Molecular Mechanism of Nascent Peptide-Mediated Translation Arrest Involved in Feedback Regulation of Methionine Biosynthesis
| Project/Area Number |
20370016
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Hokkaido University |
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Principal Investigator |
NAITO Satoshi Hokkaido University, 大学院・農学研究院, 教授 (20164105)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2008: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | シロイヌナズナ / メチオニン / 翻訳制御 / フィードバック制御 / 新生ペプチド / 試験管内翻訳系 / フィードバック / ペプチド |
|---|
| Research Abstract |
Cystathionine γ-synthase (CGS) catalyzes the first committed step of methionine biosynthesis in plants, and is encoded by CGS1 gene in Arabidopsis. Expression of CGS1 is feedback-regulated at the step of CGS1 mRNA degradation and S-adenosyl-L-methionine (SAM) acts as an effector. We found that, when ribosome translates the CGS1 mRNA in the presence of SAM, either the ribosome itself or the nascent peptide within the ribosomal exit tunnel changes its conformation. We also obtained evidence suggesting that the ribosomal proteins that constitute the exit tunnel is involved in this regulation.
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Report
(4 results)
Research Products
(30 results)
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[Presentation] トンネルの闇の中から:植物におけるメチオニン生合成のフィードバック制御機構2010
Author(s)
山下由衣, 尾上典之, 室田勝功, 小松陽平, 西口達也, 竹原好美, 山下(永見)陽子, 原口雄飛, 尾之内均, 内藤哲
Organizer
第10回日本蛋白質科学会年会,ノーベル賞特別シンポジウム「タンパク質合成装置リボソームの最新像」
Place of Presentation
札幌,札幌コンベンションセンター 招待講演
Year and Date
2010-06-18
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