Structural bases of spliceosome indepemdent splicing
| Project/Area Number |
20370038
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
KOJIMA Chojiro Nara Institute of Science and Technology, 蛋白質研究所, 准教授 (50333563)
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| Co-Investigator(Kenkyū-buntansha) |
HAKOSHIMA Toshio 奈良先端科学技術大学院大学, 情報科学研究科, 教授 (00164773)
OHKI Izuro 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (80418574)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2009: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2008: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | 構造生物学 / NMR / RNA / タンパク質 / 分子認識 |
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| Research Abstract |
Structural bases of spliceosome indepemdent splicing found on IRE1 pathway in ER stress response, are investigated. Specifically an RNase domain of yeast Ire1p and its target mRNAs are applied to structural and interaction study. Based on NMR data, secondary structures of the RNase domain and the target mRNAs are determined. Cleavage experiments and NMR titration experiments of many mutants have revealed the site-specific RNase reaction mechanism of Ire1p.
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Report
(4 results)
Research Products
(61 results)
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[Journal Article] 14-3-3 proteins act as intracellular receptors for rice Hd3a florigen.2011
Author(s)
Ken-ichiro Taoka, Izuru Ohki, Hiroyuki Tsuji, Kyoko Furuita, Kokoro Hayashi, Tomoko Yanase, Midori Yamaguchi, Chika Nakashima, Yekti Asih Purwestri, Shojiro Tamaki, Yuka Ogaki, Chihiro Shimada, Atsushi Nakagawa, Chojiro Kojima, Ko Shimamoto
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Journal Title
Nature, accepted in principle
Related Report
Peer Reviewed
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[Journal Article] The structure of the N-terminal regulatory domain of a plant NADPH oxidase and its functional implications.2010
Author(s)
Takashi Oda, Hiroshi Hashimoto, Naoyuki Kuwabara, Satoko Akashi, Kokoro Hayashi, Chojiro Kojima, Hann Ling Wong, Tsutomu Kawasaki, Ko Shimamoto, Mamoru Sato, Toshiyuki Shimizu
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Journal Title
J.Biol.Chem. 285
Pages: 1435-1445
Related Report
Peer Reviewed
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[Presentation] NMR study of HAC1 mRNA recognized by endoplasmic reticulum stress sensor Ire1p(NMR を用いた小胞体ストレスセンサータンパク質Ire1pの基質認識機構の解析)2009
Author(s)
Ikumi Kawahara, JunGoo Jee, Rikou Tanaka, Toshiyuki Kohno, Toshio Hakoshima, Yusuke Imagawa, Kenji Kohno, Yoshiyuki Tanaka, Chojiro Kojima
Organizer
The 32nd Annual Meeting of the Molecular Biology Society of Japan(日本分子生物学会年会)
Place of Presentation
Pacifico Yokohama, Yokohama, Japan
Related Report
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[Presentation] NMR studies of HAC1 mRNA2009
Author(s)
Ikumi Kawahara, Kaichiro Haruta, Chojiro Kojima, Yoshiyuki Tanaka
Organizer
The 6th International Symposium on Nucleic Acids Chemistry 2009
Place of Presentation
Takayama Public Cultural Hall, Takayama, Japan
Related Report
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