MAGNETIC FORCE-BASED-DROPLET-HANDLING SYSTEM FOR SINGLE CELL DIGITAL PCR ANALYSIS

• Project/Area Number: 20360371
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Nagoya University
• Principal Investigator: OKOCHI Mina Nagoya University, 工学研究科, 准教授 (70313301)
• Co-Investigator(Kenkyū-buntansha): HONDA Hiroyuki 名古屋大学, 大学院・工学研究科, 教授 (70209328) ; KATO Ryuji 名古屋大学, 大学院・工学研究科, 助教 (50377884) ; SHIKIDA Mitsuhiro 名古屋大学, 大学院・工学研究科, 准教授 (80273291) ; MIZUTANI Takashi 名古屋大学, 大学院・工学研究科, 教授 (70273290)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2008: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: バイオセンサ / 一細胞解析 / 液滴 / 磁性搬送 / 磁性粒子 / 1細胞解析 / PCR / 遺伝子発現解析 / 細胞

Research Abstract

A droplet-based single cell analysis system was developed employing magnetic force-based droplet-handling system. The droplets containing magnetic beads were magnetically controlled and coalesced with another droplet in the oil phase, and all the reactions for RT-PCR including single cell manipulation, cell lysis, cDNA synthesis, and PCR amplification steps were integrated on-chip and applied for detection of cancer cells.

Research Products

• [Journal Article] Droplet introduction into a pen-shaped portable reaction system by gravity and interfacial forces. (2011)
  • Author(s): Shikida M., Inagaki N., Okochi M.,Honda H., Sato K.
  • Journal Title: Micro, Nano Letters 6 Pages: 253-256
  • Peer Reviewed
• [Journal Article] Screening of ZnO-high affinity peptide using spot-synthesized peptide arrays and computational analysis (2010)
  • Author(s): Okochi M., Ogawa M., Kaga C., Sugita T., Tomita T., Kato R., Honda H.
  • Journal Title: Acta Biomaterialia 6 Pages: 2301-2306
  • Peer Reviewed
• [Journal Article] Peptide Array-Based Characterization and Design of ZnO-High Affinity Peptides. (2010)
  • Author(s): Okochi, M., Tomoya Sugita, Seiji Furusawa, Mitsuo Umetsu, Adschiri T., Honda H.
  • Journal Title: Biotechnol.Bioeng. 106 Pages: 845-851
  • Peer Reviewed
• [Journal Article] Fabrication process of carbon nanotube field effect transistors using atomic layer deposition passivation for biosensors. (2010)
  • Author(s): Nakashima Y., Ohno Y., Kishimoto S.Okochi M., Honda H., Mizutani T.
  • Journal Title: J.Nanosci.Nanotechnol. 10 Pages: 3805-3809
  • Peer Reviewed
• [Journal Article] Droplet-based gene expression analysis using a device with magnetic force-based droplethandling system (2010)
  • Author(s): Okochi M., Tsuchiya H., Kumazawa F., Shikida M., Honda H.
  • Journal Title: J.Biosci.Bioeng. 109 Pages: 193-197
  • NAID: 110007573253
  • Peer Reviewed
• [Journal Article] Fabrication process of carbon nanotube field effect transistors using atomic layer deposition passivation for biosensors. (2010)
  • Author(s): Nakashima Y., et al.
  • Journal Title: Journal of Nanoscience and Nanotechnology Volume: 10 Pages: 3805-3809
  • Peer Reviewed
• [Journal Article] Screening of ZnO-high affinity peptide using spot-synthesized peptide arrays and computational analysis (2010)
  • Author(s): Okochi M., et al.
  • Journal Title: ActaBiomaterialia Volume: 6 Pages: 2301-2306
  • Peer Reviewed
• [Journal Article] Peptide Array-Based Characterization and Design of ZnO-High Affinity Peptides (2010)
  • Author(s): Okochi M., et al.
  • Journal Title: Biotechnology and Bioengineering Volume: 106 Pages: 845-851
  • Peer Reviewed
• [Journal Article] Fabrication process of carbon nanotube field effect transistors using atomic layer deposition passivation for biosensors (2010)
  • Author(s): Yasuhiro Nakashima, et al.
  • Journal Title: J.Nanosci.Nanotechnol. Volume: 10 Pages: 3805-3809
  • Peer Reviewed
• [Journal Article] Droplet-based gene expression analysis using a device with magnetic force-based-droplet-handling system (2010)
  • Author(s): Mina Okochi, et al.
  • Journal Title: J.Biosci.Bioeng. Volume: 109 Pages: 193-197
  • Peer Reviewed
• [Journal Article] Three-dimensional cell culture array using magnetic force-based cell patterning for analysis of invasive capacity of BALB/3T3/v-src. (2009)
  • Author(s): Okochi M., Takano S., Isaji Y., Senga T.,Hamaguchi M., Honda H.
  • Journal Title: Lab.Chip 9 Pages: 3378-3384
  • Peer Reviewed
• [Journal Article] Agitation of magnetic beads by multi-layered flat coils. (2009)
  • Author(s): Shikida M., Koyama M., Nagao N., Imai R., Honda H., Okochi M,, Tsuchiya H., Kazuo Sato.
  • Journal Title: Sens.Actuat-B : Chemical 137 Pages: 774-780
  • Peer Reviewed
• [Journal Article] Magnetic force-based lab-on-a-chip for single cell analysis in a droplet. (2009)
  • Author(s): Okochi M,, Tsuchiya H., Kumazawa F., Shikida M., Honda H.
  • Journal Title: ECS Transactions 16(17) Pages: 15-20
  • Peer Reviewed
• [Journal Article] Preparation of L929 cell array by magnetic cell labeling method with unmodified colloidal magnetite nanoparticles for single cell detection of cell response induced by TNF-α. (2009)
  • Author(s): Kiode H., Isaji Y., Okochi M., Honda H.
  • Journal Title: J.Chem.Eng.Jpn. 42(4)
  • Peer Reviewed
• [Journal Article] Threedimensional cell culture array using magnetic force-based cell patterning for analysis of invasive capacity of BALB/3T3/v-src. (2009)
  • Author(s): Okochi M., Takano S., Isaji Y., Senga T., Hamaguchi M., Honda H.
  • Journal Title: Lab.Chip 9 Pages: 3378-3384
  • Peer Reviewed
• [Journal Article] Application of magnetic force-based cell patterning for controlling cell-cell interactions in angiogenesis. (2009)
  • Author(s): Ino K., Okochi M., Honda H.
  • Journal Title: Biotechnol.Bioeng. 102 Pages: 882-890
  • Peer Reviewed
• [Journal Article] Three-dimensional cell culture array using magnetic force-based cell patterning for analysis of invasive capacity of BALB/3T3/v-src (2009)
  • Author(s): Mina Okochi, et al.
  • Journal Title: Lab.Chip Volume: 9 Pages: 3378-3384
  • Peer Reviewed
• [Journal Article] Application of magnetic force-based cell patterning for controlling cell-cell interactions in angiogenesis (2009)
  • Author(s): Kosuke Ino, et al.
  • Journal Title: Biotechnol. Bioeng. 102 Pages: 882-890
  • Peer Reviewed
• [Journal Article] Preparation of L929 cell array by magnetic cell labeling method with unmodified colloidal magnetite nanoparticles for single cell detection of cell response induced by TNF-α. (2009)
  • Author(s): Hironobu Kiode, et al.
  • Journal Title: J. Chem. Eng. Jpn. 42(in press)
  • Peer Reviewed
• [Journal Article] Magnetic force-based lab-on-a-chip for single eell analysis in a droplet (2009)
  • Author(s): Mina Okochi, et al.
  • Journal Title: ECS Transactions - Honolulu, HI 13(in press)
  • Peer Reviewed
• [Journal Article] Agitation of magnetic beads by multi-layered flat coils (2009)
  • Author(s): Mitsuhiro Shikida, et al.
  • Journal Title: Sens. Actuat-B : Chemical 137 Pages: 774-780
  • Peer Reviewed
• [Journal Article] Fabrication of vertically-aligned carbon nanotube electrodes using grid-inserted plasma-enhanced chemical vapor deposition for chemical sensors. (2008)
  • Author(s): Kojima, Y. Kishimoto S., Okochi M., Honda H., Mizutani T.
  • Journal Title: Jpn J.Appl.Phys. 47 Pages: 2028-2031
  • Peer Reviewed
• [Journal Article] palmtop-sized rotary-drive-type biochemical analysis system by magnetic bead handling. (2008)
  • Author(s): Shikida M., Nagao N., Imai R., Honda H., Okochi M., Ito H., Sato K. A
  • Journal Title: J.Micromech.Microeng. 18 Pages: 35034-35042
  • Peer Reviewed
• [Journal Article] Cell culture arrays using magnetic force-based cell patterning force-based cell patterning for dynamic single cell analytysis. (2008)
  • Author(s): Ino K., Okochi M., Konishi N., Nakatochi M., Imai R., Shikida M., Ito A., Honda H.
  • Journal Title: Lab.Chip. 8 Pages: 134-142
  • Peer Reviewed
• [Journal Article] On-chip polymerase chain reaction microdevice employing a magnetic dropletmanipulation system. (2008)
  • Author(s): Tsuchiya H., Okochi M., Nagao N., Shikida M., Honda H.
  • Journal Title: Sens.Actuator B-Chem. 130 Pages: 583-588
  • Peer Reviewed
• [Journal Article] Fabrication of vertically-aligned carbon nanotube electrodes using grid-inserted plasma-enhanced chemical vapor deposition for chemical sensors (2008)
  • Author(s): Yoshihiro Kojima, et al.
  • Journal Title: Jpn J. Appl. Phys. 47 Pages: 2028-2031
  • Peer Reviewed
• [Journal Article] Fabrication of a pen-shaped portable biochemical reaction system based on magnetic bead manipulation
  • Author(s): Shikida M., Inagaki N., Okochi M., Honda H., Sato K.
  • Journal Title: J.Micromech.Microeng. in press
  • Peer Reviewed
• [Presentation] Evaluation of invasive capacity of tumor cells using magnetically micropatterned multicellular spheroid (2011)
  • Author(s): Okochi M., Matumura T., Honda H.
  • Organizer: 5th International workshop on single cell analysis
  • Place of Presentation: The University of Tokyo
  • Year and Date: 2011-03-04
• [Presentation] Evaluation of invasive capacity of tumor cells using magnetically micropatterned multicellular spheroid (2011)
  • Author(s): Okochi M., et al.
  • Organizer: 5^<th> International workshop on single cell analysis
  • Place of Presentation: The University of Tokyo
  • Year and Date: 2011-03-04
• [Presentation] Three dimensional cell culture system using magnetic force-based cell patterning for analysis of invasive capacity of tumor cells (2010)
  • Author(s): Okochi M. Honda H.
  • Organizer: 4th International, Symposium on Nanomedicine
  • Place of Presentation: 岡崎分子科学研究所
  • Year and Date: 2010-11-30
• [Presentation] Three dimensional cell culture system using magnetic force-based cell patterning for analysis of invasive capacity of tumor cells (2010)
  • Author(s): Okochi M., et al
  • Organizer: 4^<th> International Symposium on Nanomedicine
  • Place of Presentation: 岡崎分子科学研究所
  • Year and Date: 2010-11-30
• [Presentation] マイクロウェルチャンバーを用いた磁性液滴搬送デバイスによる細胞解析 (2010)
  • Author(s): 大河内美奈、熊澤史貴、手島翠、古池真司、本多裕之
  • Organizer: 日本生物工学会2010年度大会
  • Place of Presentation: 宮崎シーガイヤ
  • Year and Date: 2010-10-29
• [Presentation] 磁気細胞パターニング法を用いた生体模倣条件下でのがん細胞挙動評価 (2010)
  • Author(s): 松村拓、小出寛展、大河内美奈、本多裕之
  • Organizer: 化学工学会第42回秋季大会
  • Place of Presentation: 京都大学(チ)
  • Year and Date: 2010-09-06
• [Presentation] 磁気剣山状デバイスを用いた単-細胞機能解析 (2010)
  • Author(s): 小出寛展、伊佐治弥生、大河内美奈、本多裕之
  • Organizer: 化学工学会第42回秋季大会
  • Place of Presentation: 京都大学
  • Year and Date: 2010-09-06
• [Presentation] 磁気細胞パターニング法を用いた生体模倣条件下でのがん細胞挙動評価 (2010)
  • Author(s): 松村拓, 他
  • Organizer: 化学工学会第42回秋季大会
  • Place of Presentation: 京都大学
  • Year and Date: 2010-09-06
• [Presentation] 磁性粒子を用いた細胞解析法の開発 (2010)
  • Author(s): 大河内美奈、本多裕之
  • Organizer: 第17回HAB研究機構学術年会
  • Place of Presentation: 昭和大学
  • Year and Date: 2010-05-21
• [Presentation] 3次元細胞アレイによる腫瘍細胞の浸潤評価 (2010)
  • Author(s): 小出寛展、松村拓、大河内美奈、本多裕之
  • Organizer: 化学工学会第75年会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2010-03-18
• [Presentation] 磁性粒子を用いた細胞解析法の開発 (2010)
  • Author(s): 大河内美奈, 他
  • Organizer: バイオプロセッシング研究会
  • Place of Presentation: 東京
  • Year and Date: 2010-02-23
• [Presentation] Magnetic manipulation device for the optimization of cell processing (2009)
  • Author(s): Ito H., Kato R., Okochi M., Honda H.
  • Organizer: APBioChEC2009
  • Place of Presentation: Kobe
  • Year and Date: 2009-11-26
• [Presentation] 細胞間コミュニケーションを保った細胞アレイの作成と機能解析 (2009)
  • Author(s): 本多裕之、大河内美奈、伊野浩介、高野翔、小出寛展
  • Organizer: 日本生物工学会2009年度大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-25
• [Presentation] 磁化細胞アレイを用いた細胞応答の検出 (2009)
  • Author(s): 小出寛展、松村拓、高野翔、千賀威、大河内美奈、本多裕之
  • Organizer: 日本生物工学会2009年度大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-23
• [Presentation] 磁力を用いた液滴送液システムによる1細胞遺伝子発現解析デバイスの開発 (2009)
  • Author(s): 熊澤史貴、大河内美奈、式田光宏、本多裕之
  • Organizer: 日本生物工学会2009年度大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-23
• [Presentation] 培養条件検討への細胞磁気マニピュレーションの導入 (2009)
  • Author(s): 伊藤博史、加藤竜司、大河内美奈、本多裕之
  • Organizer: 化学工学会第41回秋季大会
  • Place of Presentation: 広島大学
  • Year and Date: 2009-09-17
• [Presentation] 磁性微粒子を用いた液滴搬送システムによるイノムアッセイチップの開発 (2009)
  • Author(s): 大河内美奈、熊澤史貴、シャムスーディンヌルヒダヤ、式田光宏、本多裕之
  • Organizer: 化学工学会第41回秋季大会
  • Place of Presentation: 広島大学
  • Year and Date: 2009-09-16
• [Presentation] 磁性微粒子を用いた液滴ハンドリングシステムによる-細胞RT-PCR (2009)
  • Author(s): 熊澤史貴、大河内美奈、土屋裕義、式田光宏、本多裕之
  • Organizer: 日本化学会第89年会
  • Place of Presentation: 日本大学
  • Year and Date: 2009-03-29
• [Presentation] 磁気細胞パターニング法を用いた3次元がん細胞挙動評価モデルの構築 (2009)
  • Author(s): 高野翔、大河内美奈、千賀威、本多裕之
  • Organizer: 化学工学会第74年会
  • Place of Presentation: 神奈川工科大学
  • Year and Date: 2009-03-18
• [Presentation] Palm-top aized magnetic manipulation device to assist optimization of cell proces sing conditions in small scale (2009)
  • Author(s): Hiroshi Ito, et al.
  • Organizer: Termis
  • Place of Presentation: 韓国ソウル
• [Presentation] 磁力を用いた液滴送液システムによる1細胞遺伝子発現解析デバイスの開発 (2009)
  • Author(s): 熊澤史貴, 他
  • Organizer: 日本生物工学会第61回大会
  • Place of Presentation: 名古屋大学
• [Presentation] Magnetic force-based droplet manipulation system for gene expression analysis. (2008)
  • Author(s): Okochi M., Tsuchiya H., Kumazawa F., Shikida M., Honda H.
  • Organizer: The 14th Symposium of Young Asian Biochemical Engineers' Community
  • Place of Presentation: Shibaura Institute of Technology
  • Year and Date: 2008-11-30
• [Presentation] Magnetic force-based droplet manipulation system for gene expression analysis (2008)
  • Author(s): Mina Okochi, 他
  • Organizer: YABEC2008
  • Place of Presentation: 芝浦工業大学
  • Year and Date: 2008-11-30
• [Presentation] 細胞アレイパターニングを用いた3次元がん細胞挙動評価モデルの構築 (2008)
  • Author(s): 高野翔、大河内美奈、千賀威、本多裕之
  • Organizer: 第39回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2008-11-09
• [Presentation] 磁性微粒子を用いた電気化学検出による小型簡易分析装置の開発 (2008)
  • Author(s): 熊澤史貴、大河内美奈、式田光宏、本多裕之
  • Organizer: 第39回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2008-11-09
• [Presentation] 未修飾磁性微粒子を用いた細胞磁気ラベル法の開発と1細胞機能計測 (2008)
  • Author(s): 小出寛展、伊佐治弥生、大河内美奈、本多裕之
  • Organizer: 第39回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2008-11-09
• [Presentation] 磁性微粒子と磁力を利用した細胞計測技術の開発 (2008)
  • Author(s): 大河内美奈、本多裕之
  • Organizer: 第39回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2008-11-08
• [Presentation] 磁気細胞パターニング法を用いた細胞相互作用解析 (2008)
  • Author(s): 高野翔、伊野浩介、清水一憲、長森英二、大河内美奈、本多裕之
  • Organizer: 化学工学会第40回秋季大会
  • Place of Presentation: 東北大学
  • Year and Date: 2008-09-24
• [Presentation] 未修飾磁性微粒子を用いたL929 磁化細胞アレイの作製とTNF-alphaによる細胞死の検出 (2008)
  • Author(s): 小出寛展、伊佐治弥生、大河内美奈、本多裕之
  • Organizer: 日本生物工学会2008年度大会
  • Place of Presentation: 東北学院大学
  • Year and Date: 2008-08-28
• [Presentation] 未修飾磁性微粒子を用いたL929磁化細胞アレイの作製とTNF-alphaによる細胞死の検出 (2008)
  • Author(s): 小出寛展, 他
  • Organizer: 日本生物工学会2008年度大会
  • Place of Presentation: 東北学院大学
  • Year and Date: 2008-08-28
• [Presentation] Magnetic Force-based lab-on-a-chip for single cell analysis in a droplet. (2008)
  • Author(s): Okochi M, Tsuchiya H., Kumazawa F., Shikida M., Honda H.
  • Organizer: Pacific Rim Meeting on Electrochemical and Solid-state Science 2008
  • Place of Presentation: Honolulu, Hawaii
• [Presentation] Magnetic Force-based lab-on-a-chip for single cell analysis in a droplet (2008)
  • Author(s): Mina Okochi, 他
  • Organizer: Electrochemical Society PACIFIC RIM MEETING
  • Place of Presentation: Hilton Hawaian Village
• [Remarks] ホームページ等
  • URL: http://www.nubio.nagoya-u.ac.jp/proc/index.html
• [Patent(Industrial Property Rights)] がん細胞挙動評価モデル及びその用途 (2008)
  • Inventor(s): 本多裕之、大河内美奈、千賀威、高野翔
  • Industrial Property Rights Holder: 国立大学法人名古屋大学
  • Industrial Property Number: 2008-286173
  • Filing Date: 2008-11-12