The calcium signaling pathway that initiates embryonic development in fertilize amphibian eggs.
| Project/Area Number |
20370085
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Nagoya University (2009-2010)
Tokyo Institute of Technology (2008) |
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Principal Investigator |
OHSUMI Keita 名古屋大学, 理学研究科, 教授 (20221822)
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| Co-Investigator(Kenkyū-buntansha) |
IWABUCHI Mari 名古屋大学, 大学院・理学研究科, 講師 (40275350)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2009: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2008: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Keywords | 胚発生 / 受精 / カルシウムシグナル / カエル卵 |
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| Research Abstract |
At fertilization, a transient increase of cytoplasmic calcium ions(Ca^<2+>) triggers various activation responses in animal eggs. In amphibian, these responses include exit from meiotic arrest and cortical remodeling observed as activation contraction. We have investigated substrates for calcineurin in inducing the meiotic exit and regulatory mechanisms for the activation contraction, using cell-free extracts and cell cortices isolated from Xenopus eggs. The results indicated that Erp1 and Cdc20, both of which are essential for cyclin B destruction which leads to the meiotic exit, are dephosphorylated upon activation. The phosphatase(s) responsible for their dephosphorylation remains to be identified. We have also established the method to isolate the cell cortex from oocytes and eggs and to elute their cortical proteins. Using these materials, we found that anillin, an actin cytoskeletal regulator protein, increases during oocytes maturation and is dephosphorylated upon activation. Thus, the cortical isolation method developed in this study has enabled the biochemical analyses of cell cortex.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Kendrin is a novel substrate for separase involved in the licensing of centriole duplication2012
Author(s)
Matsuo, K., Ohsumi, K., Iwabuchi, M., Kawamata, T., Ono, Y., Takahashi, M
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Journal Title
Curr. Biol.
Volume: 22
Pages: 915-921
Related Report
Peer Reviewed
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[Journal Article] Oocyte-type linker histone B4 is required for transdifferentiation of somatic cells in vivo.2010
Author(s)
Maki, N., Suetsugu-Maki, R., Sano, S., Nakamura, K., Nihimura, O., Tarui, H., Del Rio-Tsonis, K., Ohsumi, K., Agata, K., Tsonis, P. A
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Journal Title
FASEBJ.
Volume: 24
Pages: 3462-3467
Related Report
Peer Reviewed
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