Structural and functional study of enzymes which are related to central nervous system disorders with muscle deficiency
| Project/Area Number |
20370053
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | High Energy Accelerator Research Organization |
|---|
Principal Investigator |
KATO Ryuichi High Energy Accelerator Research Organization, 物質構造科学研究所, 准教授 (50240833)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ENDO Tamao 地独東京都健康長寿医療センター, 東京都健康長寿医療センター研究所, 研究部長 (30168827)
KAWASAKI Masato 高エネルギー加速器研究機構, 物質構造科学研究所, 助教 (00342600)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2009: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2008: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
|
|---|
| Keywords | 糖鎖生物学 / 糖鎖修飾 / 筋ジストロフィー / ジストログライカン / X線結晶構造解析 |
|---|
| Research Abstract |
We elucidated PomT1 and T2 require sugar modification on their proteins for the activity. We also showed that mechanism of PomT1-T2 complex formation is different between human and yeast but similar between human and zebra-fish. Over expression and purification of PomGnT1 protein for X-ray structural determination was successful by wheat germ cell free expression system. Over expression and purification of fukutin and FKRP proteins were also successful by E. coli expression system.
|
Report
(4 results)
Research Products
(22 results)
