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Development of a cryogenic temperature cell operating system

Research Project

Project/Area Number 20360110
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Intelligent mechanics/Mechanical systems
Research Institution地方独立行政法人青森県産業技術センター (2010)
The University of Tokyo (2008-2009)

Principal Investigator

KUDOH Ken-ichi  地方独立行政法人青森県産業技術センター, 弘前地域研究所, 所長 (90250232)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2010: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2009: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2008: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywords生命・生体工学 / 生体生命情報工学 / 知能機械 / バイオ関連機器 / 生物・生体工学 / バイオ関連機械 / マイクロインジェクション / マイクロマニピュレータ / マイクロマニピュレーション / 凍結細胞操作 / 生体生命情報学 / バイオテクノロジー
Research Abstract

The equipment which makes a hole in the egg cell frozen using very low temperature environment was developed. And the equipment made injection of the sperm possible.
The equipment which makes a hole in the egg cell frozen using very low temperature environment was developed. And the equipment made injection of the sperm possible. Roe and a mouse ovum were used in the experiment. Each cell, it froze and a hole is made by Machining by a drill. By freezing, a soft cell become hard and processing by the drill or an Endmill is attained. It succeed in making a hole(50μand 30μ) each cell. Sperm injection was tried with the mouse egg. However, fetilzation was not able to be checked.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (19 results)

All 2011 2010 2009 2008

All Journal Article (6 results) (of which Peer Reviewed: 3 results) Presentation (13 results)

  • [Journal Article] 過重力負荷培養を伴ったマウス体外受精における透明帯穿孔法の導入-低濃度精子における受精率向上の試み-2011

    • Author(s)
      下井岳,工藤謙一,亀山祐一,橋詰良一,伊藤雅夫
    • Journal Title

      日本哺乳動物卵子学会誌

      Volume: 28(2) Pages: 372-377

    • Related Report
      2010 Final Research Report
  • [Journal Article] Sister chromatid exchange frequency in early embryos and offspring derived From IVF or ICSI in mice2010

    • Author(s)
      Gaku SHIMOI, Yuichi KAMEYAMA, Ken-ichi KUDOH, Ryoichi HASHIZUME, Masao ITOH
    • Journal Title

      Journal of Mammalian eve Research

      Volume: Vol.27, No.3 Pages: 114-121

    • NAID

      10027595031

    • Related Report
      2010 Final Research Report
  • [Journal Article] Sister chromatid exchange frequency in early embryos and offspring derived From IVF or ICSI in mice2010

    • Author(s)
      Gaku SHIMOI, Yuichi KAMEYAMA, Ken-ichi KUDOH, Ryoichi HASHIZUME, Masao ITOH
    • Journal Title

      Journal of Mammalian Ove Research

      Volume: Vol.27, No.3 Pages: 114-121

    • NAID

      10027595031

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 凍結状態の卵細胞を対象とする細胞操作法の開発2009

    • Author(s)
      工藤謙一,井上智暁,樋口俊郎
    • Journal Title

      日本冷凍空調学会

      Volume: Vol.26, No.4 Pages: 451-458

    • NAID

      10026793455

    • Related Report
      2010 Final Research Report
  • [Journal Article] 凍結状態の卵細胞を対象とする細胞操作法の開発2009

    • Author(s)
      工藤謙一, 井上智暁, 樋口俊郎
    • Journal Title

      日本冷凍空調学会論文集 Vol.26, No.4

      Pages: 81-88

    • NAID

      10026793455

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] "Effect of the Grain Boundary of Ice Crystals in a Frozen Gelatin Solution on the Dielectric Properties at a Subzero Temperature2009

    • Author(s)
      Shigeaki UENO, Ryo SHIRAKASHI, Ken-ichi KUDOH
    • Journal Title

      Biosci.Biotechnol.Biochem Vol.73

      Pages: 2478-2482

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Early development of in vitro aged eggs after intracytoplasmic sperm injection2011

    • Author(s)
      Gaku Shimoi, Masato Hayashi, Yuichi Kameyama, Ryoichi Hashizume, Ken-ichi Kudoh and Masao Ito
    • Organizer
      16th World Congress on In Vitro Fertilization
    • Place of Presentation
      Tokyo
    • Related Report
      2010 Final Research Report
  • [Presentation] 稚魚を対象とした穿刺システムの開発2009

    • Author(s)
      工藤謙一, 伊藤寿美夫, 濱崎将臣, 門村和志, 宮木廉夫
    • Organizer
      第21回「電磁力関連のダイナミクス」シンポジウム
    • Place of Presentation
      長野市
    • Year and Date
      2009-05-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] 稚魚を対象とした穿刺システムの開発2009

    • Author(s)
      工藤謙一,伊藤寿美夫,濱崎将臣,門村和志,宮木廉夫
    • Organizer
      第21回「電磁力関連のダイナミクス」シンポジウム論文集
    • Place of Presentation
      長野
    • Related Report
      2010 Final Research Report
  • [Presentation] 微小円管を利用した凍結人工授精チップの開発2009

    • Author(s)
      イウォンセ,鈴木宏俊,工藤謙一,樋口俊郎
    • Organizer
      第19回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      東広島市
    • Related Report
      2010 Final Research Report
  • [Presentation] 極低温マイクロスライサイメージングシステムの改善2009

    • Author(s)
      星野瑠威,工藤謙一,荒木徹也,相良泰行
    • Organizer
      日本食品工学会第10回(2009年)年次大会講演要旨集
    • Place of Presentation
      野々市町,石川県
    • Related Report
      2010 Final Research Report
  • [Presentation] 低温環境を利用した生体・食品への新加工技術の開発2008

    • Author(s)
      工藤謙一,鈴木宏俊,樋口俊郎
    • Organizer
      2008年度日本冷凍空調学会年次大会
    • Place of Presentation
      大阪市立大学(大阪市)
    • Year and Date
      2008-10-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] 低温環境における生体・食品への微細加工技術の開発2008

    • Author(s)
      工藤謙一,鈴木宏俊,樋口俊郎
    • Organizer
      第29回日本熱物性シンポジウム
    • Place of Presentation
      日本女子大学(東京都)
    • Year and Date
      2008-10-08
    • Related Report
      2008 Annual Research Report
  • [Presentation] 凍結細胞操作法の開発2008

    • Author(s)
      工藤謙一,鈴木宏俊,樋口俊郎
    • Organizer
      生命科学研究ネットワークシンポジウム2008
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2008-09-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] 凍結生体試料への微細穴開け装置の開発2008

    • Author(s)
      工藤謙一,鈴木宏俊,樋口俊郎
    • Organizer
      日本食品科学工学会第55回大会
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2008-09-07
    • Related Report
      2008 Annual Research Report
  • [Presentation] 遠心重力負荷を応用した低精子濃度でのマウス体外受精の試み2008

    • Author(s)
      下井岳,亀山祐一,橋詰良一,工藤謙一,伊藤雅夫
    • Organizer
      第49回日本哺乳動物卵子学会, Journal of Mammarlan Ova Research
    • Place of Presentation
      東京都
    • Related Report
      2010 Final Research Report
  • [Presentation] 凍結生体試料への微細穴開け装置の開発2008

    • Author(s)
      工藤謙一,鈴木宏俊,樋口俊郎
    • Organizer
      日本食品科学工学会第55回大会講演集
    • Place of Presentation
      京都市
    • Related Report
      2010 Final Research Report
  • [Presentation] 低温環境における生体・食品への微細加工技術の開発2008

    • Author(s)
      工藤謙一,鈴木宏俊,樋口俊郎
    • Organizer
      第29回日本熱物性シンポジウム講演論文集
    • Place of Presentation
      東京都
    • Related Report
      2010 Final Research Report
  • [Presentation] 低温環境を利用した生体・食品への新加工技術の開発2008

    • Author(s)
      工藤謙一,鈴木宏俊,樋口俊郎
    • Organizer
      2008年度日本冷凍空調学会年次大会講演論文集
    • Place of Presentation
      大阪市
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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