Small nuclear RNA-mediated mechanism to diversify the genetic information
Project/Area Number |
20370075
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Molecular biology
|
Research Institution | National Institute of Advanced Industrial Science and Technology |
Principal Investigator |
HIROSE Tetsurou National Institute of Advanced Industrial Science and Technology, バイオメディシナル情報研究センター, 研究チーム長 (30273220)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
Fiscal Year 2010: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2009: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2008: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
|
Keywords | 遺伝子発現 / 非コードRNA / RNAプロセシング / RNA修飾 / 細胞核 / RNA / ゲノム / 分子生物学 |
Research Abstract |
To explore the function of orphan snoRNAs that are localized in mammalian cell nucleus, we succeeded in optimization of nuclear RNA knockdown using chemically-modified antisense oligonucleotides. The site-specific RNA methylation activity of o-snoRNP was demonstrated using RNA polymerase I-driven reporter construct. The putative targets of o-snoRNAs were identified from the data of RNA-seq analysis using the RNA samples prepared from o-snoRNA-depleted cells. The novel RNA species were identified from the RNA-seq data of the purified nuclear Cajal body.
|
Report
(4 results)
Research Products
(110 results)
-
-
-
-
-
-
[Journal Article] Thymus-specific noncoding RNA, Thy-ncR1, is a cytoplasmic riboregulator that facilitates hUPF1-dependent degradationof MFAP4 mRNA in specific T-cell leukemia cells.2010
Author(s)
Aoki, K., Harashima, A., Sano, M. Yokoi, T., Nakamura, S., Kibata, M. Hirose T.
-
Journal Title
BMC Mol Biol. 11
Pages: 99-99
Related Report
Peer Reviewed
-
-
-
[Journal Article] Thymus-specific noncoding RNA, Thy-ncR1, is a cytoplasmic riboregulator that facilitates hUPF1-dependent degradation of MFAP4 mRNA in specific T-cell leukemia cells.2010
Author(s)
Aoki, K., Harashima, A., Sano, M., Yokoi, T., Nakamura, S., Kibata, M., Hirose, T.
-
Journal Title
BMC Molecular Biology
Volume: 11
Pages: 99-99
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Transcripts of unknown function in multiple-signaling pathways involved in human stem cell differentiation.2009
Author(s)
Kikuchi, K., Fukuda, M., Ito, T., Inoue, M., Yokoi, T., Chiku, S., Mitsuyama, T., Asai, K., Hirose T., Aizawa, Y.
-
Journal Title
Nucleic Acid Res. 37
Pages: 4987-5000
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
[Journal Article] Transcripts of unknown function in multiple-signaling pathways involved in human stem cell differentiation2009
Author(s)
Kikuchi, K., Fukuda, M., Ito, T., Inoue, M., Yokoi, T., Chiku, S., Mitsuyama, T., Asai, K., Hirose, T., Aizawa, Y.
-
Journal Title
NUCLEIC ACIDS RESEARCH VOL.37
Pages: 4987-5000
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-