Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2008: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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Research Abstract |
The electrofusion of a mammalian cell and a giant unilamellar vesicle encapsulating lyo-, cryoprotectants has been investigated. In this study, a prototype of electrofusion device allowing the fusion one-on-one pair of cell and GUV has been proposed. In addition, the designing method of electrofusion process by calculating the electric field and membrane potential during electrofusion has been developed.
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