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Regulation of microtubule cytoskeleton by phosphorylation

Research Project

Project/Area Number 20370023
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionNara Institute of Science and Technology

Principal Investigator

HASHIMOTO Takashi  Nara Institute of Science and Technology, バイオサイエンス研究科, 教授 (80180826)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2009: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2008: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywords微小管 / リン酸化 / フォスファターゼ / キナーゼ / アラビドプシス / アラピドプシス / シグナル伝達 / 植物
Research Abstract

Phosphorylation of plant microtubules was investigated with respects to tubulin phosphorylation and protein phosphatases. I found that the Arabidopsis Propyzamide-Hypersensitive 1 protein of the mitogen-activated protein kinase phosphatase family also possesses a kinase domain of atypical protein kinases. The kinase activity is suppressed by the intra-molecular phosphatase activity, but when this suppression is removed, the activated kinase rapidly phosphorylates tubulin and depolymerizes cortical microtubules.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (37 results)

All 2011 2010 2009 2008 Other

All Journal Article (14 results) (of which Peer Reviewed: 12 results) Presentation (21 results) Remarks (2 results)

  • [Journal Article] Microtubule and cell shape determination.2011

    • Author(s)
      T.Hashimoto
    • Journal Title

      In: The Plant Cytoskeleton, B.Liu, ed (Springer, New York) Chapter 11

      Pages: 245-257

    • Related Report
      2010 Final Research Report
  • [Journal Article] Microtubule and katanin dependent dynamics of microtubule nucleation complexes in the Arabidopsis cortical array.2010

    • Author(s)
      M.Nakamura, D.Ehrhardt, T.Hashimoto
    • Journal Title

      Nature Cell Biol. 12

      Pages: 1064-1070

    • NAID

      130006993218

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mitogen-activated protein kinase phosphatase PHS1 is retained in the cytoplasm by nuclear extrusion signal-dependent and independent mechanisms.2010

    • Author(s)
      J.Pytela, T.Kato, T.Hashimoto
    • Journal Title

      Planta 231

      Pages: 1311-1322

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gravity-induced modifications to development in hypocotyls of Arabidopsis tubulin mutants.2010

    • Author(s)
      S.Matsumoto, S.Kumasaki, K.Soga, K.Wakabayashi, T.Hashimoto, T.Hoson
    • Journal Title

      Plant Physiol. 152

      Pages: 918-926

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nuclear-localized subtype of end-binding 1 protein regulates spindle organization in Arabidopsis.2010

    • Author(s)
      S.Komaki, T.Abe, S.Coutuer, D.Inze, E.Russinova,T.Hashimoto
    • Journal Title

      J.Cell Sci. 123

      Pages: 451-459

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nuclear-localized subtype of end-binding 1 protein regulates spindle organization in Arabidopsis.2010

    • Author(s)
      S.Komaki
    • Journal Title

      J.Cell Sci.

      Volume: 123 Pages: 451-459

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microtubule and katanin dependent dynamics of microtubule nucleation complexes in the Arabidopsis cortical array.2010

    • Author(s)
      M.Nakamura
    • Journal Title

      Nature Cell Biol.

      Volume: 12 Pages: 1064-1070

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mitogen-activated protein kinase phosphatase PHS1 is retained in the cytoplasm by nuclear extrusion signal-dependent and independent mechanisms.2010

    • Author(s)
      J.Pytela
    • Journal Title

      Planta

      Volume: 231 Pages: 1311-1322

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mitogen-activated protein kinase phosphatase PHSI is retained in the cytoplasm by nuclear extrusion signal-dependent and independent mechanisms.2010

    • Author(s)
      J.Pytela
    • Journal Title

      Planta 231

      Pages: 1311-1322

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GTP is required for the microtubule catastrophe-inducing activity of MAP200, a tobacco homolog of XMAP215.2009

    • Author(s)
      T.Hamada, T.J.Itoh, T.Hashimoto, T.Shimmen, S.Sonobe
    • Journal Title

      Plant Physiol. 151

      Pages: 1823-1830

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A mutation in the Arabidopsis.γ-tubulin-containing complex subunit causes helical growth and abnormal microtubule branching.2009

    • Author(s)
      M.Nakamura, T.Hashimoto
    • Journal Title

      J.Cell Sci. 122

      Pages: 2208-2217

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis SPIRAL2 promotes uninterrupted microtubule growth by suppressing the pause state of microtubule dynamics.2008

    • Author(s)
      M.Yao, Y.Wakamatsu, T.J.Itoh, T.Shoji, T.Hashimoto
    • Journal Title

      J.Cell Sci. 121

      Pages: 2372-2381

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The cytoskeleton and signal transduction : Role and regulation of plant actin and microtubule binding proteins.2008

    • Author(s)
      P.Hussey, T.Hashimoto
    • Journal Title

      In : Intracellular Signalling in Plants, (ed.Z.Yang), Wiley-Blackwell

      Pages: 244-272

    • Related Report
      2010 Final Research Report
  • [Journal Article] Arabidopsis SPIRAL2 promotes uninterrupted microtubule growthby suppressing the pause state of microtubule dynamics.2008

    • Author(s)
      M. Yao
    • Journal Title

      J. Cell Sci. 121

      Pages: 2372-2381

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] PHS1の新奇キナーゼ様ドメインは微小管脱重合を促進する2011

    • Author(s)
      藤田智史, Jaromir Pytela、加藤壮英、乾良充、神戸雅人、橋本隆
    • Organizer
      第52回日本植物生理学会年会
    • Related Report
      2010 Final Research Report
  • [Presentation] Organization of cortical microotubule arrays in Arabidopsis2010

    • Author(s)
      橋本隆
    • Organizer
      Gordon research conference
    • Place of Presentation
      Andover, USA
    • Year and Date
      2010-08-11
    • Related Report
      2010 Final Research Report
  • [Presentation] Regulation of cortical microtubule arrays in plants2010

    • Author(s)
      T.Hashimoto
    • Organizer
      Gordon Conference
    • Place of Presentation
      Proctor Academy, NH, USA
    • Year and Date
      2010-08-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] mRNA代謝における細胞骨格ネットワークの役割2010

    • Author(s)
      濱田隆宏、深谷雄志、渡辺雄一郎、橋本隆
    • Organizer
      第51回日本植物生理学会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-21
    • Related Report
      2010 Final Research Report
  • [Presentation] アラビドプシスにおいて核局在型EB1は紡錘体の編成に必要である2010

    • Author(s)
      小牧伸一郎, 阿部竜也, Silvie Coutuer, Dirk Inze, EugeniaRussinova, 橋本隆
    • Organizer
      第51回日本植物生理学会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-21
    • Related Report
      2010 Final Research Report
  • [Presentation] 方向依存的な細胞伸長に関わる新規因子ITOSUGIの機能解析2010

    • Author(s)
      八木慎宜、檜垣マリコ、槻木竜二、岡田清孝、橋本隆
    • Organizer
      第51回日本植物生理学会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-21
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物の表層微小管を制御するPHS1の機能解析2010

    • Author(s)
      藤田智史、Pytela Jaromir, 加藤壮英、橋本隆
    • Organizer
      第51回日本植物生理学会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-21
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物細胞の表層微小管形成における微小管重合核の動的挙動の解析2010

    • Author(s)
      中村匡良、Daivid W.Ehrhardt、橋本隆
    • Organizer
      第51回日本植物生理学会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-21
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物の表層微小管を制御するPHSIの機能解析2010

    • Author(s)
      藤田智史
    • Organizer
      日本植物生理学会
    • Place of Presentation
      熊本
    • Year and Date
      2010-03-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] Ehrhardt, Takashi Hashimoto, Analysis of dynamical behavior of microtubule-nucleating complexes in the cortical microtubule nucleation of Arabidopsis cells2010

    • Author(s)
      Masayoshi Nakamura, David W.
    • Organizer
      Gordon research conference
    • Place of Presentation
      Andover, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] Helical Asymmetry and Regulation of Cortical Microtubule Arrays in Plants2009

    • Author(s)
      橋本隆
    • Organizer
      16th International Soceity of Developmental Biologists Congress
    • Place of Presentation
      Edinburgh, UK
    • Year and Date
      2009-09-08
    • Related Report
      2010 Final Research Report
  • [Presentation] Live cell imaging of cortical microtubule nucleation events in Arabidopsis cells2009

    • Author(s)
      Masayoshi Nakamura, David W.Ehrhardt, Takashi Hashimoto
    • Organizer
      Plant Biology
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2009-07-20
    • Related Report
      2010 Final Research Report
  • [Presentation] AtEB1cは正常な染色体分離と分裂期微小管の配置に必要である2009

    • Author(s)
      小牧伸一郎, 橋本隆
    • Organizer
      第50回植物生理学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-23
    • Related Report
      2010 Final Research Report
  • [Presentation] シロイヌナズナ生細胞における微小管形成のライブイメージング2009

    • Author(s)
      中村匡良、Ehrhardt DW, 橋本隆
    • Organizer
      第50回植物生理学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-23
    • Related Report
      2010 Final Research Report
  • [Presentation] 新規微小管関連因子PROPYZAMIDE HYPERSENSITIVE2 (PHS2)の機能解析2009

    • Author(s)
      加藤壮英, 直井国子, 橋本隆
    • Organizer
      第50回植物生理学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-23
    • Related Report
      2010 Final Research Report
  • [Presentation] シロイヌナズナ微小管付随タンパク質群のショットガン解析2009

    • Author(s)
      濱田隆宏、深尾陽一朗、藤原正幸、小牧伸一郎、中村匡良、園部誠司、橋本隆
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-23
    • Related Report
      2010 Final Research Report
  • [Presentation] Organization of cortical microtubule arrays in non-centrosomal plant cells2008

    • Author(s)
      橋本隆
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2010 Final Research Report
  • [Presentation] Organization of cortical microtubule arrays in non-centrosomal plant cells2008

    • Author(s)
      橋本 隆
    • Organizer
      日本分子生物学会・日本生化学会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] Functional analysis of Arabidopsis EB1 protein family2008

    • Author(s)
      Shinichiro Komaki, Tatsuya Abe, Silvie Coutuer, Joanna Boruc, Dirk Inze, Eugenia Russinova, Takashi Hashimoto
    • Organizer
      19th International Conference on Arabidopsis Research
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      2008-07-24
    • Related Report
      2010 Final Research Report
  • [Presentation] Microtubule-nucleation complexes contribute cortical microtubule array organization in Arabidopsis cells2008

    • Author(s)
      中村匡良, 橋本隆
    • Organizer
      19th International Conference on Arabidopsis Research
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      2008-07-24
    • Related Report
      2010 Final Research Report
  • [Presentation] Analysis of propyzamide hypersensitive2 (phs2) mutant, related with microtubule regulation in Arabidopsis2008

    • Author(s)
      Takehide Kato, Kuniko Naoi, Takashi Hashimoto
    • Organizer
      19th International Conference on Arabidopsis Research
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      2008-07-24
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページアドレス

    • URL

      http://bsw3.naist.jp/courses/courses103.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://bsw3.naist.jp/hashimoto/hashimoto.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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