X-ray crystallographic analysis of microsomal prostaglandin E2synthase1
| Project/Area Number |
20370042
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
AGO Hideo The Institute of Physical and Chemical Research, 宮野構造生物物理研究室, 専任研究員 (70360477)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2009: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | アラキドン酸カスケード / 膜タンパク質 / X線結晶構造解析 / プロスタグランジンE2 / 膜蛋白質 / 結晶化 / 膜蛋白 / X線結晶構造 / 蛋白質改変 / 酵素活性 / モノクローナル抗体 |
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| Research Abstract |
Human membrane protein microsomal prostaglandin E2 synthase 1(mPGES1) was overexpressed using Schizosaccharomyces pombe. The purification method was established. The recombinant mPGES1 was free from the post-translational modification except for the deletion of the N-terminal methionine residue, and had a higher specific activity. The recombinant mPGES1 was crystallized in the two crystallization conditions.
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Report
(4 results)
Research Products
(11 results)
