Research on transcriptional mediators regulated by signals

• Project/Area Number: 20370074
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: ISHII Shunsuke The Institute of Physical and Chemical Research, 石井分子遺伝学研究室, 主任研究員 (00124785)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000); Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2008: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: コリプレッサー / コアクティベーター / アダプター / Ski / Shn-2 / ヒストンアセチル化 / 複合体 / ヒストンメチル化 / 転写抑制 / Sno

Research Abstract

We found that the Ski corepressor complex contained Smad2/3/4, histone deacetylase HDAC3, and protein arginine N-methyltransferase PRMT5. This suggests that Ski binds to the TGF-β/BMP target genes to keep their low basal transcription levels via HDAC3 and PRMT5. We also showed that Shn-2 adaptor protein has a role to maintain cellular proliferation via suppressing apoptosis during T cell development.

Research Products

• [Journal Article] Dampening of death pathways by Schnurri-2 is essential for T cell development (2011)
  • Author(s): Staton TL, Lazarevic V, Jones DC, Lanser AJ, Takagi T, Ishii S, Glimcher LH
  • Journal Title: Nature 472 Pages: 105-109
  • Peer Reviewed
• [Journal Article] Schnurri-2 deficiency counteracts against bone loss induced by ovariectomy (2011)
  • Author(s): Nagao M, Saita Y, Hanyu R, Hemmi H, Notomi T, Hayata T, Nakamoto T, Kaneko K, Kurosawa H, Ishii S, Ezura Y, Noda M
  • Journal Title: J Cell Physiol. 226 Pages: 573-578
  • Peer Reviewed
• [Journal Article] Conditional knockdown of target gene expression by tetracycline regulated transcription of double strand RNA (2011)
  • Author(s): Hou X, Omi M, Harada H, Ishii S, Takahashi Y, Nakamura H
  • Journal Title: Dev Growth Differ. 53 Pages: 69-75
  • NAID: 10027899337
  • Peer Reviewed
• [Journal Article] Schnurri-2 deficiency counteracts against bone loss induced by ovariectomy. (2011)
  • Author(s): Nagao M, et al.
  • Journal Title: J Cell Physiol Volume: 226 Pages: 573-578
  • Peer Reviewed
• [Journal Article] Conditional knockdown of target gene expression by tetracycline regulated transcription of double strand RNA. (2011)
  • Author(s): Hou, X., et al.
  • Journal Title: Dev Growth Differ. Volume: 53 Pages: 69-75
  • Peer Reviewed
• [Journal Article] Dampening of death pathways by Schnurri-2 is essential for T cell development. (2011)
  • Author(s): Staton, T.L., et al.
  • Journal Title: Nature Volume: (In press)
  • Peer Reviewed
• [Journal Article] Social isolation stress induces ATF-7 phosphorylation and impairs silencing of the 5-HT 5B receptor gene (2010)
  • Author(s): Maekawa T, Kim S, Nakai D, Makino C, Takagi T, Ogura H, Yamada K, Chatton B, Ishii S
  • Journal Title: EMBO J. 29 Pages: 196-208
  • Peer Reviewed
• [Journal Article] The role of ATF-2 family transcription factors in adipocyte differentiation : anti-obesity effects of p38 inhibitors (2010)
  • Author(s): Maekawa T, Jin W, Ishii S
  • Journal Title: Mol.Cell.Biol. 30 Pages: 613-625
  • Peer Reviewed
• [Journal Article] Ribosomal protein L4 positively regulates activity of a c-myb proto-oncogene product (2010)
  • Author(s): Egoh A, Kanesashi S, Kanei-Ishii C, Nomura T, Ishii S
  • Journal Title: Genes Cells 15 Pages: 829-841
  • Peer Reviewed
• [Journal Article] Inhibition of the nuclear import of cubitus interruptus by roadkill in the presence of strong hedgehog signal (2010)
  • Author(s): Seong KH, Akimaru H, Dai P, Nomura T, Okada M, Ishii S
  • Journal Title: PLOS One 5
  • Peer Reviewed
• [Journal Article] Uncoupling of growth plate maturation and bone formation in mice lacking both Schnurri-2 and Schnurri-3 (2010)
  • Author(s): Jones DC, Schweitzer MN, Wein M, Sigrist K, Takagi T, Ishii S, Glimcher LH
  • Journal Title: Proc.Natl.Acad.Sci.USA. 107 Pages: 8254-8258
  • Peer Reviewed
• [Journal Article] Uncoupling of growth plate maturation and bone formation in mice lacking both Schnurri-2 and Schnurri-3. (2010)
  • Author(s): Jones, D.C., et al.
  • Journal Title: Proc.Natl.Acad.Sci.USA. Volume: 107 Pages: 8254-8258
  • Peer Reviewed
• [Journal Article] Inhibition of the nuclear import of cubitus interruptus by roadkill in the presence of strong hedgehog signal. (2010)
  • Author(s): Seong, K.H., et al.
  • Journal Title: PLOS One Volume: 5
  • Peer Reviewed
• [Journal Article] Social isolation stress induces ATF-7 phosphorylation and impairs silencing of the 5-HT 5B receptor gene (2010)
  • Author(s): Maekawa, T., et al.
  • Journal Title: EMBO J. 29 Pages: 196-208
  • Peer Reviewed
• [Journal Article] The role of ATF-2 family transcription factors in adipocyte differentiation : anti-obesity effects of p38 inhibitors (2010)
  • Author(s): Maekawa, T., et al.
  • Journal Title: Mol.Cell.Biol. 30 Pages: 613-625
  • Peer Reviewed
• [Journal Article] Ski co-repressor complexes maintain the basal repressed state of the TGF-beta target gene, SMAD7, via HDAC3 and PRMT5 (2009)
  • Author(s): Tabata T, Kokura K, Ten Dijke P, Ishii S
  • Journal Title: Genes Cells 14 Pages: 17-28
  • Peer Reviewed
• [Journal Article] ATF-2 regulates lipopolysaccharide-induced transcription in macrophage cells (2009)
  • Author(s): Hirose N, Maekawa T, Shinagawa T, Ishii S
  • Journal Title: Biochem.Biophys.Res.Commun. 385 Pages: 72-77
  • Peer Reviewed
• [Journal Article] SKI knockdown inhibits human melanoma tumor growth in vivo (2009)
  • Author(s): Chen D, Lin Q, Box N, Roop D, Ishii S, Matsuzaki K, Fan T, Hornyak TJ, Reed JA, Stavnezer E, Timchenko NA, Medrano EE
  • Journal Title: Pigment Cell Melanoma Res. 22 Pages: 761-772
  • Peer Reviewed
• [Journal Article] Mutations in multiple domains of c-Myb disrupt interaction with CBP/p300 and abrogate myeloid transforming ability (2009)
  • Author(s): Pattabiraman DR, Sun J, Dowhan DH, Ishii S, Gonda TJ
  • Journal Title: Mol Cancer Res. 7 Pages: 1477-1486
  • Peer Reviewed
• [Journal Article] Knock-down of PQBP1 impairs anxiety-related cognition in mouse (2009)
  • Author(s): Ito H, Yoshimura N, Kurosawa M, Ishii S, Nukina N, Okazawa H
  • Journal Title: Hum Mol Genet. 18 Pages: 4239-4254
  • Peer Reviewed
• [Journal Article] Knock-down of PQBP1 impairs anxiety-related cognition in mouse (2009)
  • Author(s): Ito, H., et al.
  • Journal Title: Hum.Mol.Genet. 18 Pages: 4239-4254
  • Peer Reviewed
• [Journal Article] Mutations in multiple domains of c-Myb disrupt interaction with CBP/p300 and abrogate myeloid transforming ability (2009)
  • Author(s): Pattabiraman, D.R., et al.
  • Journal Title: Mol Cancer Res. 7 Pages: 1477-1486
  • Peer Reviewed
• [Journal Article] SKI knockdown inhibits human melanoma tumor growth in vivo (2009)
  • Author(s): Chen, D., et al.
  • Journal Title: Pigment Cell Melanoma Res. 22 Pages: 761-772
  • Peer Reviewed
• [Journal Article] ATF-2 regulates lipopolysaccharide-induced transcription in macrophage cells (2009)
  • Author(s): Hirose, N., et al.
  • Journal Title: Biochem.Biophys.Res.Commun. 385 Pages: 72-77
  • Peer Reviewed
• [Journal Article] Ski co-repressor complexes maintain the basal repressed state of the TGF-beta target gene, SMAD7, via HDAC3 and PRMT5 (2009)
  • Author(s): Tabata T
  • Journal Title: Genes to Cells 14 Pages: 17-28
  • Peer Reviewed
• [Journal Article] A B-Myb complex containing clathrin and filamin is required for mitotic spindle function (2008)
  • Author(s): Yamauchi T, Ishidao T, Nomura T, Shinagawa T, Tanaka Y, Yonemura S, Ishii S
  • Journal Title: EMBO J. 27 Pages: 1852-1862
  • Peer Reviewed
• [Journal Article] ATF-2 controls transcription of Maspin and GADD45 genes independently from p53 to suppress mammary tumors (2008)
  • Author(s): Maekawa T, Sano Y, Shinagawa T, Sakuma T, Nomura S, Licht JD, Ishii S
  • Journal Title: Oncogene 27 Pages: 1045-1054
  • Peer Reviewed
• [Journal Article] Drosophila ATF-2 regulates sleep and locomotor activity in pacemaker neurons (2008)
  • Author(s): Shimizu H, Shimoda M, Yamaguchi T, Seong KH, Ishii S
  • Journal Title: Mol.Cell.Biol. 28 Pages: 6278-6289
  • Peer Reviewed
• [Journal Article] Fbxw7 acts as an E3 ubiquitin ligase that targets c-Myb for nemo-like kinase (NLK)-induced degradation (2008)
  • Author(s): Kanei-Ishii C, Nomura T, Takagi T, Watanabe N, Nakayama KI, Ishii S
  • Journal Title: J.Biol.Chem. 283 Pages: 30540-30548
  • Peer Reviewed
• [Journal Article] Ribosomal stress induces processing of Mybbp1a and its translocation from the nucleolus to the nucleoplasm (2008)
  • Author(s): Yamauchi T, Keough RA, Gonda TJ, Ishii S
  • Journal Title: Genes Cells 13 Pages: 27-39
  • Peer Reviewed
• [Journal Article] Modulation of M2-type pyruvate kinase activity by the cytoplasmic PML tumor suppressor protein (2008)
  • Author(s): Shimada N, Shinagawa T, Ishii S
  • Journal Title: Genes Cells 13 Pages: 245-254
  • Peer Reviewed
• [Journal Article] Increased expression of tyrosine hydroxylase and anomalous neurites in catecholaminergic neurons of ATF-2 null mice (2008)
  • Author(s): Kojima M, Suzuki T, Maekawa T, Ishii S, Sumi-Ichinose C, Nomura T, Ichinose H
  • Journal Title: J. Neurosci.Res. 86 Pages: 544-552
  • Peer Reviewed
• [Journal Article] A B-Myb complex containing clathrin and filamin is required for mitotic spindle function (2008)
  • Author(s): Yamauchi, T., et al.
  • Journal Title: EMBO J. 27 Pages: 1852-1862
  • Peer Reviewed
• [Journal Article] Fbxw7 acts as an E3 ubiquitin ligase that targets c-Myb for nemo-like kinase (NLK)-induced degradation (2008)
  • Author(s): Kanei-Ishii C
  • Journal Title: J. Biol. Chem. 283 Pages: 30540-30548
  • Peer Reviewed
• [Journal Article] ATF-2 controls transcription of Maspin and GADD45α genes independently from p53 to suppress mammary tumors (2008)
  • Author(s): Maekawa T
  • Journal Title: Oncogene 27 Pages: 1045-1054
  • Peer Reviewed
• [Journal Article] Drosophila ATF-2 regulates sleep and locomotor activity in pacemaker neurons (2008)
  • Author(s): Shimizu H
  • Journal Title: Mol. Cell. Biol. 28 Pages: 6278-6289
  • Peer Reviewed
• [Journal Article] Ribosomal stress induces processing of Mybbpla and its translocation from the nucleolus to the nucleoplasm (2008)
  • Author(s): Yamauchi T
  • Journal Title: Genes to Cells 13 Pages: 27-39
  • Peer Reviewed
• [Journal Article] Modulation of M2-type pyruvate kinase activity by the cytoplasmic PML tumor suppressor protin (2008)
  • Author(s): Shimada N
  • Journal Title: Genes to Cells 13 Pages: 245-254
  • Peer Reviewed
• [Presentation] ATF-2ファミリー転写因子を介したストレス依存的なエピジェネティック制御 (2010)
  • Author(s): 石井俊輔、成耆鉉,志水英之,季棟,前川利男
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-14
• [Presentation] ATF-7依存的なヒストンH3メチル化を介したストレスによる転写制御 (2010)
  • Author(s): 前川利男、中井大助、金昇準、吉田圭介、小倉博雄、山田一之、石井俊輔
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-14
• [Presentation] Analysis of relationship between TRIM27 and USP7 through ubiquitiion aeubiqunation (2010)
  • Author(s): 野村照明、Zaman Md Mahabub-Uz、石井俊輔
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-14
• [Presentation] Positive selectionに必要なSchnurr-2のchip on chip法を用いた解析 (2010)
  • Author(s): 高木豪,石井俊輔
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-14
• [Presentation] A possible involvement of Drosophila ATF-2 in epigenetic of Drosophila ATF-2 in epigenetic regulation of innate immune response (2010)
  • Author(s): 中邑亮一,成耆鉉,李棟,石井俊輔
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-14
• [Presentation] Positive selectionに必要な転写因子Schnurr-2のchip on chip法を用いた解析 (2010)
  • Author(s): 高木豪, 石井俊輔
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] ATF-2ファミリー転写因子とエピジェネティック制御 (2010)
  • Author(s): 阪大蛋白研セミナー「ゲノム機能の記憶の成立機序とその制御」
  • Organizer: 石井俊輔
  • Place of Presentation: 東京
  • Year and Date: 2010-11-20
• [Presentation] Inheritance of stress-induced epigenetic change (2010)
  • Author(s): 石井俊輔
  • Organizer: 第15回分生研シンポジウム「エピゲノム研究の最先端」
  • Place of Presentation: 東京
  • Year and Date: 2010-11-12
• [Presentation] Epigenetic regulation by ATF-2 family of transcription factors in response to various stresses Symposium on "Epigenetic inheritance and genome reprogramming" (2010)
  • Author(s): 石井俊輔
  • Organizer: The 62nd Annual Meeting of the Japan Society for Cell Biology
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2010-05-11
• [Presentation] TRIM27タンパク質複合体の精製と解析 (2009)
  • Author(s): 野村照明、石井俊輔
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-15
• [Presentation] ショウジョウバエATF-2はストレス依存的なエピジエネティック変化の遺伝に関与する (2009)
  • Author(s): 成耆鉉、志水英之、野村照明、石井俊輔
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-15
• [Presentation] マウスDP細胞からのSchnurri-2複合体の精製と解析 (2009)
  • Author(s): 高木豪、石井俊輔
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-15
• [Presentation] リボソームタンパク質L4によるc-Myb活性の制御 (2009)
  • Author(s): 江郷彩進之介、石井知恵、石井俊輔
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-15
• [Presentation] ATF-7依存的なヒストンH3メチル化を介したストレスによる転写制御 (2009)
  • Author(s): 川利男,金昇準,小倉博雄,山田一之、中井大助,石井俊輔
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-15
• [Presentation] PQBP1ノックダウンマウスにおける認知記憶機能の低下 (2009)
  • Author(s): 奈津江,黒澤大,石井俊輔,貫名信行,岡澤均
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-15
• [Presentation] Epigenetic regulation by ATF-2 mily of transcription factors in response to various stresses (2009)
  • Author(s): Shunsuke Ishii,Ki-Hyeon Seong, Toshio Maekawa, Hideyuki Shimizu, Seungjoon Kim, Daisuke Nakai
  • Organizer: The 4th International Workshop on Cell Regulation in Division and Arrest
  • Place of Presentation: Okinawa, Japan
  • Year and Date: 2009-12-04
• [Presentation] Role of ATF-2 in epigenetic regulation (2009)
  • Author(s): Ishii, S., et al.
  • Organizer: The 24th Naito Conference on Nuclear Dynamics and RNA[II]
  • Place of Presentation: Sapporo
  • Year and Date: 2009-06-24
• [Presentation] Social isolation stress induces ATF-7 phosphorylation and impairs silencing of the 5-HT 5B receptor gene (2009)
  • Author(s): Toshio Maekawa, Seungjoon Kim, Daisuke Nakai, Chieko Makino, Tsuyoshi Takagi, Hiroo Ogura, Kazuyuki Yamada, Bruno Chatton,Shunsuke Ishii
  • Organizer: The 24th Naito Conference on Nuclear Dynamics and RNA [II]
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2009-06-07
• [Presentation] Drosophila ATF-2 controls the transge stress-induced epigenetic change (2009)
  • Author(s): Ki-Hyeon Seong, Hideyuki Shimizu, Teruaki Nomura,Shunsuke Ishii
  • Organizer: The 24th Naito Conference on Nuclear Dynamics and RNA [II]
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2009-06-07
• [Presentation] Role of ATF-2 in epigenetic regulation (2009)
  • Author(s): Shunsuke Ishii,Ki-Hyeon Seong, Toshio Maekawa, Hideyuki Shimizu, Seungjoon Kim, Daisuke Nakai
  • Organizer: The 24th Naito Conference on Nuclear Dynamics and RNA [II]
  • Place of Presentation: Sapporo, Japan
  • Year and Date: 2009-06-06
• [Presentation] TRIM27結合タンパク質の同定と解析 (2008)
  • Author(s): 野村照明、堂元沙織、高木豪、石井俊輔
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-14
• [Presentation] ショウジョウバエRdxは2つのメカニズムによってHhシグナルを制御する (2008)
  • Author(s): 成耆鉉、野村照明、秋丸裕司、戴平、岡田聖裕、石井俊輔
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-14
• [Presentation] Ski/Sno複合体による転写制御メカニズムの解析 (2008)
  • Author(s): 田畑考統、古倉健嗣、石井俊輔
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-14
• [Presentation] リボソームタンパク質L4によるc-Myb性の制御 (2008)
  • Author(s): 江郷彩子、野村照明、進之介、石井俊輔
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-14
• [Presentation] ATF-7依存的なヒストンH3メチル化を介したストレスによる転写制御 (2008)
  • Author(s): 前川利男,金昇準,小倉博雄,中井大助,石井俊輔
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-14
• [Presentation] Regulation of cell cycle by Myb family transcription factors (2008)
  • Author(s): 石井俊輔
  • Organizer: The 21^<st> Naito Conference on Nuclear Dynamics and RNA [I]
  • Place of Presentation: Yamanashi, Japan
  • Year and Date: 2008-06-20
• [Presentation] A B-Myb complex containing clathrin and filamin is required for mitotic spindle function (2008)
  • Author(s): 石井俊輔
  • Organizer: The 3^<rd> International Workshop on Cell Regulation in Division and Arrest Under Stress
  • Place of Presentation: Okinawa, Japan
  • Year and Date: 2008-04-10
• [Presentation] Zn-finger型転写因子Schnurri-2をむ含複合体の精製と解析 (2008)
  • Author(s): 高木豪、石井俊輔
  • Organizer: 第31回日本分子生物学会年会
  • Place of Presentation: 神戸
• [Remarks] ホームページ
  • URL: http://rtcweb.rtc.riken.jp/lab/mg/mg.html