Versatile Molecular Devices Based on Functional DNA and PNA Complexes

• Project/Area Number: 20350038
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Kumamoto University
• Principal Investigator: IHARA Toshihiro 熊本大学, 大学院・自然科学研究科, 教授 (40253489)
• Co-Investigator(Kenkyū-buntansha): SAKURAI Toshihiko 鳥取大学, 工学部, 准教授 (10332868)
• Co-Investigator(Renkei-kenkyūsha): HAYASHIDA Osamu 福岡大学, 理学部, 教授 (20231532)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2010: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2008: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
• Keywords: DNAコンジュゲート / 協同性 / 化学センサー / バイオセンサー / 光化学 / 錯生成 / ハイブリダイゼーション / 機能性核酸 / シクロデキストリン / シグナル化学増幅 / DNAzyme / 生体分析化学 / オリゴヌクレオチド / PNA / 電気化学 / 蛍光 / 金属イオン / ターピリジン / 金属錯体 / 融解曲線 / 高次構造の制御 / アントラセン / 光二量化 / 三本鎖DNA / テロメア / 質量分析

Research Abstract

The research aims at the development of the DNA and PNA-based functional modules and their integrated flexible system for chemical or physical signal amplification and transduction. DNA conjugates carryingβ-cyclodextrin, terpyridine, and anthracene were prepared as functional modules for fluorescent SNP analysis, metallo-regulation of DNA functions, and reversible photo-regulation of DNA-based nanostructures, respectively.

Research Products

• [Journal Article] Photochemically Relevant DNA-based Molecular Systems Enabling Chemical and Signal Transductions and Their Analytical Applications (2012)
  • Author(s): T. Ihara, Y. Kitamura
  • Journal Title: J. Photochem. Photobiol. C Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] スプリット型プローブの協同的錯体形成を利用するDNAの認識及び検出 (2012)
  • Author(s): 井原敏博, 北村裕介
  • Journal Title: 分析化学 Volume: 61巻 Pages: 193-206
  • Peer Reviewed
• [Journal Article] DNA Analysis Based on the Local Structural Disruption on the Duplexes Carrying a Luminous Lanthanide Complex (2011)
  • Author(s): T. Ihara, Y. Kitamura, Y. Tsujimura, A. Jyo
  • Journal Title: Anal. Sci Volume: 27巻 Pages: 585-590
  • NAID: 10028289066
  • Peer Reviewed
• [Journal Article] Electrochemical Signal Modulation in Homogeneous Solutions Using the Formation of an Inclusion Complex between Ferrocene andβ-cyclodextrin on DNA Scaffold (2011)
  • Author(s): T. Ihara, T. Wasano, R. Nakatake, P. Arslan, A. Futamura, A. Jyo
  • Journal Title: Chem. Commun Volume: 47巻 Pages: 12388-12390
  • Peer Reviewed
• [Journal Article] DNA Analysis Based on the Local Structural Disruption to the Duplexes Carrying a Luminous Lanthanide Complex (2011)
  • Author(s): T.Ihara, Y.Kitamura, Y.Tsujimura, A.Jyo
  • Journal Title: Analytical Sciences Volume: 27 Issue: 6 Pages: 585
  • DOI: 10.2116/analsci.27.585
  • NAID: 10028289066
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed
• [Journal Article] Enhanced Trace Phosphate Removal from Water by Zirconium (IV) Loaded Fibrous Adsorbent (2011)
  • Author(s): Md.R.Awual, A.Jyo, T.Ihara, N.Seko, M.Tamada, K.T.Lim
  • Journal Title: Water Research Volume: 45 Issue: 15 Pages: 4592-4600
  • DOI: 10.1016/j.watres.2011.06.009
  • Peer Reviewed
• [Journal Article] Electrochemical Signal Modulation in Homogeneous Solutions Using the Formation of an Inclusion Complex between Ferrocene and β-cyclodextrin on DNA Scaffold (2011)
  • Author(s): T.Ihara, T.Wasano, R.Nakatake, P.Arslan, A.Futamura, A.Jyo
  • Journal Title: Chemical Communications Volume: 47 Issue: 45 Pages: 12388-12390
  • DOI: 10.1039/c1cc15365j
  • Peer Reviewed
• [Journal Article] Reversible Circularization of an Anthracene-modified DNA Conjugate through Bimolecular Triplex Formation and Its Analytical Application (2010)
  • Author(s): P. Arslan, A. Jyo, T. Ihara
  • Journal Title: Org. Biomol. Chem. Volume: 8巻 Pages: 4843-4848
  • Peer Reviewed
• [Journal Article] Reversible Circularization of an Anthracene-modified DNA Conjugate through Bimolecular Triplex Formation and Its Analytical Application (2010)
  • Author(s): P.ArsIan, A.Jyo, T.Ihara
  • Journal Title: Org. Biomol. Chem. 8 Pages: 4843-4848
• [Journal Article] Reversible circularization of an anthracene-modified DNA conjugate through bimolecular triplex formation and its analytical application (2010)
  • Author(s): Pelin Arslan
  • Journal Title: Organic Biomolecular Chemistry Volume: 8 Pages: 4843-4848
  • Peer Reviewed
• [Journal Article] Cooperative DNA Probing Using aβ-Cyclodextrin. DNA Conjugates and a Nucleobase-specific Fluorescent Ligand (2009)
  • Author(s): T. Ihara, A. Uemura, A. Futamura, M. Shimizu, N. Baba, S. Nishizawa, N. Teramae, A. Jyo
  • Journal Title: J. Am. Chem. Soc. Volume: 131巻 Pages: 1386-1387
  • Peer Reviewed
• [Journal Article] Anthracene-DNA Conjugates as Building Blocks of Designed DNA Structures Constructed by Photochemical Reactions (2009)
  • Author(s): M. Mukae, T. Ihara, M. Tabara, A. Jyo
  • Journal Title: Org. Biomol. Chem. Volume: 7巻 Pages: 1349-1354
  • Peer Reviewed
• [Journal Article] Silver Ion Unusually Stabilizes the Structure of a Parallel-Motif DNA Triplex (2009)
  • Author(s): T. Ihara, T. Ishii, N. Araki, A. W. Wilson, A. Jyo
  • Journal Title: J. Am. Chem. Soc. Volume: 131巻 Pages: 3826-3827
  • Peer Reviewed
• [Journal Article] Photodimerization of Anthracenes Using a DNA Template and Its Analytical Applications (2009)
  • Author(s): M. Mukae, T. Ihara, M. Tabara, P. Arslan, A. Jyo
  • Journal Title: Supramol. Chem. Volume: 21巻 Pages: 292-295
  • Peer Reviewed
• [Journal Article] DNA Conjugates Bearing a Ferrocenyl Group in Backbone and Their Electrochemical Behavior (2009)
  • Author(s): T. Ihara, D. Sasahara, M. Shimizu, A. Jyo
  • Journal Title: Supramol. Chem. Volume: 21巻 Pages: 207-217
  • Peer Reviewed
• [Journal Article] Efficient Guest Inclusion byβ-Cyclodextrin Attached to the Ends of DNA Oligomers upon Hybridization to Various DNA Conjugates (2009)
  • Author(s): A. Kuzuya, T. Ohnishi, T. Wasano, S. Nagaoka, J. Sumaoka, T. Ihara, A. Jyo, M. Komiyama
  • Journal Title: Bioconjugate Chem. Volume: 20巻 Pages: 1643-1649
  • Peer Reviewed
• [Journal Article] Cooperative DNA Probing Using a β-Cyclodextrin-DNA Conjugates and a Nucleobase-specific Fluorescent Ligand (2009)
  • Author(s): T.Ihara, A.Uemura, A.Futamura, M.Shimizu, N.Baba, S.Nishizawa, N.Teramae, A.Jyo
  • Journal Title: J. Am. Chem. Soc. 131 Pages: 1386-1387
• [Journal Article] Anthracene-DNA Conjugates as Building Blocks of Designed DNA Structures Constructed by Photochemical Reactions (2009)
  • Author(s): M.Mukae, T.Ihara, M.Tabara, A.Jyo
  • Journal Title: Org. Biomol. Chem. 7 Pages: 1349-1354
• [Journal Article] Silver Ion Unusually Stabilizes the Structure of a Parallel-Motif DNA Triplex (2009)
  • Author(s): T.Ihara, T.Ishii, N.Araki, A.W.Wilson, A.Jyo
  • Journal Title: J. Am. Chem. Soc. 131 Pages: 3826-3827
• [Journal Article] Efficient Guest Inclusion by β-Cyclodextrin Attached to the Ends of DNA Oligomers upon Hybridization to Various DNA Conjugates (2009)
  • Author(s): A.Kuzuya, T.Ohnishi, T.Wasano, S.Nagaoka, J.Sumaoka, T.Ihara, A.Jyo, M.Komiyama
  • Journal Title: Bioconjugate Chem. 20 Pages: 1643-1649
• [Journal Article] Cooperative DNA Probing Using a β-Cyclodextrin-DNA Conjugates and a Nucleobase-specific Fluorescent Ligand (2009)
  • Author(s): Toshihiro Ihara
  • Journal Title: Journal of the American Chemical Society 131 Pages: 1386-1387
  • Peer Reviewed
• [Journal Article] Anthracene-DNA Conjugates as Building Blocks of Designed DNA Structures Constructed by Photochemical Reactions (2009)
  • Author(s): Motoko Mukae
  • Journal Title: Organic Biomolecular Chemistry 7 Pages: 1349-1354
  • Peer Reviewed
• [Journal Article] Silver Ion Unusually Stabilizes the Structure of a Parallel-Motif DNA Triplex (2009)
  • Author(s): Toshihiro Ihara
  • Journal Title: Journal of the American Chemical Society 131 Pages: 3826-3827
  • Peer Reviewed
• [Journal Article] Photodimerization of Anthracenes Using a DNA Template and Its Analytical Applications (2009)
  • Author(s): Motoko Mukae
  • Journal Title: Supramolecular Chemistry 21 Pages: 292-295
  • Peer Reviewed
• [Journal Article] DNA Conjugates Bearing a Ferrocenyl Group in Backbone and Their Electrochemical Behavior (2009)
  • Author(s): Toshihiro Ihara
  • Journal Title: Supramolecular Chemistry 21 Pages: 207-217
  • Peer Reviewed
• [Journal Article] Interaction of Silver Ion with CG.C^+ Base Triplets in DNA Triplex (2009)
  • Author(s): Toshihiro Ihara
  • Journal Title: Nucleic Acids Research, Supplement Pages: 19-20
  • Peer Reviewed
• [Journal Article] Efficient Guest Inclusion by β-Cyclodextrin Attached to the Ends of DNA Oligomers upon Hybridization to Various DNA Conjugates (2009)
  • Author(s): Akinori Kuzuya
  • Journal Title: Bioconjugate Chemistry 20 Pages: 1643-1649
  • Peer Reviewed
• [Journal Article] The Effect of Local Structural Disruption on the Yield of Photochemical Ligation between Anthracene-Oligonucleotide Conjugates (2008)
  • Author(s): P. Arslan, T. Ihara, M. Motoko, A. Jyo
  • Journal Title: Anal. Sci. Volume: 24巻 Pages: 173-176
  • NAID: 10020143897
  • Peer Reviewed
• [Journal Article] Template-Directed Formation of Luminescent Lanthanide Complexes : Versatile Tools for Colorimetric Identification of Single Nucleotide Polymorphism (2008)
  • Author(s): Y. Kitamura, T. Ihara, Y. Tsujimura, Y. Osawa, D. Sasahara, M. Yamamoto, K. Okada, M. Tazaki, A. Jyo
  • Journal Title: J. Inorg. Biochem. Volume: 102巻 Pages: 1921-1931
  • Peer Reviewed
• [Journal Article] Metallo-regulation of Triple Helix Formation by Control of the Loop Conformation (2008)
  • Author(s): T. Ihara, Y. Sato, H. Shimada, A. Jyo
  • Journal Title: Nucleos. Nucleot. Nucl. Volume: 27巻 Pages: 1084-1096
  • Peer Reviewed
• [Journal Article] The Effect of Local Structural Disruption on the Yield of Photochemical Ligation between Anthracene-Oligonucleotide Conjugates (2008)
  • Author(s): Arslan, Pelin
  • Journal Title: Analytical Sciences 24 Pages: 173-176
  • Peer Reviewed
• [Journal Article] Template-Directed Formation of Luminescent Lanthanide Complexes : Versatile Tools for Colorimetric Identification of Single Nucleotide Polymorphism (2008)
  • Author(s): Kitamura, Yusuke
  • Journal Title: Journal of Inorganic Biochemistry 102 Pages: 1921-1931
  • Peer Reviewed
• [Journal Article] Metallo-regulation of Triple Helix Formation by Control of the Loop Conformation (2008)
  • Author(s): Ihara, Toshihiro
  • Journal Title: Nucleosides, Nucleotides, and Nucleic Acids 27 Pages: 1084-1096
  • Peer Reviewed
• [Presentation] 核酸上でのデザインされた特異反応およびその分析化学的応用 (2012)
  • Author(s): 井原敏博
  • Organizer: 第20回アンチセンスシンポジウム
  • Place of Presentation: 甲南大学,神戸市
  • Year and Date: 2012-12-02
• [Presentation] Electrochemical Study of DNA Hybridization in Homogeneous Solutions (2011)
  • Author(s): T. Ihara, T. Wasano, R. Nakatake, A. Futamura, A. Jyo
  • Organizer: The 38th International Symposium on Nucleic Acid Chemistry, ISNAC2011
  • Place of Presentation: 北海道大学, Sapporo
  • Year and Date: 2011-11-09
• [Presentation] Rational regulation of nucleic acid structures by specific formation of metal complexes (2011)
  • Author(s): T. Ihara
  • Organizer: The 14th Asian Chemical Congress
  • Place of Presentation: Bangkok,タイ王国
  • Year and Date: 2011-09-07
• [Presentation] Rational regulation of nucleic acid structures by specific formation of metal complexes (2011)
  • Author(s): T.Ihara
  • Organizer: The 14th Asian Chemical Congress
  • Place of Presentation: The Queen Sirikit National Convention Center, Bangkok(招待講演)
  • Year and Date: 2011-09-07
• [Presentation] 機能性核酸複合体のプログラミングと分析化学的応用 (2011)
  • Author(s): 井原敏博
  • Organizer: 中央大学理工学研究科学術講演会
  • Place of Presentation: 中央大学,東京
  • Year and Date: 2011-07-15
• [Presentation] 機能性核酸複合体のプログラミングと分析化学的応用 (2011)
  • Author(s): 井原敏博
  • Organizer: 中央大学理工学研究科学術講演会
  • Place of Presentation: 中央大学理工学部,東京都(招待講演)
  • Year and Date: 2011-07-15
• [Presentation] DNA probing by cooperative luminous complex formation on the target (2010)
  • Author(s): T. Ihara, Y. Kitamura, A. Uemura, N. Baba, S. Nishizawa, N. Teramae, A. Jyo
  • Organizer: PacifiChem 2010
  • Place of Presentation: Honolulu,アメリカ合衆国
  • Year and Date: 2010-12-16
• [Presentation] Cooperative DNA Probing Using-Cyclodextrin-modified DNA and Nucleobase-specific Fluorescent Ligand (2010)
  • Author(s): A. Futamura, A Uemura, A. Jyo, N. Baba, S. Nishizawa, N. Teramae, T. Ihara
  • Organizer: PacifiChem 2010
  • Place of Presentation: Honolulu,アメリカ合衆国
  • Year and Date: 2010-12-16
• [Presentation] Synthesis of DNA Conjugate for Photo-triggered New Chemical Ligation (2010)
  • Author(s): T. Hirayama, A. Jyo, T. Ihara
  • Organizer: PacifiChem 2010
  • Place of Presentation: Honolulu,アメリカ合衆国
  • Year and Date: 2010-12-16
• [Presentation] DNA probing by cooperative luminous complex formation on the target (2010)
  • Author(s): T.Ihara, Y., Y.Kitamura, A.Uemura, N.Baba, S.Nishizawa, N.Teramae, A.Jyo
  • Organizer: PacifiChem 2010
  • Place of Presentation: Honolulu招待講演
  • Year and Date: 2010-12-16
• [Presentation] DNA probing by cooperative luminous complex formation on the target (2010)
  • Author(s): Toshihiro Ihara
  • Organizer: The International Chemical Congress of Pacific Basin Societies (Pacifichem 2010)
  • Place of Presentation: Convention Center, Honolulu
  • Year and Date: 2010-12-16
• [Presentation] 核酸上でのデザインされた特異反応およびその分析化学的応用 (2010)
  • Author(s): 井原敏博
  • Organizer: 第20回アンチセンスシンポジウム
  • Place of Presentation: 神戸市招待講演
  • Year and Date: 2010-12-02
• [Presentation] Rational Construction of Luminous Structures on DNA Scaffold for Analytical Applications (2010)
  • Author(s): T.Ihara, A.Futamura, A.Uemura, Y.Kitamura, A.Jyo, Y.Sato, S.Nishizawa, N.Teramae
  • Organizer: The 19th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids (IRTXIX)
  • Place of Presentation: Lyon
  • Year and Date: 2010-08-30
• [Presentation] Rational Construction of Luminous Structures on DNA Scaffold for Analytical Applications (2010)
  • Author(s): T. Ihara, A. Futamura, A. Uemura, Y. Kitamura, A. Jyo, Y. Sato, S. Nishizawa, N. Teramae
  • Organizer: The 19th International Roundtable on Nucleosides, Nucleotides and Nucleic Acids(IRTXIX)
  • Place of Presentation: Lyon,フランス
  • Year and Date: 2010-08-29
• [Presentation] 機能分子を協調させて核酸を検出する (2010)
  • Author(s): 井原敏博
  • Organizer: 奈良先端科学技術大学院大学物質創成化学特講
  • Place of Presentation: 奈良先端大学院大学,奈良市
  • Year and Date: 2010-02-10
• [Presentation] Interaction of silver ion with CG. C^+base triplets in DNA triplex (2009)
  • Author(s): T. Ihara, T. Ishii, A. Jyo
  • Organizer: The 6th International Symposium on Nucleic Acids Chemistry 2009(6ISNAC2009)
  • Place of Presentation: 高山文化会館, Takayama
  • Year and Date: 2009-09-28
• [Presentation] Interaction of silver ion with CG.C^+ base triplets in DNA triplex (2009)
  • Author(s): T.Ihara, T.Ishii, A.Jyo
  • Organizer: The 6th International Symposium on Nucleic Acids Chemistry 2009 (6ISNAC2009)
  • Place of Presentation: Takayama
  • Year and Date: 2009-09-28
• [Presentation] DNA Probing Using Luminous Complexes Assembled on the Targets (2009)
  • Author(s): T. Ihara
  • Organizer: 2nd Switzerland-Japan Biomolecular Chemistry Symposium(SJBCS 2009)
  • Place of Presentation: 東京大学, Tokyo
  • Year and Date: 2009-09-11
• [Presentation] DNA Probing Using Luminous Complexes Assembled on the Targets (2009)
  • Author(s): T.Ihara
  • Organizer: 2nd Switzerland-Japan Biomolecular Chemistry Symposium (SJBCS 2009)
  • Place of Presentation: Tokyo招待講演
  • Year and Date: 2009-09-11
• [Presentation] DNA Probing Using Luminous Complexes Assembled on the Targets (2009)
  • Author(s): Toshihiro Ihara
  • Organizer: 2nd Switzerland-Japan Biomolecular Chemistry Symposium 2009
  • Place of Presentation: 東京大学
• [Presentation] 核酸上での分子間相互作用を利用したシグナル変換 (2008)
  • Author(s): 井原敏博
  • Organizer: 第11回生命化学研究会-生命化学をシステムで捉えたら-
  • Place of Presentation: ホテル水上館,群馬県利根郡みなかみ町
  • Year and Date: 2008-11-28
• [Presentation] スプリットプローブの形成する希土類錯体の発光を利用した遺伝子検出 (2008)
  • Author(s): 井原敏博
  • Organizer: 第58回錯体化学討論会(希土類錯体:未踏配位化学の探求と機能創成)
  • Place of Presentation: 金沢大学,金沢市
  • Year and Date: 2008-09-20
• [Presentation] Efficient Circular DNA Formation by Photochemical Ligation on the Bimolecular Triplex Consisting of Homopyrimidine Template and Anthracene-modified Oligonucleotide Conjugate (2008)
  • Author(s): P. Arslan, T. Ihara, A Jyo
  • Organizer: Joint Symposium of 18^<th> International Roundtable on Nucleosides, Nucleotides and Nucleic Acids and 35^<th> International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: 京都国立国際会館, Kyoto
  • Year and Date: 2008-09-10
• [Presentation] 核酸上での分子間相互作用を利用したシグナル変換 (2008)
  • Author(s): 井原敏博
  • Organizer: 日本分析化学会第57年会
  • Place of Presentation: 福岡大学,福岡市
  • Year and Date: 2008-09-10
• [Presentation] Photodimerization of Anthracene Using DNA Template and Its Analytical Application (2008)
  • Author(s): M. Mukae, T. Ihara, M. Tabara, P. Arslan, A. Jyo
  • Organizer: International Symposium on Macrocyclic and Supramolecular Chemistry 2008(ISMSC2008)
  • Place of Presentation: Las Vegas,アメリカ合衆国
  • Year and Date: 2008-07-17
• [Presentation] Electrochemical Gene Analysis Based on Cooperative Interaction of DNA Conjugates (2008)
  • Author(s): T. Ihara, T. Wasano, A. Uemura, D. Sasahara, M. Shimizu, A. Jyo
  • Organizer: International Symposium on Macrocyclic and Supramolecular Chemistry 2008(ISMSC2008)
  • Place of Presentation: Las Vegas,アメリカ合衆国
  • Year and Date: 2008-07-17
• [Presentation] 機能化DNAの協同性を利用した核酸の認識及び検出 (2008)
  • Author(s): 井原敏博
  • Organizer: 第45回化学関連支部合同九州大会
  • Place of Presentation: 北九州国際会議場,北九州市
  • Year and Date: 2008-07-05
• [Presentation] 核酸を足場とした超分子設計-計測系への応用- (2008)
  • Author(s): 井原敏博
  • Organizer: 第24回緑陰セミナー札幌
  • Place of Presentation: 国営滝野すずらん丘陵公園青少年自然の家,札幌市
  • Year and Date: 2008-06-29
• [Presentation] プローブを協調させてDNAを認識・分析する (2008)
  • Author(s): 井原敏博
  • Organizer: 大阪大学大学院薬学研究科特別講演会
  • Place of Presentation: 大阪大学,吹田市
  • Year and Date: 2008-05-16
• [Presentation] 核酸上での分子間相互作用を利用したシグナル変換 (2008)
  • Author(s): 井原, 敏博
  • Organizer: 第11回生命化学研究会
  • Place of Presentation: 群馬県水上町
• [Book] 核酸とカチオンの特異な相互作用、CSJカレントレビュー核酸化学のニュートレンド (2011)
  • Author(s): 井原敏博, 他
  • Publisher: 化学同人
• [Book] 基礎教育シリーズ分析化学(基礎編) (2011)
  • Author(s): 井原敏博, 他
  • Publisher: 東京教学社
• [Book] 基礎教育シリーズ分析化学(機器分析編) (2011)
  • Author(s): 井原敏博, 他
  • Publisher: 東京教学社
• [Book] CSJカレントレビュー核酸化学のニュートレンド(核酸とカチオンの特異な相互作用) (2011)
  • Author(s): 井原敏博(共同執筆)
  • Publisher: 化学同人 印刷中
• [Book] 未来材料 10(協同的分子認識に基づく核酸プロービング) (2010)
  • Author(s): 井原敏博
  • Publisher: NTS Inc.
• [Book] 生体機能関連化学部会研究レター 25(核酸上でのデザインされた特異反応およびその分析化学的応用) (2010)
  • Author(s): 井原敏博
  • Publisher: 日本化学会生体機能関連部会
• [Book] 基礎教育シリーズ 分析化学 基礎編 (2010)
  • Author(s): 本水昌二
  • Total Pages: 302
  • Publisher: 東京教学社
• [Book] 基礎教育シリーズ 分析化学 機器分析編 (2010)
  • Author(s): 本水昌二
  • Total Pages: 191
  • Publisher: 東京教学社
• [Book] 基礎教育シリーズ新版分析化学実験 (2008)
  • Author(s): 井原敏博, 他
  • Publisher: 東京教学社
• [Remarks]
• [Remarks]
  • URL: http://133.95.131.186/~toshi/
• [Remarks]
  • URL: http://133.95.131.186/~toshi/
• [Patent(Industrial Property Rights)] Method of Electrochemically Detecting Nucleic Acid with the Use of DNA Conjugate (2009)
  • Inventor(s): 井原敏博
  • Industrial Property Rights Holder: 熊本大学
  • Filing Date: 2009-03-10
  • Overseas
• [Patent(Industrial Property Rights)] 金属イオンにより核酸の多重構造を安定化させる方法 (2009)
  • Inventor(s): 井原敏博
  • Industrial Property Rights Holder: 熊本大学
  • Filing Date: 2009-02-16
• [Patent(Industrial Property Rights)] Method of Electrochemically Detecting Nucleic Acid with the Use of DNA Conjugate (2009)
  • Inventor(s): 井原敏博
  • Industrial Property Rights Holder: 熊本大学
  • Filing Date: 2009-03-10
• [Patent(Industrial Property Rights)] 金属イオンにより核酸の多重構造を安定化させる方法 (2009)
  • Inventor(s): 井原敏博
  • Industrial Property Rights Holder: 熊本大学
  • Filing Date: 2009-02-16
• [Patent(Industrial Property Rights)] DNAコンジュゲートを利用した核酸の電気化学的検出法 (2008)
  • Inventor(s): 井原敏博
  • Industrial Property Rights Holder: 熊本大学
  • Filing Date: 2008-06-27
• [Patent(Industrial Property Rights)] DNAコンジュゲートを利用した核酸の電気化学的検出法 (2008)
  • Inventor(s): 井原敏博
  • Industrial Property Rights Holder: 熊本大学
  • Filing Date: 2008-06-27