Elucidation of molecular mechanisms underlying the early signaling of nodulation using Lotus japonicus

• Project/Area Number: 20370020
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: National Institute for Basic Biology (2009-2011); The University of Tokyo (2008)
• Principal Investigator: KAWAGUCHI Masayoshi 基礎生物学研究所, 共生システム研究部門, 教授 (30260508)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2008: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
• Keywords: 植物微生物相互作用・共生 / シグナル伝達 / ミヤコグサ / 根粒形成 / Nod factor / 共生 / 根粒菌 / Nodファクター / 発生

Research Abstract

Nod factors secreted from rhizobia lead to the formation of novel organs, termed nodules. We focused on Lotus japonicus NSP1 and NSP2, the components of nod factor signaling pathway, and analyzed their actions in relation to HAR1-mediated systemic regulation of nodulation. Then we identified a nucleoporin Seh1 required for nodulation and arbuscular mycorrhization, and elucidated its physical interaction with NUP85.Furthermore, we identified a novel regulator TML that functions in the roots downstream of HAR1.

Research Products

• [Journal Article] The Intergral Membrane Protein SEN1 is Required for Symbiotic Nitrogen Fixation in Lotus japonicus Nodules (2012)
  • Author(s): Hakoyama T, Niimi K, et al
  • Journal Title: Plant and Cell Physiology Volume: 53 Pages: 225-36
  • Peer Reviewed
• [Journal Article] Expression and functional analysis of a CLV3-like gene in the model legume Lotus japonicus (2012)
  • Author(s): Okamoto S, et al
  • Journal Title: Plant and Cell Physiology Volume: 51 Pages: 1211-21
  • Peer Reviewed
• [Journal Article] The evolution of symbiotic systems (2011)
  • Author(s): Kawaguchi M
  • Journal Title: Cellular and Molecular Life Sciences Volume: 68 Pages: 1283-4
• [Journal Article] The Clavata2 genes of pea and Lotus japonicus affect autoregulation of nodulation. (2011)
  • Author(s): Krusell L, Sato N, et al.
  • Journal Title: Plant Journal in press. Volume: 65 Pages: 861-871
  • Peer Reviewed
• [Journal Article] The Clavata2 genes of pea and Lotus japonicus affect autoregulation of nodulation. (2011)
  • Author(s): Krusell L, Sato N, et al.
  • Journal Title: Plant Journal Volume: 65 Pages: 861-871
  • Peer Reviewed
• [Journal Article] NENA, a Lotus japonicus homolog of Sec13, is required for rhizodermal infection by arbuscularmycorrhiza fungi and rhizobia but dispensable for cortical endosymbiotic development (2010)
  • Author(s): Groth, M., Takeda, N.,(他8名, 8番目)
  • Journal Title: Plant Cell Volume: 22 Pages: 2509-26
  • Peer Reviewed
• [Journal Article] How many peas in a pod? Legume genes responsible for mutualistic symbioses underground (2010)
  • Author(s): Kouchi, H., Imaizumi-Anraku, H., Hayashi, M., Hakoyama, T., Nakagawa, T., Umehara, Y., Suganuma, N., Kawaguchi, M
  • Journal Title: Plant Cell Physiol Volume: 51 Pages: 1381-97
  • NAID: 10027351824
  • Peer Reviewed
• [Journal Article] NENA, a Lotus japonicus homolog of Sec13, is required for rhizodermal infection by arbuscularmycorrhiza fungi and rhizobia but dispensable for cortical endosymbiotic development. (2010)
  • Author(s): Groth, M., Takeda, N., 他8名
  • Journal Title: Plant Cell 22 Pages: 2509-2526
  • Peer Reviewed
• [Journal Article] How many peas in a pod? Legume genes responsible for mutualistic symbioses underground. (2010)
  • Author(s): Kouchi, H., Imaizumi-Anraku, H., Hayashi, M., Hakoyama, T., Nakagawa, T., Umehara, Y., Suganuma, N., Kawaguchi, M.
  • Journal Title: Plant Cell Physiol. 51 Pages: 1381-1397
  • NAID: 10027351824
  • Peer Reviewed
• [Journal Article] A receptor-like kinase, KLAVIER, mediates systemic regulation of nodulation and non-symbiotic shoot development in Lotus japonicus. (2010)
  • Author(s): Miyazawa H, et al.
  • Journal Title: Development Volume: 137 Pages: 4317-25
  • Peer Reviewed
• [Journal Article] How many peas in a pod? Legume genes responsible for mutualistic symbioses underground (2010)
  • Author(s): Kouchi H, et al.
  • Journal Title: Plant Cell Physiol. Volume: 51 Pages: 1381-97
  • Peer Reviewed
• [Journal Article] A receptor-like kinase, KLAVIER, mediates systemic regulation of nodulation and non-symbiotic shoot development in Lotus japonicus. (2010)
  • Author(s): Miyazawa H, et al.
  • Journal Title: Development. Volume: 137 Pages: 4317-25
  • Peer Reviewed
• [Journal Article] How many peas in a pod? Legume genes responsible for mutualistic symbioses underground. (2010)
  • Author(s): Kouchi H, et al.
  • Journal Title: Plant Cell Physiol Volume: 51 Pages: 1381-97
  • Peer Reviewed
• [Journal Article] Nod factor/nitrate-induced CLE genes that drive HAR1-mediated systemic regulation of nodulation (2009)
  • Author(s): Okamoto, S., Ohnishi, E., Sato, S., Takahashi, H., Nakazono, M., Tabata, S. and Kawaguchi, M
  • Journal Title: Plant Cell Physiol Volume: 50 Pages: 67-77
  • NAID: 10027336479
  • Peer Reviewed
• [Journal Article] TOO MUCH LOVE, a root regulator associated with the long-distance control of nodulation in Lotus japonicus. (2009)
  • Author(s): Magori, S., Oka-Kira, E., Shibata, S.,(他7名, 10番目)
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 22 Pages: 259-268
  • Peer Reviewed
• [Journal Article] Nod factor/nitrate-induced CLE genes that drive HAR1-mediated systemic regulation of nodulation. (2009)
  • Author(s): Okamoto, S., Ohnishi, E., Sato, S., Takahashi, H., Nakazono, M., Tabata, S., Kawaguchi, M.
  • Journal Title: Plant Cell Physiol. 50 Pages: 67-77
  • NAID: 10027336479
  • Peer Reviewed
• [Journal Article] TOO MUCH LOVE, a root regulator associated with the long-distance control of nodulation in Lotus japonicus. (2009)
  • Author(s): Magori, S., Oka-Kira, E., Shibata, S., 他7名
  • Journal Title: Molecular Plant-Microbe Interactions 22 Pages: 259-268
  • Peer Reviewed
• [Journal Article] Conservation of Lotus and Arabidopsis basic helix-loop-helix proteins reveals new players in root hair development. (2009)
  • Author(s): Karas B, et al.
  • Journal Title: Plant Physiology Volume: 151 Pages: 1175-1185
  • Peer Reviewed
• [Journal Article] TOO MUCH LOVE, a root regulator associated with the long-distance control of nodulation in Lotus japonicas. (2009)
  • Author(s): Magori. S, et al.
  • Journal Title: Mol. Plant-Microbe In 22 Pages: 259-268
  • Peer Reviewed
• [Journal Article] Nod factor/nitate-induced CLE genes that drive HAR1-mediated systemic regulation of nodulation. (2009)
  • Author(s): Okamoto S, et al.
  • Journal Title: Plant Cell Phvsiol. 50 Pages: 67-77
  • Peer Reviewed
• [Journal Article] CYCLOPS, a mediator of symbiotic intercellular accommodation. (2008)
  • Author(s): Yano, K., Yoshida, S., Muller, J.,(他16名, 16番目)
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 105 Pages: 20540-5
  • Peer Reviewed
• [Journal Article] CYCLOPS, a mediator of symbiotic intercellular accommodation. (2008)
  • Author(s): Yano, K., Yoshida, S., Muller, J., 他16名
  • Journal Title: Proc.Natl.Acad.Sci.USA 105 Pages: 20540-20545
  • Peer Reviewed
• [Journal Article] CYCLOPS, a mediator of symbiotic intracellular accommodation. (2008)
  • Author(s): Yano K, et al.
  • Journal Title: Proc Natl Acad Sci USA 105 Pages: 20540-20545
  • Peer Reviewed
• [Presentation] Re-evaluation of Arbuscular Mycorrhizal Phenotypes in Root Nodule Symbiosis Mutants Reveals a Novel Common Symbiosis Factor in Lotus japonicus. (2012)
  • Author(s): 武田直也、Martin Parniske、川口正代司
  • Organizer: 日本植物生理学会第53回年会
  • Place of Presentation: 日京都産業大学(京都府)
• [Presentation] ミヤコグサ新規根粒非着生変異体daphneの解析 (2012)
  • Author(s): 養老瑛美子、寿崎拓哉、川口正代司
  • Organizer: 日本植物生理学会第53回年会
  • Place of Presentation: 日京都産業大学(京都府)
• [Presentation] ミヤコグサ新規根粒非着生変異体daphneの解析 (2012)
  • Author(s): 養老瑛美子、寿崎拓哉、川口正代司
  • Organizer: 日本植物生理学会第53回年会
  • Place of Presentation: 京都産業大学(京都府)
• [Presentation] Re-evaluation of Arbuscular Mycorrhizal Phenotypes in Root Nodule Symbiosis Mutants Reveals a Novel Common Symbiosis Factor in Lotus Japonicus. (2012)
  • Author(s): 武田直也、Martin Parniske、川口正代司
  • Organizer: 日本植物生理学会第53回年会
  • Place of Presentation: 京都産業大学(京都府)
• [Presentation] 新奇根粒非着生変異体daphneの表現型解析 (2011)
  • Author(s): 養老瑛美子、寿崎拓哉、川口正代司
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 日東京大学教養学部(東京都)
• [Presentation] 新奇根粒非着生変異体daphneの表現型解析 (2011)
  • Author(s): 養老瑛美子、寿崎拓哉、川口正代司
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京大学教養学部(東京都)
• [Presentation] HAR1 and KLAVIER mediated CLE peptide signaling in long-distance control of nodulation (2010)
  • Author(s): 川口正代司
  • Organizer: 1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: 青島パームビーチホテル(宮崎県)
  • Year and Date: 2010-10-20
• [Presentation] ミヤコグサ菌根共生特異的変異株のマッピングおよび表現型解析 (2010)
  • Author(s): 川口正代司
  • Organizer: 1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: 青島パームビーチホテル(宮崎県)
  • Year and Date: 2010-09-20
• [Presentation] TOO MUCH LOVE :根粒形成の遠距離シグナリングに関わる根の制御因子 (2009)
  • Author(s): 馬郡慎平、吉良(岡)恵利佳、柴田哲、梅原洋佐、河内宏、佐藤修正、田畑哲之、川口正代司
  • Organizer: 日本植物学会第73回大会
  • Place of Presentation: 山形大学(山形県)
• [Presentation] 根粒形成の開始に必要なミヤコグサ推定転写因子NSP1, NSP2の発現変動とフィードバック制御との関連 (2009)
  • Author(s): 村上泰弘、富澤紗織、福井理恵、東久仁子、葉山真歩子、高橋宏和、中園幹生、川口正代司
  • Organizer: 日本植物学会第73回大会
  • Place of Presentation: 山形大学(山形県)
• [Presentation] Nod factor/nitrate-induced CLE genes that drive HAR1-mediated systemic regulation of nodulation (2009)
  • Author(s): Masayoshi Kawaguchi
  • Organizer: International Congress on Nitrogen fixation June
  • Place of Presentation: Montana, USA
• [Presentation] ミヤコグサ菌根共生特異的変異株のマッピングおよび表現型解析 (2009)
  • Author(s): 小島知子
  • Organizer: 植物微生物研究会第19回研究交流会
  • Place of Presentation: あがたの森文化会館(長野県)
• [Presentation] ミヤコグサGRASタンパクLjNSP2の過剰発現による根粒形成パターンの異常 (2008)
  • Author(s): 村上泰弘、富澤紗織、福井理恵、川口正代司
  • Organizer: 日本植物学会第72回大会
  • Place of Presentation: 高知大学(高知県)
• [Presentation] Systemic regulation of nodulation via long-distance signaling (2008)
  • Author(s): Masayoshi Kawaguchi
  • Organizer: 33^<rd> FEBS Congress, 11^<th> IUBMB Conference
  • Place of Presentation: Athens, Greece
• [Book] Physically-induced mutation : ion beam mutagenesis. In The Handbook of Plant Mutation Screening (2010)
  • Author(s): Magori S, Tanaka A and Kawaguchi M
• [Book] Physicallyinduced mutation : ion beam mutagenesis. In The Handbook of Plant Mutation Screening (2010)
  • Author(s): Magori S, Tanaka A, Kawaguchi M.
  • Publisher: Wiley-Blackwell-VCH.
• [Book] Physically-induced mutation : ion beam mutagenesis In The Handbook of Plant Mutation Screening (2010)
  • Author(s): Magori S, et al.
  • Total Pages: 14
  • Publisher: Wiley-Blackwell-VCH.
• [Book] Physically-induced mutation : ion beam mutagenesis. In The Handbook of Plant Mutation Screening (2010)
  • Author(s): Magori S, et al.
  • Publisher: Wiley-Blackwell-VCH.
• [Book] ミヤコグサで根粒菌や菌根菌との共生とその進化を知る研究を支えるモデル生物 (2009)
  • Author(s): 川口正代司
  • Publisher: 化学同人
• [Book] 植物生理学微生物との共生 (2009)
  • Author(s): 川口正代司
  • Publisher: 化学同人
• [Book] ミヤコグサで根粒菌や菌根菌との共生とその進化を知る (2009)
  • Author(s): 川口正代司
  • Publisher: 化学同人
• [Book] アーバスキュラー菌根共生系から根粒共生系への進化 (2008)
  • Author(s): 斎藤勝暗・川口正代司
  • Publisher: 総合出版社文一総合出版社
• [Book] アーバスキュラー菌根共生系から根粒共生系への進化 (2008)
  • Author(s): 斎藤勝暗・川口正代司
  • Publisher: 総合出版社文-総合出版社
• [Book] アーバスキュラー菌根共生系から根粒共生系への進化共進化の生態学種生物学会文 (2008)
  • Author(s): 斎藤勝暗・川口正代司
  • Publisher: 総合出版社
• [Remarks]
  • URL: http://www.nibb.ac.jp/miyakohp/index.html