Study on the common ancestors of Archaea, Bacteria and Commonote based on the resurrected enzymes.
| Project/Area Number |
20370094
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
YAMAGISHI Akihiko Tokyo University of Pharmacy and Life Science, 生命科学部, 教授 (50158086)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOBORI Shin-Ichi 東京薬科大学, 生命科学部, 講師 (40291702)
AKANUMA Satoshi 東京薬科大学, 生命科学部, 助教 (10321720)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2008: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Keywords | 機能進化 / 祖先型 / 古細菌 / 真正細菌 / NDK / ジャイレース / 耐熱性 / 活性 / コンセンサス / 祖先型酵素 / 全生物の共通祖先 / IPMDH / グリシルtRNA合成酵素 / 超好熱菌 |
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| Research Abstract |
Ancestral enzymes of translation system (GlyRS), metabolic system (IPMDH and NDK) and replication system (gyrase) were resurrected, their genes were constructed and expressed in E. coli. The purified enzymes of translation and replication systems showed higher thermal stability than counter parts of an extreme thermophile. Several mutants of previously constructed ancestral NDH were analyzed. The results showed that ancestral enzymes of Bacteria, Archaea and Commonote are extremely (>100℃) thermostable.
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Report
(4 results)
Research Products
(76 results)
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[Journal Article] Solvent site-dipole field accompanying protein-ligand approach process.2008
Author(s)
Takano, N., Umezawa., J., Ikebe, K., Sonobe, Y., Yagisawa, R., Ito, J., Hamasaki, N., Mitomo, D., Miyagawa, H., Yamagihshi, A., Higo, J.
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Journal Title
Chem-Bio. Informatics J. 8
Pages: 14-24
Related Report
Peer Reviewed
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