Place of Presentation: ロンドン,連合王国
  • Year and Date: 2010-07-02
• [Presentation] モノアラガイの貝殻形成におけるBMP2/4の機能解析 (2010)
  • Author(s): 清水啓介、更科功、遠藤一佳
  • Organizer: 日本動物学会関東支部第62回大会
  • Place of Presentation: 筑波大学、つくば
  • Year and Date: 2010-03-13
• [Presentation] Artificial fertilization in Lingula anatina : a portal to brachiopod developmental genetics. (2010)
  • Author(s): K.Endo
  • Organizer: 6th International Brachiopod Congress
  • Place of Presentation: メルボルン
  • Year and Date: 2010-02-05
• [Presentation] Molecular evolution of the shell matrix protein Aspein in pterioid bivalves (2010)
  • Author(s): 磯和幸延, 更科功, 遠藤一佳
  • Organizer: International Symposium on Biodiversity Science 2010 "Genome, Evolution and Environment"
  • Place of Presentation: Nagoya City University, Nagoya, Japan
• [Presentation] 化石タンパク質によるカタツムリの系統推定 (2010)
  • Author(s): 更科功、千葉聡、遠藤一佳
  • Organizer: 日本古生物学会2010年年会
  • Place of Presentation: 筑波大学、つくば
• [Presentation] 石灰質有孔虫の殻内タンパク質の検討 (2010)
  • Author(s): 更科功、豊福高志、藤田和彦、遠藤一佳
  • Organizer: 日本古生物学会2010年年会
  • Place of Presentation: 筑波大学、つくば
• [Presentation] 翼形類(二枚貝)における貝殻基質タンパク質アスペインの分子進化 (2010)
  • Author(s): 磯和幸延、更科功、遠藤一佳
  • Organizer: 日本古生物学会2010年年会
  • Place of Presentation: 筑波大学、つくば
• [Presentation] 「カルサイト-アラゴナイト問題」の一つの答 (2010)
  • Author(s): 遠藤一佳、竹内猛、磯和幸延、更科功
  • Organizer: 日本地球惑星科学連合2010年大会
  • Place of Presentation: 幕張メッセ、千葉
• [Presentation] Structure, function and evolution of shell matrix proteins in the pearloyster Pinctada fucata (2009)
  • Author(s): K.Endo, T.Takeuchi, Y.Isowa, I.Sarashina
  • Organizer: International Symposium on Marine Genomics
  • Place of Presentation: 那覇市ホテル海邦
  • Year and Date: 2009-12-16
• [Presentation] 下田沖放散虫類のITS 領域における種内変異 (2009)
  • Author(s): 安藤寛・国友良樹・更科功・飯島実・遠藤一佳・指田勝男
  • Organizer: 第10回放散虫研究集会
  • Place of Presentation: 山口大学
  • Year and Date: 2009-03-20
• [Presentation] 腕足動物ミドリシャミセンガイの染色体数と初期発生-人工授精法- (2009)
  • Author(s): 西澤暁子・更科功・辻本善信・飯島実・遠藤一佳
  • Organizer: 日本古生物学会第158回例会
  • Place of Presentation: 琉球大学
  • Year and Date: 2009-01-31
• [Presentation] 卵殻タンパク質を用いたカメ類の系統学的研究 (2009)
  • Author(s): 西真樹子・更科功・遠藤一佳・平山廉
  • Organizer: 日本古生物学会第158回例会
  • Place of Presentation: 琉球大学
  • Year and Date: 2009-01-31
• [Presentation] Structure, function and evolution of shell matrix proteins in the pearl oyster Pinctada fucata. (2009)
  • Author(s): Kazuyoshi Endo, Takeshi Takeuchi, Yukinobu Isowa, Isao Sarashina
  • Organizer: International Symposium on Marine Genomics
  • Place of Presentation: Hotel Southern Plaza Kaiho, Naha, Okinawa, Japan
• [Presentation] 遺伝子配置による多細胞動物の系統復元:傾向と対策 (2009)
  • Author(s): 遠藤一佳・更科功
  • Organizer: 日本地球惑星科学連合2009年大会
  • Place of Presentation: 幕張メッセ、千葉
• [Presentation] 巻貝における新規炭酸脱水酵素と軟体動物の貝殻の進化 (2009)
  • Author(s): 更科功・遠藤一佳
  • Organizer: 日本地球惑星科学連合2009年大会
  • Place of Presentation: 幕張メッセ、千葉
• [Presentation] 翼形類における貝殻タンパク質アスペインの分子進化 (2009)
  • Author(s): 磯和幸延・横尾直樹・更科功・遠藤一佳
  • Organizer: 日本地球惑星科学連合2009年大会
  • Place of Presentation: 幕張メッセ、千葉
• [Presentation] 翼形類(二枚貝)における貝殻基質タンパク質Aspeinの分子進化 (2009)
  • Author(s): 磯和幸延、横尾直樹、更科功、遠藤一佳
  • Organizer: 東京大学海洋研究所共同利用研究集会「バイオミネラリゼーションと石灰化-遺伝子から地球環境まで-」
  • Place of Presentation: 東京大学海洋研究所、東京
• [Presentation] 軟体動物の貝殻タンパク質Nacreinの進化 (2008)
  • Author(s): 更科功
  • Organizer: 第3回バイオミネラリゼーションワークショップ
  • Place of Presentation: 東京大学農学部、東京
  • Year and Date: 2008-12-13
• [Presentation] 翼形類における貝殻基質タンパク質Aspeinの分子進化プロセス (2008)
  • Author(s): 磯和幸延・更科功・遠藤一佳
  • Organizer: 第3回バイオミネラリゼーションワークショップ
  • Place of Presentation: 東京大学
  • Year and Date: 2008-12-13
• [Presentation] 貝殻基質タンパク質に見られるダイナミックな適応進化 (2008)
  • Author(s): 遠藤一佳・更科功
  • Organizer: 日本進化学会第10回年会
  • Place of Presentation: 東京大学,駒場,東京
  • Year and Date: 2008-08-24
• [Presentation] 貝殻基質タンパク質の分子進化 (2008)
  • Author(s): 遠藤一佳・更科功
  • Organizer: 日本地球惑星科学連合2008年大会
  • Place of Presentation: 幕張メッセ,千葉
  • Year and Date: 2008-05-27
• [Presentation] Multiple origins of animal skeletons and dynamic adaptive evolution documented by molluscan shell matrix proteins. (2008)
  • Author(s): 遠藤一佳, 更科功
  • Organizer: International Symposium "From Genome to Snowball Earth,Metazoan Evolution and HabitablePlanets: Multidisciplinary Relations,"
  • Place of Presentation: MIRAIKAN, Odaiba, Tokyo
• [Presentation] Expression and function of the unusually acidic shell matrix protein Aspein. (2008)
  • Author(s): Endo k., Takeuchi, T., Kunitomo Y., Sarashina I.
  • Organizer: Gordon Research Conference on biomineralization
  • Place of Presentation: Colby-Sawyer College, New London, New Hampshire, USA(ポスター)
• [Book] 進化の運命孤独な宇宙の必然としての人間 (2010)
  • Author(s): サイモン・コンウェイ=モリス(著)、遠藤一佳、更科功(訳)
  • Total Pages: 724
  • Publisher: 講談社、東京
• [Book] 進化の運命 (2010)
  • Author(s): サイモン・コンウェイモリス(遠藤一佳・更科功訳)
  • Total Pages: 724
  • Publisher: 講談社