| Project/Area Number |
20360238
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Civil and environmental engineering
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SATO Hiroyasu The University of Tokyo, 大学院・新領域創成科学研究科, 准教授 (90251347)
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| Co-Investigator(Kenkyū-buntansha) |
MINO Takashi 東京大学, 大学院・新領域創成科学研究科, 教授 (60166098)
NAKAI Hiroshi 東北大学, 大学院・農学研究科, 教授 (80155655)
OTAWA Kenichi 東北大学, 大学院・農学研究科, 助教 (00451550)
TADA Chika 東北大学, 大学院・農学研究科, 准教授 (30413892)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2008: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
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| Keywords | 生物学的廃水処理 / 活性汚泥 / バクテリオファージ / 宿主 / 溶菌 / 下水処理 / パルスフィールドゲル電気泳動法 / DNAポリメラーゼ / 宿主細菌 |
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| Research Abstract |
The influence of bacteriophages on wastewater treatment was investigated, together with the development of methods to analyze bacteriophages. Host DNA was detected in the lysate of phages, and the finding lead to the possibility to identify the lysed host by analyzing DNA in supernatant of wastewater treatment processes. It was estimated that around 1 to several % of bacteria are daily lysed by phages, and it may be possible to use phages to reduce waste sludge. Phage DNA was resistant to digestion by restriction enzymes, suggesting the complex relationship between phages and host. Yet, further compilation of case studies is still needed to know the impact of phages on wastewater treatment.
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