Circadian clock of higher plants, green algae, and cyanobacteria
Project/Area Number |
20370001
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Genetics/Genome dynamics
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Research Institution | Nagoya University |
Principal Investigator |
ISHIURA Masahiro Nagoya University, 遺伝子実験施設, 教授 (20132730)
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Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2010: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2009: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2008: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
|
Keywords | ゲノム機能・発現 / 生物時計 / シロイヌナズナ / 藍色細菌 / クラミドモナス / オルガネラ / 生物発光 / 時計タンパク質 / Kaiタンパク質 / KaiA / KaiB / スピンラベル / 植物時計 / 時計遺伝子 / 進化 |
Research Abstract |
We studied circadian clocks in cyanobacteria, green algae, and higher plants. 1) in cyanobacteria, we identified the amino acid residue necessary for KaiA-KaiB ; 2) in green algae, we found the interaction between DNA and clock proteins. We examined the complex formation among clock proteins. Additionally, we constructed bioluminescence reporter strains for monitoring nuclear gene expression. 3) We developed an automated, high-throughput, bioluminescence-monitoring and analyzing system. The system is a powerful tool for large-scale screening of mutants followed by large-scale detailed analysis of gene expression.
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Report
(4 results)
Research Products
(76 results)
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[Journal Article]2011
Author(s)
Mutoh R, Mino H, Murakami R, Uzumaki T, Ishiura M
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Journal Title
Applied Magnetic Resonance. in press
Related Report
Peer Reviewed
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[Journal Article]2011
Author(s)
Akai M, 小内清, Kusano M, Sato M, Redestig H, Toyooka K, Morishita M, Miyake H, Hazama A, Checchetto V, Szabo I, Matsuoka K, Saito K, Yasui M, Ishiura M, Uozumi N.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article]2011
Author(s)
Matsuo T, Ishiu M
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Journal Title
FEBS lett. 585
Pages: 1495-1502
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Peer Reviewed
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[Journal Article]2010
Author(s)
Matsuo T, Ishiura M
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Journal Title
Int.Rev.Cell Mol.Biol. 280
Pages: 281-314
Related Report
Peer Reviewed
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[Journal Article]2010
Author(s)
Mutoh R, Mino H, Murakami R, Uzumaki T, Takabayashi A, Ishii K, Ishiura M
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Journal Title
Genes Cells. 15
Pages: 269-280
Related Report
Peer Reviewed
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[Journal Article]2010
Author(s)
Kurosawa S, Murakami R, Onai K, Morishita M, Hasegawa D, Iwase R, Uzumaki T, Hayashi F, Kitajima-Ihara T, Sakata S, Murakami M, Kouyama T, Ishiura M
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Journal Title
Genes Cells. 14
Pages: 1-16
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Peer Reviewed
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[Journal Article]2009
Author(s)
Tsunekawa K, Shijuku T, Hayashimoto M, Kojima Y, Onai K, Morishita M, Ishiura M, Kuroda T, Nakamura T, Kobayashi H, Sato M, Toyooka K, Matsuoka K, Omata T, Uozumi N.
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Journal Title
J Biol Chem. 284
Pages: 16513-16521
Related Report
Peer Reviewed
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[Journal Article] Identification and characterization of the Na^-/H^+ antiporter Nhas3 from the thylakoid membrane of Synechocystis sp. PCC 6803.2009
Author(s)
Tsunekawa K, Shijuku T, Hayas himoto M, Kojima Y, Onai K, Morishita M, Ishiura M, Kuroda T, Nakamura T, Kobayashi H, Sato M, Toyooka K, Matsuoka K, Omata T, Uozumi N.
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Journal Title
J Biol Chem. 284(In press)
Related Report
Peer Reviewed
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[Journal Article]2008
Author(s)
Murakami R, Miyake A, Iwase R, Hayashi F, Uzumaki T, Ishiura M
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Journal Title
Genes Cells. 13
Pages: 387-395
Related Report
Peer Reviewed
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[Journal Article]2008
Author(s)
Matsuo T, Okamoto K, 小内清, Niwa Y, Shimogawara K, Ishiura M
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Journal Title
Genes Dev. 22
Pages: 918-930
Related Report
Peer Reviewed
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[Journal Article] Functionally important structural elements of the cyanobacterial clock-related protein Pex.2008
Author(s)
Kurosawa S, Murakami R, Onai K, Morishita M, Hasegawa D, Iwase R, Uzumaki T, Hayashi F, Kitajima-Ihara T, Sakata S, Murakami M, Kouyama T, Ishiura M.
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Journal Title
Genes Cells 14
Pages: 387-395
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Peer Reviewed
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[Presentation] Large scale screening of Arabidopsis mutants in seed oil synthesis by using a high-throughput real-time bioluminescence monitoring system2011
Author(s)
Kawai T, Ona Ki, Hashimoto M, Maeo K, Ishiura M, Nakamura K
Organizer
2nd Gordon Research Conference on Plant Lipids, Structure, Metabolism, Function(GRC on Plant Lipids)
Place of Presentation
Hotel Galvez, Galveston,Texas, USA.(口頭発表)
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