Development of the high performance electrofusion device for loading lyo-, cryoprotective agents in vivo

• Project/Area Number: 20360094
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thermal engineering
• Research Institution: The University of Tokyo
• Principal Investigator: SHIRAKASHI Ryo The University of Tokyo, 生産技術研究所, 准教授 (80292754)
• Co-Investigator(Renkei-kenkyūsha): TAKANO Kiyoshi 東京大学, 生産技術研究所, 助教 (60302626)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2008: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
• Keywords: バイオチップ / 電気融合 / 細胞電気物性 / 膜電位 / 電気泳動 / 電場解析 / リポソーム / 細胞 / 生体熱工学

Research Abstract

The electrofusion of a mammalian cell and a giant unilamellar vesicle encapsulating lyo-, cryoprotectants has been investigated. In this study, a prototype of electrofusion device allowing the fusion one-on-one pair of cell and GUV has been proposed. In addition, the designing method of electrofusion process by calculating the electric field and membrane potential during electrofusion has been developed.

Research Products

• [Journal Article] 巨大単膜リポソーム-Jurkat細胞の電気融合に及ぼす電場の影響(パルス電場印加による巨大単膜リポソーム-Jurkat細胞対の電場・膜電位解析と融合率の測定) (2011)
  • Author(s): 白樫了, Randolph Reuss, Alexander Schulz, Vladimir L. Sukhorukov, Ulrich Zimmermann
  • Journal Title: 日本機械学会論文集(B編) Vol.77(777) Pages: 1269-1278
  • Peer Reviewed
• [Journal Article] 巨大単膜リボソームとJurkat細胞の電気融合に及ぼす電場の影響(パルス電場印加による巨大単膜リポソーム-Jurkat細胞対の電場・膜電位解析と融合率の測定) (2011)
  • Author(s): 白樫了, Randolph Reuss, Alexander Schulz, Vladimir L.Sukhorukov, Ulrich Zimmermann
  • Journal Title: 日本機械学会論文集 Volume: (掲載確定)
  • Peer Reviewed
• [Journal Article] 細胞-リボソームの電気融合における電場解析 (2010)
  • Author(s): 白樫了, Randolph Reuss, Alexander Schulz, Vladimir L.Sukhorukov, Ulrich Zimmermann
  • Journal Title: 熱工学コンファレンス講演論文集 Volume: No.10-25(2010) Pages: 165-166
• [Journal Article] Evaluation of FEM Electric Field Analysis Using the Electric Properties of Giant Unilamellar Vesicles (GUV) and Jurkat Cells Measured by Electrorotation Spectroscopy (2009)
  • Author(s): Ryo Shirakashi, Randolph Reuss, Alexander Schulz, Vladimir L. Sukhorukov, Ulrich Zimmermann
  • Journal Title: Introduction of Cryo and Lyoprotectants by Electrofusion of GUVs and Living Cells,Heat Transfer Asian Research Vol.38(3) Pages: 168-182
  • Peer Reviewed
• [Journal Article] GUV, Jurkat 細胞の電気物性測定と有限要素法による電場解析モデルの検証(GUV-細胞の電気融合による凍結\乾燥保護物質の高効率導入に関する研究) (2008)
  • Author(s): 白樫了,Randolph Reuss, Alexander Schulz,Vladimir L. Sukhorukov, Ulrich Zimmermann
  • Journal Title: 日本機械学会論文集(B編) 74巻743号 Pages: 1625-1632
  • NAID: 110006836818
  • Peer Reviewed
• [Journal Article] Jurkat細胞の電気物性測定と有限要素法による電場解析モデルの検証(GUV・細胞の電気融合による凍結・乾燥保護物質の高効率導入に関する研究) (2008)
  • Author(s): 白樫了,Randolph Reuss, Alexander Schulz, Vladimir L. Sukhorukov. Ulrich Zimmermann
  • Journal Title: 日本機械学会論文集 B74-743 Pages: 1625-1632
  • Peer Reviewed
• [Journal Article] 細胞リボソームの誘電分光測定と電場解析 (2008)
  • Author(s): 白樫了,Randolph Reuss, Alexander Schulz, Vladimir L. Sukhorukov. Ulrich Zimmermann
  • Journal Title: 第45回日本伝熱シンポジウム講演論文集 2+3 Pages: 719-720
  • Peer Reviewed
• [Presentation] 細胞-リボソームの電気融合における電場解析 (2010)
  • Author(s): 白樫了, Randolph Reuss, Alexander Schulz, Vladimir L.Sukhorukov, Ulrich Zimmermann
  • Organizer: 熱工学コンファレンス
  • Place of Presentation: 長岡技術科学大学
  • Year and Date: 2010-10-30
• [Presentation] 細胞-リポソームの電気融合における電場解析 (2010)
  • Author(s): 白樫了
  • Organizer: 熱工学コンファレンス
  • Place of Presentation: 長岡技術科学大学
  • Year and Date: 2010-10-13
• [Presentation] トレハロースと細胞凍結・乾燥保存 (2009)
  • Author(s): 白樫了
  • Organizer: 第13回トレハロースシンポジウム
  • Place of Presentation: 東京ビッグサイト
  • Year and Date: 2009-11-09
• [Presentation] Calculation of Cellular Memebrane potential by FEM Electric Field Analysis using Cellular Electric Properties from Electrorotation Spectroscopy (2008)
  • Author(s): 白樫了
  • Organizer: 2nd International Forum on Heat Transfer
  • Place of Presentation: The University of Tokyo
  • Year and Date: 2008-11-17