| Budget Amount *help |
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2010: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2009: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2008: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
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| Research Abstract |
Restriction of the number of endogenous tRNAs in protein synthesis system causes "empty codons" in the genetic code dictionary. If we can create a tRNA that reads "an empty codon" and accepts an unnatural amino acid, it means that we develop an artificial genetic code dictionary. Here, I purified 21 tRNAs from Escherichia coli, and made a minimal tRNA set by remixing an individual tRNA. Actually, yellow fluorescent protein could be synthesized in PURE system with a minimal tRNA set and a chemically synthesized gene having minimal codons. It indicates that proteins having several unnatural amino acids can be effectively synthesized in the future.
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