Studies on the structure and function of the nucleus in senescent cells

• Project/Area Number: 20370079
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Kyoto University
• Principal Investigator: ISHIKAWA Fuyuki Kyoto University, 大学院・生命科学研究科, 教授 (30184493)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000); Fiscal Year 2009: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2008: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: テロメア / 細胞老化 / NF-kappaB / ヘテロクロマチン / ヒストンH1 / HMGA / lamin / NFkappaB

Research Abstract

We have previously reported that histone H1 disappears and HMGA proteins are increased in cellular senescent cells. In this study, we further examined the structure and function of the nucleus in senescent cells. We found that 1) histone H1 is likely degraded via the ubiqutin-proteasome pathway, 2) the NF-kB pathway is involved in inducing the characteristic gene expression profiles in senescent cells, and 3) the nuclear membrane is structurally and biochemically dysfunctional. These results will provide a means to control the senescence phenotypes.

Research Products

• [Journal Article] Fission yeast Vps1 and Atg8 contribute to oxidative stress resistance (2010)
  • Author(s): Mikawa, T., Kanoh, J., Ishikawa, F.
  • Journal Title: Genes Cells 15 Pages: 229-242
  • Peer Reviewed
• [Journal Article] Fission yeast Vps1 and Atg8 contribute to oxidative stress resistance (2010)
  • Author(s): Mikawa, T.
  • Journal Title: Genes to Cells 15 Pages: 229-242
  • Peer Reviewed
• [Journal Article] RPA-like mammalian Ctc1-Stn1-Ten1 complex binds to single-stranded DNA and protects telomeres independently of the Pot1 pathway (2009)
  • Author(s): Miyake, Y., Nakamura, M., Nabetani, A., Shimamura, S., Tamura, M., Yonehara, S., Saito, M., Ishikawa, F.
  • Journal Title: Mol Cell 36 Pages: 193-206
  • Peer Reviewed
• [Journal Article] A crucial role for adipose tissue p53 in the regulation of insulin resistance (2009)
  • Author(s): Minamino, T., Orimo, M., Shimizu, I., Kunieda, T., Yokoyama, M., Ito, T., Nojima, A., Nabetani, A., Oike, Y., Matsubara, H., Ishikawa, F., Komurao, I.
  • Journal Title: Nat Med 15 Pages: 1082-1087
  • Peer Reviewed
• [Journal Article] Fission yeast Ku protein is required for recovery from DNA replication stress (2009)
  • Author(s): Miyoshi, T., Kanoh, J., Ishikawa, F.
  • Journal Title: Genes Cells 14 Pages: 1091-1103
  • Peer Reviewed
• [Journal Article] Interaction of FLASH with arsenite resistance protein 2 is involved in cell cycle progression at S phase (2009)
  • Author(s): Kiriyama, M., Kobayashi, Y., Saito, M., Ishikawa, F., Yonehara, S.
  • Journal Title: Mol Cell Biol 29 Pages: 4729-4741
  • Peer Reviewed
• [Journal Article] テロメアと幹細胞 (2009)
  • Author(s): 谷村信行、石川冬木
  • Journal Title: 最新医学 64 Pages: 1308-1314
• [Journal Article] 加齢のメカニズム:老化は遺伝子に刷り込まれているのか (2009)
  • Author(s): 石川冬木
  • Journal Title: 画像診断 29 Pages: 120-123
• [Journal Article] テロメアとゲノム安定性 (2009)
  • Author(s): 齊藤基輝、石川冬木
  • Journal Title: 日本臨牀 67 Pages: 107-112
• [Journal Article] Unusual telomeric DNAs in human telomerase-negative immortalized cells (2009)
  • Author(s): Nabetani, A., Ishikawa, F.
  • Journal Title: Mol Cell Biol 29 Pages: 703-713
  • Peer Reviewed
• [Journal Article] RPA-like mammalian Ctc1-Stn1-Ten1 complex binds to single-stranded DNA and protects telomeres independently of the Pot1 pathway (2009)
  • Author(s): Miyake, Y.
  • Journal Title: Molecular Cell 36 Pages: 193-206
  • Peer Reviewed
• [Journal Article] A crucial role for adipose tissue p53 in the regulation of insulin resistance (2009)
  • Author(s): Minamino, T.
  • Journal Title: Nature Medicine 15 Pages: 1082-1087
  • Peer Reviewed
• [Journal Article] Fission yeast Ku protein is required for recovery from DNA replication stress (2009)
  • Author(s): Miyoshi, T.
  • Journal Title: Genes to Cells 14 Pages: 1091-1103
  • Peer Reviewed
• [Journal Article] Interaction of FLASH with arsenite resistance protein 2 is involved in cell cycle progression at S phase (2009)
  • Author(s): Kiriyama, M.
  • Journal Title: Molecular and Cellular Biology 29 Pages: 4729-4741
  • Peer Reviewed
• [Journal Article] テロメアと幹細胞 (2009)
  • Author(s): 谷村信行
  • Journal Title: 最新医学 (6月増刊号) 64 Pages: 1308-1314
  • Peer Reviewed
• [Journal Article] テロメア分子生物学のはじまり (2009)
  • Author(s): 石川冬木
  • Journal Title: 現代化学 465 Pages: 24-28
  • Peer Reviewed
• [Journal Article] Unusual telomeric DNAs in human telomerase-negative immortalized cells. (2009)
  • Author(s): Nabetani, A.
  • Journal Title: Molecular and cellular biology 29 Pages: 703-713
  • Peer Reviewed
• [Journal Article] テロメアとゲノム安定性. (2009)
  • Author(s): 齋藤基輝
  • Journal Title: 日本臨牀(増刊) 67 Pages: 107-112
• [Journal Article] 加齢のメカニズム : 老化は遺伝子に刷り込まれているのか. (2009)
  • Author(s): 石川冬木
  • Journal Title: 画像診断 29 Pages: 120-123
• [Journal Article] Interaction between DNMT1 and DNA replication reactions in the SV40 in vitro replication system (2008)
  • Author(s): Shimamura, S., Ishikawa, F.
  • Journal Title: Cancer Sci 99 Pages: 1960-1966
  • NAID: 10024011906
  • Peer Reviewed
• [Journal Article] ヒトと分裂酵母で保存されたテロメアの最末端構造 (2008)
  • Author(s): 三好知一郎、石川冬木
  • Journal Title: 蛋白質・核酸・酵素 53 Pages: 1850-1857
  • NAID: 40016306371
• [Journal Article] Spatial control of branching within dendritic arbors by dynein-dependent transport of Rab5-endosomes (2008)
  • Author(s): Satoh, D., Sato, D., Tsuyama, T., Saito, M., Ohkura, H., Rolls, M.M., Ishikawa, F., Uemura, T.
  • Journal Title: Nat Cell Biol 10 Pages: 1164-1171
  • Peer Reviewed
• [Journal Article] 分裂酵母Pot1-Tpp1による染色体末端保護とテロメア長制御 (2008)
  • Author(s): 三好知一郎、石川冬木
  • Journal Title: 実験医学 26 Pages: 2601-2605
• [Journal Article] Fission yeast Pot1-Tpp1 protects telomeres and regulates telomere length (2008)
  • Author(s): Miyoshi, T., Kanoh, J., Saito, M., Ishikawa, F.
  • Journal Title: Science 320 Pages: 1341-1344
  • Peer Reviewed
• [Journal Article] TERT promotes cellular and organismal survival independently of telomerase activity. (2008)
  • Author(s): Lee, J.
  • Journal Title: Oncogene 27 Pages: 3754-3760
  • Peer Reviewed
• [Journal Article] Fission yeast Potl-Tppl protects telomeres and regulates telomere length. (2008)
  • Author(s): Miyoshi, T.
  • Journal Title: Science 320 Pages: 1341-1344
  • Peer Reviewed
• [Journal Article] Spatial control of branching within dendritic arbors by dynein-dependent transport of Rab5-endosomes. (2008)
  • Author(s): Sato, D.
  • Journal Title: Nature cell biology 10 Pages: 1164-1171
  • Peer Reviewed
• [Journal Article] Interaction between DNMTl and DNA replication reactions in the SV40 in vitro replication system. (2008)
  • Author(s): Shimamura, S.
  • Journal Title: Cancer science 99 Pages: 1960-1966
  • Peer Reviewed
• [Journal Article] 分裂酵母Potl-Tpplによる染色体末端保護とテロメア長制御. (2008)
  • Author(s): 三好知一郎
  • Journal Title: 実験医学 26 Pages: 2601-2605
• [Journal Article] ヒトと分裂酵母で保存されたテロメアの最末端構造. (2008)
  • Author(s): 三好知一郎
  • Journal Title: 蛋白質・核酸・酵素 53 Pages: 1850-1857
• [Presentation] Telomere metabolism during the cell cycle revealed by analyzing single telomeres in human cells. AACR Special Conference (2010)
  • Author(s): Fuyuki Ishikawa
  • Organizer: he Role of Telomeres an Telomerase in Cancer Research
  • Place of Presentation: The Worthington Renaissance Fort Worth Hotel, Fort Worth, Texas, U.S.A
  • Year and Date: 2010-03-01
• [Presentation] Telomere metabolism during the cell cycle revealed by analyzing single telomeres in human cells (2010)
  • Author(s): 石川冬木
  • Organizer: AACR Special Conference "The Role of Telomeres an Telomerase in Cancer Research"
  • Place of Presentation: The Worthington Renaissance Fort Worth Hotel, Fort Worth, Texas, U.S.A
  • Year and Date: 2010-03-01
• [Presentation] 低容量ストレス反応 (2010)
  • Author(s): 石川冬木
  • Organizer: 島根大学医学部セミナー
  • Place of Presentation: 出雲市、島根大学医学部
  • Year and Date: 2010-01-29
• [Presentation] 分子生物学の過去、現在、未来 (2009)
  • Author(s): 石川冬木
  • Organizer: モノクローナル抗体研究所創立5周年記念講演会
  • Place of Presentation: 横浜市、神奈川歯科大学付属横浜研修センター
  • Year and Date: 2009-12-12
• [Presentation] 細胞老化におけるNF-kB経路の役割. (ポスター発表) (2009)
  • Author(s): 片山雄一郎
  • Organizer: 第32回日本分子物学会年会
  • Place of Presentation: 横浜市、パシフィコ横浜国際会議場
  • Year and Date: 2009-12-11
• [Presentation] A Novel Rpa-Like Mammalian Stc1-Stn1-Ten1 Complex (2009)
  • Author(s): Fuyuki Ishikawa
  • Organizer: 25th Radiation Biology Center International Symposium
  • Place of Presentation: Co-op Inn Kyoto, Kyoto
  • Year and Date: 2009-11-30
• [Presentation] xTRF2 is required for telomere replication in Xenopus egg extracts (2009)
  • Author(s): 石川冬木
  • Organizer: International symposium 'Next-Generation Cancer and Genomic Biology-Telomere, Kinetochore and Genome-'
  • Place of Presentation: Shirankaikan Inamori Hall, Kyoto University, Kyoto
  • Year and Date: 2009-11-02
• [Presentation] Low-Dose Stress Responses (2009)
  • Author(s): Fuyuki Ishikawa
  • Organizer: The 5th International Fission Yeast meeting 'Pombe 2009
  • Place of Presentation: National Olympics Memorial Youth Center, Tokyo
  • Year and Date: 2009-10-29
• [Presentation] Low-Dose Stress Responses (2009)
  • Author(s): 石川冬木
  • Organizer: The 5th International Fission Yeast meeting 'Pombe 2009'
  • Place of Presentation: National Olympics Memorial Youth Center, Tokyo
  • Year and Date: 2009-10-29
• [Presentation] RPA-like Mammalian Ctc1-Stn1-Ten1 Complex Binds to Single-Stranded DNA and Protects Telomeres Independently of the Pot1 Pathway (2009)
  • Author(s): Yasuyuki Miyake, Mirai Nakamura, Akira Nabetani, Shoko Fujita, Shintaro Shimamura, Miki Tamura, Motoki Saito, Fuyuki Ishikawa
  • Organizer: Keystone Symposia on Molecular and Cellular Biology 'Telomere Biology and DNA Repair'
  • Place of Presentation: RACV Royal Pines Resort, Queensland, Australia
  • Year and Date: 2009-10-10
• [Presentation] A novel mammalian RPA-like complex, Ctc1-Stn1-Ten1, protects telomeres (2009)
  • Author(s): Fuyuki Ishikawa
  • Organizer: 68th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Pasifico Yokohama Convention Center, Yokohama
  • Year and Date: 2009-10-02
• [Presentation] A novel RPA-like mammalian complex CST (2009)
  • Author(s): 石川冬木
  • Organizer: French-Japanese Workshop in Life Sciences
  • Place of Presentation: CNRS, Campus Gerard-Megie, Paris, France
  • Year and Date: 2009-09-22
• [Presentation] A novel RPA-like mammalian complex CST (2009)
  • Author(s): 石川冬木
  • Organizer: Japanese-German Cancer Workshop
  • Place of Presentation: Elsa-Braendstroem-Haus, Hamburg, Germany
  • Year and Date: 2009-09-17
• [Presentation] 新規ほ乳類RPA様複合体Ctc1-Stn1-Ten1 (2009)
  • Author(s): 石川冬木
  • Organizer: 第11回生命科学研究科シンポジウム
  • Place of Presentation: 京都市、京都大学芝蘭会館
  • Year and Date: 2009-07-03
• [Presentation] 癌細胞がいかに悪性化するか (2009)
  • Author(s): 石川冬木
  • Organizer: 岡山大学特別講義
  • Place of Presentation: 岡山市、岡山大学
  • Year and Date: 2009-06-03
• [Presentation] Ctc1-Stn1-Ten1 complex (2009)
  • Author(s): Fuyuki Ishikawa
  • Organizer: CSHL meeting 'Telomere and Telomerase'
  • Place of Presentation: Cold Spring Harbor Laboratory, NY, U.S.A
  • Year and Date: 2009-04-29
• [Presentation] 有性生殖の対価としての染色体テロメア (2009)
  • Author(s): 石川冬木
  • Organizer: 長浜バイオ大学バイオセミナー
  • Place of Presentation: 長浜市、長浜バイオ大学
  • Year and Date: 2009-03-17
• [Presentation] 生物学的時間とスケール変換(生物) (2009)
  • Author(s): 石川冬木
  • Organizer: 数理研短期共同研究集会:RIMS共同研究:離散力学系の分子細胞生物学への応用数理
  • Place of Presentation: 京都市、京都大学理学部
  • Year and Date: 2009-01-06
• [Presentation] 生物学的時間とスケール変換(生物) (2009)
  • Author(s): Ishikawa, F.
  • Organizer: 数理研短期共同研究集会 : RIMS共同研究 : 離散力学系の分子細胞生物学への応用数理
  • Place of Presentation: 京都大学理学部
  • Year and Date: 2009-01-06
• [Presentation] Roles of shelterin complex in telomere maintenance (2008)
  • Author(s): Fuyuki Ishikawa
  • Organizer: BMB2008
  • Place of Presentation: Kobe Port Island, Kobe
  • Year and Date: 2008-12-10
• [Presentation] Roles of shelterin complex in telomere maintenance. (2008)
  • Author(s): Ishikawa, F.
  • Organizer: BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2008-12-10
• [Presentation] Sheltrin telomere complex (2008)
  • Author(s): Fuyuki Ishikawa
  • Organizer: 67th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Nagoya Congress Center, Nagoya
  • Year and Date: 2008-10-28
• [Presentation] Sheltrin telomere complex. (2008)
  • Author(s): Ishikawa, F.
  • Organizer: 67th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2008-10-28
• [Presentation] Telomeres and the DNA damage response (2008)
  • Author(s): Fuyuki Ishikawa
  • Organizer: Shelterin complex in fission yeast. EMBO Conference 2008
  • Place of Presentation: Eurotel Victoria Villars, Villars- sur-Ollon, Switzerland
  • Year and Date: 2008-09-18
• [Presentation] Shelterin complex in fission yeast. (2008)
  • Author(s): Ishikawa, F.
  • Organizer: EMBO Conference 2008 "Telomeres and the DNA damageresponse"
  • Place of Presentation: Eurotel Victoria Villars, Villars-sur-Ollon, Switzerland
  • Year and Date: 2008-09-18
• [Presentation] 老化のメカニズム:分子生物学の立場から (2008)
  • Author(s): 石川冬木
  • Organizer: 第27回東京MRI研究会
  • Place of Presentation: 東京都、東京コンファレンスセンター・品川
  • Year and Date: 2008-07-05
• [Presentation] 老化のメカニズム : 分子生物学の立場から. (2008)
  • Author(s): 石川冬木
  • Organizer: 第27回東京MRI研究会
  • Place of Presentation: 東京コンファレンスセンター・品川
  • Year and Date: 2008-07-05
• [Presentation] テロメアの複製機構. (2008)
  • Author(s): 石川冬木
  • Organizer: 第10回生命科学研究科シンポジウム
  • Place of Presentation: 京大会館
  • Year and Date: 2008-06-26
• [Presentation] 低容量ストレス反応 (2008)
  • Author(s): 石川冬木
  • Organizer: 日本生化学会東北支部第74回例会
  • Place of Presentation: 岩手県盛岡市
  • Year and Date: 2008-05-17
• [Presentation] 低容量ストレス反応. (2008)
  • Author(s): 石川冬木
  • Organizer: 日本生化学会東北支部第74回例会
  • Place of Presentation: アイーナいわて県民交流センター
  • Year and Date: 2008-05-17
• [Book] テロメアとテロメラーゼ.がん化学療法・分子標的治療update(西條長宏、西尾和人) (2009)
  • Author(s): 山崎晴丈、石川冬木
  • Publisher: 中外医学社
• [Book] テロメア長制御と末端保護機能:shelterin複合体.がんの分子標的治療(鶴尾隆) (2008)
  • Author(s): 石川冬木
  • Publisher: 南山堂
• [Book] がんの分子標的医療 (2008)
  • Author(s): 鶴尾隆
  • Publisher: 南山堂