Control of Orientation and Shape of Adult Stem Cell on Base Surface by Negative-Ion Pattern Implantation and Induction of The Differentiation
| Project/Area Number |
20360018
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thin film/Surface and interfacial physical properties
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| Research Institution | Kyoto University |
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Principal Investigator |
TSUJI Hiroshi Kyoto University, 大学院・工学研究科, 助教 (20127103)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIKAWA Junzo 京都大学, 大学院・工学研究科, 教授 (80026278)
TAKAOKA Gikan 京都大学, 大学院・工学研究科, 教授 (90135525)
SATO Hiroko 京都大学, 大学院・工学研究科, 研究員 (00093245)
SOMMNI Piyanuch 京都大学, 大学院・工学研究科, 研究員 (40512968)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2008: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | 負イオン注入 / 表面改質 / 細胞親和性 / 間葉系幹細胞 / 神経回路網 / 細胞チップ / 負イオン注入処理 / パターン接着 / 親疎水性制御 / 自発的パターン配列接着 / 分化誘導 / 骨芽細胞 / パターン表面改質 / 生体適合性制御 / 石英ガラス / 人為的神経回路網 |
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| Research Abstract |
Mesenchymal stem cells (MSC) derived from bone-mallow of rat showed their self-aligned adhesion on polymer surfaces along the modified line-region in which carbon negative-ions were implanted through line-pattern mask for improving hydrophilicity. Besides, the adhered cells were differentiated into nerve cells and osteoblast cells with holding their adhesion pattern. By narrowing the width of the line pattern, the orientation of MSC cell body and nuclei were controlled to along with the long direction of the line pattern of the ion implantation. Cell sheet and string were formed by using C and O negative ion implantation into poly-lactic acid.
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Report
(4 results)
Research Products
(81 results)
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[Journal Article] Surface Modification of Silicone Rubber for Adhesion Patterning of Mesenchymal Stem Cells by Water Cluster Ion Beam2010
Author(s)
P.Sommani, G.Ichihashi, H.Ryuto, H.Tsuji, Y.Gotoh, G.H.Takaoka
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Journal Title
Ion Implantation 2010(Eds : J.Matsuo, M.Kase, T.Aoki, T.Seki, American Institute of Physics, 2010) Vol.IIT2010
Pages: 302-305
Related Report
Peer Reviewed
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[Journal Article] Negative ion implantation for pattering mesenchymal-stemcell adhesion on silicone rubber and differentiation into nerve cells with keeping their adhesion pattern2009
Author(s)
Hiroshi Tsuji, Piyanuch Sommani, Mitsutaka Hattori, Tetsuya Yamada, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa
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Journal Title
Surface and Coatings Technology, (In Press) H. Tsuji, et al., Surf. Coat. Technol., (2009), doi : 10.1016/j. surfcoat. 2009.02.076 (掲載決定)
Pages: 4-4
Related Report
Peer Reviewed
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[Presentation] Surface Modification of Silica Glass by CHF3 Plasma Treatment and Carbon Negative-Ion Implantation for Cell Pattern Adhesion2009
Author(s)
H.Tsuji, P.Sommani, Y.Hayashi, H.Kojima, H.Sato, Y.Gotoh, G.H.Takaoka, J.Ishikawa
Organizer
16th International Conference on Surface Modification of Materials by Ion Beam, SMMIB 2009, PC-06
Place of Presentation
Tokyo, Japan
Year and Date
2009-09-16
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