Analysis of the molecular mechanism of the initiation of chromosomal DNA replication in eukaryotes

• Project/Area Number: 20370005
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: National Institute of Genetics
• Principal Investigator: TANAKA Seiji 国立遺伝学研究所, 細胞遺伝研究系, 助教 (50263314)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
• Keywords: DNA複製 / CDK / 細胞周期 / 複製開始 / DDK

Research Abstract

Chromosomal DNA replication in eukaryotes initiates from multiple origins of replication. Because chromosomal DNA must be precisely duplicated per every cell cycle, cells have regulatory mechanisms that limit the origin activation to once per cell cycle. In this study, we found how cells inhibit the premature untimely initiation of DNA replication and how to coordinate the activation of multiple replication origins.

Research Products

• [Journal Article] Origin association of Sld3, Sld7, and Cdc45 proteins is a key step for determination of origin-firing timing (2011)
  • Author(s): Tanaka, S., Nakano, R., Katou, Y., Shirahige, K. and Araki, H.
  • Journal Title: Curr. Biol Volume: 21 Issue: 24 Pages: 2055-2063
  • DOI: 10.1016/j.cub.2011.11.038
  • Peer Reviewed
• [Journal Article] Multiple regulatory mechanisms to inhibit untimely initiation of DNA replication are important for stable genome maintenance (2011)
  • Author(s): Tanaka, S. and Araki, H
  • Journal Title: PLoS Genet Volume: 7 Issue: 6 Pages: e1002136-e1002136
  • DOI: 10.1371/journal.pgen.1002136
  • Peer Reviewed
• [Journal Article] Multiple Regulatory Mechanisms to Inhibit Untimely Initiation of DNA Replication Are Important for Stable Genome Maintenance. (2011)
  • Author(s): Tanaka, S., Araki, H.
  • Journal Title: PLoS Genetics in press.
  • Peer Reviewed
• [Journal Article] Multiple Regulatory Mechanisms to Inhibit Untimely Initiation of DNA Replication Are Important for Stable Genome Maintenance. (2011)
  • Author(s): Tanaka, S.
  • Journal Title: PLoS Genetics Volume: 7(In press)
  • Peer Reviewed
• [Journal Article] Regulation of the initiation step of DNA replication by cyclin-dependent kinases (2010)
  • Author(s): Tanaka, S. and Araki, H
  • Journal Title: Chromosoma Volume: 119 Issue: 6 Pages: 565-574
  • DOI: 10.1007/s00412-010-0291-8
  • Peer Reviewed
• [Journal Article] Regulation of the initiation step of DNA replication by cyclin-dependent kinases. (2010)
  • Author(s): Tanaka, S., Araki, H.
  • Journal Title: Chromoosma 119 Pages: 565-574
  • Peer Reviewed
• [Journal Article] Regulation of the initiation step of DNA replication by cyclin-dependent kinases. (2010)
  • Author(s): Tanaka, S.
  • Journal Title: Chromosoma Volume: 119 Pages: 565-574
  • Peer Reviewed
• [Journal Article] 真核細胞の細胞周期制御機構と染色体DNA複製 (2009)
  • Author(s): 田中誠司、荒木弘之
  • Journal Title: 遺伝 63 Pages: 51-58
• [Journal Article] 真核細胞の細胞周期制御機構と染色体DNA複製 (2009)
  • Author(s): 田中誠司
  • Journal Title: 遺伝 63 Pages: 51-58
• [Journal Article] 真核細胞染色体DNA複製開始反応とCDKによる制御 (2008)
  • Author(s): 田中誠司、荒木弘之
  • Journal Title: 細胞工学 27 Pages: 985-991
• [Journal Article] 真核細胞染色体DNA複製開始反応とCDKによる制御. (2008)
  • Author(s): 田中誠司
  • Journal Title: 細胞工学 27 Pages: 985-991
• [Presentation] Origin Association of Sld3, Sld7, and Cdc45 Proteins is a Key Step for Determination of Origin-Firing Timing (2011)
  • Author(s): Seiji Tanaka
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市(招待講演)
  • Year and Date: 2011-12-16
• [Presentation] DDK-dependent recruitment of Sld3, Sld7 and Cdc45 specifies the timing of origin-firing in the budding yeast Saccharomyces cerevisiae (2011)
  • Author(s): 田中誠司
  • Organizer: 第21回DNA複製・組換え・ゲノム安定性制御ワークショップ
  • Place of Presentation: 福岡県福津市
  • Year and Date: 2011-10-26
• [Presentation] DDK-dependent recruitment of Sld3, Sld7 and Cdc45 specifies the timing of origin-firing in budding yeast (2011)
  • Author(s): Seiji Tanaka
  • Organizer: Cold Spring Harbor Laboratory Meeting Eukaryotic DNA replication and genome maintenance
  • Place of Presentation: Cold Spring Harbor, NY, USA
  • Year and Date: 2011-09-10
• [Presentation] DDK-dependent recruitment of Sld3, Sld7 and Cdc45 specifies the timing of origin-firing in budding yeast (2011)
  • Author(s): Tanaka, S.
  • Organizer: 2011 Cold Sprong Harbor Laboratory Meeting on EUKARYOTIC DNA REPLICATION & GENOME MAINTENANCE
  • Place of Presentation: Cold Spring Harbor, NY, USA
  • Year and Date: 2011-09-09
• [Presentation] DDK-dependent recruitment of Sld3, Sld7 and Cdc45 specifies the timing of origin-firing in the budding yeast Saccharomyces cerevisiae (2011)
  • Author(s): 田中誠司
  • Organizer: 2011年関東東海DNA研究会
  • Place of Presentation: 長野県山形村
  • Year and Date: 2011-07-18
• [Presentation] Yeast initiation proteins, Sld3, Sld7 and Cdc45, specifies the timing of origin-firing (2011)
  • Author(s): Seiji Tanaka
  • Organizer: 6th UK-Japan Cell Cycle Workshop
  • Place of Presentation: Lake Windermere, UK
  • Year and Date: 2011-04-12
• [Presentation] Yeast initiation proteins, Slds3, Sldi7 and Cdc45, specifies the timing of origin-firing (2011)
  • Author(s): Tanaka, S.
  • Organizer: 6th UK-Japan Cell Cycle Workshop
  • Place of Presentation: Lake Windermere, United Kingdom
  • Year and Date: 2011-04-02
• [Presentation] CDKに依存した染色体DNA複製開始の分子機構 (2010)
  • Author(s): 荒木弘之
  • Organizer: 第33回 日本分子生物学会年会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-09
• [Presentation] Temporal regulation of activation of replication origins in the budding yeast Saccharomyces cerevisiae. (2010)
  • Author(s): 田中誠司
  • Organizer: 第33回 日本分子生物学会年会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-08
• [Presentation] Cdc7-Dbf4 kinase specifies early replication origins by regulating the association of low-abundant replication initiation factors in budding yeast. (2010)
  • Author(s): Seiji Tanaka
  • Organizer: 2010 3R (Replication, Recombination & Repair) Symposium
  • Place of Presentation: 富山市
  • Year and Date: 2010-10-28
• [Presentation] 真核生物におけるDNA複製制御機構とゲノム安定維持・高次複製制御機構 (2010)
  • Author(s): 田中誠司
  • Organizer: 酵母遺伝学フォーラム第43回研究報告会
  • Place of Presentation: 奈良市
  • Year and Date: 2010-09-11
• [Presentation] DNA複製制御機構とゲノム安定維持・高次複製制御機構 (2010)
  • Author(s): 田中誠司
  • Organizer: 2010年 関東東海DNA研究会
  • Place of Presentation: 神奈川県箱根町
  • Year and Date: 2010-09-06
• [Presentation] Howearly firingg origins are determined in budding yeast (2010)
  • Author(s): Tanaka, S.
  • Organizer: FASEB Summer Research Conference 'Yeast Chromosome Structure, Replication & Segregation'
  • Place of Presentation: Carefree, AZ, USA
  • Year and Date: 2010-08-11
• [Presentation] How early-firing origins are determined in budding yeast. (2010)
  • Author(s): Seiji Tanaka
  • Organizer: 2010 FASEB Summer Research Conferences "Yeast Chromosome Structure, Replication & Segregation"
  • Place of Presentation: Carefree, Arizona, USA
  • Year and Date: 2010-08-11
• [Presentation] Multiple regulatory mechanisms of the initiation of DNA replication are important for stable genome maintenance (2010)
  • Author(s): 田中誠司
  • Organizer: International Conference on Radiation and Cancer Biology at Nagasaki 2010
  • Place of Presentation: 長崎市
  • Year and Date: 2010-02-18
• [Presentation] 真核生物における染色体DNA複製開始の制御機構 (2010)
  • Author(s): 荒木弘之
  • Organizer: 生命活動を制御する高次複合体の構造と機能
  • Place of Presentation: 福岡市
  • Year and Date: 2010-02-17
• [Presentation] How early-firing origins are determined in budding yeast. (2010)
  • Author(s): Tanaka, S., Araki, H.
  • Organizer: 2010 FASEB Summer Research Conferences "Yeast Chromosome Structure, Replication & Segregation"
  • Place of Presentation: at Carefree, AZ.USA.
• [Presentation] Cdc7-Dbf4 kinase specifies early replication origins by regulating the association of low-abundant replication initiation factors in budding yeast. (2010)
  • Author(s): Tanaka, S.
  • Organizer: 2010 3R (Replication, Recombination & Repair) Symposium
  • Place of Presentation: at Toyama.
• [Presentation] Multiple regulatory mechanisms of the initiation of DNA replication are important for stable genome maintenance. (2010)
  • Author(s): Tanaka, S.
  • Organizer: International Conference on Radiation and Cancer Biology at Nagasaki 2010
  • Place of Presentation: at Nagasaki.
• [Presentation] Temporal regulation of origin firing in budding yeast Saccharomyces cerevisiae (2009)
  • Author(s): 田中誠司
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-12-10
• [Presentation] Multiple regulatory mechanisms of the initiation of DNA replication are important for stable genome maintenance (2009)
  • Author(s): 田中誠司
  • Organizer: 2009年度複製・組換え・修復ワークショップ
  • Place of Presentation: 彦根市
  • Year and Date: 2009-11-02
• [Presentation] Multiple regulatory mechanisms of the initiation of DNA replication are important for stable genome maintenance (2009)
  • Author(s): Tanaka, S.
  • Organizer: Cold Spring Harbor Meeting "Eukaryotic DNA Replication & Genome Maintenance"
  • Place of Presentation: Cold Spring Harbor, NY, USA
  • Year and Date: 2009-09-02
• [Presentation] Multiple regulatory mechanisms of the initiation of DNA replication are important for stable genome maintenance (2009)
  • Author(s): 田中誠司
  • Organizer: Cold Spring Harbor Meeting, Eukaryotic DNA Replication & Genome Maintenance
  • Place of Presentation: NY, USA
  • Year and Date: 2009-09-02
• [Presentation] CDK-dependent assembly of replication proteins at the initiation step of chromosomal DNA replication (2009)
  • Author(s): 荒木弘之
  • Organizer: Cold Spring Harbor Meeting, Eukaryotic DNA Renlication & Genome Maintenance
  • Place of Presentation: NY, USA
  • Year and Date: 2009-09-01
• [Presentation] DNA複製開始制御システムとゲノム安定維持 (2009)
  • Author(s): 田中誠司
  • Organizer: 酵母遺伝学フォーラム第42回研究報告会
  • Place of Presentation: つくば市
  • Year and Date: 2009-07-28
• [Presentation] Multiple regulatory mechanisms for the initiation of DNA replication are important for stable genome maintenance (2009)
  • Author(s): 田中誠司
  • Organizer: 関東東海DNA研究会
  • Place of Presentation: 箱根町
  • Year and Date: 2009-07-23
• [Presentation] Multiple regulatory mechanisms of the initiation of DNA replication are important for stable genome maintenance (2009)
  • Author(s): Tanaka, S.
  • Organizer: Swiss-Japan Cell Cycle Meeting-Chromosome Dynamics and Genome Stability
  • Place of Presentation: Villars-sur-Ollons, Switzerland
  • Year and Date: 2009-05-15
• [Presentation] Multiple regulatory mechanisms for the initiation of DNA replication are important for stable genome maintenance (2009)
  • Author(s): 田中誠司
  • Organizer: Chromosome Dynamics and Genome Stability
  • Place of Presentation: Villars-sur-Ollons, Switzer land
  • Year and Date: 2009-05-15
• [Presentation] Multiple regulatory mechanisms of the initiation of DNA replication are important for stable genome maintenance. (2009)
  • Author(s): Tanaka, S., Araki, H.
  • Organizer: Cold Spring Harbor Meeting "Eukaryotic DNA Replication & Genome Maintenance"
  • Place of Presentation: at Cold Spring Harbor, NY.USA.
• [Presentation] Regulation of the replication protein Sld2 and its role in stable genome maintenance. (2008)
  • Author(s): Tanaka, S.
  • Organizer: BMB2008
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-10
• [Presentation] CDK-dependent assembly of replication protein to initiate chromosomal DNA replication (2008)
  • Author(s): Araki, H.
  • Organizer: BMB2008
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-10
• [Presentation] Periodic expression of replication protein Sld2 is important for DNA replication and genome integrity (2008)
  • Author(s): Tanaka, S.
  • Organizer: 3R Symposium 2008
  • Place of Presentation: 静岡県掛川市
  • Year and Date: 2008-10-27
• [Presentation] サイクリン依存性キナーゼによる染色体DNAの複製制御機構 (2008)
  • Author(s): 田中誠司
  • Organizer: JST終了シンポジウム
  • Place of Presentation: 東京都
  • Year and Date: 2008-10-22
• [Presentation] Regulation of the replication protein Sld2 and its role in stable genome maintenance (2008)
  • Author(s): Tanaka, S.
  • Organizer: 酵母遺伝学フォーラム第41回研究報告会
  • Place of Presentation: 札幌市
  • Year and Date: 2008-09-10
• [Presentation] Regulation of the replication protein Sld2 and its role in stable genome maintenance (2008)
  • Author(s): Tanaka, S.
  • Organizer: 2008年中部東海DNA研究会
  • Place of Presentation: 神奈川県箱根町
  • Year and Date: 2008-08-11
• [Presentation] CDK-dependent Assembly of Replication Proteins to Initiate Chromosomal DNA Replication (2008)
  • Author(s): Araki, H.
  • Organizer: DNA REPLICATION and GENOME INTEGRITY2008
  • Place of Presentation: CA, USA
  • Year and Date: 2008-07-19
• [Presentation] Molecular Mechanism of the Initiation Step of Chromosomal DNA Replication in Budding Yeast (2008)
  • Author(s): Araki, H.
  • Organizer: XX International Congress of Genetics 2008
  • Place of Presentation: Belrin, Germany
  • Year and Date: 2008-07-13
• [Presentation] Periodic expression of Sld2 is important for DNA replication and genome integrity (2008)
  • Author(s): Tanaka, S.
  • Organizer: FASEB Summer Research Conference 'Yeast Chromosome Structure, Replication & Segregation'
  • Place of Presentation: Carefree, AZ, USA
  • Year and Date: 2008-06-25
• [Presentation] Periodic expression of Sld2 is important for DNA replication and genome integrity (2008)
  • Author(s): Tanaka, S.
  • Organizer: FASEB「Yeast Chromosome Structure, Replication & Segregation」
  • Place of Presentation: AZ, USA
  • Year and Date: 2008-06-25
• [Presentation] REGULATION OF THE REPLICATION PROTEIN Sld2 AND ITS ROLE IN STABLE GENOME MAINTENANCE (2008)
  • Author(s): Tanaka, S.
  • Organizer: The 3rd NIG International Symposium "Chromosome Dynamics"
  • Place of Presentation: 静岡県三島市
  • Year and Date: 2008-05-26
• [Presentation] CDK-dependent initiation of chromosomal DNA replication in budding yeast. (2008)
  • Author(s): Araki, H.
  • Organizer: Cold spring Harbor Laboratory Meeting "The Cell Cycle"
  • Place of Presentation: NY, USA
  • Year and Date: 2008-05-15
• [Presentation] Periodic expression of Sld2 is important for DNA replication and genome integrity. (2008)
  • Author(s): Tanaka, S., Araki, H.
  • Organizer: 2008 FASEB Summer Research Conferences "Yeast Chromosome Structure, Replication & Segregation"
  • Place of Presentation: at Carefree, AZ.USA.
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