Cooperation between multiple heads in moving dynein molecule

• Project/Area Number: 20370057
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: The University of Tokyo
• Principal Investigator: TOYOSHIMA Yoko The University of Tokyo, 大学院・総合文化研究科, 教授 (40158043)
• Co-Investigator(Renkei-kenkyūsha): EDAMATSU Masaki 東京大学, 大学院・総合文化研究科, 助教 (60251328)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000); Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2009: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2008: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
• Keywords: ダイニン / 微小管 / 分子モーター / zincシート / プロセッシビティ / 歩行モデル / プロセッシビティー / 複数分子 / 分子間協同性 / ピンセット / 1分子計測 / 破断力

Research Abstract

Cytoplasmic dynein is a two-headed microtubule (MT) motor protein. While kinesin moves by a hand-over-hand mechanism, the dynein walking mechanism is unknown. As the dynein motor domain is much larger than the step size, the two heads of dynein are thought to use multiple protofilaments of MT. Here we report dynein and kinesin motility on zinc-induced tubulin polymer (zinc-sheet), where only one protofilament can be used by motor proteins. Dynein and kinesin moved unidirectionally on a zinc-sheet at a similar velocity as MTs, demonstrating that both dynein and kinesin can walk on a single protofilament and multiple rows of parallel protofilaments are not essential for motility. These findings provide a clue as to how dynein uses the two huge motor domains for molecular walking.

Research Products

• [Journal Article] Functional Dissection of LIS1 and NDEL1 Towards Understanding the Molecular Mechanisms of Cytoplasmic Dynein Regulation (2011)
  • Author(s): Torisawa T., Nakayama A., Furuta K., Yamada M., Hirotsune, S., Toyoshima Y.Y.
  • Journal Title: J.Biol.Chem. 286 Pages: 1959-1965
• [Journal Article] Functional Dissection of LISL and NDELl Towards Understanding the Molecular Mechanisms of Cytoplasmic Dynein Regulation. (2011)
  • Author(s): Torisawa T., Nakayama A., Furuta K., Yamada M., Hirotsune, S.and Tovoshima Y.Y.
  • Journal Title: J. Biol.Chem. Volume: 286 Pages: 1959-1965
  • Peer Reviewed
• [Journal Article] Nucleotide-dependent behavior of single molecules of cytoplasmic dynein on microtubules in vitro (2010)
  • Author(s): Miura, M., Matsubara, A., Kobayashi, T., Edamatsu, M., Toyoshima, Y.Y.
  • Journal Title: FEBS Lett. 584 Pages: 2351-2355
• [Journal Article] Nucleotide-dependent behavior of single molecules of cytoplasmic dynein on microtubules in vitro. (2010)
  • Author(s): Miura, M., Matsubara, A., Kobayashi, T., Edamatsu, M., Toyoshima, Y.Y.
  • Journal Title: FEBS Lett. Volume: 584 Pages: 2351-2355
  • Peer Reviewed
• [Journal Article] Inhibition of cytoplasmic dynein activity by CRMP-2 through a direct interaction. (2009)
  • Author(s): Arimura, N., Hattori, A., Kimura, T., Nakamura, S., Funahashi, Y., Hirotsune, S., Furuta, K., Toyoshima, Y.Y., Kaibuchi, K.
  • Journal Title: J Neurochem. 111 Pages: 380-390
• [Journal Article] Inhibition of cytoplasmic dynein activity by CRMP-2 through a direct interaction. (2009)
  • Author(s): Arimura, N., K., Toyoshima, Y.Y., et al.
  • Journal Title: J Neurochem. 111 Pages: 380-390
  • Peer Reviewed
• [Journal Article] モータータンパク質 (2009)
  • Author(s): 豊島陽子
  • Journal Title: 分子生物学イラストレイテッド 改訂第3版 Pages: 187-191
• [Journal Article] Diffusion and directed movement: In vitro motile properties of fission yeast kinesin-14 Pkl1. (2008)
  • Author(s): Furuta, K., Toyoshima, Y.Y.
  • Journal Title: J.Biol.Chem. 283 Pages: 36465-36473
• [Journal Article] LIS1 and NDEL1 coordinate the plus-end-directed transport of cytoplasmic dynein. (2008)
  • Author(s): Yamada, M.Toba, S., Yoshida, Y., Haratani, K., Mori, D., Yano, Y., Mimori-Kiyosue, Y., Nakamura, T., Ito,mK., Fushiki, S.,Setou, M., Whnshaw-Boris, A., Torisawa, T., Toyoshima, Y.Y., Hirotsune, S.
  • Journal Title: EMBO J. 27 Pages: 2471-2483
• [Journal Article] Direction and speed of microtubule movements driven by kinesin motors arranged on catchin thick filaments. (2008)
  • Author(s): Taba, T., Edamatsu, M., Toba, S., Shibata, K., Imafuku, Y., Toyoshima, Y.Y., Tawada, K., Yamada, A.
  • Journal Title: Cell Motility and the Cytoskeleton 65 Pages: 816-826
• [Journal Article] The dynein stalk head, the microtubule binding-domain of dynein: NMR assignment and ligand binding. (2008)
  • Author(s): Shimizu, Y., Kato, Y., Morii, H., Edamatsu, M., Toyoshima, Y.Y, Tanokura, M.
  • Journal Title: J.Biomol.NMR 41 Pages: 89-96
• [Journal Article] Minus-end-directed motor Ncd exhibits processive movement that is enhanced by microtubule bundling in vitro (2008)
  • Author(s): Furuta, K., Toyoshima, Y.Y.
  • Journal Title: Current Biology 18 Pages: 152-157
• [Journal Article] Minimus-end-directect motor Ncd exhibits processive movement that is enhanced by microtubule bundling in vitro (2008)
  • Author(s): Furuta, K. and Toyoshima, Y. Y.
  • Journal Title: Current Biology 18 Pages: 152-157
  • Peer Reviewed
• [Journal Article] Direction and speed of microtubule movements driven by kinesin motorsarranged on catchin thick filaments (2008)
  • Author(s): Taba, T., Edamatsu, M., Toba, S., Shibata, K, Imafuku, Y., Toyoshima, YY Tawada K and Yamada A
  • Journal Title: Cell Motihty and the Cytoskeleton 65 Pages: 816-826
  • Peer Reviewed
• [Journal Article] Diffusion and directed movernent: In vitro motile properties of fission yeastkinesin-14 Pkl1 (2008)
  • Author(s): Furuta, K. and Toyoshima, Y. Y.
  • Journal Title: J. Biol. Chem. 2835 Pages: 36465-36473
  • Peer Reviewed
• [Presentation] Functional dissection of LIS1 and NDEL1 for understanding the molecular mechanism of cytoplasmic dyenin regulation. (2011)
  • Author(s): 鳥澤嵩征、渡辺裕多、中山明子、古田健也、山田正巳、広常真治、豊島陽子
  • Organizer: 米国Biophysical Society 55th Annual Meeting、Baltimore
  • Place of Presentation: 東京大学、大阪市立大学
  • Year and Date: 2011-03-08
• [Presentation] Single protofilmant is enough to support unidirectional walking of cytoplasmic dynein (2011)
  • Author(s): 柴田桂太朗、三浦未知、渡辺裕多、西村敦子、枝松正樹、豊島陽子
  • Organizer: 米国Biophysical Society 55th Annual Meeting, Baltimore
  • Place of Presentation: 東京大学
  • Year and Date: 2011-03-08
• [Presentation] Structural analysis of cytoplasmic dynein tail domain (2011)
  • Author(s): 渡辺裕多、北井敏幸、小林琢也、村山尚、高萩隆行、豊島陽子
  • Organizer: 米国Biophysical Society 55th Annual Meeting, Baltimore
  • Place of Presentation: 東京大学、広島大学、順天堂大学
  • Year and Date: 2011-03-08
• [Presentation] Reconstitusion of collective transport by defind numbers of kinesin-1 and kinesin-14, two opposing motor proteins (2011)
  • Author(s): 古田健也、豊島陽子、小島寛明、大岩和弘
  • Organizer: 米国Biophysical Society 55th Annual Meeting, Baltimore
  • Place of Presentation: 通信総合研究所、東京大学
  • Year and Date: 2011-03-08
• [Presentation] Single protofilament is enough to support unideirectional walking of cytoplasmic dyenin. (2011)
  • Author(s): Keitaro Shibata , Michi Miura, Yuta Watanabe, Atsuko Nishimura, Masaki Edamatsu, Yoko Y. Tovoshima.
  • Organizer: Biophysical Society 55th Annual Meeting
  • Place of Presentation: Baltimore, USA
  • Year and Date: 2011-03-07
• [Presentation] Structural analysis of cytoplasmic dynein tail domain. (2011)
  • Author(s): Watanabe, Toshiyuki Kitai, Takuya Kobayashi, Takashi Murayama, Takayuki Takahagi, Yoko Y. Tovoshima
  • Organizer: Biophysical Society 55th Annual Meeting
  • Place of Presentation: Baltimore, USA
  • Year and Date: 2011-03-07
• [Presentation] Functional dissection of LISl and NDELl for understanding the molecular mechanism of dynein regulation. (2011)
  • Author(s): Takayuki Torisawa, Akiko Nakayama, Kenya Furuta, Masami Yamada, Shinji Hirotsune, Yoko Y.Toyoshima
  • Organizer: Biophysical Society 55th Annual Meeting
  • Place of Presentation: Baltimore, USA
  • Year and Date: 2011-03-07
• [Presentation] Reconstitusion of collective transport by defined numbers of kinesin-1 and kinesin-14. (2011)
  • Author(s): Ken'ya Furuta, Yoko Y.Toyoshima, Hiroaki Kojima, Kazuhiro Oiwa
  • Organizer: Biophysical Society 55th Annual Meeting
  • Place of Presentation: Baltimore, USA
  • Year and Date: 2011-03-07
• [Presentation] Diffusive movement of a single-molecvule mammalian cytoplasmic dynein (2010)
  • Author(s): 鳥澤嵩征、市川宗厳、小林琢也、村山尚、豊島陽子
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 仙台(東京大学、順天堂大学)
  • Year and Date: 2010-09-22
• [Presentation] Single protofilmant is enough to support unidirectional walking of cytoplasmic dynein (2010)
  • Author(s): 柴田桂太朗、三浦未知、渡辺裕多、西村敦子、枝松正樹、豊島陽子
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 仙台(東京大学)
  • Year and Date: 2010-09-20
• [Presentation] Development of a new mechod to synthesize functional gold nanoparticles for labeling a protein (2010)
  • Author(s): 北井敏幸、渡辺裕多、豊島陽子、小林琢也、村山尚、坂上浩之、鈴木一志、高萩隆行
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 仙台(広島大学、東京大学、順天堂大学)
  • Year and Date: 2010-09-20
• [Presentation] Electron microscopic observation of cytoplasmic dynein using gold nanoparticles (2010)
  • Author(s): 渡辺裕多、北井敏幸、小林琢也、村山尚、高萩隆行、豊島陽子
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 仙台(東京大学、広島大学、順天堂大学)
  • Year and Date: 2010-09-20
• [Presentation] Dynamic behavior of dynein-dynactin in living mammalian cells visualized by multifunctional GFP tag (2010)
  • Author(s): 村山尚、小林琢也、渡辺裕多、豊島陽子、北井敏幸、高萩隆行
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 仙台(順天堂大学、東京大学、広島大学)
  • Year and Date: 2010-09-20
• [Presentation] A sarucomere-mimetic gel : Gelation of astral-shpaled actin filaments with their plus end conncted on photopolymer beads by myosin filaments (2010)
  • Author(s): 井川泰二、塩澤雅人、毛利誠、成田真美子、星野文彦、渡辺修、枝松正樹、豊島陽子
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 仙台(豊田中央研究所、東京大学)
  • Year and Date: 2010-09-20
• [Presentation] Single protofilmant is enough to support unidirectional walking of cytoplasmic dynein. (2010)
  • Author(s): 柴田桂太朗、三浦未知、渡辺裕多、西村敦子、枝松正樹、豊島陽子
  • Organizer: 生物物理学会第53回年会
  • Place of Presentation: 仙台(東北大学以内キャンパス)
  • Year and Date: 2010-09-20
• [Presentation] Development of a new mechod to synthesize functional gold nanopartides for labeling a protein. (2010)
  • Author(s): 北井敏幸、渡辺裕多、豊島陽子、小林琢也、村山尚、坂上浩之、鈴木一志、高萩隆行
  • Organizer: 生物物理学会第53回年会
  • Place of Presentation: 仙台(東北大学以内キャンパス)
  • Year and Date: 2010-09-20
• [Presentation] Electron microscopic observation of cyto@lasmic dyneinusing gold nanoparticles. (2010)
  • Author(s): 渡辺裕多、北井敏幸、小林琢也、村山尚、高萩隆行、豊島陽子
  • Organizer: 生物物理学会第53回年会
  • Place of Presentation: 仙台(東北大学以内キャンパス)
  • Year and Date: 2010-09-20
• [Presentation] 生物物理学会第53回年会 (2010)
  • Author(s): 村山尚、小林琢也、渡辺裕多、豊島陽子、北井敏幸、高萩隆行
  • Organizer: Dynamic behavior of dynein-dynactin in living mammalian cells visualized by multifunctional GFP tag.
  • Place of Presentation: 仙台(東北大学以内キャンパス)
  • Year and Date: 2010-09-20
• [Presentation] Diffusive movement of a single-molecvule mammalian cytoplasmic dynein. (2010)
  • Author(s): 鳥澤嵩征、市川宗厳、小林琢也、村山尚、豊島陽子
  • Organizer: 生物物理学会第53回年会
  • Place of Presentation: 仙台(東北大学以内キャンパス)
  • Year and Date: 2010-09-20
• [Presentation] A sarucomere-mimetic gel : Gelation of astral-shpaled actin filaments with their plus end conncted on photopolymer beads by myosin filaments. (2010)
  • Author(s): 井川泰二、篠沢正弘、毛利誠、成田真美子、星野文彦、渡辺修枝松正樹、豊島陽子
  • Organizer: 生物理学会第53回年会
  • Place of Presentation: 仙台(東北大学以内キャンパス)
  • Year and Date: 2010-09-20
• [Presentation] Regulation and Cordination of Cytoplasmic Dynein Revealed by in vitro Study (2009)
  • Author(s): Toyoshima, Y.Y.
  • Organizer: International Workshop Dynein 2009
  • Place of Presentation: 神戸・オルビスホール
  • Year and Date: 2009-11-03
• [Presentation] Dynein in the coordinated system. (2009)
  • Author(s): 豊島陽子
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: 徳島(東京大学)
  • Year and Date: 2009-11-01
• [Presentation] Motility of single molecules of kinesin and cytoplasmic dynein in the presence of many molecules of motor proteins on microtubules. (2009)
  • Author(s): 柴田桂太朗、古田健也、枝松正樹、豊島陽子
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: 徳島(東京大学、通信総合研究所)
  • Year and Date: 2009-10-30
• [Presentation] DNA-templated assembly of multiple motor protein complexes. (2009)
  • Author(s): 古田健也、豊島陽子、小嶋寛明
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: 徳島(通信総合研究所、東京大学)
  • Year and Date: 2009-10-30
• [Presentation] LIS1 regulates dynein motility in a dimerization-dependent manner. (2009)
  • Author(s): 鳥澤嵩征、山田正巳、広常真治、豊島陽子
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: 徳島(東京大学、大阪市立大学)
  • Year and Date: 2009-10-30
• [Presentation] Force production and processive movement by a defined number of nonprocessive motor proteins. (2008)
  • Author(s): 古田健也、豊島陽子
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡(東京大学)
  • Year and Date: 2008-12-05
• [Presentation] Properties of dynein motiliy analyzed with recombinant molecules. (2008)
  • Author(s): 豊島陽子
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡(東京大学)
  • Year and Date: 2008-12-04
• [Presentation] Unbinding force of cytoplasmic dynein-microtubule interaction. (2008)
  • Author(s): 岩崎聡、枝松正樹、Nguyen HoaAnh、古田健也、樋口秀雄、豊島陽子
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡(東京大学)
  • Year and Date: 2008-12-03
• [Presentation] Molecular mechanism of the regulation of cytoplasmic dynein motility by LIS1. (2008)
  • Author(s): 鳥澤嵩征、古田健也、枝松正樹、山田正巳、広常真治、豊島陽子
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡(東京大学、大阪市立大学)
  • Year and Date: 2008-12-03
• [Presentation] Motility assay of single dynein molecules on immobilized microtubules on azopolymer. (2008)
  • Author(s): 柴田桂太郎、古田健也、枝松正樹、井川泰二、塩澤雅人、成田真美子、渡辺修、豊島陽子
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡(東京大学、豊田中央研究所)
  • Year and Date: 2008-12-03
• [Book] 東京化学同人生物学辞典(ダイニン、ミオシン、チューブリンなど20項目) (2010)
  • Author(s): 豊島陽子(分担執筆)
• [Book] 生物学辞典(ATP合成酵素ほか20項目) (2010)
  • Author(s): 豊島陽子(分担執筆、共著者約400名)
  • Total Pages: 1615
  • Publisher: 東京化学同人
• [Book] モータータンパク質(分子生物学イラストレイテッド(改訂第3版)) (2009)
  • Author(s): 豊島陽子(分担執筆)
  • Publisher: 羊土社
• [Remarks] ホームページ等
  • URL: http://bio.c.u-tokyo.ac.jp/labs/toyoshim/