Ion transport control of cell membrane by developing sensing technique using evanescent wave for refractive index and electrostatic potential of cell

• Project/Area Number: 20360102
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thermal engineering
• Research Institution: Keio University
• Principal Investigator: SATO Yohei Keio University, 理工学部, 准教授 (00344127)
• Co-Investigator(Kenkyū-buntansha): MURAKAMI Toshiyuki 慶應義塾大学, 理工学部, 教授 (00255598)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2009: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2008: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: マイクロ流体デバイス / 細胞表面電位 / 細胞内屈折率 / エバネッセント波 / マイクロPIV / マイクロLIF / 細胞膜 / 二波長 / ナノLIF / アイクロPIV

Research Abstract

A non-invasive sensing technique using evanescent wave for the refractive index and electrostatic potential of cell was developed in order to control ion transport between cell and culture solution in microfluidic device. A novel technique to detect refractive index difference was proposed utilizing light scattering by evanescent wave illumination. A color imaging of electrostatic potential distribution was achieved by developing a two-color LIF technique.

Research Products

• [Journal Article] エバネッセント波および体積照射を用いた非一様ゼータ電位を有する電気浸透流の時系列速度計測 (2010)
  • Author(s): 嘉副裕,中村翼,佐藤洋平
  • Journal Title: 日本機械学会論文集B編 第76巻770号 Pages: 1455-1463
  • Peer Reviewed
• [Journal Article] Evanescent-wave/volume illuminated velocity measurements of transient eletrokinetically-driven flow with nonuniform wall electrostatic potential (2010)
  • Author(s): Yutaka Kazoe, Tsubasa Nakamura, Yohei Sato
  • Journal Title: Measurement Science and Technology Vol.21
  • Peer Reviewed
• [Journal Article] エバネッセント波および体積照射を用いた非一様ゼータ電位を有する電気浸透流の時系列速度計測 (2010)
  • Author(s): 嘉副裕, 中村翼, 佐藤洋平
  • Journal Title: 日本機械学会論文集B編 Volume: 第76巻770号 Pages: 1455-1463
  • Peer Reviewed
• [Journal Article] Evanescent-wave/volume illuminated velocity measurements of transient eletrokinetically-driven flow with nonuniform wall electrostatic potential (2010)
  • Author(s): Yutaka Kazoe, Tsubasa Nakamura, Yohei Sato
  • Journal Title: Measurement Science and Technology Volume: 21
  • Peer Reviewed
• [Journal Article] Near-Wall motion of caged fluorescent dye in microchannel flows obtained from evanescent wave molecular tagging (2010)
  • Author(s): Yuriko Senga, Tsubasa Nakamura, Hiroki Fukumura, Mitsuhisa Ichiyanagi, Yohei Sato
  • Journal Title: Journal of Fluid Science and Technology Vol.5 Pages: 192-206
  • NAID: 130000254421
  • Peer Reviewed
• [Journal Article] Three-dimensional spheroid forming lab-on-a-chip using micro-rotational flow (2010)
  • Author(s): Hiroki Ota, Ryosuke Yamamoto, Koji Deguchi, Yuta Tanaka, Yutaka Kazoe, Yohei Sato, Norihisa Miki
  • Journal Title: Sensors & Actuators, B : Chemical (掲載確定)
  • Peer Reviewed
• [Journal Article] Evanescent-wave/volume illuminated velocity measurements of transient eletrokinetically-driven flow with nonuniform wall electrostatic potential (2010)
  • Author(s): Yutaka Kazoe, Tsubasa Nakamura, Yohei Sato
  • Journal Title: Measurement Science and Technology (掲載確定)
  • Peer Reviewed
• [Journal Article] Fluorescence imaging technique of surface electrostatic potential using evanescent wave illumination (2009)
  • Author(s): Yutaka Kazoe, Shu Miyakawa, Norihisa Miki, Yohei Sato
  • Journal Title: Applied Physics Letters Vol.95
  • Peer Reviewed
• [Journal Article] A technique for monitoring multiple signals with a combination of prism-based total internal reflection fluorescence microscopy (2009)
  • Author(s): Eisuke Adachi, Yutaka Kazoe, Yohei Sato, Yuko Suzuki, Tetsumei Urano, Takehiko Ueyama, Naoaki Saito, Viacheslav O.Nikolaev, Martin J.Lohse, Makoto Tominaga, Hideo Mogami
  • Journal Title: Pflugers Archiv-European Journal of Physiology 459 Pages: 227-234
  • Peer Reviewed
• [Journal Article] Fluorescence imaging technique of surface electrostatic potential using evanescent wave illumination (2009)
  • Author(s): Yutaka Kazoe, Shu Miyakawa, Norihisa Miki, Yohei Sato
  • Journal Title: Applied Physics Letters 95 Pages: 23410401-04
  • Peer Reviewed
• [Journal Article] Water-vapor permeability control of PDMS by the dispersion of collagen powder (2009)
  • Author(s): Masatoshi Ishida, Yuhua Zhang, Yutaka Kazoe, Yohei Sato, Norihisa Miki
  • Journal Title: IEEJ Transactions on Sensors and Micromachines in press
  • NAID: 10025774649
  • Peer Reviewed
• [Journal Article] Phase separation technique for suspended particles in microchannel utilizing bilayered acoustic fields (2009)
  • Author(s): Mitsuhisa Ichiyanai, Hajjime Nakanosono, Yohei Sato, Koichi Hishida
  • Journal Title: Journal of Fluid Science and Technology 4 Pages: 1-12
  • NAID: 130000077392
  • Peer Reviewed
• [Journal Article] Quantitative evaluation of patterned self-assembled monolayers by color imaging of zeta-Potential obtained from two-color fluorescence and evanescent wave illumination (2008)
  • Author(s): Yutaka Kazoe, Shu Miyakawa, Norihisa Miki, Yohei Sato
  • Journal Title: Micro Total Analysis Systems 2008 1 Pages: 643-645
• [Presentation] Refractive index difference sensing illuminated by evanescent wave for noninvasive diagnosis of cell's pathological state (2010)
  • Author(s): 栗山怜子, 田中雄太, 佐藤洋平
  • Organizer: International Symposium on Micro-Nano Mechatronics and Human Science
  • Place of Presentation: (CD-ROM)
  • Year and Date: 2010-11-08
• [Presentation] Refractive index difference sensing illuminated by evanescent wave for noninvasive diagnosis of cell's pathological state (2010)
  • Author(s): Reiko Kuriyama, Yuta Tanaka, Yohei Sato
  • Organizer: International Symposium on Micro-Nano Mechatronics and Human Science
  • Place of Presentation: 名古屋
  • Year and Date: 2010-11-08
• [Presentation] マイクロ・ナノ輸送現象のレーザ複合計測 (2010)
  • Author(s): 菱田公一, 一柳満久, 嘉副裕, 佐藤洋平
  • Organizer: 日本機械学会第2回マイクロ・ナノ工学シンポジウム
  • Place of Presentation: 松江
  • Year and Date: 2010-10-13
• [Presentation] Fluorescence imaging technique of surface electrostatic potential using evanescent wave illumination (2010)
  • Author(s): Yohei Sato
  • Organizer: International Symposium on Micro/Nano Flow Measurement Techniques
  • Place of Presentation: 東京
  • Year and Date: 2010-09-21
• [Presentation] Nano-LIF/PIV simultaneous measurement of non-uniform wall zeta-potential and electroosmotic velocity in the mixing flow field (2010)
  • Author(s): Yuta Tananka, Yutaka Kazoe, Yohei Sato
  • Organizer: International Symposium on Micro/Nano Flow Measurement Techniques
  • Place of Presentation: 東京
  • Year and Date: 2010-09-21
• [Presentation] Transient structure of ion motion induced by electric voltage obtained from Raman imaging (2010)
  • Author(s): Tomohiro Furukawa, Yohei Sato
  • Organizer: International Symposium on Micro/Nano Flow Measurement Techniques
  • Place of Presentation: 東京
  • Year and Date: 2010-09-21
• [Presentation] ナノLIF/PIVによるマイクロチャネル壁面極近傍pHおよび速度の同時計測 (2010)
  • Author(s): 田中雄太, 嘉副裕, 佐藤洋平
  • Organizer: 第38回可視化情報シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2010-07-21
• [Presentation] 非侵襲細胞状態判別を実現するエバネッセント波照射屈折率差計測法の開発 (2010)
  • Author(s): 栗山怜子,田中雄太,佐藤洋平
  • Organizer: 第47回日本伝熱シンポジウム講演論文集(Vol.1 pp.109-110)
  • Year and Date: 2010-05-26
• [Presentation] 非侵襲細胞状態判別を実現するエバネッセント波照射屈折率差計測法の開発 (2010)
  • Author(s): 栗山怜子, 田中雄太, 佐藤洋平
  • Organizer: 第47回日本伝熱シンポジウム
  • Place of Presentation: 北海道
  • Year and Date: 2010-05-26
• [Presentation] ナノ・マイクロ熱流動センシングの最前線 (2009)
  • Author(s): 佐藤洋平, 嘉副裕
  • Organizer: 日本機械学会流体工学部門講演会
  • Place of Presentation: 名古屋工業大学(名古屋市)
  • Year and Date: 2009-11-08
• [Presentation] 高時空間分解能を有する繰り返し計測によるマイクロ化学リアクタ内速度計測 (2009)
  • Author(s): 飯野雅勇, 嘉副裕, 佐藤洋平
  • Organizer: 日本機械学会流体工学部門講演会
  • Place of Presentation: 名古屋工業大学(名古屋市)
  • Year and Date: 2009-11-08
• [Presentation] Three dimensional spheroids formation platform using micro-rotation flow (2009)
  • Author(s): H.Ota, K.Deguchi, R.Yamamoto, Y.Kazoe, K.Hishida, Y.Sato, N.Miki
  • Organizer: Micro Total Analysis Systems 2009
  • Place of Presentation: 済州島(韓国)
  • Year and Date: 2009-11-03
• [Presentation] 2色ナノLIFによる固液界面電位・表面修飾分布同時センシング (2009)
  • Author(s): 佐藤洋平, 嘉副裕
  • Organizer: 可視化情報学会全国講演会
  • Place of Presentation: 山形大学(米沢市)
  • Year and Date: 2009-10-24
• [Presentation] マイクロチャネル流ラマン分光流速計の開発 (2009)
  • Author(s): 古川朋裕, 高橋基之, 佐藤洋平
  • Organizer: 日本流体力学会年会
  • Place of Presentation: 東洋大学(東京都)
  • Year and Date: 2009-09-02
• [Presentation] 二溶液混合場における壁面ゼータ電位および電気浸透流速度の同時計測 (2009)
  • Author(s): 田中雄太, 宮川修, 嘉副裕, 佐藤洋平
  • Organizer: 第46回日本伝熱シンポジウム
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2009-06-03
• [Presentation] Quantitative evaluation of patterned self-assembled monolayers by color imaging of zeta-potential obtained from two-color fluorescence and evanescent wave illumination (2008)
  • Author(s): Yutaka Kazoe, Shu Miyakawa, Norihisa Miki, Yohei Sato
  • Organizer: Micro Total Analysis Systems(Vol.1 2008 pp.643-645)
  • Year and Date: 2008-10-13
• [Presentation] Optical measurement of chemical reaction by gas dissolution in a gas-liquid microchannel flow (2008)
  • Author(s): Issei Tsutsui, Yuriko Senga, Yohei Sato
  • Organizer: The 14th International Symposium on Applicaitons of Laser Techniques to Fluid Mechanics
  • Place of Presentation: リスボン (ポルトガル)
  • Year and Date: 2008-07-07
• [Remarks] 受賞社団法人日本伝熱学会優秀プレゼンテーション賞(2010年5月26日)
• [Remarks] ホームページ
  • URL: http://www.tfe.sd.keio.ac.jp/
• [Patent(Industrial Property Rights)] Quantitative evaluation method and device for zeta potential, pH or temperature distribution at wall surface and quantitative visualization method and device for surface modification (2010)
  • Inventor(s): 佐藤洋平、嘉副裕、宮川修
  • Industrial Property Rights Holder: 学校法人慶應義塾
  • Filing Date: 2010-04-15
• [Patent(Industrial Property Rights)] Quantitative evaluation method and device for zeta potential, pH or temperature distribution a wall surface and quantitative visualization method and device for surface modification (2010)
  • Inventor(s): 佐藤洋平, 嘉副裕, 宮川修
  • Industrial Property Rights Holder: 学校法人慶應義塾
  • Filing Date: 2010-04-15
  • Overseas
• [Patent(Industrial Property Rights)] 二色蛍光色素を用いたゼータ電位定量法 (2008)
  • Inventor(s): 佐藤洋平、嘉副裕、宮川修
  • Industrial Property Rights Holder: 学校法人慶應義塾
  • Industrial Property Number: 2008-263639
  • Filing Date: 2008-10-10
• [Patent(Industrial Property Rights)] エバネッセント照明2色蛍光による表面修飾の定量的可視化法 (2008)
  • Inventor(s): 佐藤洋平, 嘉副裕, 宮川修
  • Industrial Property Rights Holder: 学校法人慶應義塾
  • Industrial Property Number: 2008-263639
  • Filing Date: 2008-10-10