Structural and functional study of enzymes which are related to central nervous system disorders with muscle deficiency

• Project/Area Number: 20370053
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: High Energy Accelerator Research Organization
• Principal Investigator: KATO Ryuichi High Energy Accelerator Research Organization, 物質構造科学研究所, 准教授 (50240833)
• Co-Investigator(Kenkyū-buntansha): ENDO Tamao 地独東京都健康長寿医療センター, 東京都健康長寿医療センター研究所, 研究部長 (30168827) ; KAWASAKI Masato 高エネルギー加速器研究機構, 物質構造科学研究所, 助教 (00342600)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000); Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2009: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2008: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: 糖鎖生物学 / 糖鎖修飾 / 筋ジストロフィー / ジストログライカン / X線結晶構造解析

Research Abstract

We elucidated PomT1 and T2 require sugar modification on their proteins for the activity. We also showed that mechanism of PomT1-T2 complex formation is different between human and yeast but similar between human and zebra-fish. Over expression and purification of PomGnT1 protein for X-ray structural determination was successful by wheat germ cell free expression system. Over expression and purification of fukutin and FKRP proteins were also successful by E. coli expression system.

Research Products

• [Journal Article] Role of N-glycans in maintaining the activity of protein O-mannosyltransferases POMT1 and POMT2. (2010)
  • Author(s): Hiroshi Manya, Keiko Akasaka-Manya, Ai Nakajima, Masao Kawakita, Tamao Endo
  • Journal Title: J.Biochem. 147(3) Pages: 337-344
  • NAID: 10027874335
• [Journal Article] Protein O-mannosylation is necessary for normal embryonic development in zebrafish. (2010)
  • Author(s): Eriko Avsar-Ban, Hisayoshi Ishikawa, Hiroshi Manya, Masatoki Watanabe, Shinichi Akiyama, Hideo Miyake, Tamao Endo, Yutaka Tamaru
  • Journal Title: Glycobiology 20(9) Pages: 1089-102
• [Journal Article] Protein O-mannosylation is necessary for normal embryonic development in zebrafish (2010)
  • Author(s): Eriko Avsar-Ban
  • Journal Title: Glycobiology Volume: 20 Pages: 1089-1102
  • Peer Reviewed
• [Journal Article] Role of N-glycans in maintaining the activity of protein O-mannosyltransferases POMT1 and POMT2 (2010)
  • Author(s): Hiroshi Manya
  • Journal Title: J.Biochem. 147 Pages: 337-344
  • Peer Reviewed
• [Journal Article] Protein O-mannosylation is necessary for normal embryonic development in zebrafish (2010)
  • Author(s): Eriko Avsar-Ban
  • Journal Title: Glycobiology (印刷中)
  • Peer Reviewed
• [Presentation] ヒトO-マンノース転移酵素におけるN型糖鎖修飾の解析 (2010)
  • Author(s): 萬谷博、赤阪-萬谷啓子、遠藤玉夫
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
• [Presentation] Role of N-glycans on protein O-mannosyltransferases POMT1 and POMT2. (2010)
  • Author(s): Hiroshi Manya, Keiko Akasaka-Manya, Tamao Endo
  • Organizer: XXV International Carbohydrate Symposium
  • Place of Presentation: Tokyo
• [Presentation] Investigation the role of protein O-mannosylation during development. (2010)
  • Author(s): Tamao Endo, Eriko Avsar-Ban, Hiroshi Manya, Yutaka Tamaru
  • Organizer: Annual Conference of the Society for Glycobiology
  • Place of Presentation: St Pete Beach, FL, USA
• [Presentation] POMGnT1の基質特異性の解析 (2010)
  • Author(s): 赤阪啓子、萬谷博、水野真盛、稲津敏行、遠藤玉夫
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] ゼブラフィッシュ初期発生過程においてO-マンノース型糖鎖修飾が必要である (2010)
  • Author(s): アヴシャル-坂恵利子、石川文啓、萬谷博、渡部正利喜、秋山真一、三宅英雄、遠藤玉夫、田丸浩
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] POMGnT1の基質特異性の解析 (2010)
  • Author(s): 赤阪啓子
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
• [Presentation] ゼブラフィッシュ初期発生過程においてO-マンノース型糖鎖修飾が必要である (2010)
  • Author(s): アヴシャル-坂恵利子
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸
• [Presentation] 構造生物学研究センター報告 (2010)
  • Author(s): 加藤龍一
  • Organizer: 第27回PFシンポジウム
  • Place of Presentation: 茨城
• [Presentation] ゼブラフィッシュにおけるPOMTおよびPOMGnT1によるO-mannosylation (2009)
  • Author(s): 坂恵利子、萬谷博、三宅英雄、遠藤玉夫、田丸浩
  • Organizer: 日本農芸化学会2009年度大会
• [Presentation] Role on N-glycans on protein O-mannosyltransferases POMT1 and POMT2. (2009)
  • Author(s): Hiroshi Manya, Keiko Akasaka-Manya, Tamao Endo
  • Organizer: Austria/Japan Seminar on Comparative Glycobiology and Developmental Biology
  • Place of Presentation: Hayama, Japan
• [Presentation] O-マンノース型糖鎖修飾と病態 (2009)
  • Author(s): 遠藤玉夫,萬谷博
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] ゼブラフィッシュにおけるPOMTおよびPOMGnT1によるO-mannosylation (2009)
  • Author(s): 坂恵利子
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
• [Presentation] 神経移動障害を伴う先天性筋ジストロフィー (2008)
  • Author(s): 遠藤玉夫、萬谷博、赤阪-萬谷啓子
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
• [Presentation] O-マンノース転移酵素におけるN型糖鎖の役割 (2008)
  • Author(s): 萬谷博、赤阪-萬谷啓子、遠藤玉夫
  • Organizer: 第28回日本糖質学会年会
  • Place of Presentation: つくば
• [Presentation] O-マンノース転移酵素におけるN型糖鎖の役割 (2008)
  • Author(s): 萬谷博、赤阪-萬谷啓子、遠藤玉夫
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] ジストログリカンの糖鎖修飾と筋ジストロフィー (2008)
  • Author(s): 遠藤玉夫、萬谷博
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] 培養細胞を用いたゼブラフィッシュPOMT遺伝子の発現とその酵素活性の検討 (2008)
  • Author(s): 坂恵利子、萬谷博、遠藤玉夫、田丸浩
  • Organizer: 第81回日本生化学会大会
  • Place of Presentation: 神戸