| Budget Amount *help |
¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2009: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2008: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Research Abstract |
Structural bases of spliceosome indepemdent splicing found on IRE1 pathway in ER stress response, are investigated. Specifically an RNase domain of yeast Ire1p and its target mRNAs are applied to structural and interaction study. Based on NMR data, secondary structures of the RNase domain and the target mRNAs are determined. Cleavage experiments and NMR titration experiments of many mutants have revealed the site-specific RNase reaction mechanism of Ire1p.
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