| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2010: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2009: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2008: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Research Abstract |
We studied circadian clocks in cyanobacteria, green algae, and higher plants. 1) in cyanobacteria, we identified the amino acid residue necessary for KaiA-KaiB ; 2) in green algae, we found the interaction between DNA and clock proteins. We examined the complex formation among clock proteins. Additionally, we constructed bioluminescence reporter strains for monitoring nuclear gene expression. 3) We developed an automated, high-throughput, bioluminescence-monitoring and analyzing system. The system is a powerful tool for large-scale screening of mutants followed by large-scale detailed analysis of gene expression.
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