Molecular mechanisms of basement membrane recognition by integrins

• Project/Area Number: 20370046
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Osaka University
• Principal Investigator: SEKIGUCHI Kiyotoshi Osaka University, 蛋白質研究所, 教授 (50187845)
• Co-Investigator(Kenkyū-buntansha): YAMADA Masashi 大阪大学, 蛋白質研究所, 助教 (90304055)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000); Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2009: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2008: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: 基底膜 / 細胞外マトリックス / インテグリン / ラミニン / ネフロネクチン / テトラスパニン

Research Abstract

Affinity and specificity of interactions between the integrin family of cell adhesion receptors and their ligands of basement membranes are quantitatively explored with an emphasis on laminins, nephronectin, and other Arg-Gly-Asp motif-containing proteins. A novel protocol for collectively visualizing integrin ligands on tissue specimens has also been developed.

Research Products

• [Journal Article] The basement membrane of hair follicle stem cells is a muscle cell niche. (2011)
  • Author(s): Fujiwara, H., Ferreira, M., Donati, G., Marciano, D.K., Linton, J.M., Sato, Y., Hartner, A., Sekiguchi, K., Reichardt, L.F., Watt, F.M.
  • Journal Title: Cell 144 Pages: 577-589
• [Journal Article] Activin A binds to perlecan through its pro-region that has heparin/heparan sulfate-binding activity. (2010)
  • Author(s): Li, S., Shimono, C., Norioka, N., Nakano, I., Okubo, T., Yagi, Y., Hayashi, M., Sato, Y., Fujisaki H., Hattori, S., Sugiura, N., Kimata, K., Sekiguchi, K.
  • Journal Title: J.Biol.Chem. 285 Pages: 36645-36655
  • NAID: 120006585490
• [Journal Article] Identification of genes expressed during hair follicle induction. (2010)
  • Author(s): Kiyozumi, D., Osada, A., Sugimoto N., Weber, C.N., Ono, Y., Imai, T., Okada, A., Sekiguchi, K.
  • Journal Title: J.Dermatol. 10 Pages: 1346-8138
  • NAID: 10031147066
• [Journal Article] Direct binding of the EGF-like domain of neuregulin-1 to integrins avb3 and a6b4 is involved in neuregulin-1/ErbB signaling. (2010)
  • Author(s): Ieguchi, K., Fujita, M., Ma, Z., Davari, P., Taniguchi, Y., Sekiguchi, K., Wang, B., Takada, Y., Takada, Y.
  • Journal Title: J.Biol.Chem. 285 Pages: 31388-31398
• [Journal Article] Kif26b, a kinesin family gene, regulates adhesion of the embryonic kidney mesenchyme. (2010)
  • Author(s): Uchiyama, Y., Sakaguchi, M., Terabayashi, T., Inenaga, T., Inoue, S., Kobayashi, C., Oshima, N., Kiyonari, H., Nakagata, N., Sato, Y., Sekiguchi, K., Miki, H., Fujimura, S., Tanaka, S., Nishinakamura, R.
  • Journal Title: Proc.Natl.Acad.Sci.USA. 107 Pages: 9240-9245
• [Journal Article] Identification and characterization of nCLP2, a novel Clq family protein expressed in the central nervous system. (2010)
  • Author(s): Shimono, C., Manabe, R., Yamada, T., Fukuda, S., Kawai, J., Furutani, Y., Tsutsui, K., Ikenaka, K., Hayashizaki, Y., Sekiguchi, K.
  • Journal Title: J.Biochem. 151 Pages: 565-579
• [Journal Article] ADAMTSL-6 is a novel extracellular matrix protein that binds to fibrillin-1 and promotes fibrillin-1 fibril formation. (2010)
  • Author(s): Tsutsui, K., Manabe, R., Yamada, T., Nakano, I., Oguri, Y., Keene, D.R., Sengle G., Sakai, L.Y., Sekiguchi, K.
  • Journal Title: J.Biol.Chem. 285 Pages: 4870-4882
  • NAID: 120006585492
• [Journal Article] ADAMTSL-6 is a novel extracellular matrix protein that binds to fibrillin-1 and promotes fibrillin-1 fibril formation. (2010)
  • Author(s): Tsutsui, K., et al.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 4870-4882
  • Peer Reviewed
• [Journal Article] Identification of genes expressed during hair follicle induction. (2010)
  • Author(s): Kiyozumi, D., et al.
  • Journal Title: J.Dermatol. Volume: 10 Pages: 1346-8138
  • Peer Reviewed
• [Journal Article] Activin a binds to perlecan through its pro-region that has heparin/heparan sulfate-binding activity. (2010)
  • Author(s): Li, S., et al.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 36645-36655
  • Peer Reviewed
• [Journal Article] Identification and characterization of nCLP2, a novel Clq family protein expressed in the central nervous system. (2010)
  • Author(s): Shimono, C., et al.
  • Journal Title: J.Biochem. (in press)
  • Peer Reviewed
• [Journal Article] Molecular basis of the recognition of nephronectin by integrin a8b1. (2009)
  • Author(s): Sato, Y., Uemura, T., Morimitsu, K., Sato-Nishiuchi, R., Manabe, R., Takagi, J., Yamada, M., Sekiguchi, K.
  • Journal Title: J.Biol.Chem. 284 Pages: 14524-14536
• [Journal Article] The carboxyl-terminal region of laminin b chains modulates the integrin-binding affinities of laminins. (2009)
  • Author(s): Taniguchi, Y., Ido, H., Sanzen, N., Hayashi, M., Sato-Nishiuchi, R., Futaki, S., Sekiguchi, K.
  • Journal Title: J.Biol.Chem. 284 Pages: 7820-7831
• [Journal Article] Anti-laminin gamma-1 pemphigoid. (2009)
  • Author(s): Dainichi, T., Kurono, S., Ohyama, B., Ishii, N., Sanzen, N., Hayashi, M., Shimono, C., Taniguchi, Y., Koga, H., Karashima, T., Yasumoto, S., Zillikens, D., Sekiguchi, K., Hashimoto, T.
  • Journal Title: Proc.Natl.Acad.Sci.USA. 106 Pages: 2800-2805
• [Journal Article] Molecular basis of the recognition of nephronectin by integrin α8β1. (2009)
  • Author(s): Sato, Y., et al.
  • Journal Title: J.Biol.Chem. 284 Pages: 7820-7831
  • Peer Reviewed
• [Journal Article] ADAMTSL-6 is a novel extracellular matrix protein that binds to fibrillin-1 and promotes fibrillin-1 fibril formation. (2009)
  • Author(s): Tsutsui, K., et al.
  • Journal Title: J.Biol.Chem. 285 Pages: 4870-4882
  • Peer Reviewed
• [Journal Article] The C-terminmal region of lamin β chains modulates the lntegrinbinding affinities of laminins. (2009)
  • Author(s): Taniguchi, Y., et al.
  • Journal Title: J. Biol. Chem. 284(in press)
  • Peer Reviewed
• [Journal Article] Recombinant human laminin isoforms can support the undifferentiated growth of human embryonic stem cells. (2008)
  • Author(s): Miyazaki, T., Futaki, S., Hasegawa, K., Kawasaki, M., Sanzen, N., Hayashi, M., Kawase, E., Sekiguchi, K., Nakatsuji, N., Suemori, H.
  • Journal Title: Biochem.Biophys.Res.Commun. 375 Pages: 27-32
• [Journal Article] Probing the interaction of tetraspanin CD151 with integrin a3b1 using a panel of monoclonal antibodies with distinct reactivities toward the CD151-integrin a3b1 complex. (2008)
  • Author(s): Yamada, M., Tamura Y., Sanzen, N., Sato-Nishiuchi, R., Hasegawa, H., Ashman, L.K., Rubinstein, E., Yanez-Mo, M., Sanchez-Madrid, F., Sekiguchi, K.
  • Journal Title: Biochem.J. 415 Pages: 417-427
• [Journal Article] The tetraspanin CD151 regulates cell morphology and intracellular signaling on laminin-511. (2008)
  • Author(s): Yamada, M., Sumida, Y., Fujibayashi, A., Fukaguchi, K., Sanzen, N., Sato-Nishiuchi, R., Sekiguchi, K.
  • Journal Title: FEBS J. 275 Pages: 3335-3351
• [Journal Article] Laminin isoforms containing the g3 chain are unable to bind to integrins due to the absence of the glutamic acid residue conserved in the C-terminal regions of the g1 and g2 chains. (2008)
  • Author(s): Ido, H., Ito, S., Taniguchi, Y., Hayashi, M., Sato-Nishiuchi, R., Sanzen, N., Hayashi, Y., Futaki, S., Sekiguchi, K.
  • Journal Title: J.Biol.Chem. 283 Pages: 28149-28157
• [Journal Article] Laminin-511 is an epithelial message promoting dermal papilla development and function during early hair morphogenesis. (2008)
  • Author(s): Gao, J., DeRouen, M.C., Chen, C.H., Nguyen, M., Nguyen, N.T., Ido, H., Harada, K., Sekiguchi, K., Morgan, B.A., Miner, J.H., Oro, A.E., Marinkovich, M.P.
  • Journal Title: Genes Dev. 22 Pages: 2111-2124
  • NAID: 120006626429
• [Journal Article] Transcriptome-based systematic identification of extracellular matrix proteins. (2008)
  • Author(s): Manabe, R., Tsutsui, K., Fukuda, T., Yamada, T., Kimura, M., Nakano, I., Shimono, C., Sanzen, N., Furutani Y., Fukuda T., Oguri, Y., Shimamoto, K., Kiyozumi, D., Sato, Y., Sado, Y., Senoo, H., Yamashina, S., Fukuda, S., Kawai, J., Sugiura, N., Kimata, K., Hayashizaki, Y., Sekiguchi, K.
  • Journal Title: Proc.Natl.Acad.Sci.USA. 105 Pages: 12849-12854
• [Journal Article] The tetraspanin CD151 regulates cell morphology and intracellularsignaling on laminin-511. (2008)
  • Author(s): Yamada, M., et al.
  • Journal Title: FEBS J. 275 Pages: 3335-3351
  • Peer Reviewed
• [Journal Article] Isoforms containing the(c)3. chain are unable to bind to Integrins due to the absence of the glutamic. acid residue conserved in the C-terminal regions of the γ1 and γ2 chains. (2008)
  • Author(s): Ido, H., et al. Laminin
  • Journal Title: J. Biol. Chem. 283 Pages: 28149-28157
  • Peer Reviewed
• [Presentation] Activin A binds to perlecan through its pro-region that has heparin/heparan sulfate-binding activity. (2010)
  • Author(s): Sekiguchi, K.
  • Organizer: Biennial Meeting of the American Society for Matrix Biology
  • Place of Presentation: Charleston, SC, USA
  • Year and Date: 2010-10-26
• [Presentation] Activin A binds to perlecan through its pro region that has heparin/heparan sulfate binding activity. (2010)
  • Author(s): Sekiguchi, K.
  • Organizer: Biennial Meeting of the American Society for Matrix Biology
  • Place of Presentation: チャールストン(アメリカ)(招待講演)
  • Year and Date: 2010-10-26
• [Presentation] Untangling the complexities of basement membranes. (2010)
  • Author(s): Sekiguchi, K.
  • Organizer: Shriners Research Center Seminar, Oregon Health & Science University
  • Place of Presentation: Portland, OR, USA
  • Year and Date: 2010-10-22
• [Presentation] Molecular dissection of the integrin binding sites of laminins. (2010)
  • Author(s): Sekiguchi, K.
  • Organizer: International Symposium on Basement Membranes in Tissue Development and Regeneration
  • Place of Presentation: Nashville, TN, USA
  • Year and Date: 2010-07-08
• [Presentation] 基底膜分子QBRICKが持つインテグリン結合活性の生理機能評価 (2009)
  • Author(s): 難口征雅, 他7名
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸ポートピアホテル
  • Year and Date: 2009-10-22
• [Presentation] Customization of the basement membrane during embryonic development. (2009)
  • Author(s): Sekiguchi, K.
  • Organizer: 8th Pan-Pacific Connective Tissue Societies Symposium
  • Place of Presentation: Yokosuka, Japan
  • Year and Date: 2009-06-04
• [Presentation] Customization of the basement membrane during embryonic development. Gordon Research Conference on Fibronectin (2009)
  • Author(s): Sekiguchi, K.
  • Organizer: Integrins and Related Molecules
  • Place of Presentation: Ventura, CA, USA
  • Year and Date: 2009-02-04
• [Presentation] Molecular basis of the recognition of nephronectin by integrin a8b1. Gordon Research Conference on Fibronectin (2009)
  • Author(s): Sato, Y., Uemura, T., Morimitsu, K., Sato-Nishiuchi, R., Manabe, R., Takagi, J., Yamada, M., Sekiguchi, K.
  • Organizer: Integrins and Related Molecules
  • Place of Presentation: Ventura, CA, USA
• [Presentation] The carboxyl-terminal region of laminin b chains modulates the integrin-binding affinities of laminins. (2009)
  • Author(s): Taniguchi, Y., Ido, H., Sanzen, N., Hayashi, M., Nishiuchi, R., Futaki, S., Sekiguchi, K.
  • Organizer: 8th Pan-Pacific Connective Tissue Societies Symposium
  • Place of Presentation: Yokosuka, Japan
• [Presentation] Molecular basis of the recognition of nephronectin by integrin a8β1. (2009)
  • Author(s): Sato, Y., et al.
  • Organizer: Gordon Research Conference on FibronectinIntegrins and Related Molecules
  • Place of Presentation: Ventura, CA, USA
• [Presentation] Molecular basis of basement membrane recognition by integrins. (2008)
  • Author(s): Sekiguchi, K.
  • Organizer: Gordon Research Conference on Basement Membranes
  • Place of Presentation: Biddeford, ME, USA
  • Year and Date: 2008-06-23
• [Presentation] The carboxyl-terminal region of laminin b chains modulates the integrin binding activity of laminins. (2008)
  • Author(s): Taniguchi, Y., Ido, H., Sanzen, N., Hayashi, M., Nakano, I., Nishiuchi, R., Futaki, S., Sekiguchi, K.
  • Organizer: Gordon Research Conference on Basement Membranes
  • Place of Presentation: Biddeford, ME, USA
• [Presentation] Diversification of the basement membrane composition during early stages of mouse embryogenesis. (2008)
  • Author(s): Futaki, S., Nakano, I., Manabe, R., Tsutsui, K., Sanzen, N., Sado, Y., Sekiguchi, K.
  • Organizer: Gordon Research Conference on Basement Membranes
  • Place of Presentation: Biddeford, ME, USA
• [Presentation] Construction of Mouse Basement Membrane Bodymap, an immunohistochemical image database of basement membrane proteins in mouse embryos. (2008)
  • Author(s): Tsutsui, K., Manabe, R., Sanzen, N., Nakano, I., Sado, Y., Futaki, S., Sekiguchi, K.
  • Organizer: Gordon Research Conference on Basement Membranes
  • Place of Presentation: Biddeford, ME, USA
• [Remarks] ホームページ等
  • URL: http://www.protein.osaka-u.ac.jp/chemistry/
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/chemistry/
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/chemistry/
• [Patent(Industrial Property Rights)] ヒト多能性幹細胞用培養基材およびその応用 (2009)
  • Inventor(s): 関口清俊、二木杉子、谿口征雅、中辻憲夫、宮崎隆道、末盛博文、川瀬栄八郎
  • Industrial Property Rights Holder: 国立大学法人大阪大学・国立大学法人京都大学
  • Industrial Property Number: 2009-235348
  • Filing Date: 2009-10-08