Molecular mechanisms of recovery of stalled DNA replication fork
Project/Area Number |
20370068
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Molecular biology
|
Research Institution | Nara Institute of Science and Technology |
Principal Investigator |
MAKI Hisaji Nara Institute of Science and Technology, バイオサイエンス研究科, 教授 (20199649)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2008: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
|
Keywords | 遺伝学 / 突然変異 / DNA複製 / 遺伝子 / ゲノム |
Research Abstract |
Using oriC plasmid DNA replication in vitro, we analyzed molecular mechanisms recovering the stalled replication fork. We found that a translesion DNA polymerase, Pol IV, readily catalyzed a bypass DNA synthesis at a DNA lesion placed on leading or lagging strand DNA and that the replication fork progression was resumed by this bypass DNA synthesis. We demonstrated that Pol IV rapidly (<15 sec) obstructs the stable interaction between Pol III^* and the beta clamp (the lifetime of the complex >5min), causing the removal of Pol III^* from template DNA. Our study suggests a model in which the interaction between Pol III^* and the beta clamp is mediated by Pol IV to ensure that DNA replication proceeds with minimal interruption.
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Report
(4 results)
Research Products
(62 results)