Antinomy of cell-cell adhesion and cell proliferation

• Project/Area Number: 20370082
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Aichi Cancer Center Research Institute
• Principal Investigator: INAGAKI Masaki Aichi Cancer Center Research Institute, 発がん制御研究部, 部長 (30183007)
• Co-Investigator(Kenkyū-buntansha): INOKO Akihito 愛知県がんセンター(研究所), 発がん制御研究部, 主任研究員 (30393127) ; KASAHARA Kousuke 愛知県がんセンター(研究所), 発がん制御研究部, 研究員 (90455535) ; IZAWA Ichiro 愛知県がんセンター(研究所), 発がん制御研究部, 室長 (20311441)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥20,800,000 (Direct Cost: ¥16,000,000、Indirect Cost: ¥4,800,000); Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2008: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
• Keywords: 細胞骨格 / 中間径フィラメント / トリコプレイン / 中心小体 / 細胞接着 / 細胞極性

Research Abstract

We studied the functions of Trichoplein and Albatross that bind to keratin intermediate filaments. We found that keratin and Albatross play an important role in the polarization of epithelial cells, and that Trichoplein is localized at centrosomes and is involved in the regulation of primary cilia formation. On the other hand, we found novel regulatory mechanisms of Chk1, which has important functions in the DNA damage checkpoint, via its phosphorylation.

Research Products

• [Journal Article] Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and ninein (2011)
  • Author(s): Ibi, M., Zou, P., Inoko, A., Shiromizu, T., Matsuyama, M., Hayashi, Y., Enomoto, M., Mori, D., Hirotsune, S., Kiyono, T., Tsukita, S., Goto, H. Inagaki, M.
  • Journal Title: J.Cell Sci 124 Pages: 857-864
  • Peer Reviewed
• [Journal Article] Vimentin organization modulates the formation of lamellipodia. (2011)
  • Author(s): Helfand, B.T., Mendez, M.G., Murthy, S.N., Shumaker, D.K., Grin, B., Mahammad, S., Aebi, U., Wedig, T., Wu, Y.I., Hahn, K.M., Inagaki, M., Herrmann, H. Goldman, R.D
  • Journal Title: Mol.Biol. Cell 22 Pages: 1274-1289
  • Peer Reviewed
• [Journal Article] DNA lesions induced by replication stress trigger mitotic aberration and tetraploidy development (2010)
  • Author(s): Ichijima, Y., Yoshioka, K., Yoshioka, Y., Shinohe, K., Fujimori, H., Unno, J., Takagi, M., Goto, H., Inagaki, M., Mizutani, S. Teraoka, H.
  • Journal Title: PLoS ONE 5 Pages: 8821-8821
  • Peer Reviewed
• [Journal Article] Withaferin A targets intermediate filaments glial fibrillary acidic protein and vimentin in a model of retinal gliosis (2010)
  • Author(s): Bargagna-Mohan, P., Paranthan, R.R., Hamza, A., Dimova, N., Trucchi, B., Srinivasan, C., Elliott, G.I., Zhan, C.G., Lau, D.L., Zhu, H., Kasahara, K., Inagaki, M., Cambi, F. Mohan, R
  • Journal Title: J.Biol.Chem 285 Pages: 7657-7669
  • Peer Reviewed
• [Journal Article] Association between vitamin D and calcium intake and breast cancer risk according to menopausal status and receptor status in Japan (2010)
  • Author(s): Kawase, T., Matsuo, K., Suzuki, T., Hirose, K., Hosono, S., Watanabe, M., Inagaki, M., Iwata, H., Tanaka, H. Tajima, K.
  • Journal Title: Cancer Sci 101 Pages: 1234-1240
  • Peer Reviewed
• [Journal Article] 14-3-3γ mediates Cdc25A proteolysis to block premature mitotic entry after DNA damage (2010)
  • Author(s): Kasahara, K., Goto, H., Enomoto, M., Tomono, Y., Kiyono, T. Inagaki, M
  • Journal Title: EMBO J 29 Pages: 2802-2812
  • Peer Reviewed
• [Journal Article] 14-3-3γ mediates Cdc25A proteolysis to block premature mitotic entryafter DNA damage. (2010)
  • Author(s): Kasahara, K., et al.
  • Journal Title: EMBO J Volume: 29 Pages: 2802-2812
  • Peer Reviewed
• [Journal Article] Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and ninein. (2010)
  • Author(s): Ibi, M., et al.
  • Journal Title: J.Cell Sci. Volume: 124 Pages: 857-864
  • Peer Reviewed
• [Journal Article] Withaferin a targets intermediate filaments glial fibrillary acidicprotein and vimentin in a model of retinal gliosis. (2010)
  • Author(s): Bargagna-Mohan P., et al.
  • Journal Title: J.Biol.Chem. 285 Pages: 7657-7669
  • Peer Reviewed
• [Journal Article] DNA lesions induced by replication stress trigger mitotic aberration and tetraploidy development. (2010)
  • Author(s): Ichijima,Y., et al.
  • Journal Title: PLoS ONE 5
  • Peer Reviewed
• [Journal Article] Novel positive feedback loop between Cdk1 and Chk1 in the nucleus during the G2/M transition (2009)
  • Author(s): Enomoto, M., Goto, H., Tomono, Y., Kasahara, K., Tsujimura, K., Kiyono, T. Inagaki, M
  • Journal Title: J. Biol. Chem 284 Pages: 34223-34230
  • Peer Reviewed
• [Journal Article] Novel positive feedback loop between Cdk1 and Chk1 in the nucleus during the G2/M transition. (2009)
  • Author(s): Enomoto, M., et al.
  • Journal Title: J.Biol.Chem. 284 Pages: 34223-34230
  • Peer Reviewed
• [Journal Article] Non-pathogenic protein aggregates in skeletal muscle in MLF1 transgenic mice. (2008)
  • Author(s): Li, Z.F., Wu, X., Jiang, Y., Liu, J., Wu, C., Inagaki, M., Izawa, I., Mizisin, A.P., Engvall, E. Shelton, G.D
  • Journal Title: J. Neurol. Sci 264 Pages: 77-86
  • Peer Reviewed
• [Journal Article] Protein phosphatase 4 catalytic subunit regulates Cdk1 activity and microtubule organization via NDEL1 dephosphorylation. (2008)
  • Author(s): Toyo-oka, K., Mori, D., Yano, Y., Shiota, M., Iwao, H., Goto, H., Inagaki, M., Hiraiwa, N., Muramatsu, M., Wynshaw-Boris, A., Yoshiki, A. Hirotsune, S
  • Journal Title: J.Cell Biol. 180 Pages: 1133-1147
  • Peer Reviewed
• [Journal Article] Palmitoylation of ERBIN is required for its plasma membrane localization. (2008)
  • Author(s): Izawa, I., Nishizawa, M., Hayashi, Y. Inagaki, M
  • Journal Title: Genes Cells 13 Pages: 691-701
  • Peer Reviewed
• [Journal Article] eIF3k regulates apoptosis in epithelial cells by releasing caspase 3 from keratin-containing inclusions (2008)
  • Author(s): Lin, Y.M., Chen, Y.R., Lin, J.R., Wang, W.J., Inoko, A., Inagaki, M., Wu, Y.C. Chen, R.H
  • Journal Title: J.Cell Sci 121 Pages: 2382-2393
  • Peer Reviewed
• [Journal Article] The keratin-binding protein Albatross regulates polarization of epithelial cells (2008)
  • Author(s): Sugimoto, M., Inoko, A., Shiromizu, T., Nakayama, M., Zou, P., Yonemura, S., Hayashi, Y., Izawa, I., Sasoh, M., Uji, Y., Kaibuchi, K., Kiyono, T. Inagaki, M
  • Journal Title: J. Cell Biol 183 Pages: 19-28
  • Peer Reviewed
• [Journal Article] Chk1 phosphorylation at Ser286 and Ser301 occurs with both stalled DNA replication and damage checkpoint stimulation (2008)
  • Author(s): Ikegami, Y., Goto, H., Kiyono, T., Enomoto, M., Kasahara, K., Tomono, Y., Tozawa, K., Morita, A., Kohri, K. Inagaki, M
  • Journal Title: Biochem. Biophys Res.Commun 377 Pages: 1227-1231
  • Peer Reviewed
• [Journal Article] Protein phosphatase 4 catalytic subunit regulates Cdk1 activity and microtubule organization via NDEL1 dephosphorylation (2008)
  • Author(s): Toyo-oka, K., et al.
  • Journal Title: J. Cell Biol. 264 Pages: 77-86
  • Peer Reviewed
• [Journal Article] Palmitoylation of ERBIN is required for its plasma membrane localization (2008)
  • Author(s): Izawa, I., et al.
  • Journal Title: Genes Cells 13 Pages: 691-701
  • Peer Reviewed
• [Journal Article] The keratin-binding protein Albatross regulates polarization of epithelial cells (2008)
  • Author(s): Sugimoto, M., et al.
  • Journal Title: J. Cell Biol. 183 Pages: 19-28
  • Peer Reviewed
• [Journal Article] Nuclear Chkl prevents premature mitotic entry
  • Author(s): Matsuyama, M., Goto, H., Kasahara, K., Kawakami, Y., Nakanishi, M., Kiyono, T., Goshima, N. Inagaki, M
  • Journal Title: J.Cell Sci. (in press)
  • Peer Reviewed
• [Journal Article] Nuclear Chk1 prevents premature mitotic entry.
  • Author(s): Matsuyama, M., et al.
  • Journal Title: J.Cell Sci. Volume: 印刷中
  • Peer Reviewed
• [Presentation] 癌治療に向けての新しい標的分子の探索 (2011)
  • Author(s): 稲垣昌樹
  • Organizer: 第32回泌尿器科分子・細胞研究会
  • Place of Presentation: (津)[シンポジウム]
  • Year and Date: 2011-03-11
• [Presentation] 多彩なリン酸化によってチェックポイントキナーゼ1(Chk1)は機能修飾される. (2010)
  • Author(s): 稲垣昌樹
  • Organizer: 日本分子生物学会第10回春季シンポジウム
  • Place of Presentation: (松島)[シンポジウム]
  • Year and Date: 2010-06-08
• [Presentation] 多彩なリン酸化によってチェックポイントキナーゼ1(Chk1)は機能修飾される (2010)
  • Author(s): 稲垣昌樹
  • Organizer: 日本分子生物学会第10回春季シンポジウム
  • Place of Presentation: ホテル松島大観荘(宮城県)
  • Year and Date: 2010-06-08
• [Presentation] 抗リン酸化ペプチド抗体とチェックポイントシグナル (2010)
  • Author(s): 稲垣昌樹, 笠原広介, 李萍, 後藤英仁
  • Organizer: H22生化学会中部支部例会
  • Place of Presentation: (名古屋)[シンポジウム]
  • Year and Date: 2010-05-29
• [Presentation] 多彩なリン酸化によってチェックポイントキナーゼ1(Chk1)は機能修飾される (2009)
  • Author(s): 笠原広介, 後藤英仁, 稲垣昌樹
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: (横浜)[シンポジウム]
  • Year and Date: 2009-10-02
• [Presentation] Anti-phospho peptide antibodies - a tool to address the regulatory function of intermediate filaments and cell cycle progression in astrocytes (2009)
  • Author(s): 稲垣昌樹
  • Organizer: the 6th European Conference on Intermediate Filaments (Nanofilaments) in Health and Disease
  • Place of Presentation: (Sweden)[シンポジウム]
  • Year and Date: 2009-06-20
• [Presentation] Anti-phospho peptide antibodies -a tool to address the regulatory function of intermediate filaments and cell cycle progression in astrocytes. (2009)
  • Author(s): Masaki Inagaki
  • Organizer: the 6th European Conference on Intermediate Filaments(Nanofilaments)in Health and Disease
  • Place of Presentation: Sweden
  • Year and Date: 2009-06-20
• [Presentation] Signaling and cell architecture (2009)
  • Author(s): 稲垣昌樹
  • Organizer: Receptor Program Ad Hoc Seminar
  • Place of Presentation: (Turku)[シンポジウム]
  • Year and Date: 2009-06-15
• [Presentation] Cell cycle and architecture (2009)
  • Author(s): 稲垣昌樹
  • Organizer: Regulation and Manipulation of Information Flow within Dynamic Protein and Lipid Environments (SFB 645 Workshop Cell Architecture and Cell Signalling)
  • Place of Presentation: (Bonn)[シンポジウム]
  • Year and Date: 2009-06-12
• [Presentation] Trichoplein, a keratin-binding protein, maker of microtubule-organization and breaker of cilia assembly (2009)
  • Author(s): Inoko, A., Inagaki M.
  • Organizer: Cell Cycle and Cell Architecture
  • Place of Presentation: (名古屋)[シンポジウム]
  • Year and Date: 2009-02-27
• [Presentation] Trichoplein regulates microtubule anchoring and suppresses a cilia assembly program at the mother centriole (2009)
  • Author(s): 稲垣昌樹
  • Organizer: GCOE第一回国際シンポジウム
  • Place of Presentation: (名古屋)[シンポジウム]
  • Year and Date: 2009-01-23
• [Presentation] GCOEと愛知県がんセンター (2008)
  • Author(s): 稲垣昌樹
  • Organizer: GCOE国内シンポジウム
  • Place of Presentation: (名古屋)[シンンポジウム]
  • Year and Date: 2008-11-14
• [Presentation] Chk1 phosphorylation by Cyclin-dependent kinases (Cdks) (2008)
  • Author(s): Goto, H., Inagaki M.
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: (名古屋)[シンポジウム]
  • Year and Date: 2008-10-28
• [Presentation] Novel TPHD proteins : keratin-binding proteins act as the regulator for the cell-cell adhesion (2008)
  • Author(s): Inoko, A., Inagaki M.
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: (名古屋)[シンポジウム]
  • Year and Date: 2008-10-28
• [Presentation] Novel TPHD proteins : keratin-binding proteins act as the regulator for the cell-cell adhesion (2008)
  • Author(s): Inoko, A., Inagaki, M.
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2008-10-28
• [Book] (編)編細胞周期フロンティア (2010)
  • Author(s): 佐方功幸、稲垣昌樹、岸本健雄
  • Total Pages: 268
  • Publisher: 共立出版
• [Book] 「G2/M移行期におけるCDK1の活性化およびチェックポイント解除機構」細胞周期フロンティア (2010)
  • Author(s): 後藤英仁、稲垣昌樹
  • Publisher: 共立出版
• [Book] 「抗リン酸化ペプチド抗体と細胞周期研究細胞周期フロンティア (2010)
  • Author(s): 笠原広介、稲垣昌樹
  • Publisher: 共立出版
• [Book] 「細胞周期研究の現状と展望」細胞周期フロンティア (2010)
  • Author(s): 稲垣昌樹、佐方功幸
  • Publisher: 共立出版
• [Book] 細胞周期フロンティア (2010)
  • Author(s): 佐方功幸, ら 編
  • Total Pages: 268
  • Publisher: 共立出版株式会社
• [Book] リン酸化抗体と細胞周期細胞工学 (2009)
  • Author(s): 大室(松山)有紀、稲垣昌樹
  • Publisher: 秀潤社
• [Book] Close UP実験法特定部位の翻訳後修飾を特異的に認識する抗体作製法.抗リン酸化(ペプチド)抗体作製法,実験医学 (2008)
  • Author(s): 後藤英仁、稲垣昌樹
  • Publisher: 羊土社
• [Book] 「中間径フィラメント」生化学事典
  • Author(s): 衣斐美歩、稲垣昌樹
  • Publisher: 朝倉書店(in press)