Role of ubiquitin system in Xenopus gastrulation movements

• Project/Area Number: 20370090
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: KINOSHITA Noriyuki 基礎生物学研究所, 形態形成研究部門, 准教授 (30300940)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000); Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2009: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2008: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
• Keywords: 原腸形成 / 細胞接着 / 細胞運動 / ユビキチン化 / Wntシグナル

Research Abstract

Paraxial protocadherin(PAPC) has been shown to be involved in gastrulation cell movements during early embryogenesis. It is first expressed in the dorsal marginal zone at early gastrula stage and subsequently restricted to paraxial mesoderm during gastrulation in Xenopus and zebrafish. Using Xenopus embryos, we found that PAPC is regulated also at the protein level is degraded and excluded from the plasma membrane in the axial mesoderm at late gastrula stage. Our findings suggest a novel mechanism of regulation of a cell adhesion protein and that this system plays a crucial role in vertebrate embryogenesis.

Research Products

• [Journal Article] Wnt/PCPシグナルにおけるユビキチンシステムの役割 (2010)
  • Author(s): 木下典行
  • Journal Title: 医学のあゆみ Volume: 233 Pages: 978-982
• [Journal Article] 脊椎動物のPCPシグナル (2008)
  • Author(s): 木下典行
  • Journal Title: 実験医学 Volume: 26 Pages: 374-379
• [Presentation] Prickleとβ-TrCPはツメガエル初期発生においてparaxial protocadherinをユビキチン化を介して調節することにより細胞接着を制御する (2011)
  • Author(s): 甲斐理武,木下典行
  • Organizer: 日本発生生物学会
  • Place of Presentation: 沖縄コンベンションセンター
• [Presentation] Dissecting biological functions of a Wnt/PCP pathway component Prickle (2010)
  • Author(s): Masatake kai, Noriyuki Kinoshita
  • Organizer: The 16^<th> International Conference of the INTERNATIONAL SOCIETY OF DIFFERENTIATION
  • Place of Presentation: 奈良県立公会堂
• [Presentation] Ubiquitin system regulates the localization of Wnt/PCP signal proteins in Xenopus embryonic cells (2010)
  • Author(s): Noriyuki Kinoshita, Masatake kai
  • Organizer: The 16^<th> International Conference of the INTERNATIONAL SOCIETY OF DIFFERENTIATION
  • Place of Presentation: 奈良県立公会堂
• [Presentation] アフリカツメガエル胚細胞においてユビキチンシステムがPCPタンパク質の局在を制御する (2010)
  • Author(s): 木下典行
  • Organizer: 日本発生生物学会
  • Place of Presentation: 京都国際会議場
• [Presentation] The ubiquitin system regulates PCP protein localizations in Xenopus embryonic cells. (2010)
  • Author(s): Noriyuki Kinoshita
  • Organizer: Annual meeting of the Japanaese Society of Developmental Biolodists
  • Place of Presentation: 国立京都国際会館(京都)
• [Presentation] The ubiquitin system regulates PCP protein localizations in Xenopus embryonic cells. (2010)
  • Author(s): Noriyuki Kinoshita
  • Organizer: The international conference of the Internatitonal Society of Differentiation
  • Place of Presentation: 奈良県新公会堂(奈良)
• [Presentation] Wnt/PCPシグナルのユビキチン化酵素による制御 (2009)
  • Author(s): 木下典行
  • Organizer: 九州大学GCOEシンポジウム
  • Place of Presentation: 九州大学医学部
  • Year and Date: 2009-03-24
• [Presentation] Wnt signalling regulates paxillin ubiquitination essential for mesodermal cell motility (2008)
  • Author(s): Noriyuki Kinoshita
  • Organizer: Santa Cruz Developmental Biology Meeting at University of California, Santa Cruz
  • Place of Presentation: アメリカ合衆国