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Structural regulation of amyloid forming proteins with pyrroloquinoline quinone

Research Project

Project/Area Number 20360369
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

SODE Koji  Tokyo University of Agriculture and Technology, 大学院・工学研究院, 教授 (10187883)

Co-Investigator(Kenkyū-buntansha) IKEBUKURO Kazunori  東京農工大学, 大学院・工学研究院, 教授 (70251494)
TSUGAWA Wakako  東京農工大学, 大学院・工学研究院, 准教授 (80376871)
FERRI Stefano  東京農工大学, 大学院・工学研究院, 助教 (90334474)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2008: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Keywords生物機能工学 / アミロイド / αシヌクレイン / Aβ / プリオン / 構造制御 / 蛋白質 / バイオテクノロジ / 生体分子 / PQQ / 細胞毒性 / 膜感受性蛍光色素 / バイオテクノロジー / ペプチド / 凝集線維化 / 神経変性疾患
Research Abstract

The development of small molecules inhibiting amyloid formation of various proteins, such as alpha synuclein, the causative protein of Parkinson's disease, were investigated. The partial peptides modified with pyrroloquinoline quinone (PQQ) were found to specifically inhibit the amyloid formation of parental protein, but not other amyloid forming proteins.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (21 results)

All 2010 2009 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (11 results) Book (2 results)

  • [Journal Article] Pyrroloquinoline Quinone Inhibits the Fibrillation of Amyloid Proteins2010

    • Author(s)
      J.Kim, M.Kobayashi, M.Fukuda, D.Ogasawara, N.Kobayashi, S.Han, C.Nakamura, M.Inada, C.Miyaura, K.Ikebukuro, K.Sode
    • Journal Title

      Prion 4(1)

      Pages: 26-31

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The inhibitory effect of pyrroloquinoline quinine on the amyloid formation and cytotoxicity of truncated alpha-synuclein2010

    • Author(s)
      J.Kim, R.Harada, M.Kobayashi, N.Kobayahi, K.Sode
    • Journal Title

      Molecular Neurodegeneration 20

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The inhibitory effect of pyrroloquinoline quinine on the amyloid formation and cytotoxicity of truncated alpha-synuclein2010

    • Author(s)
      J.Kim, R.Harada, M.Kobayashi, N.Kobayahi, K.Sode
    • Journal Title

      Molecular Neurodegeneration

      Volume: 20 Pages: 20-20

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pyrroloquinoline Quinone Inhibits the Fibrillation of Amyloid Proteins2010

    • Author(s)
      Jihoon Kim, Masaki Kobayashi, Makoto Fukuda, Daisuke Ogasawara, Natsuki Kobayashi, Sungwoong Han, Chikashi Nakamura, Masaki Inada, Chisato Miyaura, Kazunori Ikebukuro, Koji Sode
    • Journal Title

      Prion 2010 Jan.4^<th> 4(1)(Epub ahead of print)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The inhibition of amyloid fibrillation using the proteolytic products of PQQ-modified α-synuclein2009

    • Author(s)
      N.Kobayashi, J.Kim, K.Ikebukuro, K.Sode
    • Journal Title

      The Open Biotechnology J. 3

      Pages: 40-45

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Effect of Amino Acid Substitution in the Imperfect Repeat Sequences of α-Synuclein on Fibrillation2009

    • Author(s)
      R.Harada, N.Kobayashi, J.Kim, C.Nakamura, S.Han, K.Ikebukuro, K.Sode
    • Journal Title

      Biochim Biophys Acta. 1792(10)

      Pages: 998-1003

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Effect of Amino Acid Substitution in the Imperfect Repeat Sequences of α-Synuclein on Fibrillation2009

    • Author(s)
      Ryuichi Harada, Natsuki Kobayashi, Jihoon Kim, Chikashi Nakamura, Sungwoong Han, Kazunori Ikebukuro, Koji Sode
    • Journal Title

      Biochim Biophys Acta. 1792(10)

      Pages: 998-1003

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The inhibition of amyloid fibrillation using the proteolytic products of PQQ-modified α-synuclein2009

    • Author(s)
      Natsuki, Kobayashi, Jihoon Kim, Kazunori, Ikebukuro, Koji Sode
    • Journal Title

      The Open Biotechnology Journal (In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] アミロイド形成蛋白質細胞毒性用蛍光バイオセンシングの開発2010

    • Author(s)
      金志勲, 戸田礼, 佐々木泰彦, 早出広司
    • Organizer
      電気化学会
    • Place of Presentation
      神奈川工科大学、厚木
    • Year and Date
      2010-09-02
    • Related Report
      2010 Final Research Report
  • [Presentation] アミロイド形成蛋白質細胞毒性用蛍光バイオセンシングの開発2010

    • Author(s)
      金志勲, 戸田礼, 佐々木泰彦, 早出広司
    • Organizer
      電気化学会
    • Place of Presentation
      神奈川工科大学(厚木)
    • Year and Date
      2010-09-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] The development of a novel theranostic platform for cytotoxicity evaluation of amyloid-forming neurodegenerative disease causative proteins2010

    • Author(s)
      J Kim, R Harada, N Kobayashi, K Ikebukuro, K Sode
    • Organizer
      Biosensor 2010
    • Place of Presentation
      Glasgo, UK
    • Year and Date
      2010-05-26
    • Related Report
      2010 Final Research Report
  • [Presentation] The development of a novel theranostic platform for cytotoxicity evaluation of a myloid-forming neurodegenerative disease causative proteins2010

    • Author(s)
      J Kim, R Harada, N Kobayashi, KIkebukuro,K Sode
    • Organizer
      Biosensor 2010
    • Place of Presentation
      イギリス グラスゴー
    • Year and Date
      2010-05-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] アミロイド蛋白質細胞毒性バイオセンシングのセラノスティクスへの応用2010

    • Author(s)
      金志勲、小林夏季、池袋一典、早出広司
    • Organizer
      日本化学会(CSJ Student Presentation Award 2010)
    • Place of Presentation
      近畿大学
    • Related Report
      2010 Final Research Report
  • [Presentation] The prevention of α-synuclein amyloid formation using PQQ-modified partial peptide fragments : for target specific prevention of the amyloid formation2009

    • Author(s)
      N.Kobayashi, K.Sode
    • Organizer
      9th International Conference AD/PD 2009
    • Place of Presentation
      Prague (Czech Republic)(Best Poster Award)
    • Year and Date
      2009-03-15
    • Related Report
      2010 Final Research Report
  • [Presentation] The prevention of α-synuclein amyloid formation using PQQ-modified partial peptide fragments: for target specific prevention of the amyloid formation2009

    • Author(s)
      Natsuki Kobayashi
    • Organizer
      9th International Conference AD/PD
    • Place of Presentation
      Prague (Czech Republic)
    • Year and Date
      2009-03-15
    • Related Report
      2008 Annual Research Report
  • [Presentation] 膜蛋白質感受性色素を用いたアミロイド形成蛋白質の新規細胞毒性評価用バイオセンシングシステムの開発2009

    • Author(s)
      金志勲、小林夏季、池袋一典、早出広司
    • Organizer
      蛋白質科学会
    • Place of Presentation
      熊本(ポスター賞)
    • Related Report
      2010 Final Research Report
  • [Presentation] 蛍光偏向解消法を用いたαシヌクレインオリゴマーの検出2009

    • Author(s)
      原田龍一、小林夏季、金志勲、池袋一典、早出広司
    • Organizer
      蛋白質科学会
    • Place of Presentation
      熊本
    • Related Report
      2010 Final Research Report
  • [Presentation] 切断型αシヌクレインの凝集・線維化におけるピロロキノリンキノン(PQQ)の影響2009

    • Author(s)
      Kim Jihoon、小林雅樹、小林夏季、早出広司
    • Organizer
      蛋白質科学会
    • Place of Presentation
      熊本全日空ホテル(熊本県)
    • Related Report
      2009 Annual Research Report
  • [Presentation] 蛍光偏向解消法を用いたαシヌクレインオリゴマーの検出2009

    • Author(s)
      原田龍一、小林夏季、金志勲、池袋一典、早出広司
    • Organizer
      蛋白質科学会
    • Place of Presentation
      熊本全日空ホテル(熊本県)
    • Related Report
      2009 Annual Research Report
  • [Book] Nanomedicine and the nervous system.(Biomolecular engineering for the regulation of alpha-synuclein nanostructure~toward the development of alpha-synuclein targeting nanomedicine~)

    • Author(s)
      N.Kobayashi, J.Kim, K.Sode
    • Publisher
      Science Publishers(in press)
    • Related Report
      2010 Final Research Report
  • [Book] Nanomedicince and the nervous system.以下の章を分担Biomolecular engineering for the regulation of alpha-synuclein nanostructure~toward the development of alpha-synuclein targeting nanomedicine~

    • Author(s)
      Natsuki Kobayashi, Jihoon Kim, Koii Sode
    • Publisher
      Science Publishers
    • Related Report
      2010 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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