Analysis of molecular recognition in liquid by pulse-modulated attractive force microscopy

• Project/Area Number: 20360020
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thin film/Surface and interfacial physical properties
• Research Institution: Osaka University
• Principal Investigator: MATSUMOTO Takuya 大阪大学, 理学研究科, 教授 (50229556)
• Research Collaborator: TAKAGI Akihiko 大阪大学, 免疫学フロンティア研究センター・企画室, 特任准教授(常勤) ; MAEDA Yasushi 産業技術総合研究所, 関西センター・ユビキタスエネルギー研究部門・ナノ材料科学研究グループ, 研究員
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2009: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: 走査プローブ / 分子認識 / 分子電極 / パルス変調 / 水溶液 / バルス変調 / 分子分極

Research Abstract

We revealed that the surface electric-field modulation from the backward electrode of insulating substrate was effective for surface molecular adsorbates. We demonstrated that electrostatic force microscopy gives characteristic contrast inversion between DNA and transcription complex images reflecting the difference of electric polarizability of these molecules. These findings indicate that the electrostatic properties of individual biological molecules can be imaged on an insulator substrate suggesting the basis of pulsed-modulated attractive force microscopy.

Research Products

• [Journal Article] Imaging of Transverse Electron Transfer through a DNA Molecule by Simultaneous Scanning Tunneling and Frequency-Modulation Atomic Force Microscopy (2012)
  • Author(s): Y.Maeda, T.Matsumoto, T.Kawai
  • Journal Title: ACS Nano Volume: 5[4]巻 Issue: 4 Pages: 3141-3145
  • DOI: 10.1021/nn200291f
  • Peer Reviewed
• [Journal Article] Nanoparticle Arrangement by DNA-programmed Self-assembly for Catalyst Applications (2010)
  • Author(s): Y. Maeda, T. Akita, M. Date, A. Takagi, T. Matsumoto, T. Fujitani, M. Kohyama
  • Journal Title: J. Appl. Phys. Volume: 108[ 9]
  • Peer Reviewed
• [Journal Article] Nanoparticle Arrangement by DNA-programmed Self-assembly for Catalyst Applications (2010)
  • Author(s): Y.Maeda, T.Akita, M.Date, A.Takagi, T.Matsumoto, T.Fujitani, M.Kohyama
  • Journal Title: J.Appl.Phys. 108[9]
  • Peer Reviewed
• [Journal Article] AFM Nanopatterning of Transition Metal Oxide thin Films (2010)
  • Author(s): L.Pellegrino, I Pallecchi, E.Gellingeri, G.Canu, A.S.Siri, D.Marre, Y.Yanagisawa, M.Ishikawa, T.Matsumoto, Hide.Tanaka, T.Kawai
  • Journal Title: J.Nanosci.Nanotech Volume: 10[7] Pages: 4471-4476
  • Peer Reviewed
• [Journal Article] Nano-scale resistivity reduction in single grain of lead phthalocyanine (2010)
  • Author(s): S.Tabuchi, Y.Otsuka, M.Kanai, H.Tabata, T.Matsumoto, T.Kawai
  • Journal Title: Organic Electronics Volume: 11[5] Pages: 916-924
  • Peer Reviewed
• [Journal Article] Electrostatic Force Spectroscopy on InsulatingSurfaces : the Effect of Capacitive Interaction (2009)
  • Author(s): A. Takagi, F. Yamada, T. Matsumoto, T. Kawai
  • Journal Title: Nanotechnology Volume: 20[ 36]
  • Peer Reviewed
• [Journal Article] Electrostatic ForceMicroscopy : Imaging DNA and Protein Polarizations One by One (2009)
  • Author(s): E. Mikamo-Satoh, F. Yamada, A. Takagi, T. Matsumoto, T. Kawai
  • Journal Title: Nanotechnology Volume: 20[ 14]
  • Peer Reviewed
• [Journal Article] Electrostatic Force Spectroscopy on Insulating Surfaces : the Effect of Capacitive Interaction (2009)
  • Author(s): A.Takagi, F.Yamada, T.Matsumoto, T.Kawai
  • Journal Title: Nanotechnology 20[36]
  • Peer Reviewed
• [Journal Article] Electrostatic Force Microscopy : Imaging DNA and Protein Polarizations One by One (2009)
  • Author(s): E.Mikamo-Satoh, F.Yamada, A.Takagi, T.Matsumoto, T.Kawai
  • Journal Title: Nanotechnology 20[14]
  • Peer Reviewed
• [Journal Article] Profiling of Gene-dependent Translational Progress in Cell-free Protein Synthesis by Real-space Imaging (2009)
  • Author(s): Eriko Mikamo-Satoh
  • Journal Title: Anal.Biochem. 394[2] Pages: 275-280
  • Peer Reviewed
• [Journal Article] Electrostatic Force Spectroscopy on Insulating Surfaces : the Effect of Capaciticve Interaction (2009)
  • Author(s): Akihiko Takagi
  • Journal Title: Nanotechnology 20[36]
  • Peer Reviewed
• [Journal Article] Electrostatic Force Microscopy : Imaging DNA and Protein Polarizations One by One (2009)
  • Author(s): Eriko Mikamo-Satoh
  • Journal Title: Nanotechnology 20[14]
  • Peer Reviewed
• [Journal Article] 走査プローブ顕微鏡を用いた時間分解静電気力検出法の開発 (2008)
  • Author(s): 松本卓也
  • Journal Title: 顕微鏡 43[2] Pages: 149-151
  • NAID: 130007791682
  • Peer Reviewed
• [Presentation] Imaging of Transverse Electron Transfer through DNA Molecule by Simultaneous Scanning Tunneling and Frequency-Modulation Microscopy (2011)
  • Author(s): Yasushi. Maeda
  • Organizer: 19th International Colloquium on Scanning Probe Microscopy (ICSPM19)
  • Place of Presentation: Toyako Manseikaku, (Hokkaido, Japan)
  • Year and Date: 2011-12-20
• [Presentation] バイオ分子の分極画像 (2011)
  • Author(s): 松本卓也
  • Organizer: 第8回パイオオブティクス研究会・理研シンポジウム「蛍光相関分光と情報伝達(8)」合同シンポジウム
  • Place of Presentation: 北里大学相模原キャンパス(相模原市)
  • Year and Date: 2011-12-17
• [Presentation] バイオ分子の分極画像 (2011)
  • Author(s): 松本卓也
  • Organizer: 第8回バイオオプティクス研究会・理研シンポジウム「蛍光相関分光と情報伝達(8)」合同シンポジウム
  • Place of Presentation: 北里大学相模原キャンパス(神奈川県・相模原市)
  • Year and Date: 2011-12-17
• [Presentation] STM/NC-AFM同時測定によるDNAの電気伝導解析 (2011)
  • Author(s): 松本卓也
  • Organizer: 2011年度関西薄膜表面物理セミナー
  • Place of Presentation: グリーンビレッジ交野(大阪府・交野市)
  • Year and Date: 2011-11-25
• [Presentation] TomogiKawai, Imaging of Transverse Electron Transferthrough DNA Molecule by Simultaneous Scanning Tunneling and Frequency-Modulation Microscopy (2011)
  • Author(s): Yasushi. Maeda、Takuya Matsumoto
  • Organizer: 19th International Colloquium onScanning Probe Microscopy(ICSPM19)
  • Place of Presentation: Toyako Manseikaku,(Hokkaido, Japan)
  • Year and Date: 2011-10-20
• [Presentation] Control of Network Formation of DNA Origami and λ-DNA : Emergent One-pot Processes Utilizing Binary Solvent (2010)
  • Author(s): Takuya Matsumoto
  • Organizer: 5th International Meeting on Molecular Electronics [ElecMol'10]
  • Place of Presentation: Maison MINATEC (Grenoble,France)
  • Year and Date: 2010-12-09
• [Presentation] ソフトナノマテリアルの電流経路イメージング(招待講演) (2010)
  • Author(s): *松本卓也
  • Organizer: 第57回応用物理学関係連合講演会
  • Place of Presentation: 東海大学湘南キャンパス(神奈川県・平塚市)
  • Year and Date: 2010-03-19
• [Presentation] 分子スケールエレクトロニクス-単分子デバイスから単分子集積デバイスへ- (2010)
  • Author(s): 松本卓也
  • Organizer: 大阪大学有機エレクトロニクス研究会春の学校「単一分子エレクトロニクスの要素技術と基礎理論」
  • Place of Presentation: 国際高等研究所(京都府木津川市)(招待講演)
  • Year and Date: 2010-02-26
• [Presentation] Nanotester : Imaging the Current Pathway of Soft Material (招待講演) (2010)
  • Author(s): Takuya Matsumoto
  • Organizer: Germany/Japan, 1^<st> Workshop on "Nanoanalytics"
  • Place of Presentation: Osaka University Nakanoshima Center (Osaka, Japen)
  • Year and Date: 2010-02-15
• [Presentation] Electrostatic Force Microscopy/Spectroscopy on Insulating Substrates : Effect of Capacitive Interactions in Vacuum and Water (招待講演) (2009)
  • Author(s): *Takuya. Matsumoto
  • Organizer: 17^<th> International Colloquium on Scanning Probe Microscopy (ICSPM17)
  • Place of Presentation: Atagawa Heights (Sizuoka, Japan)
  • Year and Date: 2009-12-11
• [Presentation] 周波数シフト法を用いた液中引力計測 (2009)
  • Author(s): 河野正人
  • Organizer: 第29回表面科学学術講演会
  • Place of Presentation: タワーホール船堀(東京)
  • Year and Date: 2009-10-29
• [Presentation] Electrostatic Force Microscopy/ Spectroscopy on Insulating Substrates : Effect of Capacitive Interactions inVacuum and Water (2009)
  • Author(s): Takuya. Matsumoto
  • Organizer: 17^<th> International Colloquium on Scanning Probe Microscopy(ICSPM17)(招待講演)
  • Place of Presentation: Atagawa Heights(Sizuoka, Japan)
  • Year and Date: 2009-10-11
• [Presentation] 絶縁体表面におけるバイオ分子の引力イメージング (2009)
  • Author(s): 松本卓也
  • Organizer: 第70回応用物理学学術講演会
  • Place of Presentation: 富山大学五福キャンパス(富山市)(招待講演)
  • Year and Date: 2009-09-08
• [Presentation] 絶縁体表面におけるバイオ分子の引力イメージング(招待講演) (2009)
  • Author(s): *松本卓也
  • Organizer: 第70回応用物理学学術講演会
  • Place of Presentation: 冨山大学五福キャンパス(冨山県・冨山)
  • Year and Date: 2009-09-08
• [Book] 実験物理科学シリーズ6 (2009)
  • Author(s): 松本卓也(重川秀実、吉村雅満、河津璋共編)
  • Total Pages: 425
  • Publisher: 共立出版
• [Book] 「有機分子試料測定のために」実験物理科学シリーズ6「走査プローブ顕微鏡」実践編(第2章 試料の作り方・扱い方、第3節)(重川秀実、吉村雅満、河津璋共編) (2009)
  • Author(s): 松本卓也
  • Total Pages: 425
  • Publisher: 共立出版
• [Remarks]
  • URL: http://www.chem.sci.osaka-u.ac.jp/lab/matsumoto/index.html
• [Patent(Industrial Property Rights)] Probe apparatus for measuring an electron state on a sample surface (2009)
  • Inventor(s): Takkuya Matsumoto, Tomoji Kawai
  • Industrial Property Rights Holder: Japan Science, Technology Agency
  • Filing Date: 2009-02-10
  • Overseas
• [Patent(Industrial Property Rights)] Probe apparatus for measuring anelectron state on a sample surface (2008)
  • Inventor(s): Takkuya Matsumoto, Tomoji Kawai
  • Industrial Property Rights Holder: Japan Science and Technology Agency
  • Filing Date: 2008-12-30
  • Acquisition Date: 2011-01-25
  • Overseas
• [Patent(Industrial Property Rights)] Peobe device and method ofcontrolling the same (2005)
  • Inventor(s): Takkuya Matsumoto, Yoichi Otsuka, Yasuhisa Oaitoh, Tomoji Kawai
  • Industrial Property Rights Holder: Osaka University
  • Filing Date: 2005-02-25
  • Acquisition Date: 2010-02-05
  • Overseas