| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2008: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Research Abstract |
In this study, we determined the crystal structure of a C-terminal fragment of FlgJ, which has a muramidase activity, and revealed the molecular mechanism of the peptidoglican hydrolysis. The active site structure and the overall folding of FlgJ resemble those of hen egg white lysozyme. Interestingly, the activity of FlgJ is strongly inhibited by a very small amount of cation. We reconstituted distal rod by adding rod proteins to the purified flagellar hook. Moreover, we elucidated the interaction between the rod and FlgD, the hook cap protein.
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