| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2008: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Research Abstract |
Cystathionine γ-synthase (CGS) catalyzes the first committed step of methionine biosynthesis in plants, and is encoded by CGS1 gene in Arabidopsis. Expression of CGS1 is feedback-regulated at the step of CGS1 mRNA degradation and S-adenosyl-L-methionine (SAM) acts as an effector. We found that, when ribosome translates the CGS1 mRNA in the presence of SAM, either the ribosome itself or the nascent peptide within the ribosomal exit tunnel changes its conformation. We also obtained evidence suggesting that the ribosomal proteins that constitute the exit tunnel is involved in this regulation.
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