MAGNETIC FORCE-BASED-DROPLET-HANDLING SYSTEM FOR SINGLE CELL DIGITAL PCR ANALYSIS
| Project/Area Number |
20360371
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
OKOCHI Mina Nagoya University, 工学研究科, 准教授 (70313301)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HONDA Hiroyuki 名古屋大学, 大学院・工学研究科, 教授 (70209328)
KATO Ryuji 名古屋大学, 大学院・工学研究科, 助教 (50377884)
SHIKIDA Mitsuhiro 名古屋大学, 大学院・工学研究科, 准教授 (80273291)
MIZUTANI Takashi 名古屋大学, 大学院・工学研究科, 教授 (70273290)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2008: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
|
|---|
| Keywords | バイオセンサ / 一細胞解析 / 液滴 / 磁性搬送 / 磁性粒子 / 1細胞解析 / PCR / 遺伝子発現解析 / 細胞 |
|---|
| Research Abstract |
A droplet-based single cell analysis system was developed employing magnetic force-based droplet-handling system. The droplets containing magnetic beads were magnetically controlled and coalesced with another droplet in the oil phase, and all the reactions for RT-PCR including single cell manipulation, cell lysis, cDNA synthesis, and PCR amplification steps were integrated on-chip and applied for detection of cancer cells.
|
Report
(4 results)
Research Products
(61 results)
