| Budget Amount *help |
¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2008: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Research Abstract |
Using oriC plasmid DNA replication in vitro, we analyzed molecular mechanisms recovering the stalled replication fork. We found that a translesion DNA polymerase, Pol IV, readily catalyzed a bypass DNA synthesis at a DNA lesion placed on leading or lagging strand DNA and that the replication fork progression was resumed by this bypass DNA synthesis. We demonstrated that Pol IV rapidly (<15 sec) obstructs the stable interaction between Pol III^* and the beta clamp (the lifetime of the complex >5min), causing the removal of Pol III^* from template DNA. Our study suggests a model in which the interaction between Pol III^* and the beta clamp is mediated by Pol IV to ensure that DNA replication proceeds with minimal interruption.
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