Regulation of plant redox metabolic function based on the atomic structure of proteins

• Project/Area Number: 20370022
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Osaka University
• Principal Investigator: HASE Toshiharu 大阪大学, 蛋白質研究所, 教授 (00127276)
• Co-Investigator(Renkei-kenkyūsha): KURISU Genji 大阪大学, 蛋白質研究所, 教授 (90294131)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
• Keywords: 蛋白質 / 植物 / 光合成 / 代謝

Research Abstract

Formation of electron transfer complexes of ferredoxin and ferredoxin-dependent redox enzymes involved in carbon, nitrogen and sulfur assimilation has been studied through biochemical and structural biological analyses. Knock-out and knock. down plants of the corresponding enzymes and ferredoxin were also produced to study their functions at in vivo level. Combined results give a new insight into the electron partitioning via the molecular machineries of ferredoxin and related enzymes in photosynthesis.

Research Products

• [Journal Article] A new structural insight into differential interaction of cyanobacterial and plant ferredoxins with nitrite reductase as revealed by NMR and X-ray crystallographic studies (2012)
  • Author(s): Sakakibara Y., Kimura H., Iwamura A., Saitoh T., Ikegami T., Kurisu G., and Hase T.
  • Journal Title: J Biochem Volume: 151 Pages: 483-492
  • NAID: 10031166088
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray studies of an electron-transfer complex of ferredoxin and ferredoxin-dependent glutamate synthase from the cyanobacterium Leptolyngbya boryana (2012)
  • Author(s): Shinmura K., et al
  • Journal Title: Acta crystallographica section F Volume: 68 Issue: 3 Pages: 324-327
  • DOI: 10.1107/s1744309112003387
  • Peer Reviewed
• [Journal Article] A new structural insight into differential interaction of cyanobacterial and plant ferredoxins with nitrite reductase as revealed by NMR and x-ray crystallographic studies (2012)
  • Author(s): Sakakibara Y.
  • Journal Title: J.Biochem. Volume: 151 Issue: 5 Pages: 483-492
  • DOI: 10.1093/jb/mvs028
  • Peer Reviewed
• [Journal Article] Binding energetics of ferredoxin-NADP+ reductase with ferredoxin and its relation to function (2011)
  • Author(s): Lee Y.-H., Ikegami T., Standley D. M., Sakurai K., Hase T., and Goto Y.
  • Journal Title: ChemBioChem Volume: 12 Pages: 2062-2070
  • Peer Reviewed
• [Journal Article] FdC1, a novel ferredoxin protein capable of alternative electron partitioning, increases in conditions of accepter limitation at PSI (2011)
  • Author(s): Voss I., Goss T., Murozuka E., Altman B., McLean K. J., Rigby S. E. J., Munro A. W., Scheibe R., Hase T., and Hanke G. T.
  • Journal Title: J. Biol. Chem Volume: 286 Issue: 1 Pages: 50-59
  • DOI: 10.1074/jbc.m110.161562
  • Peer Reviewed
• [Journal Article] A screen for potential ferredoxin electron transfer partners uncovers new, redox dependent interactions (2011)
  • Author(s): Hanke G. T., Satomi T., Shinmura K., Takao T., and Hase T.
  • Journal Title: Biochim. Biophys. Acta Volume: 1814 Pages: 366-374
  • Peer Reviewed
• [Journal Article] Binding energetics of ferredoxin-NADP^+ reductase with ferredoxin and its relation to junction (2011)
  • Author(s): Lee Y.-H
  • Journal Title: Chem Bio Chem Volume: 12 Pages: 2062-2070
  • Peer Reviewed
• [Journal Article] Identification of N-terminal regions of wheat leaf ferredoxin NADP^+ oxidoreductase important for interactions with ferredoxin. (2011)
  • Author(s): Bowsher C.G., Eyres L.M., Gummadova J.O., Hothi P., McLean K.J., Munro A.W., Scrutton N.S., Hanke G.T., Sakakibara Y., Hase T.
  • Journal Title: Biochemistry 50 Pages: 1778-1787
  • Peer Reviewed
• [Journal Article] FdC1, a novel ferredoxin protein capable of alternative electron partitioning, increases in conditions of accepter limitation at PSI. (2011)
  • Author(s): Voss I., Goss T., Murozuka E., Altman B., McLean K.J., Rigby S.E.J., Munro A.W., Scheibe R., Hase T., Hanke G.T.
  • Journal Title: J.Biol.Chem. 286 Pages: 50-59
  • Peer Reviewed
• [Journal Article] Identification of N-terminal regions of wheat leaf ferredoxin NADP^+ oxidoreductase important for interactions with ferredoxin. (2011)
  • Author(s): Bowsher C.G.
  • Journal Title: Biochemistry Volume: 50 Pages: 1778-1787
  • Peer Reviewed
• [Journal Article] FdCl, a novel ferredoxin protein capable of alternative electron partitioning, increases in conditions of accepter limitation at PSI. (2011)
  • Author(s): Voss I.
  • Journal Title: J.Biol.Chem. Volume: 286 Pages: 50-59
  • Peer Reviewed
• [Journal Article] A screen for potential ferredoxin electron transfer partners uncovers new, redox dependent interactions. (2011)
  • Author(s): Hanke G.T.
  • Journal Title: Biochim.Biophys.Acta. Volume: 1814 Pages: 366-374
  • Peer Reviewed
• [Journal Article] Electron transfer of site-specifically cross-linked complexes between ferredoxin and ferredoxin-NADP+ reductase (2010)
  • Author(s): Kimata-Ariga Y, . Sakakibara Y., Ikegami T., and Hase T.
  • Journal Title: Biochemstry Volume: 49 Issue: 46 Pages: 10013-10023
  • DOI: 10.1021/bi100855a
  • Peer Reviewed
• [Journal Article] Electron transfer of site-specifically cross-linked complexes between ferredoxin and ferredoxin-NADP^+ reductase. (2010)
  • Author(s): Kimata-Ariga Y,. Sakakibara Y., Ikegami T., Hase T.
  • Journal Title: Biochemistry 49 Pages: 10013-10023
  • Peer Reviewed
• [Journal Article] Electron transfer of site-specifically cross-linked complexes between ferredoxin and ferredoxin-NADP+ reductase. (2010)
  • Author(s): Kimata-Ariga Y.
  • Journal Title: Biochemistry Volume: 49 Pages: 10013-10023
  • Peer Reviewed
• [Journal Article] Pattern of expression and substrate specificity of chloroplast ferredoxins from Chlamydomonas reinhardtii (2009)
  • Author(s): Terauchi AM., Lu SF., Zaffagnini M., Tappa S., Hirasawa M., Tripathy JN., Knaff DB., Farmer P. J, Lemaire SD., Hase T., and Merchant SS.
  • Journal Title: J. Biol. Chem Volume: 284 Issue: 38 Pages: 25867-25878
  • DOI: 10.1074/jbc.m109.023622
  • Peer Reviewed
• [Journal Article] Pattern of expression and substrate specificity of chloroplast ferredoxins from Chlamydomonas reinhardtii. (2009)
  • Author(s): Terauchi AM., Lu SF., Zaffagnini M., Tappa S., Hirasawa M., Tripathy JN., Knaff DB., Farmer P.J, Lemaire SD., Hase T., Merchant SS.
  • Journal Title: J.Biol.Chem. 284 Pages: 25867-2587
  • Peer Reviewed
• [Journal Article] Importance of conserved Lys83 residue of Zea mays cytochrome b561 for ascorbate-specific transmembrane electron transfer as revealed by site-directed mutageneis studies. (2009)
  • Author(s): Nakanishi N.
  • Journal Title: Biochemistry 48 Pages: 10665-10678
  • Peer Reviewed
• [Journal Article] Pattern of expression and substrate specificity of chloroplast ferredoxins from Chlamydomonas reinhardtii. (2009)
  • Author(s): Terauchi A.M.
  • Journal Title: J.Biol.Chem. 284 Pages: 25867-25878
  • Peer Reviewed
• [Journal Article] A novel variant of ferredoxin-dependent sulfite reductase having preferred substrata specificity for nitrite in Cvanidioschvzon merolae. (2009)
  • Author(s): Sekine K.
  • Journal Title: Biochem J. 423 Pages: 91-98
  • Peer Reviewed
• [Journal Article] Variable photosynthetic roles of two leaf type ferredoxins in Arabidopsis, as revealed by RNA interference (2008)
  • Author(s): Hanke GT. and Hase T.
  • Journal Title: Photochem. Photobiol Volume: 84 Issue: 6 Pages: 1302-1309
  • DOI: 10.1111/j.1751-1097.2008.00411.x
  • Peer Reviewed
• [Journal Article] Altered photosynthetic electron channeling into cyclic electron flow and nitrite assimilation in a mutant of ferredoxin : NADP(H) reductase (2008)
  • Author(s): Hanke G. T., Endo T., Satoh F., and Hase T.
  • Journal Title: Plant Cell Environ Volume: 31 Issue: 7 Pages: 1017-1028
  • DOI: 10.1111/j.1365-3040.2008.01814.x
  • Peer Reviewed
• [Journal Article] Variable photosynthetic roles of two leaf type ferredoxins in Arabidopsis, as revealed by RNA interference. (2008)
  • Author(s): Hanke GT., Hase T.
  • Journal Title: Photochem. Photobiol. 84 Pages: 1302-130
  • Peer Reviewed
• [Journal Article] Altered photosynthetic electron channeling into cyclic electron flow and nitrite assimilation in a mutant of ferredoxin : NADP (H) reductase. (2008)
  • Author(s): Hanke G.T., Endo T., Satoh F., Hase T.
  • Journal Title: Plant Cell Environ. 31 Pages: 1017-1028
  • Peer Reviewed
• [Journal Article] Variable photosynthetic roles of two leaf type ferredoxin in Arabidopsis, asrevealed by RNA interference (2008)
  • Author(s): Hanke G. T.
  • Journal Title: Photochem. Photobiol. 84 Pages: 1302-1309
  • Peer Reviewed
• [Journal Article] Implication of the glutamine synthetase-glutamate synthase pathway inconditioning the amino acid metabolism in bundle sheath and mesophyll cellsof maize leaves (2008)
  • Author(s): Valadier M-H.
  • Journal Title: FEBS J. 275 Pages: 3193-3206
  • Peer Reviewed
• [Journal Article] Altered photosynthetic electron channeling into cyclic electron flow and nitriteassimilation in a mutant of ferredoxin : NADP(H) reductase (2008)
  • Author(s): Hanke G. T.
  • Journal Title: Plant Cell Environ. 31 Pages: 1017-1028
  • Peer Reviewed
• [Presentation] ラン藻Leptolyngbya boryanaのFd/Fd-GOGAT電子伝達複合体のX線結晶構造解析 (2012)
  • Author(s): 新村佳奈子
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-03-17
• [Presentation] フェレドキシンと亜硫酸還元酵素の電子伝達複合体の構造と分子間相互作用の解析 (2011)
  • Author(s): 金宙妍
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-22
• [Presentation] 葉緑体チラコイド膜を用いたFd-FNRクロスリンク複合体の電子分配機構の解析 (2011)
  • Author(s): 有賀洋子
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-22
• [Presentation] シロイヌナズナFTRのヘテロダイマーの性状とチオレドキシンとの相互作用 (2010)
  • Author(s): 波多野愛未
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2010-12-10
• [Presentation] Electron Transfer of Site-specifically Cross-linked Complexes between Ferredoxin and Ferredoxin-NADP^+ Reductase (2010)
  • Author(s): 有賀洋子
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2010-12-07
• [Presentation] 真正粘菌Physarum polycephalumの分泌型β-glucosidaseの解析 (2010)
  • Author(s): 守安美幸
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2010-12-07
• [Presentation] A novel variant of ferredoxin-dependent sulfite reductase having preferred nitrite-reducing activity (2010)
  • Author(s): 関根康介
  • Organizer: 1^<st> International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: Inuyama Int.Sightseeing Center "FREUDE"
  • Year and Date: 2010-07-28
• [Presentation] Differential roles for Fd-and NADH-GOGAT in concerted photosynthetic nitrogen and carbon assimilations in the cyanobacterium Leptolyngbya boryana (2010)
  • Author(s): 新村佳奈子
  • Organizer: 1^<st> International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: Inuyama Int.Sightseeing Center "FREUDE"
  • Year and Date: 2010-07-27
• [Presentation] Study on protein-protein interaction of ferredoxin and ferredoxin-dependent glutamate synthase (Fd-GOGAT) (2010)
  • Author(s): 榊原由紀子
  • Organizer: 1^<st> International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: Inuyama Int.Sightseeing Center "FREUDE"
  • Year and Date: 2010-07-27
• [Presentation] Mutational study of maize glutamine synthetase(GSla) : Identification of two distinct sites necessary for high affinity for glutamate (2010)
  • Author(s): 尾崎健
  • Organizer: 1^<st> International Symposium on the Nitrogen Nutrition of Plants
  • Place of Presentation: Inuyama Int.Sightseeing Center "FREUDE"
  • Year and Date: 2010-07-27
• [Presentation] 植物葉緑体のレドックスネットワークの分子基盤と光合成機能の制御 (2010)
  • Author(s): 長谷俊治
  • Organizer: 岡崎統合バイオサイエンスセンター10周年記念シンポジウム
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2010-02-11
• [Presentation] Structural basis for molecular recognition of Fd and Fd-dependent proteins。 (2010)
  • Author(s): Hase T
  • Organizer: Arnon Centennial Symposium
  • Place of Presentation: Asilomar Conference Ground, California, USA
• [Presentation] Structural basis for molecular recognition of Fd and Fd-dependent proteins. (2010)
  • Author(s): Hase T
  • Organizer: Arnon Centennial Symposium
  • Place of Presentation: Asilomar, California
• [Presentation] Structural basis for molecular recognition of Fd and Fd-dependent proteins (2010)
  • Author(s): Toshiharu Hase
  • Organizer: Arnon Centennial Symposium
  • Place of Presentation: Asilomar Conference Ground, California, USA
• [Presentation] Cyanidioschyzon merolaeにおける亜硝酸を優位に還元する新規亜硫酸還元酵素 (2010)
  • Author(s): 関根康介
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学
• [Presentation] トウモロコシFdsとFNRsを用いた分子特性の解析 (2009)
  • Author(s): 篠原文男
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2009-10-24
• [Presentation] シロイヌナズナに見いだされた新規フェレドキシンホモログの解析(第2報) (2009)
  • Author(s): 金田大輝
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際展示場
  • Year and Date: 2009-10-24
• [Presentation] 植物グルタミン合成酵素の基質認識機構の研究 : グルタミン酸への親和性を決定する活性中心遠位領域の同定 (2009)
  • Author(s): 尾崎健
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-10-22
• [Presentation] Cyanidioschyzon merolaeにおける亜硝酸を選択的に還元する新規亜硫酸還元酵素 (2009)
  • Author(s): 関根康介
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-10-22
• [Presentation] フェレドキシンからの電子分配のダイナミクス (2009)
  • Author(s): 長谷俊治
  • Organizer: 学術創成シンポジウム光合成研究の道標-現在地と地平の先-
  • Place of Presentation: 京都大学
  • Year and Date: 2009-07-10
• [Presentation] フェレドキシンからの電子分配のダイナミクス (2009)
  • Author(s): 長谷俊治
  • Organizer: 学術創成シンポジウム「光合成研究の道標-現在地と地平の先-」
  • Place of Presentation: 京都大学
  • Year and Date: 2009-07-10
• [Presentation] Structure analysis of synthetic complexes obtained through inter-moleculardisulfide bond between ferredoxin and ferredoxin-NADP^+reductase (2008)
  • Author(s): Ariga Y.
  • Organizer: Biochemistry and Molecular Biology 2008
  • Place of Presentation: ワールド記念ホール(神戸)
  • Year and Date: 2008-12-11
• [Presentation] 植物グルタミン酸合成酵素とフェレドキシンとの分子間相互作用と電子伝達の解析 (2008)
  • Author(s): 吉田 綾子
  • Organizer: Biochemistry and Molecular Biology 2008
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2008-12-11
• [Presentation] シロイヌナズナに見出された新規フェレドキシンホモログの解析 (2008)
  • Author(s): 金田 大輝
  • Organizer: Biochemistry and Molecular Biology 2008
  • Place of Presentation: ワールド記念ホール(神戸)
  • Year and Date: 2008-12-10
• [Presentation] Mutational study of the contact region of two pentamer rings of maize GSla (2008)
  • Author(s): Yamada M.
  • Organizer: Biochemistry and Molecular Biology 2008
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2008-12-09
• [Presentation] Structural basis for redox cascades in chloroplasts and nonphotosynthetic plastids (2008)
  • Author(s): Hase T
  • Organizer: Chloroplast Biology
  • Place of Presentation: 中央研究院、台北
• [Presentation] Structural basis for redox cascades in chloroplasts and nonphotosynthetic plastids. (2008)
  • Author(s): Hase T
  • Organizer: Chloroplast Biology
  • Place of Presentation: IPMB Acaemia Sinica, Taipei
• [Presentation] Structural basis for redox cascades in chloroplasts and nonphotosyntheticplastids (2008)
  • Author(s): Hase T.
  • Organizer: Chloroplast Biology
  • Place of Presentation: 中央研究院、台北