Molecular interactions between Ctf18-RFC loader complex and DNA polymerases
| Project/Area Number |
20370069
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
TSURIMOT Toshiki Kyushu University, 大学院・理学研究院, 教授 (30163885)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥20,280,000 (Direct Cost: ¥15,600,000、Indirect Cost: ¥4,680,000)
Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2008: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | 複製フォーク / 染色体接着 / 修復 / DNAポリメラーゼ / クランプ |
|---|
| Research Abstract |
We have studied interactions between one of the PCNA loader complexes, human Ctf18-RFC and replicative DNA polymerase, polε, and demonstrated that their binding is mediated by two distinct interactions, one weak and one stable. The common loader structures, including the RFC small subunits (RFC2-5), are responsible for the weak interaction. Three subunits that are specifically required for cohesion, Ctf18, Dcc1, and Ctf8, formed a trimeric complex (18-1-8) and together enabled stable binding with Pol ε. The two interaction modes occurred through the N-terminal half of pol ε, which includes the catalytic domain, and promoted polymorphic modulation of its DNA synthesis.
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Report
(4 results)
Research Products
(68 results)
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[Journal Article] Tracking epigenetic histone modifications in single cells using Fab-based live endogenous modification labeling.2011
Author(s)
Hayashi-Takanaka, Y, Yamagata, K, Wakayama, T, Stasevich, T, Kainuma, T, Tsurimoto, T, Tachibana, M, Shinkai, Y, Kurumizaka, H, Nozaki, N, Kimura, H
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Journal Title
Nucleic Acids Research in press
Related Report
Peer Reviewed
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[Journal Article] DNA damage induced ubiquitylation of RFC2 subunit of RFC complex.2008
Author(s)
Tomida J, Masuda Y, Hiroaki H, Ishikawa T, Song I, Tsurimoto T, Tateishi S, Shiomi T, Kamei Y, Kim J, Kamiya K, Vaziri C, Ohmori H, Todo T.
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Journal Title
J Biol Chem. 283
Pages: 9071-9079
Related Report
Peer Reviewed
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[Journal Article] Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks.2008
Author(s)
Tsuji Y, Watanabe K, Araki K, Shinohara M, Yamagata Y, Tsurimoto T, Hanaoka F, Yamamura K, Yamaizumi M, Tateishi, S.
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Journal Title
Genes Cells 13
Pages: 343-354
Related Report
Peer Reviewed
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