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Comprehensive analyses on novel functions of plant peroxisomes

Research Project

Project/Area Number 20370024
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionNational Institute for Basic Biology

Principal Investigator

NISHIMURA Mikio  基礎生物学研究所, 高次細胞機構研究部門, 教授 (80093061)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Keywordsペルオキシソーム / トランスクリプトーム解析 / ポリアミン / プロテオーム / ワックス合成 / ペルオキシソーム形成因子 / 植物生殖過程 / Pex1-Pex6複合体 / ペルオキシソーム膜タンパク質38 / ペルオキシソーム形態不全変異株 / ミトコンドリアキャリーファミリー / 器官の融合 / 2次元ポリアクリルアミド電気泳動 / ポリアミンオキシターゼ / 根組織 / スペルミン / スペルミジン
Research Abstract

To clarify the novel functions of peroxisomes in higher plants, transcriptome and proteome analyses were carried out. As a result, it is found that root peroxisomes engage in polyamine catabolism and peroxisomes in epidermal cells of leaves and stems are involved in wax production. The analysis of dsRNAi knock-down mutants of peroxin provides an evidence that peroxisomes are essential for fertilization in plants.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (57 results)

All 2012 2011 2010 2009 2008 Other

All Journal Article (53 results) (of which Peer Reviewed: 53 results) Book (4 results)

  • [Journal Article] Defects of peroxisomal membrane protein 38 causes enlargement of peroxisomes2011

    • Author(s)
      Mano, S., C. Nakamori, Y. Fukao, M. Araki, A. Matsuda, M. Kondo and M. Nishimura
    • Journal Title

      Plant Cell Physiol

      Volume: 52 Pages: 2157-2172

    • NAID

      10030306536

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis ABERRANT PEROXISOME MORPHOLOGY9 is a peroxin that recruts of the PEX1/PEX6 complex to peroxisomes2011

    • Author(s)
      Goto, S., S. Mano, C. Nakamori and M. Nishimura
    • Journal Title

      Plant Cell

      Volume: 23 Pages: 1573-1587

    • Related Report
      2010 Annual Research Report 2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Defects of peroxisomal membrane protein 38 causes enlargement of peroxisomes2011

    • Author(s)
      Mano Shoji
    • Journal Title

      Plant Cell Physiol.

      Volume: 52 Pages: 2157-2172

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel monogalactosyldiacylglycerol synthase found in green sulfur bacteria reveals new roles of galactolipids in photosynthesis2011

    • Author(s)
      Masuda Shinji
    • Journal Title

      Plant Cell

      Volume: 23 Pages: 2644-2658

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Plant Organelles Database 2 (PODB2) : An update containing movie data of plant organelle dynamics2011

    • Author(s)
      Mano Shoji
    • Journal Title

      Plant Cell Physiol

      Volume: 52 Pages: 244-253

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Plant Organelles Database 2 (PODB2) : an updated resource containing movie data of plant organelle dynamics.2011

    • Author(s)
      Mano Shoji
    • Journal Title

      Plant Cell Physiol.

      Volume: 52 Pages: 244-253

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A peroxisomal ABC transporter promotes seed germination by suppressing polygalacturonase inhibiting proteins under the control of ABI52010

    • Author(s)
      Kanai, M., M. Nishimura and M. Hayashi
    • Journal Title

      Plant J

      Volume: 62 Pages: 936-947

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis Qa-SNARE SPY2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development2010

    • Author(s)
      Shirakawa Makoto
    • Journal Title

      Plant J.

      Volume: 64 Pages: 924-935

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gateway binary vectors with bialaphos resistant gene, bar, as the selection marker for plant transformation2010

    • Author(s)
      Nakamura Shinya
    • Journal Title

      Biosci.Biotech.Biochem.

      Volume: 74 Pages: 1315-1319

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A peroxisomal ABC transporter promotes seed germination by suppressing polygalacturonase inhibiting proteins under the control of ABI52010

    • Author(s)
      Kanai Masatake
    • Journal Title

      Plant J.

      Volume: 62 Pages: 936-947

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ectopic expression of an esterase, which is a candidate for the unidentified plant cutinase, causes cuticular defects in Arabidopsis thaliana2010

    • Author(s)
      Takahashi Kentaro
    • Journal Title

      Plant Cell Physiol.

      Volume: 51 Pages: 123-131

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unique defence strategy by the endoplasmic reticulum body in plants2010

    • Author(s)
      Yamada Kenji
    • Journal Title

      Plant Cell Physiol

      Volume: 52 Pages: 2039-2049

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development.2010

    • Author(s)
      Shirakawa Makoto
    • Journal Title

      Plant J.

      Volume: 64 Pages: 924-935

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gateway binary vectors with the bialaphos resistance gene, bar, as a selection marker for plant transformation.2010

    • Author(s)
      Nakamura Shinya
    • Journal Title

      Biosci.Biotechnol.Biochem.

      Volume: 74 Pages: 1315-1319

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A peroxisomal ABC transporter promotes seed germination by inducing pectin degradation under the control of ABI5.2010

    • Author(s)
      Kanai Masatake
    • Journal Title

      Plant J.

      Volume: 62 Pages: 936-947

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ectopic expression of an esterase, which is a candidate for the unidentified plant cutinase, causes cuticular defects in Arabidopsis thaliana.2010

    • Author(s)
      Takahashi Kentaro
    • Journal Title

      Plant Cell Physiol.

      Volume: 51 Pages: 123-131

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Suppression of peroxisome biogenesis factor 10 reduced cuticular wax accumulation by disrupting the ER network in Arabidopsis thaliana2009

    • Author(s)
      Kamigaki, A., M. Kondo, S. Mano, M. Hayashi and M. Nishimura
    • Journal Title

      Plant Cell Physiol

      Volume: 50 Pages: 2034-2046

    • NAID

      10027344776

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular components required for the targeting of PEX7 to peroxisomesin Arabidopsis thaliana2009

    • Author(s)
      Singh, T., M. Hayashi, S. Mano, Y. Arai, S. Goto and M. Nishimura
    • Journal Title

      Plant J

      Volume: 60 Pages: 488-498

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis dynamin-related proteins DRP3A and DRP3B are functionallyredundant in mitochondrial fission, but have distinct roles in peroxisomal fission2009

    • Author(s)
      Fujimoto, M., S. Arimura, S. Mano, M. Kondo, C. Saito, T. Ueda, M. Nakazono, A. Nakano, M. Nishimura and N. Tsutsumi
    • Journal Title

      Plant J

      Volume: 58 Pages: 388-400

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Frontiers of research on organelle differentiation2009

    • Author(s)
      Hayashi, M. and M. Nishimura
    • Journal Title

      Plant Cell Physiol

      Volume: 50 Pages: 1995-1999

    • NAID

      10027344578

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] An isoform of Arabidopsis myosin XI interacts with small GTPases in its C-terminal tail region2009

    • Author(s)
      Hashimoto, K., H. Igarashi, S. Mano, C. Takenaka, T. Shiina, M. Yamaguchi, T. Demura, M. Nishimura, T. Shimmen, E. Yokota
    • Journal Title

      J. Exp. Bot

      Volume: 59 Pages: 3523-3531

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Suppression of peroxisome biogenesis factor 10 reduced cuticular wax accumulation by disrupting the ER network in Arabidopsis thaliana.2009

    • Author(s)
      Kamigaki Akane
    • Journal Title

      Plant Cell Physiol.

      Volume: 50 Pages: 2034-2046

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GNOM-LIKE1/ERMO1 and SEC24a/ERMO2 are required for maintenance of endoplasmic reticulum morphology in Arabidopsis thaliana.2009

    • Author(s)
      Nakano T. Ryohei
    • Journal Title

      Plant Cell

      Volume: 21 Pages: 3672-3685

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Peroxisomes are required for in vivo nitric oxide accumulation in the cytosol following salinity stress of Arabidopsis plants.2009

    • Author(s)
      Francisco J. Corpas
    • Journal Title

      Plant Physiol.

      Volume: 151 Pages: 2083-2094

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel membrane fusion-mediated plant immunity against bacterial pathogens.2009

    • Author(s)
      Hatsugai Noriyuki
    • Journal Title

      Genes Dev.

      Volume: 23 Pages: 2496-2506

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular components required for the targeting of PEX7 to peroxisomes in Arabidopsis thaliana.2009

    • Author(s)
      Tanuja singh
    • Journal Title

      Plant J.

      Volume: 60 Pages: 488-498

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gibberellin modulates anther development by the transcriptional regulation of GAMYB.2009

    • Author(s)
      Aya Koichiro
    • Journal Title

      Plant Cell

      Volume: 21 Pages: 1453-1472

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Vacuolar iron transporter, TgVit, in tulip is responsible for blue coloration in petal cells through iron accumulation.2009

    • Author(s)
      Momonoi Kaori
    • Journal Title

      Plant J.

      Volume: 59 Pages: 437-447

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Constitutive and inducible ER bodies of Arabidopsis thaliana accumulate distinct β-glucosidases.2009

    • Author(s)
      Ogasawara Kimi
    • Journal Title

      Plant Cell Physiol.

      Volume: 50 Pages: 480-488

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis dynamin-related proteins DRP3A and DRP3B are functionally redundant in mitochondrial fission, but have distinct roles in peroxisomal fission.2009

    • Author(s)
      Fujimoto Masaru
    • Journal Title

      Plant J.

      Volume: 58 Pages: 388-400

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Frontiers of research on organelle differentiation.2009

    • Author(s)
      Hayashi Makoto
    • Journal Title

      Plant Cell Physiol.

      Volume: 50 Pages: 1995-1999

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seeing is believing : On the use of image databases for visually exploring plant organelle dynamics.2009

    • Author(s)
      Mano Shoji
    • Journal Title

      Plant Cell Physiol.

      Volume: 50 Pages: 2000-2014

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ER body, a new organelle in Arabidopsis thaliana, requires NAI2 to its formation and accumulates specific β-glucosidase.2009

    • Author(s)
      Yamada Kenji
    • Journal Title

      Plant Signal.Behav.

      Volume: 4 Pages: 849-852

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic identification and characterization of a novel peroxisomal adenine nucleotide transporter supplying ATP for fatty acidβ-oxidation in soybean and Arabidopsis2008

    • Author(s)
      Arai, Y., M. Hayahi and M. Nishimura
    • Journal Title

      Plant Cell

      Volume: 20 Pages: 3227-3240

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic analysis of highly purified peroxisomes from etiolated soybean cotyledons2008

    • Author(s)
      Arai, Y., M. Hayashi and M. Nishimura
    • Journal Title

      Plant Cell Physiol

      Volume: 49 Pages: 526-539

    • NAID

      10027330156

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A putative peroxisomal polyamine oxidase, AtPAO4, is involved in polyamine catabolism in Arabidopsis thaliana2008

    • Author(s)
      Kamada-Nobusada, T., M. Hayashi, M. Fukazawa H. Sakakibara and M. Nishimura
    • Journal Title

      Plant Cell Physiol

      Volume: 49 Pages: 1272-1282

    • NAID

      10027333301

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plant catalase is imported into peroxisomes by Pex5p but is distinct from typical PTS1 import2008

    • Author(s)
      Oshima, Y., A. Kamigaki, C. Nakamori, S. Mano, M. Hayashi, M. Nishimura and M. Esaka
    • Journal Title

      Plant J

      Volume: 49 Pages: 671-677

    • NAID

      10027330794

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic identification and characterization of a novel peroxisomal adenine nucleotide transporter supplying ATP for fatty acid β-oxidation in soybean and Arabidopsis2008

    • Author(s)
      Arai Yuko
    • Journal Title

      Plant Cell 20

      Pages: 3227-3240

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic analysis of highly purified peroxisomes from etiolated soybean cotyledons2008

    • Author(s)
      Arai Yuko
    • Journal Title

      Plant Cell Physiol 49

      Pages: 526-539

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An isoform of Arabidopsis myosin XI interacts with small GTPases in its C-terminal tail region2008

    • Author(s)
      Hashimoto Kohsuke
    • Journal Title

      J.Exp.Bot. 59

      Pages: 3523-3531

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A putative peroxisomal polyamine oxidase, AtPAO4, is involved in polyamine catabolism in Arabidopsis thaliana2008

    • Author(s)
      Kamada-Nobusada Tomoe
    • Journal Title

      Plant Cell Physiol 49

      Pages: 1272-1282

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] NAC family proteins NARS11/NAC2 and NARS2/NAM in the outer integument regulate embryogenesis in Arabidopsis2008

    • Author(s)
      Kunieda Tadashi
    • Journal Title

      Plant Cell 20

      Pages: 2631-2642

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The plant organelles database(PODB) : A collection of visualized Plant organelles and protocols for plant organelle research2008

    • Author(s)
      Mano Shoji
    • Journal Title

      Nucleic Acids Res. 36

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Localization of myo-inositol-1-phosphate synthase to the endosperm in developing seeds of Arabidopsis2008

    • Author(s)
      Mitsuhashi Naoto
    • Journal Title

      J.Exp.Bot. 59

      Pages: 3069-3076

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antagonistic jacalin-related lectins regulate the size of ER body-type β-glucosidase complexes in Arabidopsis thaliana2008

    • Author(s)
      Nagano Atsushi
    • Journal Title

      Plant Cell Physiol 49

      Pages: 969-980

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] AtMapl : a DNA microarray for genomic deletion mapping in Arabidopsis thaliana2008

    • Author(s)
      Nagano Atsushi
    • Journal Title

      Plant J. 56

      Pages: 1058-1065

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plant catalase is imported into peroxisomes by Pex5p but is distinct from typical PTS1 import2008

    • Author(s)
      Oshima Yoshimi
    • Journal Title

      Plant J. 49

      Pages: 671-677

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the OsOPR7 gene encoding 12-oxophytodienoate reductase involved in the biosynthesis of jasmonic acid in rice2008

    • Author(s)
      Tani Tomoyuki
    • Journal Title

      Planta 227

      Pages: 517-526

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] NAI2 is an endoplasmic reticulum body component that enables ER body formation in Arabidopsis thaliana2008

    • Author(s)
      Yamada Kenji
    • Journal Title

      Plant Cell 20

      Pages: 2529-2540

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cytosolic heat shock protein 90 regulates heat shock transcription factor in Arabidopsis thaliana2008

    • Author(s)
      Yamada Kenji
    • Journal Title

      Plant Signal.Behav 3

      Pages: 660-662

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis VPS35, a retromer component, is required for vacuolar protein sorting and involved in plant growth and leaf senescence2008

    • Author(s)
      Yamazaki Misako
    • Journal Title

      Plant Cell Physiol 49

      Pages: 142-156

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A possible involvement of autophagy in amyloplast degradation in columella cells during hydrotropic response of Arabidopsis roots

    • Author(s)
      Nakayama Mayumi
    • Journal Title

      Planta

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis ABERRANT PEROXISOME MORPHOLOGY 9 is a peroxin that recruits the PEX1-PEX6 complex to peroxisomes.

    • Author(s)
      Goto Shino
    • Journal Title

      Plant Cell

      Volume: (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Book] Genetic Engineering-Basics, New Applications and Responsibilities2012

    • Author(s)
      Tanaka Yuji
    • Total Pages
      27
    • Publisher
      InTech
    • Related Report
      2010 Annual Research Report
  • [Book] Genetic Engineering-Basics, New Applications and Responsibilities2011

    • Author(s)
      Tanaka, Y., T. Kimura, K. Hikino, S. Goto and M. Nishimura
    • Publisher
      InTech
    • Related Report
      2010 Final Research Report
  • [Book] Plant Proteomics : Technologies, Strategies and Applications2008

    • Author(s)
      Arai, Y., Y. Fukao, M. Hayashi and M. Nishimura
    • Related Report
      2010 Final Research Report
  • [Book] Peroxisome In Plant Proteomics : Technologies, Strategies and Applications2008

    • Author(s)
      Arai Yuko
    • Total Pages
      764
    • Publisher
      Jhon Wiley & Sons
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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