Structural study of the torque-generating unit in the bacterial flagellar motor

• Project/Area Number: 20370064
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: YONEKURA Koji 独立行政法人理化学研究所, 米倉生体機構研究室, 准主任研究員 (50346144)
• Co-Investigator(Kenkyū-buntansha): MAKI-YONEKURA Saori 独立行政法人理化学研究所, タンパク質結晶構造解析研究グループ, 研究員 (20513386)
• Project Period (FY): 2008 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2008: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
• Keywords: 構造生物学 / イオンチャネル / べん毛モーター / 電子顕微鏡 / X 線結晶構造解析 / X線結晶構造解析

Research Abstract

The bacterial flagellum is a rotary motor powered by the electrochemical potential of specific ions across the cytoplasmic membrane. There is little information about its working mechanism of this biological motor, the information which could be useful for biological science and nano-technology. In Vibrio, an inner membrane complex, PomAB, converts Na+ flux into rotation of the flagellum. We have been studying the structure of PomAB by X-ray crystallography and cryo-electron microscopy to elucidate the working mechanism of this energy conversion system.

Research Products

• [Journal Article] 非結晶粒子のコヒーレントＸ線回折イメージング (2012)
  • Author(s): 中迫雅由、高山裕貴、苙口友隆、白濱圭也、関口 優希、山本雅貴、米倉功治、引間孝明、眞木さお り、高橋幸生、鈴木明大、松永幸大、加藤翔一、星 貴彦
  • Journal Title: レーザー研究 Volume: 40 Pages: 680-686
  • NAID: 10031004094
• [Journal Article] X線自由電子レーザーを用いた非結晶粒子のコヒーレントX線回折イメージング実験 (2012)
  • Author(s): 中迫雅由、高山裕貴、苙口友隆、白濱圭也、関口 優希、山本雅貴、米倉功治、引間孝明、眞木さお り、高橋幸生、鈴木明大、松永幸大、加藤翔一、星 貴彦
  • Journal Title: 月刊OPTRONICS Volume: 31 Pages: 101-106
• [Journal Article] The structure of the flagellar motor protein complex PomAB: Implications for the torque-generating conformation (2011)
  • Author(s): Koji Yonekura, Saori Maki-Yonekura, Michio Homma
  • Journal Title: J. Bacteriol Volume: 193 Pages: 3863-3870
  • Peer Reviewed
• [Journal Article] 生体超分子の高分解能構造解析のための電子線イメージングの条件 (2011)
  • Author(s): 米倉功治
  • Journal Title: 顕微鏡 Volume: 45 Pages: 243-249
  • NAID: 10031144710
  • Peer Reviewed
• [Journal Article] The structure of the flagellar motor protein complex PomAB : Implications for the torque-generating conformation (2011)
  • Author(s): Koji Yonekura, Saori Maki-Yonekura, Michio Homma
  • Journal Title: J.Bacteriol. Volume: 193 Issue: 15 Pages: 3863-3870
  • DOI: 10.1128/jb.05021-11
  • Peer Reviewed
• [Journal Article] 生体超分子の高分解能構造解析のための電子線イメージングの条件 (2011)
  • Author(s): 米倉功治
  • Journal Title: 顕微鏡 45 Pages: 243-249
  • NAID: 10031144710
  • Peer Reviewed
• [Journal Article] Conformational change of flagellin for polymorphic supercoi ling of the flagellar filament (2010)
  • Author(s): Saori Maki-Yonekura, Koji Yonekura, Keiichi Namba
  • Journal Title: Nat. Struct. Mol. Biol Volume: 17 Pages: 417-422
  • Peer Reviewed
• [Journal Article] 低温電子顕微鏡法による生体超分子構造の解析 --- 電子顕微鏡はどこまでタンパク質の立体構造に迫れるか--- (2010)
  • Author(s): 米倉功治
  • Journal Title: 日本結晶学会誌 Volume: 52 Pages: 56-61
  • NAID: 10026107867
  • Peer Reviewed
• [Journal Article] Conformational change of flagellin for polymorphic supercoi ling of the flagellar filament (2010)
  • Author(s): Saori Maki-Yonekura, Koji Yonekura
  • Journal Title: Nat.Struct.Mol.Biol. 17 Pages: 417-422
  • Peer Reviewed
• [Journal Article] 低温電子顕微鏡法による生体超分子構造の解析-電子顕微鏡はどこまでタンパク質の立体構造に迫れるか- (2010)
  • Author(s): 米倉功治
  • Journal Title: 日本結晶学会誌 52 Pages: 56-61
  • NAID: 10026107867
  • Peer Reviewed
• [Journal Article] Conformational change of flagellin for polymorphic supercoiling of the flagellar filament (2010)
  • Author(s): Saori Maki-Yonekura, Koji Yonekura, Keiichi Namba
  • Journal Title: Nat.Struct.Mol.Biol. (In press)
  • Peer Reviewed
• [Journal Article] 低温電子顕微鏡法による生体超分子構造の解析 -電子顕微鏡はどこまでタンパク質の立体構造に迫れるか- (2010)
  • Author(s): 米倉功治
  • Journal Title: 日本結晶学会誌 52(印刷中)
  • NAID: 10026107867
  • Peer Reviewed
• [Journal Article] Electron digital imaging towards high-resolution structure analysis of biological macromolecules (2008)
  • Author(s): Saori Maki-Yonekura, Koji Yonekura
  • Journal Title: Microsc.Microanal. Volume: 14 Pages: 362-369
  • Peer Reviewed
• [Journal Article] Electron digital imaging towards high-resolution structure analysis of biological macromolecules (2008)
  • Author(s): Saori Maki-Yonekura, Koji Yonekura,
  • Journal Title: Microsc.Microanal. 14 Pages: 362-369
  • Peer Reviewed
• [Journal Article] The structure of the flagellar motor protein complex PomAB : Implications for the torque-generating conformation
  • Author(s): Koji Yonekura, Saori Maki-Yonekura, Michio Homma
  • Journal Title: Accepted to J.Bacteriol.
  • Peer Reviewed
• [Presentation] Cryo-Electron Microscopy of Biological Macromolecular Structures (2013)
  • Author(s): K. Yonekura, S. Maki-Yonekura
  • Organizer: 4-th France-Japan Joint Seminar "Imaging of spatiotemporal hierarchies in living cells - an overview of dynamics from molecules to cells -"
  • Place of Presentation: RIKEN SPring-8 Center (Hyogo)
  • Invited
• [Presentation] Structure Analysis of Biological Macromolecular Complexes by Electron- and X-rays (2013)
  • Author(s): K. Yonekura
  • Organizer: The 5th Biennale RIKEN Joint Retreat "Behavior - from Molecules, Cell to Organisms -"
  • Place of Presentation: YAMAHA Resort Tsumagoi (Shizuoka)
  • Invited
• [Presentation] Electron Crystallography of 3D Protein Crystals (2013)
  • Author(s): K. Yonekura, C. Toyoshima
  • Organizer: Nagoya Symposium - Frontiers in Structural Physiology
  • Place of Presentation: Nagoya University (Nagoya)
• [Presentation] 電子線とX線による生体超分子構造の解析 (2013)
  • Author(s): 米倉功治
  • Organizer: 平成24年度「分子システム研究」成果報告会
  • Place of Presentation: 理研和光（埼玉）
• [Presentation] 低温電子顕微鏡法による生体超分子複合体の構造解析 (2012)
  • Author(s): 米倉功治、眞木さおり
  • Organizer: 第13回医学生物学電子顕微鏡シンポジウム
  • Year and Date: 2012-11-24
• [Presentation] 低温電子顕微鏡法による生体超分子複合体の構造解析 (2012)
  • Author(s): 米倉功治、眞木さおり
  • Organizer: 第13回医学生物学電子顕微鏡学
  • Place of Presentation: チサンホテル新大阪（大阪）
  • Invited
• [Presentation] 細菌のイオン共役型エネルギー供与体の構造基盤研究 (2012)
  • Author(s): 渡邊真宏
  • Organizer: 分子システム研究」第1回春合宿
  • Place of Presentation: 浜名湖ロイヤルホテル（静岡）
• [Presentation] 生体の作動機構をより高い分解能で“見る” (2012)
  • Author(s): 米倉功治
  • Organizer: 分子システム研究」第1回春合宿
  • Place of Presentation: 浜名湖ロイヤルホテル（静岡）
• [Presentation] イオン共役型エネルギー供与体ExbB/ExbDおよびTonB/ExbB複合体の結晶化構造解析 (2012)
  • Author(s): 渡邊真宏、米倉功治
  • Organizer: 第12回蛋白質科学会
  • Place of Presentation: 名古屋国際会議場（名古屋）
• [Presentation] Purification and crystallization of bacterial ion-coupled energizing protein ExbB (2012)
  • Author(s): M. Watanabe, K. Yonekura
  • Organizer: The 2012 Annual Meeting of American Crystallographic Association
  • Place of Presentation: Boston(MA), USA
• [Presentation] 細菌のイオン共役型エネルギー供与体の構造基盤研究 (2012)
  • Author(s): 渡邊真宏、米倉功治
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡）
• [Presentation] 生体超分子構造の解析 (2011)
  • Author(s): 米倉功治
  • Organizer: 細菌学会中部支部会
  • Place of Presentation: 名古屋大学、名古屋(招待講演)
  • Year and Date: 2011-10-22
• [Presentation] Correlative imaging with CXDI and cryo-EM (2011)
  • Author(s): Koji Yonekura, Saori Maki-Yonekura
  • Organizer: The 4th International Workshop on FEL Science : Science Challenges of XFEL
  • Place of Presentation: Queensland, Australia(招待講演)
  • Year and Date: 2011-08-30
• [Presentation] Structure analysis of biological macromolecules (2011)
  • Author(s): 米倉功治
  • Organizer: The 1st Annual Meeting for Whole-Organism Science Society Joint Meeting with The 10th Annual Meeting of Structural-Biological Whole Cell Project of Thermus thermophilus HB8
  • Place of Presentation: 理研、播磨(招待講演)
  • Year and Date: 2011-08-20
• [Presentation] Cryo-electron microscopy of biological macromolecules (2011)
  • Author(s): Koji Yonekura
  • Organizer: Joint workshop with Wageningen University and SPring-8
  • Place of Presentation: RIKEN, Harima(招待講演)
  • Year and Date: 2011-04-25
• [Presentation] Cryo-electron microscopy of biological macromolecules (2011)
  • Author(s): 米倉功治、眞木さおり
  • Organizer: NHRI workshop
  • Place of Presentation: 新竹、台湾
  • Year and Date: 2011-02-14
• [Presentation] Cryo-electron microscopy of biological macromolecules (2011)
  • Author(s): Koji Yonekura, Saori Maki-Yonekura
  • Organizer: Workshop on structural biology
  • Place of Presentation: Tainan, Taiwan
  • Year and Date: 2011-02-13
• [Presentation] Cryo-electron microscopy of biological macromolecules (2011)
  • Author(s): 米倉功治、眞木さおり
  • Organizer: Workshop on structural biology
  • Place of Presentation: 台南、台湾
  • Year and Date: 2011-02-13
• [Presentation] Cryo-electron microscopy of biological macromolecular structures (2011)
  • Author(s): 米倉功治、眞木さおり
  • Organizer: OIST seminar
  • Place of Presentation: 沖縄 OIST
  • Year and Date: 2011-01-14
• [Presentation] Electron crystallography in biological sciences (2010)
  • Author(s): Koji Yonekura
  • Organizer: The satellite meeting, the 10^(th) conference of the Asian crystallographic association, Daejeon
  • Place of Presentation: Korea
  • Year and Date: 2010-10-29
• [Presentation] Electron crystallography in biological sciences (2010)
  • Author(s): Koji Yonekura
  • Organizer: The satellite meeting, the 10^<th> conference of the Asian crystallographic association
  • Place of Presentation: Daejeon, Korea
  • Year and Date: 2010-10-29
• [Presentation] Electron crystallography in biological sciences (2010)
  • Author(s): 米倉功治
  • Organizer: The satellite meeting, the 10^<th> conference of the Asian crystallographic association
  • Place of Presentation: KBSI, Daejeon, Korea
  • Year and Date: 2010-10-29
• [Presentation] Correlative Imaging---CXDI and Cryo-EM--- (2010)
  • Author(s): 米倉功治、眞木さおり
  • Organizer: The 3rd Workshop on FEL Science : "Emerging X-ray Applications in Biological Systems-II"
  • Place of Presentation: 北海道 北湯沢
  • Year and Date: 2010-10-05
• [Presentation] 低温電子顕微鏡法による生体超分子の構造解析 (2010)
  • Author(s): 米倉功治
  • Organizer: 蛋白質の機能-構造相関解明のための精密構造解析とその方法～水素原子から細胞まで～蛋白研セミナー
  • Place of Presentation: 大阪大学 吹田
  • Year and Date: 2010-10-05
• [Presentation] 低温電子顕微鏡法による生体超分子構造解析 (2010)
  • Author(s): 米倉功治
  • Organizer: ASI-Spring8生物系合同シンポジウム
  • Place of Presentation: 理研 和光市
  • Year and Date: 2010-09-07
• [Presentation] Cryo-electrop microscopy and helical image reconstruction of biological materials (2010)
  • Author(s): 眞木さおり、米倉功治
  • Organizer: アジア若手シンポジウム、日本顕微鏡学会第66回学術講演会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-05-23
• [Presentation] 極低温電子顕微鏡で細菌を『見る』 (2010)
  • Author(s): 米倉功治
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-28
• [Presentation] 極低温電子顕微鏡で細菌を『見る」 (2010)
  • Author(s): 米倉功治
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-28
• [Presentation] 低温電子顕微鏡法による生体分子構造解析の撮像条件について (2010)
  • Author(s): 米倉功治
  • Organizer: 電顕技術開発若手研究部会 第1回ワークショップ
  • Place of Presentation: 熱田、名古屋
  • Year and Date: 2010-01-23
• [Presentation] Cryo-electron microscopy of macromolecular structures (2010)
  • Author(s): 米倉功治・米倉さおり
  • Organizer: 2010 RIKEN Conference
  • Place of Presentation: 理研 播磨
  • Year and Date: 2010-01-13
• [Presentation] 低温電子顕微鏡法の核膜系への応用 (2009)
  • Author(s): 米倉功治・米倉さおり
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] Bioimaging-Cryo-Electron Microscopy (2009)
  • Author(s): 米倉功治
  • Organizer: The Second Workshop on FEL Science : "Emerging X-ray Applications in Biological Systems"
  • Place of Presentation: Kenting, Taiwan
  • Year and Date: 2009-12-07
• [Presentation] 3D Electron Microscopy of Biological Macromolecular Structures (2009)
  • Author(s): 米倉功治
  • Organizer: Workshop on POSTECH-RIKEN Joint Research Program
  • Place of Presentation: 理研 播磨
  • Year and Date: 2009-10-05
• [Presentation] Cryo-electron microscopy of biological macromolecular structures (2009)
  • Author(s): 米倉功治
  • Organizer: SEMINAIRE IBS
  • Place of Presentation: IBS, Grenoble, France
  • Year and Date: 2009-09-07
• [Presentation] High-resolution cryo-electron microscopy of biological macromolecular structures by helical reconstruction (2009)
  • Author(s): Koji Yonekura, Saori Maki-Yonekura
  • Organizer: Microscopy Conference 2009 in Graz
  • Place of Presentation: Graz, Austria
  • Year and Date: 2009-09-02
• [Presentation] 低温電顕微鏡法による生体超分子構造の解析 (2009)
  • Author(s): 米倉功治・米倉さおり
  • Organizer: 高度好熱菌丸ごと一匹プロジェクト 第8回連携研究会
  • Place of Presentation: 理研 播磨
  • Year and Date: 2009-08-23
• [Presentation] 放射光、電子顕微鏡で生命の不思議を"見る" (2009)
  • Author(s): 米倉功治
  • Organizer: 平成21年度「高校生のためのサイエンス・サマーキャンプ」
  • Place of Presentation: 理研 播磨
  • Year and Date: 2009-08-11
• [Presentation] Cryo-electron Microscopy of Biological Macromolecular Structures (2009)
  • Author(s): 米倉功治
  • Organizer: The 1^<st> Workshop on FEL Science "Coherent Intense X-rays in Physics and Biology"
  • Place of Presentation: ロッテホテルチェジュ韓国
  • Year and Date: 2009-02-18
• [Presentation] 電子顕微鏡でモーター分子を見る (2008)
  • Author(s): 米倉功治
  • Organizer: 日本生物物理学会 第46回年会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-04
• [Presentation] 電子顕微鏡でモーター分子を見る (2008)
  • Author(s): 米倉功治
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-04
• [Presentation] 電子顕微鏡でモーター分子を見る (2008)
  • Author(s): 米倉功治
  • Organizer: 日本生物物理学会第46回年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-12-04
• [Presentation] 低温電子顕微鏡法による生体超分子構造の解析 (2008)
  • Author(s): 米倉功治
  • Organizer: 分子研研究会「物質系と生体系での自己組織化-異分野融合的研究の新展開に向けて-」
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2008-08-08
• [Presentation] 低温電子顕微鏡法の生体膜系への応用 (2008)
  • Author(s): 米倉功治
  • Organizer: 平成20年蛋白研セミナー細胞機能制御システムとしての核膜研究〜基礎から臨床応用を目指して〜
  • Place of Presentation: 大阪大学コンベンションセンター
  • Year and Date: 2008-07-24
• [Remarks] 米倉功治、眞木さおり、2009 年(独)電子顕微鏡学会Ernst Ruska 賞受賞。PomAB 単粒子解析の論文(Yonekura et al.,2011, J. Bacteriol.)がハイライトとして紹介されたAmerican Society for Microbiology のMicrobe Magazine の記事へのリンク
  • URL: http://www.microbemagazine.org/index.php?option=com_content&view=article&id=3857:gentlemen-start-your-prokaryotic-motors&catid=876:07-2011-journal-highlights&Itemid=1129
• [Remarks] 米倉生体機構研究室
• [Remarks] 米倉功治、眞木さおり、2009年(独)電子顕微鏡学会Ernst Ruska賞受賞。