Control of Orientation and Shape of Adult Stem Cell on Base Surface by Negative-Ion Pattern Implantation and Induction of The Differentiation

• Project/Area Number: 20360018
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thin film/Surface and interfacial physical properties
• Research Institution: Kyoto University
• Principal Investigator: TSUJI Hiroshi Kyoto University, 大学院・工学研究科, 助教 (20127103)
• Co-Investigator(Kenkyū-buntansha): ISHIKAWA Junzo 京都大学, 大学院・工学研究科, 教授 (80026278) ; TAKAOKA Gikan 京都大学, 大学院・工学研究科, 教授 (90135525) ; SATO Hiroko 京都大学, 大学院・工学研究科, 研究員 (00093245) ; SOMMNI Piyanuch 京都大学, 大学院・工学研究科, 研究員 (40512968)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2008: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
• Keywords: 負イオン注入 / 表面改質 / 細胞親和性 / 間葉系幹細胞 / 神経回路網 / 細胞チップ / 負イオン注入処理 / パターン接着 / 親疎水性制御 / 自発的パターン配列接着 / 分化誘導 / 骨芽細胞 / パターン表面改質 / 生体適合性制御 / 石英ガラス / 人為的神経回路網

Research Abstract

Mesenchymal stem cells (MSC) derived from bone-mallow of rat showed their self-aligned adhesion on polymer surfaces along the modified line-region in which carbon negative-ions were implanted through line-pattern mask for improving hydrophilicity. Besides, the adhered cells were differentiated into nerve cells and osteoblast cells with holding their adhesion pattern. By narrowing the width of the line pattern, the orientation of MSC cell body and nuclei were controlled to along with the long direction of the line pattern of the ion implantation. Cell sheet and string were formed by using C and O negative ion implantation into poly-lactic acid.

Research Products

• [Journal Article] Surface Modification of Silica Glass by CHF3 Plasma Treatment and Carbon Negative-Ion Implantation for Cell Pattern Adhesion (2011)
  • Author(s): H.Tsuji, P.Sommani, Y.Hayashi, H.Kojima, H.Sato, Y.Gotoh, G.H.Takaoka, J.Ishikawa
  • Journal Title: Surface and Coatings Technology to be published
  • Peer Reviewed
• [Journal Article] Line-Width of Ion Beam-Modified Polystyrene by Negative Carbon Ions for Fine Adhesion Pattern of Mesenchymal Stem Cells (2011)
  • Author(s): P.Sommani, H.Tsuji, H.Kojima, H.Sato, Y.Gotoh, J.Ishikawa
  • Journal Title: Surface and Coatings Technology to be published
  • Peer Reviewed
• [Journal Article] Irradiation effect of carbon negative-ion implantation on polytetrafluoroethylene for controlling cell- adhesion property (2010)
  • Author(s): P.Sommani, H.Tsuji, H.Kojima, H.Sato, Y.Gotoh, J.Ishikawa
  • Journal Title: Nuclear Instruments and Methods in Physics Research B Vol.B268 Pages: 3231-3234
  • Peer Reviewed
• [Journal Article] Surface Modification of Silicone Rubber for Adhesion Patterning of Mesenchymal Stem Cells by Water Cluster Ion Beam (2010)
  • Author(s): P.Sommani, G.Ichihashi, H.Ryuto, H.Tsuji, Y.Gotoh, G.H.Takaoka
  • Journal Title: Ion Implantation 2010(Eds : J.Matsuo, M.Kase, T.Aoki, T.Seki, American Institute of Physics, 2010) Vol.IIT2010 Pages: 302-305
  • Peer Reviewed
• [Journal Article] Irradiation Effect of Carbon Negative-Ion Implantation on Polytetrafluoroethylene for Controlling Cell-Adhesion Property (2010)
  • Author(s): Piyanuch Sommani, Hiroshi Tsuji, Hiroyuki Kojima, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa
  • Journal Title: Nuclear Instruments and Methods in Physics Research B Volume: B268 Pages: 3231-3234
  • Peer Reviewed
• [Journal Article] Surface Modification of Silicone Rubber for Adhesion Patterning of Mesenchymal Stem Cells by Water Cluster Ion Beam (2010)
  • Author(s): Piyanuch Sommani, Gaku Ichihashi, Hiromichi Ryuto, Hiroshi Tsuji, Yasuhito Gotoh, Gikan H.Takaoka
  • Journal Title: Proceedings of the 18th International Conference on Ion Implantation Technology Volume: IIT2010 Pages: 302-305
  • Peer Reviewed
• [Journal Article] Surface Modification by Negative-Ion Implantation (2009)
  • Author(s): J.Ishikawa, H.Tsuji, Y.Gotoh
  • Journal Title: Surface and Coatings Technology Vol.203 Pages: 2351-2356
  • Peer Reviewed
• [Journal Article] Negative Ion Implantation for Pattering Mesenchymal Stem Cell Adhesion on Silicone Rubber and Differentiation into Nerve Cells with Preserving Their Adhesion Pattern (2009)
  • Author(s): H.Tsuji, P.Sommani, M.Hattori, T.Yamada, H.Sato, Y.Gotoh, J.Ishikawa
  • Journal Title: Surface and Coatings Technology Vol.203 Pages: 2562-2565
  • Peer Reviewed
• [Journal Article] Surface Modification by Negative-Ion Implantation (2009)
  • Author(s): Junzo Ishikawa, Hiroshi Tsuji, Yasuhito Gotoh
  • Journal Title: Surface and Coatings Technology 2351-2356 203 Pages: 2351-2356
  • Peer Reviewed
• [Journal Article] Negative Ion Implantation for Pattering Mesenchymal Stem Cell Adhesion on Silicone Rubber and Differentiation into Nerve Cells with Preserving Their Adhesion Pattern (2009)
  • Author(s): Hiroshi Tsuji, Piyanuch Sommani, Mitsutaka Hattori, Tetsuya Yamada, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa
  • Journal Title: Surface and Coatings Technology 2351-2356 203 Pages: 2562-2565
  • Peer Reviewed
• [Journal Article] Surface modification by negative-ion implantation (2009)
  • Author(s): Junzo Ishikawa, Hiroshi Tsuji, Yasuhito Gotoh
  • Journal Title: Surface and Coatings Technology, (In Press) J. Ishikawa, et al, Surf. Coat. Technol., (2009), doi : 10.1016/j. surfcoat. 2009.02.023 (掲載決定) Pages: 6-6
  • Peer Reviewed
• [Journal Article] Negative ion implantation for pattering mesenchymal-stemcell adhesion on silicone rubber and differentiation into nerve cells with keeping their adhesion pattern (2009)
  • Author(s): Hiroshi Tsuji, Piyanuch Sommani, Mitsutaka Hattori, Tetsuya Yamada, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa
  • Journal Title: Surface and Coatings Technology, (In Press) H. Tsuji, et al., Surf. Coat. Technol., (2009), doi : 10.1016/j. surfcoat. 2009.02.076 (掲載決定) Pages: 4-4
  • Peer Reviewed
• [Journal Article] Adhesion Patterning of Mesenchymal Stem Cells on Polystyrene Surface by Carbon Negative-Ion Implantation and Neuron Differentiation on the Position (2008)
  • Author(s): H.Tsuji, P.Sommani, M.Hattori, T.Yamada, H.Sato, Y.Gotoh, J.Ishikawa
  • Journal Title: Nuclear Instruments and Methods in Physics Research Vol.B266 Pages: 3067-3070
  • Peer Reviewed
• [Journal Article] Adhesion patterning of mesenchymal stem cells on polystyrene surface by carbon negative-ion implantation and neuron differentiation on the position (2008)
  • Author(s): Hiroshi Tsuji, Piyanuch Sommani, Mitsutaka Hattori, Tetsuya Yamada, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa
  • Journal Title: Nuclear Instruments and Methods in Physics Research B B266 Pages: 3067-3070
  • Peer Reviewed
• [Presentation] Ion Patterned Adhesion for Neuron Network Synthesis (2011)
  • Author(s): H.Tsuji
  • Organizer: European Material Research Society Spring Meeting E-MRS 2011, B4-1(Invited)
  • Place of Presentation: Nice, France
  • Year and Date: 2011-05-10
• [Presentation] Osteoblast Patterning on Silicone Rubber by using Mesenchymal Stem Cells and Carbon Negative-Ion (2010)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, Y.Gotoh, J.Ishikawa, G.H.Takaoka
  • Organizer: 21st International Academic Symposium of Materials Research Society of Japan, MRS-J2010, S-12-G
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2010-12-22
• [Presentation] Osteoblast Patterning on Silicone Rubber by using Mesenchymal Stem Cells and Carbon Negative-Ion (2010)
  • Author(s): Piyanuch Sommani, Hiroshi Tsuji, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa, Gikan Takaoka
  • Organizer: The International Academic Symposium of Materials Research Society of Japan, MRS-J 2010, S-12-G
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2010-12-21
• [Presentation] Pattern Modification of Polymer Surface by Negative Ion Beam for Neuron Network Formation (2010)
  • Author(s): Hiroshi Tsuji, Piyanuch Sommani, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa, Gikan H.Takaoka
  • Organizer: The 2nd International Symposium on Negative Ions, Beams and Sources, NIBS 2010, 0-42
  • Place of Presentation: Takayama, Japan
  • Year and Date: 2010-11-19
• [Presentation] Pattern modification of polymer surface by negative ion beam for neuron network formation (2010)
  • Author(s): H.Tsuji, P.Sommani, H.Sato, Y.Gotoh, J.Ishikawa, G.H.Takaoka
  • Organizer: International Negative Ion Beam Symposium, NIBS2010, O-42
  • Place of Presentation: Takayama, Japan.
  • Year and Date: 2010-11-18
• [Presentation] Negative-Ion Beam Modification of Silicone Rubber for Patterning Osteoblast from Differentiation of Mesenchymal Stem Cells (2010)
  • Author(s): Plyanuch Sommanil, Hiroshi Tsuji, Hiroko Sato, Yasuhito Gotoh, Gikan Takaoka
  • Organizer: 第51回真空に関する連合講演会、4P-020
  • Place of Presentation: 大阪大学、吹田
  • Year and Date: 2010-11-05
• [Presentation] Adhesion Control of Mesenchymal Stem Cells on Polymer and Glass for Artificial Neuron Network Formation by Negative-Ion Implantation (2010)
  • Author(s): Hiroshi Tsuji
  • Organizer: SI Special Lecture at Ecole Polytechnique 2010
  • Place of Presentation: Ecole Polytechnique, France
  • Year and Date: 2010-09-21
• [Presentation] Osteoblast Patterning from Mesenchymal Stem Cells on Silicone Rubber Patterned by Carbon Negative-Ion Implantation (2010)
  • Author(s): Piyanuch Sommani、辻博司、佐藤弘子、後藤康仁、石川順三、高岡義寛
  • Organizer: 2010年秋季 第71回応用物理学学術講演会,16p-ZL-2
  • Place of Presentation: 長崎大学、長崎
  • Year and Date: 2010-09-16
• [Presentation] Ion Beam Modification of Polymers for Self-Aligned Adhesion of Mesenchymal Stem Cells and Control of Nuclei Orientation (2010)
  • Author(s): H.Tsuji, P.Sommani, T.Yamada, H.Kojima, H.Sato, Y.Gotoh, J.Ishikawa
  • Organizer: 10th European Conference on Accelerators in Applied Research and Technology, ECAART 10, PI-14
  • Place of Presentation: Grifada, Greek.
  • Year and Date: 2010-09-13
• [Presentation] Ion Beam Modification of Polymers for Self-Aligned Adhesion of Mesenchymal Stem Cells and Control of Nuclei Orientation (2010)
  • Author(s): Hiroshi Tsuji, Piyanuch Sommani, Tetsuya Yamada, Hiroyuki Kojima, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa
  • Organizer: The 10th European Conference on Accelerators in Applied Research and Technology, ECAART 10, PI-14
  • Place of Presentation: Grifada, Greece
  • Year and Date: 2010-09-13
• [Presentation] Negative-ion beam degradation of proteins for adhesion patterning of brain neuronal cells (2010)
  • Author(s): H.Tsuji, P.Sommani, H.Sato, Y.Gotoh, J.Ishikawa, G.H.Takaoka
  • Organizer: 17th International Conference on Ion Beam Modification of Materials, IBMM2010, #336
  • Place of Presentation: Montreal, Canada.
  • Year and Date: 2010-08-24
• [Presentation] Negativeion beam modified polymeric surfaces for controls of individual cell arrangement and cell-nucleus orientation (2010)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, T.Yamada, H.Kojima, Y.Gotoh, J.Ishikawa
  • Organizer: 17th International Conference on Ion Beam Modification of Materials, IBMM2010, #455
  • Place of Presentation: Montreal, Canada.
  • Year and Date: 2010-08-24
• [Presentation] Negative-Ion Beam Degradation of Proteins for Adhesion Patterning of Brain Neuronal Cells (2010)
  • Author(s): Hiroshi Tsuji, Piyanuch Sommni, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa, Gikan H.Takaoka
  • Organizer: The 17th International Conference on Ion Beam Modification of Materials, IBMM2010, #336
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2010-08-24
• [Presentation] Negative-Ion Beam Modified Polymeric Surfaces for Controls of Individual Cell Arrangement and Cell-Nucleus Orientation (2010)
  • Author(s): Piyanuch Sommani, Hiroshi Tsuji, Hiroko Sato, Tetsuya Yamada, Hiroyuki Kojima, Yasuhito Gotoh, Junzo Ishikawa
  • Organizer: The 17th International Conference on Ion Beam Modification of Materials, IBMM2010, #455
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2010-08-24
• [Presentation] Surface Modification of Silicone Rubber for Adhesion Patterning of Mesenchymal Stem Cells by Water Cluster Ion Beam (2010)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, Y.Gotoh, J.Ishikawa, G.H.Takaoka
  • Organizer: 18th International Conference on Ion Implantation Technology, IIT2010, P2-58
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2010-07-07
• [Presentation] Surface Modification of Silicone Rubber for Adhesion Patterning of Mesenchymal Stem Cells by Water Cluster Ion Beam (2010)
  • Author(s): Piyanuch Sommani, Hiroshi Tsuji, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa, Gikan H.Takaoka
  • Organizer: The 18th International Conference on Ion Implantation Technology, IIT2010, P2-58
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2010-06-09
• [Presentation] Adhesion Patterning of Mesenchymal Stem Cells on Silicone Rubber by Irradiation of Water Cluster Ions (2010)
  • Author(s): P.Sommani, G.Ichihashi, H.Ryuto, H.Tsuji, Y.Gotoh, G.H.Takaoka
  • Organizer: 2010年春季 第57回応用物理学関係連合講演会
  • Place of Presentation: 東海大学、平塚
  • Year and Date: 2010-03-20
• [Presentation] Carbon Negative-Ion Implantation for Positive- Pattern of Avidin on Polystyrene (2009)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, Y.Gotoh, J.Ishikawa, G.H.Takaoka
  • Organizer: 20th International Academic Symposium of MRS-J2009, R-17-G
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2009-12-09
• [Presentation] Carbon Negative-Ion Implantation for Positive-Pattern of Avidin on Polystyrene (2009)
  • Author(s): Piyanuch Sommani, Hiroshi Tsuji, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa, Gikan Takaoka
  • Organizer: The International Academic Symposium of Material Research Society of Japan, MRS-J2009
  • Place of Presentation: 横浜開港記念会館、横浜
  • Year and Date: 2009-12-09
• [Presentation] Cell Adhesion Pattering on Polystyrene by Using Avidin Coating and Carbon Negative-Ion Implantation (2009)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, Y.Gotoh, J.Ishikawa, G.H.Takaoka
  • Organizer: 20th International Academic Symposium of MRS-J2009, R-P05-G
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2009-12-08
• [Presentation] Degradation of Proteins for Neuronal-Cell-Adhesion Pattering by Carbon Negative-Ion Implantation (2009)
  • Author(s): H.Tsuji, P.Sommani, H.Sato, Y.Gotoh, J.Ishikawa, G.H.Takaoka
  • Organizer: 20th International Academic Symposium of MRS-J2009, R-P06
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2009-12-08
• [Presentation] Degradation of Proteins for Neuronal-Cell-Adhesion Pattering by Carbon Negative-Ion Implantation (2009)
  • Author(s): Hiroshi Tsuji, Piyanuch Sommani, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa, Gikan Takaoka
  • Organizer: The International Academic Symposium of Material Research Society of Japan, MRS-J2009
  • Place of Presentation: 横浜開港記念会館、横浜
  • Year and Date: 2009-12-08
• [Presentation] Cell Adhesion Pattering on Polystyrene by Using Avidin Coating and Carbon Negative-Ion Implantation (2009)
  • Author(s): Piyanuch Sommani, Hiroshi Tsuji, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa, Gikan Takaoka
  • Organizer: The International Academic Symposium of Material Research Society of Japan, MRS-J2009
  • Place of Presentation: 横浜開港記念会館、横浜
  • Year and Date: 2009-12-08
• [Presentation] 負イオンビームによる材料表面の細胞接着性制御とその応用-幹細胞のパターン接着と神経回路網形成- (2009)
  • Author(s): 辻博司, Piyanuch Sommani, 後藤康仁, 石川順三
  • Organizer: 日本学術振興会 荷電粒子ビームの工業への応用学振132委員会 第188回研究会
  • Place of Presentation: 弘済会館、東京
  • Year and Date: 2009-11-27
• [Presentation] 炭素負イオン注入によるCHF3プラズマ処理石英ガラス上での間葉系幹細胞のパターン接着 (2009)
  • Author(s): 辻博司, Piyanuch Sommani, 佐藤弘子, 後藤康仁, 高岡義寛, 石川順三
  • Organizer: 第50回真空に関する連合講演会
  • Place of Presentation: 学習院大学、東京
  • Year and Date: 2009-11-05
• [Presentation] Fine Adhesion Patterning and Aligned Nuclei Orientation of Mesenchymal Stem Cell on Narrow Line-Width of Silicone Rubber Implanted by Carbon Negative Ions (2009)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, Y.Gotoh, G.Takaoka, J.Ishikawa
  • Organizer: 第50回真空に関する連合講演会
  • Place of Presentation: 学習院大学、東京
  • Year and Date: 2009-11-05
• [Presentation] Surface Modification of Silica Glass by CHF3 Plasma Treatment and Carbon Negative-Ion Implantation for Cell Pattern Adhesion (2009)
  • Author(s): H.Tsuji, P.Sommani, Y.Hayashi, H.Kojima, H.Sato, Y.Gotoh, G.H.Takaoka, J.Ishikawa
  • Organizer: 16th International Conference on Surface Modification of Materials by Ion Beam, SMMIB 2009, PC-06
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2009-09-16
• [Presentation] Line-Width of Ion Beam-Modified Polystyrene by Negative Carbon Ions for Fine Adhesion Pattern of Mesenchymal Stem Cells (2009)
  • Author(s): P.Sommani, H.Tsuji, H.Kojima, H.Sato, Y.Gotoh, J.Ishikawa
  • Organizer: 16th International Conference on Surface Modification of Materials by Ion Beam, SMMIB2009, PC-13
  • Place of Presentation: Tokyo, Japan.
  • Year and Date: 2009-09-16
• [Presentation] Surface Modification of Silica Glass by CHF3 Plasma Treatment and Carbon Negative-Ion Implantation for Cell Pattern Adhesion (2009)
  • Author(s): Hiroshi Tsuji, Piyanuch Sommani, Yuichiro Hayashi, Hiroyuki Kojima, Hiroko Sato, Yasuhito Gotoh, Gikan Takaoka, Junzo Ishikawa
  • Organizer: The 16th International Conference on Surface Modification of Materials by Ion Beam, SMMIB 2009
  • Place of Presentation: 産業技術総合研究所、東京
  • Year and Date: 2009-09-16
• [Presentation] Line-Width of Ion Beam-Modified Polystyrene by Negative Carbon Ions for Fine Adhesion Pattern of Mesenchymal Stem Cells (2009)
  • Author(s): Piyanuch Sommani, Hiroshi Tsuji, Hiroyuki Kojima, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa
  • Organizer: The 16th International Conference on Surface Modification of Materials by Ion Beam, SMMIB 2009
  • Place of Presentation: 産業技術総合研究所、東京
  • Year and Date: 2009-09-16
• [Presentation] 炭素負イオン狭パターン注入処理による間葉系幹細胞の細胞核配列制御 (2009)
  • Author(s): 辻博司, Piyanuch Sommani, 小嶌裕之, 佐藤弘子, 後藤康仁, 石川順三
  • Organizer: 2009年秋季 第70回応用物理学学術講演会
  • Place of Presentation: 富山大学、富山
  • Year and Date: 2009-09-08
• [Presentation] 炭素負イオン注入によるポリテトラフルオロエチレン上の間葉系幹細胞の接着制御 (2009)
  • Author(s): Piyanuch Sommani, 辻博司, 佐藤弘子, 後藤康仁, 石川順三, 高岡義寛
  • Organizer: 2009年秋季 第70回応用物理学学術講演会
  • Place of Presentation: 富山大学、富山
  • Year and Date: 2009-09-08
• [Presentation] Carbon Negative-Ion Implantation on Silicone Rubber with Narrower Line Width for Pattern Adhesion of Mesenchymal Stem Cells and Control of Nuclei Orientation (2009)
  • Author(s): H.Tsuji, P.Sommani, T.Yamada, H.Kojima, H.Sato, Y.Gotoh, J.Ishikawa
  • Organizer: 15th International Conference on Radiation Effects in Insulators, REI-15, C20
  • Place of Presentation: Padova, Italy
  • Year and Date: 2009-09-03
• [Presentation] Radiation Effect of Carbon Negative-Ion Implantation on Polytetrafluoroethylene for Controlling Cell-Adhesion Property (2009)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, T.Yamada, H.Kojima, Y.Gotoh, J.Ishikawa
  • Organizer: 15th International Conference on Radiation Effects in Insulators, REI-15, C22
  • Place of Presentation: Padova, Italy.
  • Year and Date: 2009-09-03
• [Presentation] Carbon Negative-Ion Implantation on Silicone Rubber with Narrower Line Width for Pattern Adhesion of Mesenchymal Stem Cells and Control of Nuclei Orientation (2009)
  • Author(s): Hiroshi Tsuji, Piyanuch Sommani, Tetsuya Yamada, Hiroyuki Kojima, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa
  • Organizer: The 15th International Conference on Radiation Effects in Insulators, REI-15
  • Place of Presentation: Padova, Italy
  • Year and Date: 2009-09-03
• [Presentation] Radiation Effect of Carbon Negative-Ion Implantation on Polytetrafluoroethylene for Controlling Cell-Adhesion Property (2009)
  • Author(s): Piyanuch Sommani, Hiroshi Tsuji, Hiroko Sato, Tetsuya Yamada, Hiroyuki Kojima, Yasuhito Gotoh, Junzo Ishikawa
  • Organizer: The 15th International Conference on Radiation Effects in Insulators, REI-15
  • Place of Presentation: Padova, Italy
  • Year and Date: 2009-09-03
• [Presentation] Adhesion Patterning of Mesenchymal Stem Cells on Silicone Rubber by Carbon Negative-Ion Pattern Implantation for Artificial Nerve-Cell Network Formation (Invited) (2009)
  • Author(s): 辻博司
  • Organizer: IEF Special Seminar of University of Paris XI (Sud)
  • Place of Presentation: Univ. of Paris XI, Orsay, France
  • Year and Date: 2009-08-28
• [Presentation] 炭素負イオンパターン注入処理による高分子材料上での間葉系幹細胞のパターン配列接着制御と神経細胞への分化誘導 (2009)
  • Author(s): 辻博司
  • Organizer: 平成21年度核融合科学研究所共同研究研究会「負イオン生成および負イオンビーム加速とその応用」
  • Place of Presentation: 核融合科学研究所、土岐
  • Year and Date: 2009-08-19
• [Presentation] Mesenchymal Stem Cell Patterning and Its Differentiation into Neuron on Silicone Rubber Modified by Carbon Negative-IoImplantation (2009)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, T.Yamada, H.Yamada, Y.Gotoh, J.Ishikawa
  • Organizer: 2nd Asian Biomaterials Congress 2009 in conjunction with IUMRS-ICA2009, ABMC-1-07P
  • Place of Presentation: Singapore
  • Year and Date: 2009-07-26
• [Presentation] Adhesion Patterning of Mesenchymal Stem Cells on Silicone Rubber by Carbon Negative-Ion Pattern Implantation for Artificial Nerve-Cell Network Formation (Invited) (2009)
  • Author(s): 辻博司
  • Organizer: CIM special lecture, at Alabama A&M University
  • Place of Presentation: Alabama A&M Univ. Normal, USA
  • Year and Date: 2009-07-19
• [Presentation] Adhesion Patterning of Mesenchymal Stem Cells on Silicone Rubber by Carbon Negative-Ion Implantation for Artificial Nerve-Cell Network Formation(invited talk) (2009)
  • Author(s): H.Tsuji
  • Organizer: Huntsville Ion Beam Institute, HIBI2009
  • Place of Presentation: Huntsville, AL USA.
  • Year and Date: 2009-07-14
• [Presentation] Adhesion Patterning of Mesenchymal Stem Cells on Silicone Rubber by Carbon Negative-Ion Implantation for Artificial Nerve-Cell Network Formation (Invited) (2009)
  • Author(s): 辻博司
  • Organizer: 2009 Huntsville Ion Beam Institute, HIBI2009
  • Place of Presentation: Huntsville, USA
  • Year and Date: 2009-07-14
• [Presentation] Adhesion Patterning of Mesenchymal Stem Cells on Polymeric Materials by Carbon Negative-Ion Implantation through a Mask and Their Differentiation into Neurons with Keeping Cell Alignment (Invited) (2009)
  • Author(s): 辻博司
  • Organizer: Special Seminar at Chiang Mai University, Plasma and Beam Physics Research Facility
  • Place of Presentation: Chiang Mai, Thailand
  • Year and Date: 2009-06-29
• [Presentation] Minimum Line-Width on Silicone Rubber Modified by Carbon Negative-Ion Implantation for Self-Aligned Adhesion of Mesenchymal Stem Cells and Orientation Control of Nucleus (2009)
  • Author(s): Hiroshi Tsuji, Piyanuch Sommani, Hiroyuki Kojima, Yuichiro Hayashi, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa, Gikan Takaoka
  • Organizer: The 2nd Asian Biomaterials Congress 2009 in conjunction with IUMRS-ICA2009
  • Place of Presentation: Singapore
  • Year and Date: 2009-06-27
• [Presentation] Mesenchymal Stem Cell Patterning and Its Differentiation into Neuron on Silicone Rubber Modified by Carbon Negative-Io Implantation (2009)
  • Author(s): Piyanuch Sommani, Hiroshi Tsuji, Hiroko Sato, Tetsuya Yamada, Hiroyuki Yamada, Yasuhito Gotoh, Junzo Ishikawa
  • Organizer: The 2nd Asian Biomaterials Congress 2009 in conjunction with IUMRS-ICA2009
  • Place of Presentation: Singapore
  • Year and Date: 2009-06-27
• [Presentation] Minimum Line-Width on Silicone Rubber Modified by Carbon Negative-Ion Implantation for Self-Aligned Adhesion of Mesenchymal Stem Cells and Orientation Control of Nucleus (2009)
  • Author(s): H.Tsuji, P.Sommani, H.Kojima, Y.Hayashi, H.Sato, Y.Gotoh, J.Ishikawa, G.H.Takaoka
  • Organizer: 2nd Asian Biomaterials Congress 2009 in conjunction with IUMRS-ICA2009, ABMC-1-06P
  • Place of Presentation: Singapore.
  • Year and Date: 2009-06-26
• [Presentation] 表面フッ化石英ガラス表面への炭素負イオン注入処理による細胞のパターン接着 (2009)
  • Author(s): 辻博司, ソムマニ・ピヤヌット, 小嶌裕之, 林雄一郎, 佐藤弘子, 後藤康仁, 石川順三
  • Organizer: 2009年春季第56回応用物理学関係連合講演会
  • Place of Presentation: 筑波大学、つくば
  • Year and Date: 2009-03-30
• [Presentation] Improvement of Physical Surface Property of Polytetrafluoroethylene by Carbon Negative-Ion Implantation for Cell Adhesion (2009)
  • Author(s): P. Sommani, H. Tsuji, Y. Hayahi, H. Kojima, H. Sato, Y. Gotoh, J. Ishikawa
  • Organizer: 2009年春季第56回応用物理学関係連合講演会
  • Place of Presentation: 筑波大学、つくば
  • Year and Date: 2009-03-30
• [Presentation] Adhesion Pattering of Mesenchymal Stem Cells on Polymeric Surfaces by Carbon Negative-Ion Implantation through a Mask and Their Differentiation into Neurons with Keeping Cell Adhesion (2009)
  • Author(s): Hiroshi Tsuji
  • Organizer: Special Seminar of Department of Chemical Science, University of Catania
  • Place of Presentation: Catania, Italy
  • Year and Date: 2009-03-09
• [Presentation] Nuclei Orientation of Mesenchymal Stem Cells on Narrow Regions of Silicone Rubber Modified by Carbon Negative-Ion Implantation (2008)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, H.Kojima, Y.Gotoh, J.Ishikawa
  • Organizer: IUMRS International Conference in Asia 2008, IUMRS-ICA2008, MO-3
  • Place of Presentation: Nagoya, Japan.
  • Year and Date: 2008-12-10
• [Presentation] Comparison of Implantation Conditions of Carbon Negative Ions for Pattern Adhesion of Mesenchymal Stem Cells on Polymeric Surfaces (2008)
  • Author(s): H Tsuji, P Sommani, T Yamada, H Kojima, H Sato, Y Gotoh, o Ishikawa
  • Organizer: IUMRS International Conference in Asia 2008, IUMRS-ICA2008, MP-3
  • Place of Presentation: Nagoya, Japan.
  • Year and Date: 2008-12-10
• [Presentation] Nuclei Orientation of Mesenchymal Stem Cells on Narrow Regions of Silicone Rubber Modified by Carbon Negative-Ion Implantation (2008)
  • Author(s): Piyanuch Sommani, Hiroshi Tsuji, Hiroko Sato, Hiroyuki Kojima, Yasuhito Gotoh, Junzo Ishikawa
  • Organizer: The IUMRS International Conference in Asia 2008 (IUMRS-ICA2008)
  • Place of Presentation: 名古屋国際会議場、名古屋
  • Year and Date: 2008-12-10
• [Presentation] Comparison of Implantation Conditions of Carbon Negative Ions for Pattern Adhesion of Mesenchymal Stem Cells on Polymeric Surfaces (2008)
  • Author(s): Hiroshi Tsuji, Piyanuch Sommani, Tetsuya Yamada, Hiroyuki Kojima, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa
  • Organizer: The IUMRS International Conference in Asia 2008 (IUMRS-ICA2008)
  • Place of Presentation: 名古屋国際会議場、名古屋
  • Year and Date: 2008-12-10
• [Presentation] Effect of Decreasing Line-Width in Carbon Negative-Ion Pattern-Implantation into Polystyrene Surface upon Adhesion of Mesenchymal Stem Cells (2008)
  • Author(s): H.Tsuji, P.Sommani, H.Kojima, H.Sato, Y.Gotoh, J.Ishikawa
  • Organizer: 4th Vacuum and Surface Sciences Conference of Asia and Australia, VASSCAA-4, 28P100
  • Place of Presentation: Matsue, Japan.
  • Year and Date: 2008-10-28
• [Presentation] C-implantation for Avidin Patterning on Polystyrene (2008)
  • Author(s): P.Sommani, H.Tsuji, H.Kojima, H.Sato, Y.Gotoh, J.Ishikawa
  • Organizer: The 4th Vacuum and Surface Sciences Conference of Asia and Australia, VASSCAA-4, 28P099
  • Place of Presentation: Matsue, Japan.
  • Year and Date: 2008-10-28
• [Presentation] Effect of Decreasing Line-Width in Carbon Negative-Ion Pattern-Implantation into Polystyrene Surface upon Adhesion of Mesenchymal Stem Cells (2008)
  • Author(s): Hiroshi Tsuji, Piyanuch Sommani, Hiroyuki Kojima, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa
  • Organizer: 4th Vacuum and Surface Sciences Conference of Asia and Australia (VASSCAA-4)
  • Place of Presentation: くにびきメッセ、松江
  • Year and Date: 2008-10-28
• [Presentation] C^--implantation for Avidin Patterning on Polystyrene (2008)
  • Author(s): Piyanuch Sommani, Hiroshi Tsuji, Hiroyuki Kojima, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa
  • Organizer: 4th Vacuum and Surface Sciences Conference of Asia and Australia (VASSCAA-4)
  • Place of Presentation: くにびきメッセ、松江
  • Year and Date: 2008-10-28
• [Presentation] ガラス基板上での細胞接着制御を目的としたCHF3プラズマによる前処理 (2008)
  • Author(s): 小嶌裕之, 辻博司, ソムマニ・ピヤヌット, 佐藤弘子, 後藤康仁, 石川順三
  • Organizer: 第49回真空に関する連合講演会
  • Place of Presentation: くにびきメッセ、松江
  • Year and Date: 2008-10-28
• [Presentation] Modified Minimum Line-Width in Carbon Negative-Ion Implantation for Self-Aligned Adhesion of Mesenchymal Stem Cells on Silicone Rubber and Orientation Control of Nucleus (2008)
  • Author(s): H.Tsuji, P.Sommani, T.Yamada, H.Kojima, H.Sato, Y.Gotoh, J.Ishikawa
  • Organizer: 16th International Conference on Ion Beam Modification of Materials, IBMM08, PB14-03
  • Place of Presentation: Dresden, Germany.
  • Year and Date: 2008-09-03
• [Presentation] Negative-Ion Beam Modified Silicone Rubber for Patterning Adhesions of Mesenchymal Stem Cell and Its Differentiated Neurons (2008)
  • Author(s): P.Sommani, H.Tsuji, H.Sato, T.Yamada, H.Kojima, Y.Gotoh, J.Ishikawa
  • Organizer: 16th International Conference on Ion Beam Modification of Materials, IBMM08, PB14-04
  • Place of Presentation: Dresden, Germany.
  • Year and Date: 2008-09-03
• [Presentation] Modified Minimum Line-Width in Carbon Negative-Ion Implantation for Self-Aligned Adhesion of Mesenchymal Stem Cells on Silicone Rubber and Orientation Control of Nucleus (2008)
  • Author(s): Hiroshi Tsuji, Piyanuch Sommani, Tetsuya Yamada, Hiroyuki Kojima, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa
  • Organizer: 16th International Conference on Ion Beam Modification of Materials (IBMM 08)
  • Place of Presentation: Dresden, Germany
  • Year and Date: 2008-09-03
• [Presentation] Negative-Ion Beam Modified Silicone Rubber for Patterning Adhesions of Mesenchymal Stem Cell and Its Differentiated Neurons (2008)
  • Author(s): Piyanuch Sommani, Hiroshi Tsuji, Hiroko Sato, Tetsuya Yamada, Hiroyuki Kojima, Yasuhito Gotoh, Junzo Ishikawa
  • Organizer: 16th International Conference on Ion Beam Modification of Materials (IBMM 08)
  • Place of Presentation: Dresden, Germany
  • Year and Date: 2008-09-03
• [Presentation] ガラス基板上での細胞接着制御を目的としたCHF3プラズマによる前処理 (2008)
  • Author(s): 小嶌裕之, 辻博司, ソムマニ・ピヤヌット, 佐藤弘子, 後藤康仁, 石川順三
  • Organizer: 2008年秋季第69回応用物理学学術講演会
  • Place of Presentation: 中部大学、春日井
  • Year and Date: 2008-09-02
• [Presentation] Principal Concepts in Develop- ment of Ion Sources in Kyoto University (invited) (2008)
  • Author(s): J.Ishikawa
  • Organizer: 9th International Conference on Applications of Nuclear Techniques, CRETE08
  • Place of Presentation: Crete, Greek.
  • Year and Date: 2008-06-12
• [Presentation] Neuron Arrangement on Silicone Rubber by Using Differentiation from Mesenchymal Stem Cells and Carbon Negative-Ion Implantation (2008)
  • Author(s): H.Tsuji, P.Sommani, M.Hattori, T.Yamada, H.Sato, Y.Gotoh, J.Ishikawa
  • Organizer: 9th International Conference on Applications of Nuclear Techniques, CRETE08
  • Place of Presentation: Crete, Greek.
  • Year and Date: 2008-06-12
• [Presentation] Neuron Arrangement on Silicone Rubber by Using Differentiation from Mesenchymal Stem Cells and Carbon Negative-Ion Implantation (2008)
  • Author(s): Hiroshi Tsuji, Piyanuch Sommani, Mitsutaka Hattori, Tetsuya Yamada, Hiroko Sato, Yasuhito Gotoh, Junzo Ishikawa
  • Organizer: 9th International Conference on Applications of Nuclear Techniques (CRETE08)
  • Place of Presentation: Crete, Greece
  • Year and Date: 2008-06-12
• [Presentation] Line-Width Limitation of Ion Beam-Modified Silicone Rubber by Negative Ions for Adhesion of Mesenchymal Stem Cells (2008)
  • Author(s): Piyanuch Sommani, Hiroshi Tsuji, Hiroko Sato, Tetsuya Yamada, Hiroyuki Yamada, Yasuhito Gotoh, Junzo Ishikawa
  • Organizer: 9th International Conference on Applications of Nuclear Techniques (CRETE08)
  • Place of Presentation: Crete, Greece
  • Year and Date: 2008-06-12