| Budget Amount *help |
¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2008: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Research Abstract |
We have studied interactions between one of the PCNA loader complexes, human Ctf18-RFC and replicative DNA polymerase, polε, and demonstrated that their binding is mediated by two distinct interactions, one weak and one stable. The common loader structures, including the RFC small subunits (RFC2-5), are responsible for the weak interaction. Three subunits that are specifically required for cohesion, Ctf18, Dcc1, and Ctf8, formed a trimeric complex (18-1-8) and together enabled stable binding with Pol ε. The two interaction modes occurred through the N-terminal half of pol ε, which includes the catalytic domain, and promoted polymorphic modulation of its DNA synthesis.
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