| Budget Amount *help |
¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2008: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Research Abstract |
Ancestral enzymes of translation system (GlyRS), metabolic system (IPMDH and NDK) and replication system (gyrase) were resurrected, their genes were constructed and expressed in E. coli. The purified enzymes of translation and replication systems showed higher thermal stability than counter parts of an extreme thermophile. Several mutants of previously constructed ancestral NDH were analyzed. The results showed that ancestral enzymes of Bacteria, Archaea and Commonote are extremely (>100℃) thermostable.
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