Elucidation and regulation of signal-propagation mechanisms in receptor proteins
| Project/Area Number |
20370059
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Kyoto University |
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Principal Investigator |
IMAMOTO Yasushi Kyoto University, 大学院・理学研究科, 准教授 (80263200)
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| Co-Investigator(Kenkyū-buntansha) |
SHICHIDA Yoshinori 京都大学, 大学院・理学研究科, 教授 (60127090)
YAMASHITA Takahiro 京都大学, 大学院・理学研究科, 助教 (50378535)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000)
Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2008: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
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| Keywords | ロドプシン / PYP / 構造変化 / 蛋白質 / 活性化 / アゾベンゼン / フォトクロミズム / 蛋白質工学 / ペプチド / αヘリックス / 立体構造 / G蛋白質共役型受容体 |
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| Research Abstract |
Proteins change their conformations in response to various kinds of stimuli. To reveal the intramolecular interaction that induces the protein conformational change, the amino acid residues which are responsible for the protein conformational changes of photoreceptor proteins in bacteria and animal retina were identified. Based on these findings, the protein conformation was mechanically controlled by a linker whose length is changed by photo-isomerization to regulate the activity of the protein.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Structural effects on the ultrafast photoisomerization of photoactive yellow protein. ransient absorption spectroscopy of two point mutants.2009
Author(s)
Changenet-Barret. P., Plaza, P., Martin, M.M., Chosrowjan, H., Taniguchi, S., Mataga, N., Imamoto, Y., Kataoka, M.
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Journal Title
J.Phys.Chem. C113
Pages: 11605-11613
Related Report
Peer Reviewed
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[Journal Article] G protein subtype specificity of rhodopsin intermediates metarhodopsin Ib and metarhodopsin II.2009
Author(s)
Morizumi, T., Kimata, N., Terakita, A., Imamoto, Y., Yamashita, T., Shichida, Y.
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Journal Title
Photochem. Photobiol. 85
Pages: 57-62
Related Report
Peer Reviewed
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[Journal Article] The photoreaction of the photoactive yellow protein domain in the light sensor histidine kinase Ppr is influenced by the C-terminal domains.2008
Author(s)
Kamikubo, H., Koyama, T., Hayashi, M., Shirai, K., Yamazaki, Y., Imamoto, Y., Kataoka, M.
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Journal Title
Photochem. Photobiol. 84
Pages: 895-902
Related Report
Peer Reviewed
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