Genome-wide analysis and its application in Chinese hamster ovary cells

• Project/Area Number: 20360373
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Osaka University
• Principal Investigator: OMASA Takeshi Osaka University, 大学院・ソシオテクノサイエンス研究部, 教授 (00252586)
• Co-Investigator(Kenkyū-buntansha): SHIRAI Akihiro 徳島大学, 大学院・ソシオテクノサイエンス研究部, 助教 (40380117) ; OHTAKE Hisao 大阪大学, 大学院・工学研究科, 教授 (10127483) ; HONDA Kohsuke 大阪大学, 大学院・工学研究科, 准教授 (90403162)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2008: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: CHO細胞 / ゲノム / BACライブラリー / 染色体 / gene amplification / BAC library / Chinese hamster ovary cell / chromosome

Research Abstract

Chinese hamster ovary (CHO) cells are the most dependable host cells for the industrial production of therapeutic proteins. We constructed CHO genomic bacterial artificial chromosome (BAC) library from a gene-amplified CHO cell line for genome-wide analysis of CHO cell lines. CHO chromosomal markers were constructed by fluorescence in situ hybridization (FISH) imaging using BAC clones as hybridization probes (BAC-FISH). Using constructed tools, we could analyze chromosome rearrangement in CHO cell line.

Research Products

• [Journal Article] Glycosylation pattern of humanized IgG-like bispecific antibody produced by recombinant CHO cells (2010)
  • Author(s): Wook-Dong Kim, Miwako Tokunaga, Hiroyuki Ozaki, Takuya Ishibashi, Kohsuke Honda, Hiroyuki Kajiura, Kazuhito Fujiyama, Ryutaro Asano, Tzumi Kumagai, Takeshi Omasa, Hisao Ohtake
  • Journal Title: Applied Microbiology and Biotechnology Volume 85 Pages: 535-542
  • Peer Reviewed
• [Journal Article] Identification and analysis of specific chromosomal region adjacent to exogenous Dhfr-amplified region in Chinese hamster ovary cell genome (2010)
  • Author(s): Joon Young Park, Yasuhiro Takagi, Miyuki Yamatani, Kohsuke Honda, Shuichi Asakawa, Nobuyoshi Shimizu, Takeshi Omasa, Hisao Ohtake
  • Journal Title: Journal of Bioscience and Bioengineering Vol.109, Number 5 Pages: 504-511
  • NAID: 110007618928
  • Peer Reviewed
• [Journal Article] Effects of palindrome structure on Dhfr amplification in Chinese hamster ovary cells (2010)
  • Author(s): Joon Young Park, Miyuki Yamatani, Souhei Wadano, Yasuhiro Takagi, Kohsuke Honda, Takeshi Omasa, Hisao Ohtake
  • Journal Title: Process Biochemistry Vol.45 Pages: 1845-1851
  • Peer Reviewed
• [Journal Article] Cell engineering and cultivation of Chinese hamster ovary (CHO) cells (2010)
  • Author(s): Takeshi Omasa, Masayoshi Onitsuka, Wook-Dong Kim
  • Journal Title: Current Pharmaceutical Biotechnology vol.11 Pages: 233-240
  • Peer Reviewed
• [Journal Article] Effects of palindrome structure on Dhfr amplification in Chinese hamster ovary cells (2010)
  • Author(s): Joon Young Park, et al.
  • Journal Title: Process Biochemistry Volume: 45 Pages: 1845-1851
  • Peer Reviewed
• [Journal Article] Identification and analysis of specific chromosomal region adjacent to exogenous Dhfr-amplified region in Chinese hamster ovary cell genome (2010)
  • Author(s): Joon Young Park, et al.
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 109 Pages: 504-511
  • Peer Reviewed
• [Journal Article] Glycosylation pattern of humanized IgG-like bispecific antibody produced by recombinant CHO cells (2010)
  • Author(s): Wook-Dong Kim, et al.
  • Journal Title: Applied Microbiology and Biotechnology Volume: 85 Pages: 535-542
  • Peer Reviewed
• [Journal Article] Cell engineering and cultivation of Chinese hamster ovary (CHO) cells (2010)
  • Author(s): Takeshi Omasa, et al.
  • Journal Title: Current Pharmaceutical Biotechnology Volume: 11 Pages: 233-240
  • Peer Reviewed
• [Journal Article] タンパク質医薬品生産宿主としてのChinese hamster ovary(CHO)細胞ゲノム解析-特に染色体の変化について- (2009)
  • Author(s): 曹溢華、大政健史、大竹久夫
  • Journal Title: ソフトウェアバイオロジー 第8巻 Pages: 11-13
• [Journal Article] Bacterial artificial chromosome library for genome-wide analysis of Chinese hamster ovary cells (2009)
  • Author(s): Takeshi Omasa, Yihua Cao,, Joon Young Park, Yasuhiro Takagi, Shuichi Kimura, Hidenori Yano, Kohsuke Honda, Shuichi Asakawa, Nobuyoshi Shimizu, Hisao Ohtake
  • Journal Title: Biotechnology and Bioengineering Volume 104, No.5 Pages: 986-994
  • NAID: 210000003276
  • Peer Reviewed
• [Journal Article] Bacterial artificial chromosome library for genome-wide analysis of Chinese hamster ovary cells (2009)
  • Author(s): Takeshi Omasa, Yihua Cao, Joon Young Park, Yasuhiro Takagi, Shuichi Kimura, Hidenori Yano, Kohsuke Honda, Shuichi Asakawa, Nobuyoshi Shimizu, Hisao Ohtake
  • Journal Title: Biotechnology and Bioengineering 104 Pages: 986-994
  • NAID: 210000003276
  • Peer Reviewed
• [Journal Article] タンパク質医薬品生産宿主としてのChinese hamster ovary(CHO)細胞ゲノム解析-特に染色体の変化について- (2009)
  • Author(s): 曹溢華、大政健史、大竹久夫
  • Journal Title: ソフトウェアバイオロジー 8 Pages: 11-13
• [Journal Article] Chinese hamster ovary (CHO)細胞のBACライブラリー構築とその活用 (2008)
  • Author(s): 大政健史
  • Journal Title: 日本生物工学会誌 vol.86 No.8 Pages: 393-395
• [Journal Article] Chinese hamster ovary (CHO)細胞のゲノム解析とその応用 (2008)
  • Author(s): 大政健史、大竹久夫
  • Journal Title: ソフトウェアバイオロジー 第7巻 Pages: 8-13
• [Journal Article] Chinese hamster ovary (CHO)細胞のBACライブラリー構築とその活用 (2008)
  • Author(s): 大政 健史
  • Journal Title: 日本生物工学会誌 86 Pages: 393-395
• [Journal Article] Chinese hamster ovary (CHO)細胞のゲノム解析とその応用 (2008)
  • Author(s): 大政健史, 大竹久夫
  • Journal Title: ソフトウェアバイオロジー 7 Pages: 8-13
• [Presentation] 細胞内品質管理機構を用いた蛋白質生産向上法の開発 (2011)
  • Author(s): 西沢明敏, 他
  • Organizer: 化学工学会 第76年会
  • Place of Presentation: 東京農工大学, 東京
  • Year and Date: 2011-03-22
• [Presentation] Physical mapping of CHO cell genome (2010)
  • Author(s): Takeshi Omasa
  • Organizer: In ; The 5^<th> International Conference on Geomics (iCG-V), p.57
  • Place of Presentation: Shenzhen, China
  • Year and Date: 2010-11-17
• [Presentation] Physical mapping of CHO cell genome (2010)
  • Author(s): Takeshi Omasa
  • Organizer: The 5^<th> International Conference on Geomics (iCG-V)
  • Place of Presentation: Shenzhen, China
  • Year and Date: 2010-11-17
• [Presentation] Chinese hamster ovary cell genome : impact on cell engineering (2010)
  • Author(s): Takeshi Omasa
  • Organizer: 23^<th> Annual and international meeting of Japanese Association for Animal Cell Technology (JAACT'10)
  • Place of Presentation: Sapporo, Hokkaido
  • Year and Date: 2010-09-01
• [Presentation] Next generation cell engineering for the production of biologics (2010)
  • Author(s): Takeshi Omasa
  • Organizer: In ; the 3^<rd> International Scientific Forum organized by Advanced Medical Research Center, Yokohama City University
  • Place of Presentation: Yokohama, Kanagawa, Japan
  • Year and Date: 2010-02-02
• [Presentation] Next generation cell engineering for the production of biologics (2010)
  • Author(s): Takeshi Omasa
  • Organizer: 3^<rd> International Scientific Forum organized by Advanced Medical Research Center, Yokohama City University
  • Place of Presentation: 横浜市
  • Year and Date: 2010-02-02
• [Presentation] Bacterial artificial chromosome library for genome-wide analysis of Chinese hamster ovary cells (2009)
  • Author(s): Takeshi Omasa, Miyuki Yamatani, Shuiichi Kimura, Yihua Cao, Joon Young Park, Kohsuke Honda, Hisao Ohtake
  • Organizer: In ; 9th Asia-Pacific Biochemical Engineering Conference BP-K2 (AN-010) (APBIOChEC'09) Abstract : Journal of Bioscience and Bioengineering vol.108, issue S1, pages S7-S8 (2009)
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2009-12-26
• [Presentation] Bacterial artificial chromosome library for genome-wide analysis of Chinese hamster ovary cells (2009)
  • Author(s): Takeshi Omasa, Miyuki Yamatani, Shuiichi Kimura, Yihua Cao, Joon Young Park, Kohsuke Honda, Hisao Ohtake
  • Organizer: 9th Asia-Pacific Biochemical Engineering Conference 2009
  • Place of Presentation: 神戸市
  • Year and Date: 2009-11-26
• [Presentation] Production of novel therapeutic antibody in Chinese hamster ovary cells (2009)
  • Author(s): Takeshi Omasa
  • Organizer: In ; International Symposium on Bioprocess and Biosystems Engineering 2009 (ISBBE2009), KIII-1
  • Place of Presentation: Shanghai, China
  • Year and Date: 2009-08-04
• [Presentation] Production of novel therapeutic antibody in Chinese hamster ovary cells (2009)
  • Author(s): Takeshi Omasa
  • Organizer: International Symposium on Bioprocess and Biosystems Engineering 2009
  • Place of Presentation: 中国、上海
  • Year and Date: 2009-08-04
• [Presentation] Chinese Hamster Ovary Cells BAC library : Application to the characterization of CRC chromosomes (2008)
  • Author(s): Takeshi Omasa, Yihua Cao, Shuiichi Kimura, S.M.A. Haghparast, Yasuhiro Takagi, Kohsuke Ronda, Hisao Ohtake
  • Organizer: In ; The thirteenth international biotechnology symposium and exhibition (IBS2008), IL-116, Journal of Biotechnology, vol.136S, p. S141
  • Place of Presentation: Dalian, China
  • Year and Date: 2008-10-16
• [Presentation] Chinese Hamster Ovary Cells BAC library : Application to the characterization of CHO chromosomes (2008)
  • Author(s): T. Omasa, et al.
  • Organizer: IBS2008
  • Place of Presentation: 大連、中華人民共和国
  • Year and Date: 2008-10-16
• [Presentation] Analysis of the specific chromosomal region adjacent to the DHFR gene amplied in CHO DR 1000L-4N cell line (2008)
  • Author(s): T. Omasa, et al.
  • Organizer: Cell Culture Engineering XI
  • Place of Presentation: SunshineCoast, Australia
  • Year and Date: 2008-04-16
• [Presentation] Analysis of the specific chromosomal region adjacent to the DHFR gene amplied in CHO DR 1000L-4N cell line (2008)
  • Author(s): Takeshi Omasa, Yihua Cao, Joon-Young Park, Yasuhiro Takagi, Hidenori Yano, Kohsuke Honda, Shuichi Asakawa, Nobuyoshi Shimizu, Hisao Ohtake
  • Organizer: In ; Engineering Conference International : Cell Culture Engineering XI, 1.B.11, p.105
  • Place of Presentation: Sunshine Coast, Quennsland, Australia
  • Year and Date: 2008-04-14
• [Book] 抗体医薬のための細胞構築と培養技術 (2010)
  • Author(s): 大政健史著・監修
  • Total Pages: 309
  • Publisher: シーエムシー出版
• [Book] 抗体医薬のための細胞構築と培養技術 (2010)
  • Author(s): 大政健史, 他
  • Total Pages: 309
  • Publisher: シーエムシー出版
• [Patent(Industrial Property Rights)] 遺伝子発現安定化エレメント (2010)
  • Inventor(s): 山崎友実、増田兼治、西井重明、川上文清、大政健史
  • Industrial Property Rights Holder: 大阪大学・東洋紡績
  • Acquisition Date: 2010-08-13
• [Patent(Industrial Property Rights)] 遺伝子発現安定化エレメント (2010)
  • Inventor(s): 山崎友実、増田兼治、西井重明、川上文清、大政健史
  • Industrial Property Rights Holder: 大阪大学・東洋紡績
  • Acquisition Date: 2010-08-13