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Structural study of the torque-generating unit in the bacterial flagellar motor

Research Project

Project/Area Number 20370064
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

YONEKURA Koji  独立行政法人理化学研究所, 米倉生体機構研究室, 准主任研究員 (50346144)

Co-Investigator(Kenkyū-buntansha) MAKI-YONEKURA Saori  独立行政法人理化学研究所, タンパク質結晶構造解析研究グループ, 研究員 (20513386)
Project Period (FY) 2008 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2008: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Keywords構造生物学 / イオンチャネル / べん毛モーター / 電子顕微鏡 / X 線結晶構造解析 / X線結晶構造解析
Research Abstract

The bacterial flagellum is a rotary motor powered by the electrochemical potential of specific ions across the cytoplasmic membrane. There is little information about its working mechanism of this biological motor, the information which could be useful for biological science and nano-technology. In Vibrio, an inner membrane complex, PomAB, converts Na+ flux into rotation of the flagellum. We have been studying the structure of PomAB by X-ray crystallography and cryo-electron microscopy to elucidate the working mechanism of this energy conversion system.

Report

(7 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report   Self-evaluation Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (61 results)

All 2013 2012 2011 2010 2009 2008 Other

All Journal Article (15 results) (of which Peer Reviewed: 13 results) Presentation (43 results) (of which Invited: 3 results) Remarks (3 results)

  • [Journal Article] 非結晶粒子のコヒーレントX線回折イメージング2012

    • Author(s)
      中迫雅由、高山裕貴、苙口友隆、白濱圭也、関口 優希、山本雅貴、米倉功治、引間孝明、眞木さお り、高橋幸生、鈴木明大、松永幸大、加藤翔一、星 貴彦
    • Journal Title

      レーザー研究

      Volume: 40 Pages: 680-686

    • NAID

      10031004094

    • Related Report
      2012 Annual Research Report
  • [Journal Article] X線自由電子レーザーを用いた非結晶粒子のコヒーレントX線回折イメージング実験2012

    • Author(s)
      中迫雅由、高山裕貴、苙口友隆、白濱圭也、関口 優希、山本雅貴、米倉功治、引間孝明、眞木さお り、高橋幸生、鈴木明大、松永幸大、加藤翔一、星 貴彦
    • Journal Title

      月刊OPTRONICS

      Volume: 31 Pages: 101-106

    • Related Report
      2012 Annual Research Report
  • [Journal Article] The structure of the flagellar motor protein complex PomAB: Implications for the torque-generating conformation2011

    • Author(s)
      Koji Yonekura, Saori Maki-Yonekura, Michio Homma
    • Journal Title

      J. Bacteriol

      Volume: 193 Pages: 3863-3870

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 生体超分子の高分解能構造解析のための電子線イメージングの条件2011

    • Author(s)
      米倉功治
    • Journal Title

      顕微鏡

      Volume: 45 Pages: 243-249

    • NAID

      10031144710

    • Related Report
      2012 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The structure of the flagellar motor protein complex PomAB : Implications for the torque-generating conformation2011

    • Author(s)
      Koji Yonekura, Saori Maki-Yonekura, Michio Homma
    • Journal Title

      J.Bacteriol.

      Volume: 193 Issue: 15 Pages: 3863-3870

    • DOI

      10.1128/jb.05021-11

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 生体超分子の高分解能構造解析のための電子線イメージングの条件2011

    • Author(s)
      米倉功治
    • Journal Title

      顕微鏡 45

      Pages: 243-249

    • NAID

      10031144710

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Conformational change of flagellin for polymorphic supercoi ling of the flagellar filament2010

    • Author(s)
      Saori Maki-Yonekura, Koji Yonekura, Keiichi Namba
    • Journal Title

      Nat. Struct. Mol. Biol

      Volume: 17 Pages: 417-422

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 低温電子顕微鏡法による生体超分子構造の解析 --- 電子顕微鏡はどこまでタンパク質の立体構造に迫れるか---2010

    • Author(s)
      米倉功治
    • Journal Title

      日本結晶学会誌

      Volume: 52 Pages: 56-61

    • NAID

      10026107867

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Conformational change of flagellin for polymorphic supercoi ling of the flagellar filament2010

    • Author(s)
      Saori Maki-Yonekura, Koji Yonekura
    • Journal Title

      Nat.Struct.Mol.Biol. 17

      Pages: 417-422

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] 低温電子顕微鏡法による生体超分子構造の解析-電子顕微鏡はどこまでタンパク質の立体構造に迫れるか-2010

    • Author(s)
      米倉功治
    • Journal Title

      日本結晶学会誌 52

      Pages: 56-61

    • NAID

      10026107867

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Conformational change of flagellin for polymorphic supercoiling of the flagellar filament2010

    • Author(s)
      Saori Maki-Yonekura, Koji Yonekura, Keiichi Namba
    • Journal Title

      Nat.Struct.Mol.Biol. (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 低温電子顕微鏡法による生体超分子構造の解析 -電子顕微鏡はどこまでタンパク質の立体構造に迫れるか-2010

    • Author(s)
      米倉功治
    • Journal Title

      日本結晶学会誌 52(印刷中)

    • NAID

      10026107867

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electron digital imaging towards high-resolution structure analysis of biological macromolecules2008

    • Author(s)
      Saori Maki-Yonekura, Koji Yonekura
    • Journal Title

      Microsc.Microanal.

      Volume: 14 Pages: 362-369

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Electron digital imaging towards high-resolution structure analysis of biological macromolecules2008

    • Author(s)
      Saori Maki-Yonekura, Koji Yonekura,
    • Journal Title

      Microsc.Microanal. 14

      Pages: 362-369

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] The structure of the flagellar motor protein complex PomAB : Implications for the torque-generating conformation

    • Author(s)
      Koji Yonekura, Saori Maki-Yonekura, Michio Homma
    • Journal Title

      Accepted to J.Bacteriol.

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Presentation] Cryo-Electron Microscopy of Biological Macromolecular Structures2013

    • Author(s)
      K. Yonekura, S. Maki-Yonekura
    • Organizer
      4-th France-Japan Joint Seminar "Imaging of spatiotemporal hierarchies in living cells - an overview of dynamics from molecules to cells -"
    • Place of Presentation
      RIKEN SPring-8 Center (Hyogo)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Structure Analysis of Biological Macromolecular Complexes by Electron- and X-rays2013

    • Author(s)
      K. Yonekura
    • Organizer
      The 5th Biennale RIKEN Joint Retreat "Behavior - from Molecules, Cell to Organisms -"
    • Place of Presentation
      YAMAHA Resort Tsumagoi (Shizuoka)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Electron Crystallography of 3D Protein Crystals2013

    • Author(s)
      K. Yonekura, C. Toyoshima
    • Organizer
      Nagoya Symposium - Frontiers in Structural Physiology
    • Place of Presentation
      Nagoya University (Nagoya)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 電子線とX線による生体超分子構造の解析2013

    • Author(s)
      米倉功治
    • Organizer
      平成24年度「分子システム研究」成果報告会
    • Place of Presentation
      理研和光(埼玉)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 低温電子顕微鏡法による生体超分子複合体の構造解析2012

    • Author(s)
      米倉功治、眞木さおり
    • Organizer
      第13回医学生物学電子顕微鏡シンポジウム
    • Year and Date
      2012-11-24
    • Related Report
      2012 Final Research Report
  • [Presentation] 低温電子顕微鏡法による生体超分子複合体の構造解析2012

    • Author(s)
      米倉功治、眞木さおり
    • Organizer
      第13回医学生物学電子顕微鏡学
    • Place of Presentation
      チサンホテル新大阪(大阪)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 細菌のイオン共役型エネルギー供与体の構造基盤研究2012

    • Author(s)
      渡邊真宏
    • Organizer
      分子システム研究」第1回春合宿
    • Place of Presentation
      浜名湖ロイヤルホテル(静岡)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 生体の作動機構をより高い分解能で“見る”2012

    • Author(s)
      米倉功治
    • Organizer
      分子システム研究」第1回春合宿
    • Place of Presentation
      浜名湖ロイヤルホテル(静岡)
    • Related Report
      2012 Annual Research Report
  • [Presentation] イオン共役型エネルギー供与体ExbB/ExbDおよびTonB/ExbB複合体の結晶化構造解析2012

    • Author(s)
      渡邊真宏、米倉功治
    • Organizer
      第12回蛋白質科学会
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Purification and crystallization of bacterial ion-coupled energizing protein ExbB2012

    • Author(s)
      M. Watanabe, K. Yonekura
    • Organizer
      The 2012 Annual Meeting of American Crystallographic Association
    • Place of Presentation
      Boston(MA), USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] 細菌のイオン共役型エネルギー供与体の構造基盤研究2012

    • Author(s)
      渡邊真宏、米倉功治
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡(福岡)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 生体超分子構造の解析2011

    • Author(s)
      米倉功治
    • Organizer
      細菌学会中部支部会
    • Place of Presentation
      名古屋大学、名古屋(招待講演)
    • Year and Date
      2011-10-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] Correlative imaging with CXDI and cryo-EM2011

    • Author(s)
      Koji Yonekura, Saori Maki-Yonekura
    • Organizer
      The 4th International Workshop on FEL Science : Science Challenges of XFEL
    • Place of Presentation
      Queensland, Australia(招待講演)
    • Year and Date
      2011-08-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] Structure analysis of biological macromolecules2011

    • Author(s)
      米倉功治
    • Organizer
      The 1st Annual Meeting for Whole-Organism Science Society Joint Meeting with The 10th Annual Meeting of Structural-Biological Whole Cell Project of Thermus thermophilus HB8
    • Place of Presentation
      理研、播磨(招待講演)
    • Year and Date
      2011-08-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] Cryo-electron microscopy of biological macromolecules2011

    • Author(s)
      Koji Yonekura
    • Organizer
      Joint workshop with Wageningen University and SPring-8
    • Place of Presentation
      RIKEN, Harima(招待講演)
    • Year and Date
      2011-04-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Cryo-electron microscopy of biological macromolecules2011

    • Author(s)
      米倉功治、眞木さおり
    • Organizer
      NHRI workshop
    • Place of Presentation
      新竹、台湾
    • Year and Date
      2011-02-14
    • Related Report
      2010 Annual Research Report
  • [Presentation] Cryo-electron microscopy of biological macromolecules2011

    • Author(s)
      Koji Yonekura, Saori Maki-Yonekura
    • Organizer
      Workshop on structural biology
    • Place of Presentation
      Tainan, Taiwan
    • Year and Date
      2011-02-13
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] Cryo-electron microscopy of biological macromolecules2011

    • Author(s)
      米倉功治、眞木さおり
    • Organizer
      Workshop on structural biology
    • Place of Presentation
      台南、台湾
    • Year and Date
      2011-02-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] Cryo-electron microscopy of biological macromolecular structures2011

    • Author(s)
      米倉功治、眞木さおり
    • Organizer
      OIST seminar
    • Place of Presentation
      沖縄 OIST
    • Year and Date
      2011-01-14
    • Related Report
      2010 Annual Research Report
  • [Presentation] Electron crystallography in biological sciences2010

    • Author(s)
      Koji Yonekura
    • Organizer
      The satellite meeting, the 10^(th) conference of the Asian crystallographic association, Daejeon
    • Place of Presentation
      Korea
    • Year and Date
      2010-10-29
    • Related Report
      2012 Final Research Report
  • [Presentation] Electron crystallography in biological sciences2010

    • Author(s)
      Koji Yonekura
    • Organizer
      The satellite meeting, the 10^<th> conference of the Asian crystallographic association
    • Place of Presentation
      Daejeon, Korea
    • Year and Date
      2010-10-29
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] Electron crystallography in biological sciences2010

    • Author(s)
      米倉功治
    • Organizer
      The satellite meeting, the 10^<th> conference of the Asian crystallographic association
    • Place of Presentation
      KBSI, Daejeon, Korea
    • Year and Date
      2010-10-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] Correlative Imaging---CXDI and Cryo-EM---2010

    • Author(s)
      米倉功治、眞木さおり
    • Organizer
      The 3rd Workshop on FEL Science : "Emerging X-ray Applications in Biological Systems-II"
    • Place of Presentation
      北海道 北湯沢
    • Year and Date
      2010-10-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] 低温電子顕微鏡法による生体超分子の構造解析2010

    • Author(s)
      米倉功治
    • Organizer
      蛋白質の機能-構造相関解明のための精密構造解析とその方法~水素原子から細胞まで~蛋白研セミナー
    • Place of Presentation
      大阪大学 吹田
    • Year and Date
      2010-10-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] 低温電子顕微鏡法による生体超分子構造解析2010

    • Author(s)
      米倉功治
    • Organizer
      ASI-Spring8生物系合同シンポジウム
    • Place of Presentation
      理研 和光市
    • Year and Date
      2010-09-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] Cryo-electrop microscopy and helical image reconstruction of biological materials2010

    • Author(s)
      眞木さおり、米倉功治
    • Organizer
      アジア若手シンポジウム、日本顕微鏡学会第66回学術講演会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-05-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] 極低温電子顕微鏡で細菌を『見る』2010

    • Author(s)
      米倉功治
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2010-03-28
    • Related Report
      2012 Final Research Report 2010 Self-evaluation Report
  • [Presentation] 極低温電子顕微鏡で細菌を『見る」2010

    • Author(s)
      米倉功治
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 低温電子顕微鏡法による生体分子構造解析の撮像条件について2010

    • Author(s)
      米倉功治
    • Organizer
      電顕技術開発若手研究部会 第1回ワークショップ
    • Place of Presentation
      熱田、名古屋
    • Year and Date
      2010-01-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] Cryo-electron microscopy of macromolecular structures2010

    • Author(s)
      米倉功治・米倉さおり
    • Organizer
      2010 RIKEN Conference
    • Place of Presentation
      理研 播磨
    • Year and Date
      2010-01-13
    • Related Report
      2009 Annual Research Report
  • [Presentation] 低温電子顕微鏡法の核膜系への応用2009

    • Author(s)
      米倉功治・米倉さおり
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] Bioimaging-Cryo-Electron Microscopy2009

    • Author(s)
      米倉功治
    • Organizer
      The Second Workshop on FEL Science : "Emerging X-ray Applications in Biological Systems"
    • Place of Presentation
      Kenting, Taiwan
    • Year and Date
      2009-12-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] 3D Electron Microscopy of Biological Macromolecular Structures2009

    • Author(s)
      米倉功治
    • Organizer
      Workshop on POSTECH-RIKEN Joint Research Program
    • Place of Presentation
      理研 播磨
    • Year and Date
      2009-10-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] Cryo-electron microscopy of biological macromolecular structures2009

    • Author(s)
      米倉功治
    • Organizer
      SEMINAIRE IBS
    • Place of Presentation
      IBS, Grenoble, France
    • Year and Date
      2009-09-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] High-resolution cryo-electron microscopy of biological macromolecular structures by helical reconstruction2009

    • Author(s)
      Koji Yonekura, Saori Maki-Yonekura
    • Organizer
      Microscopy Conference 2009 in Graz
    • Place of Presentation
      Graz, Austria
    • Year and Date
      2009-09-02
    • Related Report
      2012 Final Research Report 2010 Self-evaluation Report 2009 Annual Research Report
  • [Presentation] 低温電顕微鏡法による生体超分子構造の解析2009

    • Author(s)
      米倉功治・米倉さおり
    • Organizer
      高度好熱菌丸ごと一匹プロジェクト 第8回連携研究会
    • Place of Presentation
      理研 播磨
    • Year and Date
      2009-08-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] 放射光、電子顕微鏡で生命の不思議を"見る"2009

    • Author(s)
      米倉功治
    • Organizer
      平成21年度「高校生のためのサイエンス・サマーキャンプ」
    • Place of Presentation
      理研 播磨
    • Year and Date
      2009-08-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] Cryo-electron Microscopy of Biological Macromolecular Structures2009

    • Author(s)
      米倉功治
    • Organizer
      The 1^<st> Workshop on FEL Science "Coherent Intense X-rays in Physics and Biology"
    • Place of Presentation
      ロッテホテルチェジュ韓国
    • Year and Date
      2009-02-18
    • Related Report
      2008 Annual Research Report
  • [Presentation] 電子顕微鏡でモーター分子を見る2008

    • Author(s)
      米倉功治
    • Organizer
      日本生物物理学会 第46回年会
    • Place of Presentation
      福岡
    • Year and Date
      2008-12-04
    • Related Report
      2012 Final Research Report
  • [Presentation] 電子顕微鏡でモーター分子を見る2008

    • Author(s)
      米倉功治
    • Organizer
      日本生物物理学会第46回年会
    • Place of Presentation
      福岡
    • Year and Date
      2008-12-04
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] 電子顕微鏡でモーター分子を見る2008

    • Author(s)
      米倉功治
    • Organizer
      日本生物物理学会第46回年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2008-12-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] 低温電子顕微鏡法による生体超分子構造の解析2008

    • Author(s)
      米倉功治
    • Organizer
      分子研研究会「物質系と生体系での自己組織化-異分野融合的研究の新展開に向けて-」
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2008-08-08
    • Related Report
      2008 Annual Research Report
  • [Presentation] 低温電子顕微鏡法の生体膜系への応用2008

    • Author(s)
      米倉功治
    • Organizer
      平成20年蛋白研セミナー細胞機能制御システムとしての核膜研究〜基礎から臨床応用を目指して〜
    • Place of Presentation
      大阪大学コンベンションセンター
    • Year and Date
      2008-07-24
    • Related Report
      2008 Annual Research Report
  • [Remarks] 米倉功治、眞木さおり、2009 年(独)電子顕微鏡学会Ernst Ruska 賞受賞。PomAB 単粒子解析の論文(Yonekura et al.,2011, J. Bacteriol.)がハイライトとして紹介されたAmerican Society for Microbiology のMicrobe Magazine の記事へのリンク

    • URL

      http://www.microbemagazine.org/index.php?option=com_content&view=article&id=3857:gentlemen-start-your-prokaryotic-motors&catid=876:07-2011-journal-highlights&Itemid=1129

    • Related Report
      2012 Final Research Report
  • [Remarks] 米倉生体機構研究室

    • Related Report
      2012 Annual Research Report
  • [Remarks] 米倉功治、眞木さおり、2009年(独)電子顕微鏡学会Ernst Ruska賞受賞。

    • Related Report
      2010 Self-evaluation Report

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Published: 2008-04-01   Modified: 2019-07-29  

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