Microscopic analyses of chromosomal structure by functional knockdown using a photosensitizer

• Project/Area Number: 20370027
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Osaka University
• Principal Investigator: MATSUNAGA Sachihiro Osaka University, 工学研究科, 准教授 (40323448)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2008: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
• Keywords: 染色体 / 細胞分裂 / 蛍光タンパク質 / 細胞周期 / タンパク質 / イメージング

Research Abstract

The photosensitizer is a fluorescence protein that efficiently generates reactive oxygen species (ROS) in the-expressing cells upon intense green light illumination. The functional knockdown method using the photosensitizer was developed in this project.

Research Products

• [Journal Article] Programmed induction of endoreduplication by DNA double-strand breaks in Arabidopsis. (2011)
  • Author(s): Adachi, S., Minamisawa, K., Okushima, Y., Inagaki, S., Yoshiyama, K., Kondou, Y., Kaminuma, E., Kawashima, M., Toyoda, T., Matsui, M., Kurihara, D., Matsunaga, S., Umeda, M.
  • Journal Title: Proc.Natl.Acad.Sci.USA in press
  • NAID: 120005716659
  • Peer Reviewed
• [Journal Article] Identification and characterization of plant Haspin kinase as a histone H3 threonine kinase. (2011)
  • Author(s): Kurihara, D., Matsunaga, S., Omura, T., Higashiyama, T., Fukui K.
  • Journal Title: BMC Plant Biol. 11 Pages: 73-73
  • NAID: 120006995017
  • Peer Reviewed
• [Journal Article] Assembly states of the nucleosome assembly protein 1 (NAP-1) revealed by sedimentation velocity and non-denaturing mass spectrometry. (2011)
  • Author(s): Noda, M., Uchiyama, S., McKay, A.R., Morimoto, A., Misawa, S., Yoshida, A., Shimahara, H., Takinowaki, H., Nakamura, S., Kobayashi, Y., Matsunaga, S., Ohkubo, T., Robinson, C.V., Fukui, K.
  • Journal Title: Biochem.J. 436 Pages: 101-112
  • Peer Reviewed
• [Journal Article] The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles. (2011)
  • Author(s): Ma, N., Matsunaga, S., Morimoto, A., Sakashita, G., Urano, T., Uchiyama, S., Fukui, K.
  • Journal Title: J.Cell Sci.*These authors equally contributed to this work 124 Pages: 394-404
  • NAID: 120006994997
  • Peer Reviewed
• [Journal Article] Sequence Analysis of the Genome of an Oil-Bearing Tree, Jatropha curcas L. (2011)
  • Author(s): Sato, S., Hirakawa, H., Isobe, S., Fukai, E., Watanabe, A., Kato, M., Kawashima, K., Minami, C., Muraki, A., Nakazaki, N., Takahashi, C., Nakayama, S., Kishida, Y., Kohara, M., Yamada, M., Tsuruoka, H., Sasamoto, S., Tabata, S., Aizu, T., Toyoda, A., Shin-I, T., Minakuchi, Y., Kohara, Y., Fujiyama, A., Tsuchimoto, S., Kajiyama, S., Makigano, E., Ohmido, N., Shibagaki, N., Cartagena, J. A., Wada, N., Kohinata, T., Atefeh, A., Yuasa, S., Matsunaga, S., Fukui, K.
  • Journal Title: DNA Res. 18 Pages: 65-76
  • NAID: 120006994998
  • Peer Reviewed
• [Journal Article] Sequence analysis of the genome of an oil-bearing tree, Jatropha curcas (2011)
  • Author(s): Sato, S., et al.
  • Journal Title: DNA Res. Volume: 18 Pages: 65-76
  • Peer Reviewed
• [Journal Article] The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles. (2011)
  • Author(s): Ma, N., Matsunaga, S., Morimoto, A., et al.
  • Journal Title: J.Cell Sci. Volume: 124 Pages: 394-404
  • NAID: 120006994997
  • Peer Reviewed
• [Journal Article] Assembly states of the nucleosome assembly protein 1(NAP-1) revealed by sedimentation velocity and non-denaturing mass spectrometry. (2011)
  • Author(s): Noda, M., et al.
  • Journal Title: Biochem.J. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] The chromosome peripheral proteins play an active role in chromosome dynamics. (2010)
  • Author(s): Matsunaga, S., Fukui, K.
  • Journal Title: BioMol.Con. 1 Pages: 157-164
  • NAID: 120006980199
  • Peer Reviewed
• [Journal Article] Sex chromosome evolution revealed by physical mapping of SlAP3X/Y in the dioecious plant, Silene latifolia. (2010)
  • Author(s): Nishiyama, R., Ishii, K., Kifune, E., Kazama, Y., Nishihara, K., Matsunaga, S., Shinozaki, K., Kawano, S.
  • Journal Title: Cytologia 75 Pages: 319-325
  • NAID: 10027435000
  • Peer Reviewed
• [Journal Article] The MAP kinase MPK4 is required for cytokinesis in Arabidopsis thaliana. (2010)
  • Author(s): Kosetsu, K., Matsunaga, S., Nakagami, H., Colcombet, J., Sasabe, M., Soyano, T., Takahashi, Y., Hirt, H., Machida, Y.
  • Journal Title: Plant Cell 22 Pages: 3778-3790
  • Peer Reviewed
• [Journal Article] The middle region of an HP1-binding protein, HP1-BP74, associates with linker DNA at the entry/exit site of nucleosomal DNA. (2010)
  • Author(s): Hayashihara, K., Uchiyama, S., Shimamoto. S., Kobayashi, S., Tomschik, M., Wakamatsu, H., No, D., Sugahara, H., Hori, N., Noda, M., Ohkubo, T., Zlatanova, J., Matsunaga, S., Fukui, K.
  • Journal Title: J.Biol.Chem. 285 Pages: 6498-6507
  • NAID: 120006995000
  • Peer Reviewed
• [Journal Article] The MAP kinase MPK4 is required for cytokinesis in Arabidopsis thaliana. (2010)
  • Author(s): Kosetsu, K., Matsunaga, S., et al.
  • Journal Title: Plant Cell Volume: 22 Pages: 3778-3790
  • Peer Reviewed
• [Journal Article] Sex chromosome evolution revealed by physical mapping of SIAP3X/Y in the dioecious plant, Silene latifolia. (2010)
  • Author(s): Nishiyama, R., et al.
  • Journal Title: Cytologia Volume: 75 Pages: 319-325
  • Peer Reviewed
• [Journal Article] The middle region of an HPl-binding protein, HP1-BP74, as sociates with linker DNA at the entry/exit site of nucleosomal DNA. (2010)
  • Author(s): Hayashihara K, Uchiyama S, Shimamoto S, Kobayashi S, Tomschik M, Wakamatsu H, No D, Sugahara H, Hori N, Noda M, Ohkubo T, Zlatanova J, Matsunaga S, Fukui K.
  • Journal Title: J.Biol.Chem. 285 Pages: 6498-6507
  • Peer Reviewed
• [Journal Article] Junk DNA promotes sex chromosome evolution. (2009)
  • Author(s): Matsunaga, S.
  • Journal Title: Heredity 102 Pages: 525-526
  • Peer Reviewed
• [Journal Article] A nucleolar protein RRS1 contributes to chromosome congression. (2009)
  • Author(s): Gambe, A.E., Matsunaga, S., Takata, H., Ono-Maniwa, R., Baba, A., Uchiyama, S., Fukui, K.
  • Journal Title: FEBS Lett. 583 Pages: 1651-1956
  • Peer Reviewed
• [Journal Article] Intracellular manipulation by nonlinear microscopy. (2009)
  • Author(s): Watanabe, W., Matsunaga, S., Fukui, K., Itoh, K.
  • Journal Title: J.Innov.Opt.Health Sci. 2 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Junk DNA promotes sex chromosome evolution. (2009)
  • Author(s): Matsunaga, S
  • Journal Title: Heredity 102 Pages: 525-526
  • Peer Reviewed
• [Journal Article] Intracellular manipulation by nonlinear microscopy. (2009)
  • Author(s): Watanabe, W, Matsunaga, S, Fukui, K, Itoh, K
  • Journal Title: J.Innov.Opt.Health Sci. 2 Pages: 1-8
  • Peer Reviewed
• [Journal Article] A nucleolar protein RRS1 contributes to chromosome congression. (2009)
  • Author(s): Gambe, A.E., Matsunaga, S., Takata, H., Ono-Maniwa, R., Baba, A., Uchiyama S., Fukui K.
  • Journal Title: FEBS Lett. 583 Pages: 1951-1956
  • Peer Reviewed
• [Journal Article] 染色体動態-染色体の動き、動物と植物の共通点と違い (2009)
  • Author(s): 栗原大輔、松永幸大
  • Journal Title: 遺伝 63 Pages: 55-60
• [Journal Article] Nucleophosmin is required for chromosome congression, proper mitotic spindle formation, and kinetochore-microtubule attachment in HeLa cells. (2008)
  • Author(s): Amin, M.A., Matsunaga, S., Uchiyama, S., Fukui, K.
  • Journal Title: FEBS Lett. 582 Pages: 3839-3844
  • Peer Reviewed
• [Journal Article] Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cells. (2008)
  • Author(s): Amin, M.A., Matsunaga, S., Uchiyama, S., Fukui, K.
  • Journal Title: Biochem.J. 415 Pages: 345-351
  • NAID: 120006995003
  • Peer Reviewed
• [Journal Article] Live cell imaging reveals plant Aurora kinase has dual roles during mitosis. (2008)
  • Author(s): Kurihara, D., Matsunaga, S., Uchiyama, S., Fukui, K.
  • Journal Title: Plant Cell Physiol. 49 Pages: 1256-1291
  • NAID: 10027333174
  • Peer Reviewed
• [Journal Article] In vivo manipulation of fluorescently-labelled organelles in living cells by multi-photon excitation. (2008)
  • Author(s): Watanabe, W., Matsunaga, S., Higashi, T., Fukui, K., Itoh, K.
  • Journal Title: J.Biomed.Opt. 13 Pages: 31213-31221
  • NAID: 120006980201
  • Peer Reviewed
• [Journal Article] The use of repetitive DNA in cytogenetic studies of plant sex chromosomes. Cytogenet. (2008)
  • Author(s): Kazama, Y., Matsunaga, S.
  • Journal Title: Genet.Res. 120 Pages: 247-254
  • Peer Reviewed
• [Journal Article] Nucleophosmin is required for chromosome congression, proper mitot ic spindle formation, and kinetochore-microtubule attachment in HeLa cells. (2008)
  • Author(s): Am in MA, Matsunaga S, Uchiyama S, Fukui K
  • Journal Title: FEBS Letters 582 Pages: 3839-3844
  • Peer Reviewed
• [Journal Article] Depletion of nucleophosmin leads to distortion of nucleolarand nuclear structures in HeLa cells. (2008)
  • Author(s): Amin MA, Matsunaga S, Uchiyama S, Fukui K
  • Journal Title: Biochem. J. 415 Pages: 345-351
  • Peer Reviewed
• [Journal Article] In vivo manipulation of fluorescently-labelled organelles inliving cells by multi-photon excitation. (2008)
  • Author(s): Watanabe W, Matsunaga S, Higashi T, Fukui K, Itoh K
  • Journal Title: J. Biomed. Opt. 415 Pages: 31213-31221
  • Peer Reviewed
• [Journal Article] Live cell imaging reveals plant Aurora kinase has dualroles during mitosis. (2008)
  • Author(s): Kurihara D., Matsunaga, S., Uchiyama, S., Fukui, K.
  • Journal Title: Plant Cell Physiol. 49 Pages: 1256-1291
  • Peer Reviewed
• [Journal Article] The use of repetitive DNA in cytogenetic studies of plant sexchromosomes. (2008)
  • Author(s): Kazama, Y., Matsunaga, S.
  • Journal Title: Cytogenet. Genet. Res. 120 Pages: 247-254
  • Peer Reviewed
• [Presentation] Functional analysis of a chromosomal protein responsible for sister chromatid cohesion. (2010)
  • Author(s): 松永幸大
  • Organizer: 第4回アジア染色体コロキウム国際会議
  • Place of Presentation: 中国・北京
  • Year and Date: 2010-10-11
• [Presentation] 中部大学・春日井 (2010)
  • Author(s): 松永幸大、栗原大輔、大村知広、浅田拓也、福井希一
  • Organizer: 日本植物学会
  • Year and Date: 2010-09-09
• [Presentation] イメージングによるオーロラキナーゼ・ノックダウン個体の表現型解析 (2010)
  • Author(s): 松永幸大、栗原大輔、大村知広、浅田拓也、福井希一
  • Organizer: 日本植物学会
  • Place of Presentation: 中部大学・春日井
  • Year and Date: 2010-09-09
• [Presentation] (2009)
  • Author(s): Sachihiro Matsunaga, Hideaki Takata, Akihiro Morimoto, Daisuke Kurihara, Susumu Uchiyama, Kiichi Fukui
  • Organizer: 分子生物学会
  • Place of Presentation: パシフィコ国際会議場(横浜)
  • Year and Date: 2009-12-11
• [Presentation] 福井希一核内スキャホールドタンパク質による微小管ダイナミクス制御 (2009)
  • Author(s): 松永幸大、馬楠、森本晃弘、内山進
  • Organizer: 第81回日本遺伝学会大会
  • Place of Presentation: 信州大学理学部(長野県松本市)
  • Year and Date: 2009-09-16
• [Presentation] 核内スキャホールドタンパク質による微小管ダイナミクス制御 (2009)
  • Author(s): 松永幸大、馬楠、森本晃弘、内山進、福井希一
  • Organizer: 第81回日本遺伝学会大会
  • Place of Presentation: 信州大学理学部(長野県松本市)
  • Year and Date: 2009-09-16
• [Presentation] 染色体イメージングによる細胞分裂解析 (2008)
  • Author(s): 松永幸大
  • Organizer: 日本植物学会第72回大会
  • Place of Presentation: 高知大学朝倉キャンパス
  • Year and Date: 2008-09-26
• [Presentation] 染色体動態を制御するタンパク質の解析 (2008)
  • Author(s): 松永幸大
  • Organizer: 日本環境変異原学会
  • Place of Presentation: 青山学院大学青山キャンパス
  • Year and Date: 2008-05-31
• [Book] Molecular Biology and Evolution of the Plant Genome(edited by Greilhuber, J.,Leitch, I., Wendel, J. and Dolezel, J.)(The organisation of genomic DNA in mitotic chromosomes: a novel view.) (Heidelberg, Germany) (2011)
  • Author(s): Takata, H., Matsunaga S., Maeshima, K.
  • Publisher: Springer Verlag in press
• [Book] New Insights on Plant Sex Chromosomes(edited by Navajas R.)(The role of repetitive sequences in plant sex chromosome evolution.)(New York, USA) (2011)
  • Author(s): Kazama, Y.,
  • Publisher: Nova Scientific Publisher in press
• [Book] 「遺伝情報の格納庫」PhotoBook 植物細胞の世界(西村幹夫監修) (2010)
  • Author(s): 藤本聡、松永幸大
  • Publisher: 化学同人
• [Book] 「遺伝情報の格納庫」PhotoBook植物細胞の世界 (2010)
  • Author(s): 藤本聡、松永幸大
  • Publisher: 化学同人(印刷中)
• [Book] 「植物のかたち・核」植物の辞典(岩槻邦男、矢原徹一、和田正三他編集) (2009)
  • Author(s): 松永幸大
  • Publisher: 朝倉書店
• [Book] 細胞(基礎分子生物学シリーズ3) (2008)
  • Author(s): 黒岩常祥、三角修己、高野博嘉、伊藤竜一、松永幸大
  • Total Pages: 164
  • Publisher: 朝倉書店
• [Remarks]
  • URL: http://www.bio.eng.osaka-u.ac.jp/cl/matsunaga.html