| Budget Amount *help |
¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2008: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
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| Research Abstract |
For elucidation of the mechanism of evolution, we have developed thermo-tolerant E. coli and analyzed mutation of genome DNA, gene expression (transcriptome), and protein expression and thermo-stability (proteome) of several strains of E. coli through thermo-tolerant evolution process. Whole-genome sequence analysis revealed the transition from positive to neutral in mutation fixation, accompanied with a considerable escalation of spontaneous substitution rate in the late fitness recovery phase. Moreover, transcriptome analysis revealed transition from increase to convergence in fluctuation of gene expression, and proteome analysis revealed that no remarkable change in thermo-stability of expressed proteins.
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