Regulatory mechanisms of meiotic recombination through movements and morphogenesis of chromosomes

• Project/Area Number: 20370003
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Osaka University
• Principal Investigator: SHINOHARA Akira Osaka University, 蛋白質研究所, 教授 (00252578)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2008: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: 減数分裂 / 組換え / 染色体 / 相同組換え / テロメア / 核膜

Research Abstract

Homologous recombination plays a role in chromosome segregation during meiosis as well as production of genome diversity. Meiotic recombination is coupled with the formation of an unique chromosome structure referred as to the Synaptonemal complex. Not only the formation of the unique chromosome structures, but also meiosis shows chromosome motion and rearrangement of chromosomes inside a nucleus. In this study, we showed that the formation of synaptonemal complex and chromosome motion is regulated by phosphorylation of a nuclear envelope protein, Mps3. This suggests the novel role of nuclear envelop in respect of chromosome functions.

Research Products

• [Journal Article] Cyclin-dependent kinase (CDK) promotes formation of the synaptonemal complex in yeast meiosis. (2010)
  • Author(s): Zhu, Z., Mori, D., Oshiumi, H., Matsuzaki, K., Shinohara, M., A. Shinohara
  • Journal Title: Genes-to-Cells. 15 Pages: 1036-1050
  • Peer Reviewed
• [Journal Article] The cyclin-dependent kinase (CDK) promote formation of the synaptonemal complex in yeast meiosis (2010)
  • Author(s): Zhu, Z., Mori, S., Oshiumi, H Matsuzaki, K., Shinohara, M., Shinohara,A.
  • Journal Title: Genes Cells Volume: 15 Pages: 1036-1050
  • Peer Reviewed
• [Journal Article] Genetic Analysis of Baker's Yeast Msh4-Msh5 Reveals a Threshold Crossover Level for Meiotic Viability (2010)
  • Author(s): Nishant, K.T., C.Chen, M.Shinohara, A.Shinohara, E.Alani
  • Journal Title: PLoS Genet. Volume: 6
  • Peer Reviewed
• [Journal Article] Collaborative Action of Brcal and CtlP in Elimination of Covalent Modifications from Double-Strand Breaks to Facilitate Subsequent Break Repair. (2010)
  • Author(s): Nakamura K, Kogame T, Oshiumi H, Shinohara A, Sumitomo Y, Agama K, Pommier Y, Tsutsui KM, Tsutsui K, Hartsuiker E, Ogi T, Takeda S., Taniquchi Y.
  • Journal Title: PLoS Genetics 4
  • Peer Reviewed
• [Journal Article] 配偶子の染色体の数を保証する蛋白質 (2009)
  • Author(s): 篠原彰
  • Journal Title: 化学と生物 47 Pages: 6-8
  • Peer Reviewed
• [Journal Article] 染色体組換えの制御機構概論 (2009)
  • Author(s): 篠原彰
  • Journal Title: 蛋白質核酸酵素 54 Pages: 447-449
  • Peer Reviewed
• [Journal Article] 配偶子の染色体の数を保証する蛋白質 (2009)
  • Author(s): 篠原彰
  • Journal Title: 蛋白質核酸酵素 54 Pages: 466-471
  • Peer Reviewed
• [Journal Article] Rad52 promotes post-invasion steps of meiotic double-strand-break-repair. (2008)
  • Author(s): Luo, J.P., Oh, S.D., Shinohara, M., Shinohara, A., Hunter, N.
  • Journal Title: Mol.Cell. 29 Pages: 517-552
  • Peer Reviewed
• [Journal Article] Crossover assurance and crossover interference are distinctly regulated by the ZMM proteins during yeast meiosis. (2008)
  • Author(s): Shinohara, M., Oh, S.D., Hunter, N., Shinohara, A.
  • Journal Title: Nature Genet. 40 Pages: 299-309
  • Peer Reviewed
• [Journal Article] Shinohara. FHA domain of yeast Xrs2, a homologue of human Nbs1, promotes non-homologous end joining through the interaction with a Ligase IV partner protein (2008)
  • Author(s): Matsuzaki, K., Shinohara, A., M. Shinohara
  • Journal Title: Lif1.Genetics. 179 Pages: 213-225
  • Peer Reviewed
• [Journal Article] Telomeres drive rapid movements regulated by chromosome status in meiotic prophase. (2008)
  • Author(s): Conrad, M.N., Lee, C.-Y., Chao, G., Shinohara, M., Kosaka, H., Shinohara, A., Conchello, J.-A., M. E. Dresser
  • Journal Title: Cell. 133 Pages: 1175-1187
  • Peer Reviewed
• [Journal Article] Shinohara. Csm4-dependent telomere movement on nuclear envelope promotes meiotic recombination. (2008)
  • Author(s): Kosaka, H., Shinohara, M., A. Conchello
  • Journal Title: PLoS Genetics. 4
  • Peer Reviewed
• [Journal Article] FHA domain of yeast Xrs2, a homologue of human Nbs1, promotesnon-homologous end joining through the interaction with a Ligase IV partner protein, Lif1. (2008)
  • Author(s): Matsuzaki, K., Shinohara, A., M. Shinohara.
  • Journal Title: Genetics 179 Pages: 213-225
  • Peer Reviewed
• [Journal Article] Telomeres drive rapid movements regulated by chromosome status in meiotic prophase. (2008)
  • Author(s): Conrad, M. N., Lee, C. -Y., Chao, G., Shinohara, M, Kosaka, H., Shinohara, A., Conchello, J. -A., M. E. Dresser.
  • Journal Title: Cell 133 Pages: 1175-1187
  • Peer Reviewed
• [Journal Article] Csm4-dependent telomere movement on nuclear envelope promotes meiotic recombination. (2008)
  • Author(s): Kosaka, H., Shinohara, M., A. Shinohara.
  • Journal Title: PLoS Genetics 4
  • Peer Reviewed
• [Journal Article] 配偶子の染色体異常をふせぐタンパク質複合体のはたらき. (2008)
  • Author(s): 篠原彰
  • Journal Title: メディカルバイオ 5 Pages: 12-13
• [Journal Article] 減数分裂期のDNA交換反応と染色体の形態形成のしくみ. (2008)
  • Author(s): 篠原彰, 篠原美紀
  • Journal Title: 蛋白質核酸酵素 53 Pages: 1315-13256
  • NAID: 40016204743
  • Peer Reviewed
• [Presentation] The PCSS (Psy3-Csm2-Shu1-Shu2) complex, a new Rad51 mediator in homologous recombination. (2011)
  • Author(s): A. Shinohara
  • Organizer: Keystone Symposia on DNA replication and recombination.
  • Place of Presentation: Keystone, CO, USA
• [Presentation] The PCSS (Psy3-Csm2-Shu1-Shu2) complex, a new Rad51 mediator in homologous recombination. (2011)
  • Author(s): Akira Shinohara
  • Organizer: Keystone Symposia on DNA Replication and Recombination
  • Place of Presentation: Keystone, USA
• [Presentation] The PCSS(Psy3-Csm2-Shul-Shu2) complex, a new Rad51 mediator in homologous recombination (2011)
  • Author(s): 篠原彰
  • Organizer: Keystone Symposia on DNA replication and recombination
  • Place of Presentation: Keystone, CO, USA(招待演講)
• [Presentation] CDK (cyclin-dependent kinase)- and DDK (Dbf4-dependent kinase)-dependent regulation of chromosome movements in meiosis. (2010)
  • Author(s): A. Shinohara
  • Organizer: GRC meeting on Meiosis New London
  • Place of Presentation: NH, USA
• [Presentation] The PCSS (Psy3-Csm2-Shu1-Shu2) complex, a new Rad51 mediator in homologous recombination. (2010)
  • Author(s): A.Shinohara
  • Organizer: EMBO workshop on recombination and connection to sumo and ubiqutin module.
  • Place of Presentation: Il Cicco, Italy
• [Presentation] CDK (cyclin-dependent kinase)- and DDK (Dbf4-dependent kinase)-dependent regulation of chromosome movements in meiosis. (2010)
  • Author(s): Akira Shinohara
  • Organizer: Gorden Research Conference on Meiosis
  • Place of Presentation: New London, USA
• [Presentation] The PCSS (Psy3-Csm2-Shu1-Shu2) complex, a new Rad51 mediator in homologous recombination. (2010)
  • Author(s): Akira Shinohara
  • Organizer: EMBO workshop on recombination mechanisms
  • Place of Presentation: Lucca, Italy
• [Presentation] The PCSS(Psy3-Csm2-Shu1-Shu2) complex plays a role in Rad51-assembly in homologous recombination (2010)
  • Author(s): 篠原彰
  • Organizer: EMBO workshop on recombination and connection to sumo and ubiqutin module
  • Place of Presentation: Il Cicco, Italy(招待演講)
• [Presentation] CDK(cyclin-dependent kinase)- and DDK(Dbf4-dependent kinase)-dependent regulation of chromosome movements in meiosis (2010)
  • Author(s): 篠原彰
  • Organizer: GRC meeting on Meiosis
  • Place of Presentation: New London, NH, USA(招待演講)
• [Presentation] Psy3-Csm2-Shu1-Shu2complex is a new Rad51 mediator in homologous recombination. (2009)
  • Author(s): A. Shinohara
  • Organizer: FASEB meeting on recombination ang genome rearrangement
  • Place of Presentation: Snowmass, USA
• [Presentation] CDK (cyclin-dependent kinase)- and DDK (Dbf4-dependent kinase)-dependent regulation of chromosome movements in meiosis. (2009)
  • Author(s): A. Shinohara
  • Organizer: Cantablanco meeting on Genome stability
  • Place of Presentation: Madrid, Spain
• [Presentation] CDK (cyclin-dependent kinase)- and DDK (Dbf4-dependent kinase)-dependent regulation of chromosome movements in meiosis. (2009)
  • Author(s): Akira Shinohara
  • Organizer: EMBO workshop on meiosis
  • Place of Presentation: Lsle sure la Sorgue, France
• [Presentation] CDK (cyclin-dependent kinase)- and DDK (Dbf4-dependent kinase)-dependent regulation of chromosome movements in meiosis. (2009)
  • Author(s): Akira Shinohara
  • Organizer: Late Summer meeting on Chromosome Dynamics
  • Place of Presentation: IMP Vienna, Austria
• [Presentation] A new protein complex for assembly of Rad51. (2009)
  • Author(s): Akira Shinohara
  • Organizer: FASEB conference on genetic recombination & genome rearrangement
  • Place of Presentation: Snowmass USA
• [Presentation] Cdc53 promotes the formation of the synatponemal complex during meiosis. (2009)
  • Author(s): Akira Shinohara
  • Organizer: Gorden Research Conference on Meiosis
  • Place of Presentation: New London, USA
• [Presentation] Regulation of Chromosome dynamics by cell cycle kinaases. (2009)
  • Author(s): Akira. Shinohara
  • Organizer: EMBO workshop on recombination and connection to sumo and ubiqutin module.
  • Place of Presentation: Il Cicco, Italy
• [Presentation] Two cell cycle kinases, CDK (cyclin-dependent kinase) and DDK (Dbf4-dependent kinase), control chromosome dynamics during meiosis (2009)
  • Author(s): 篠原彰
  • Organizer: Contoblanco workshop on Biology, Molecular Mechanisms of Genome stability
  • Place of Presentation: Madrid Spain
• [Presentation] In vivo and in vitro characterization of the Psy3-Csm2-Shu1-Shu2 (PCSS) complex, a new Rad51 mediator, in recombination (2009)
  • Author(s): 篠原彰
  • Organizer: FASEB Conference on genetic recombination and gennme rearrangement
  • Place of Presentation: Snowmass USA
• [Presentation] Two cell cycle kinases, CDK (cyclin-dependent kinase) and DDK (Dbf4-dependent kinase). control chromosome dynamics durina meiosis (2009)
  • Author(s): 篠原彰
  • Organizer: Late Summer meeting on Chromosome Dynamics
  • Place of Presentation: Vienna, Austria
• [Presentation] Two cell cycle kinases, CDK (cyclin-dependent kinase) and DDK (Dbf4-dependent kinase), control chromosome dynamics during meiosis (2009)
  • Author(s): 篠原彰
  • Organizer: EMBO Workshop on meiosis
  • Place of Presentation: Lsle sure la Sorgue, France
• [Presentation] Control of synaptonemal complex formation by SCF ubiquitin ligase complex. (2008)
  • Author(s): A. Shinohara
  • Organizer: GRC meeting on Meiosis
  • Place of Presentation: New Hampshere USA
• [Presentation] Regulation of Chromosome dynamics by cell cycle kinaases.Contoblanco workshop on Biology (2008)
  • Author(s): Akira Shinohara
  • Organizer: Molecular Mechanisms of Genome stability
  • Place of Presentation: Madrid, Spain
• [Presentation] Chromosome morphogenesis during meiosis (2008)
  • Author(s): 篠原 彰
  • Organizer: Nuclear dynamics meeting
  • Place of Presentation: 伊勢
• [Presentation] Control of synaptonemal complex formation by SCF ubiquitin ligase complex (2008)
  • Author(s): 篠原 彰
  • Organizer: EMBO workshop on recombination
  • Place of Presentation: Ciocco, Italy
• [Presentation] Control of synaptonemal complex formation by SCF ubiquitin ligase complex (2008)
  • Author(s): 篠原 彰
  • Organizer: GRC meeting on Meiosis
  • Place of Presentation: New Hampshere USA
• [Presentation] Does mutations occur more frequently in meiosis than in mitosis? (2008)
  • Author(s): 篠原 彰
  • Organizer: The 3R meeting
  • Place of Presentation: 嬬恋
• [Presentation] 減数分裂期特異的染色体構造体-シナプトネマ複合体-の形成制御 (2008)
  • Author(s): 篠原 彰
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 神戸
• [Book] 生物学辞典 (2011)
  • Author(s): 山本正幸, ら編
  • Publisher: 分担執筆
• [Remarks] ホームページ等
  • URL: http://www.protein.osaka-u.ac.jp/genome/Shinohara-HP-index.html
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/genome/Shinohara-HP-index.html
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/genome/Shinohara-HP-index.html
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/genome/Shinohara-HP-index.html