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Study on inactivation and removal efficacy of emerging infectious microorganisms in effluent waters

Research Project

Project/Area Number 20360243
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Civil and environmental engineering
Research InstitutionAzabu University

Principal Investigator

MORITA Shigemitsu  Azabu University, 環境保健学部, 准教授 (50318888)

Co-Investigator(Kenkyū-buntansha) HASHIMOTO Atsushi  国立阿南工業高等専門学校, 准教授 (30332068)
OGUMA KUMIKO  東京大学, 大学院・工学系研究科, 講師 (00361527)
MINAKUCHI Moeko  筑波大学, 大学院・生命システム医学専攻, 博士後期課程3年
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2010: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2009: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2008: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Keywords新興病原微生物 / 原虫 / ウイルス / 紫外線 / E型肝炎ウイルス / クリプトスポリジウム / 排水処理 / 除去・不活化
Research Abstract

We determined concentrations of Cryptosporidium oocysts and Giardia cysts in raw sewage and treated sewage effluent to calculate removal rates in swine sewage treatment plants. Removal rates calculated from the above average values were 2.14 log for Cryptosporidium oocysts and 2.42 log for Giardia cysts. It was clear that both protozoa could be effectively removed by sewage treatment.
Three years studies were carried out in the concentration of hepatitis E virus (HEV) in shellfish. The concentration of each virus was determined using real-time quantitative PCR after concentration of the water samples using negatively-charged membranes. HEV were detected in shellfish at the mouth of a river.
C.parvum oocysts and B.subtilis were evaluated for infectivity with infectivity assay after treatment by ultraviolet irradiation alone, contact with oxidizing agent alone, and contact with oxidizing agent after UV irradiation. The log reduction with combined treatment of oxidizing agent after UV irradiation was 1.7-100 times greater than the sum of the log reductions by these separate treatments under the same conditions. The results indicate that the combination of oxidizing agent after UV irradiation has a synergistic effect on microorganism inactivation.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (24 results)

All 2011 2010 2009 2008

All Journal Article (5 results) (of which Peer Reviewed: 2 results) Presentation (18 results) Book (1 results)

  • [Journal Article] 水源における病原微生物濃度の予測と発生源対策効果の推定2011

    • Author(s)
      岸田直裕, 原本英司, 浅見真理, 森田重光, 秋葉道宏
    • Journal Title

      用水と廃水

      Volume: (accept)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Annual and diurnal profiles of Cryptosporidium and Giardia in river water in Japan2011

    • Author(s)
      N.Kishida, S.Morita, E.Haramoto M.Akiba
    • Journal Title

      Journal of Water Environment Technology

      Volume: (accept)

    • NAID

      130000867946

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] FISH-蛍光抗体染色法を併用したクリプトスポリジウムの判別の容易化2009

    • Author(s)
      橋本温,森田重光,平田強
    • Journal Title

      日本水環境学会誌 5

      Pages: 267-272

    • Related Report
      2010 Final Research Report
  • [Journal Article] Detection of HEV in environmental water2008

    • Author(s)
      Morita, S., Tanimoto J., Urakami I., Katayama H., Ohgaki S., Itoh S., Hirata T.
    • Journal Title

      Proceedings of World Water Congress of the International Water Association (in CD)

      Pages: 7-7

    • Related Report
      2010 Final Research Report
  • [Journal Article] E型肝炎ウイルスの分布と廃水処理による環境放出の制御2008

    • Author(s)
      森田重光
    • Journal Title

      PIG JOURNAL 1

      Pages: 18-21

    • Related Report
      2010 Final Research Report
  • [Presentation] 海水中に含まれる微生物の促進酸化処理2011

    • Author(s)
      大槻弥緒, 大瀧雅寛, 小熊久美子, 森田重光
    • Organizer
      日本水環境学会
    • Place of Presentation
      (震災のため中止となったが学会判断で発表扱いとなった)
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 養豚環境における豚サーコウイルス2型(PCV2)の移行について2011

    • Author(s)
      森田重光, 伊東正吾, 片山浩之, 伊東貢
    • Organizer
      日本水環境学会
    • Place of Presentation
      (震災のため中止となったが学会判断で発表扱いとなった)
    • Year and Date
      2011-03-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] 海水中に含まれる微生物の促進酸化処理2011

    • Author(s)
      大槻弥緒,大瀧雅寛,出口憲一郎,浦上逸男,小熊久美子,森田重光
    • Organizer
      第44回日本水環境学会
    • Place of Presentation
      札幌
    • Related Report
      2010 Final Research Report
  • [Presentation] 水道水源における水系感染性ウイルスの濃度変動とその影響因子2011

    • Author(s)
      岸田直裕,森田久男,浅見真理,秋葉道宏,原本英司,森田重光
    • Organizer
      第44回日本水環境学会
    • Place of Presentation
      札幌
    • Related Report
      2010 Final Research Report
  • [Presentation] 養豚環境における豚サーコウイルス2型(PCV2)の移行について2011

    • Author(s)
      森田重光,伊東正吾,片山浩之,岸田直裕,秋葉道宏,伊藤貢
    • Organizer
      第44回日本水環境学会
    • Place of Presentation
      札幌
    • Related Report
      2010 Final Research Report
  • [Presentation] 水環境および貝類からのE型肝炎ウイルスの検出状況2010

    • Author(s)
      森田重光, 小熊久美子, 岸田直裕, 秋葉道宏
    • Organizer
      第44回に本水環境学会年会
    • Place of Presentation
      福岡大学(福岡市)
    • Year and Date
      2010-03-16
    • Related Report
      2009 Annual Research Report
  • [Presentation]2010

    • Organizer
      19^<th> Symposium of Japan-Korea Water Environment
    • Place of Presentation
      Hiroshima
    • Related Report
      2010 Final Research Report
  • [Presentation] 豚舎および畜産排水処理施設の空気からのPCV2ゲノムの検出2010

    • Author(s)
      森田重光,伊藤貢,松原康一,伊東正吾
    • Organizer
      PCC豚病症例検討会
    • Place of Presentation
      相模原
    • Related Report
      2010 Final Research Report
  • [Presentation] 組み合わせ処理による促進酸化効果2010

    • Author(s)
      森田重光
    • Organizer
      第56回日本水環境学会シンポジウム
    • Place of Presentation
      京都
    • Related Report
      2010 Final Research Report
  • [Presentation] Detection of PCV2 genomes from the air of swinery and swinery-equipped wastewater treatment facility2009

    • Author(s)
      K.Matsubara, S.Morita, M.Itoh, K.Oguma, H.Katayama, S.Ohgaki
    • Organizer
      The 4^<th> Congress of Asian Pig Veterinary Society
    • Place of Presentation
      Tsukuba International Congress Center(Tsukuba)
    • Year and Date
      2009-10-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Behavior of Adenovirus, Sapovirus and Hepatitis E Virus in Aquatic Environment2009

    • Author(s)
      S.Morita, K.Oguma, T.Hirata
    • Organizer
      The 3^<rd> IWA-ASPIRE Conference & Exhibition
    • Place of Presentation
      Taipei International Convention Center(Taipei)
    • Year and Date
      2009-10-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] CryptosporidiumオーシストおよびGiardiaシストの精製方法の検討2009

    • Author(s)
      石井孝明, 金田聖子, 吉田伸広, 森田重光, 平田強
    • Organizer
      日本水環境学会年会
    • Place of Presentation
      山口大学
    • Year and Date
      2009-03-18
    • Related Report
      2008 Annual Research Report
  • [Presentation] 畜産廃水処理によるE型肝炎ウイルスの除去2009

    • Author(s)
      森田重光, 小熊久美子, 片山浩之, 平田強
    • Organizer
      日本水環境学会年会
    • Place of Presentation
      山口大学
    • Year and Date
      2009-03-16
    • Related Report
      2008 Annual Research Report
  • [Presentation] 畜産廃水処理によるE型肝炎ウイルス(HEV)の除去2009

    • Author(s)
      森田重光,小熊久美子,片山浩之,平田強
    • Organizer
      第43回日本水環境学会
    • Place of Presentation
      山口
    • Related Report
      2010 Final Research Report
  • [Presentation] Behavior of Adenovirus, Sapovirus and Hepatitis E virus in Aquatic Environment2009

    • Author(s)
      森田重光, 小熊久美子
    • Organizer
      International Water Association-ASPIRE Conference
    • Place of Presentation
      Taipei
    • Related Report
      2010 Final Research Report
  • [Presentation] Detection of HEV in environmental water2008

    • Author(s)
      Morita, S.
    • Organizer
      International Water Association World Water Congress and Exhibition
    • Place of Presentation
      Austria Center Vienna
    • Year and Date
      2008-09-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] CryptosporidiumおよびGiardiaの定量法に関する研究2008

    • Author(s)
      森田重光,岩崎好夏,小杉沙織,石井孝明,平田強
    • Organizer
      第42回日本水環境学会
    • Place of Presentation
      名古屋
    • Related Report
      2010 Final Research Report
  • [Presentation] Detection of HEV in environmental water2008

    • Author(s)
      Morita, S., Tanimoto J., Urakami I., Katayama, H., Ohgaki S., Itoh, S., Hirata T.
    • Organizer
      World Water Congress of the International Water Association
    • Place of Presentation
      Vienna
    • Related Report
      2010 Final Research Report
  • [Book] 水への応用(紫外線照射)2008

    • Publisher
      技法堂
    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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