Analysis of molecular mechanism, origin and diversity of gametophytic self-incompatibility systems

• Project/Area Number: 20380003
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Chiba University
• Principal Investigator: SASSA Hidenori 千葉大学, 大学院・園芸学研究科, 准教授 (50295507)
• Co-Investigator(Kenkyū-buntansha): USHIJIMA Koichiro 岡山大学, 大学院・自然科学研究科, 助教 (20379720) ; 古藤田 信博 (独)農業・食品産業技術研究機構, 果樹研究所, 主任研究員 (50355426)
• Co-Investigator(Renkei-kenkyūsha): KOTODA Nobuhiro (独)農業・食品産業技術研究機構, 果樹研究所, 主任研究員 (50355426)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2009: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2008: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: 遺伝子 / タンパク質 / 自家不和合性 / 雌ずい / 花粉 / RNase / バラ科 / ナス科 / Rnase

Research Abstract

Analysis of candidates for pollen-determinant in Japanese pear and apple of Rosaceae suggested that multiple and related F-box genes, SFBB, are collaboratively detoxify non-self pistil determinant, S-RNase. Isolation and functional analysis of HT-B gene, a non-S-specific self-incompatibility factor, of petunia showed that, unlike other solanaceous species, even a dramatic suppression of HT-B transcript resulted in partial breakdown of self-incompatibility in petunia. This may suggest slight functional divergence of HT-B gene in different species of Solanaceae.

Research Products

• [Journal Article] Sequence divergence and loss-of-function phenotypes of S locus F-box brothers (SFBB) genes are consistent with non-self recognition by multiple pollen determinants in self-incompatibility of Japanese pear (Pyrus pyrifolia) (2011)
  • Author(s): Kakui, H., Kato, M., Ushijima, K., Kitaguchi, M., Kato, S. Sassa, H.
  • Journal Title: Plant J. Volume: 68 Pages: 1028-1038
  • Peer Reviewed
• [Journal Article] Sequence divergence and loss-of-function phenotypes of S locus F-box brothers genes are consistent with non-self recognition by multiple pollen determinants in self-incompatibility of Japanese pear (Pyrus pyrifolia) (2011)
  • Author(s): Hiroyuki Kakui, Masaki Kato, Koichiro Ushijima, Miyoko Kitaguchi, Shu Kato, Hidenori Sassa
  • Journal Title: The Plant Journal Volume: 68 Issue: 6 Pages: 1028-1038
  • DOI: 10.1111/j.1365-313x.2011.04752.x
  • Peer Reviewed
• [Journal Article] Apple S locus region represents a large cluster of related, polymorphic and pollen-specific F-box genes (2010)
  • Author(s): Minamikawa, M., Kakui, H., Wang, S., Kotoda, N., Kikuchi, S., Koba, T. Sassa, H.
  • Journal Title: Plant Mol.Biol. Volume: 74 Pages: 143-154
  • Peer Reviewed
• [Journal Article] Apple S locus region represents a large cluster of related, polymorphic and pollen-specific F-box genes. (2010)
  • Author(s): Minamikawa, M., Kakui, H., Wang, S., Kotoda, N., Kikuchi, S., Koba, T., Sassa, H.
  • Journal Title: Plant Mol Biol 74 Pages: 143-154
  • Peer Reviewed
• [Journal Article] Pollen-expressed F-box gene family and mechanism of S-RNase-based gametophytic self-incompatibility (GSI) in Rosaceae. (2010)
  • Author(s): Sassa, H., Kakui, H., Minamikawa, M
  • Journal Title: Sex.Plant Reprod. 23 Pages: 39-43
  • Peer Reviewed
• [Journal Article] Apple S locus region represents a large cluster of related, polymorphic and pollen-specific F-boxgenes (2010)
  • Author(s): Minamikawa, M, Kakui, H., Wang, S., etal.
  • Journal Title: Plant Molecular Biology Volume: 74 Pages: 143-154
  • Peer Reviewed
• [Journal Article] Pollen-expressed F-box gene family and mechanism of S-RNase-based gametophytic self-incompatibility(GSI)in Rosaceae (2010)
  • Author(s): Sassa, H., Kakui, H., Minamikawa, M
  • Journal Title: Sex.Plant Reprod. 23 Pages: 39-43
  • Peer Reviewed
• [Journal Article] Identification and functional analysis of pistil-partself-incompatibility factor HT-B ofPetunia (2009)
  • Author(s): Puerta, A. R., Ushijima, K., Koba, T. Sassa, H.
  • Journal Title: J. Exp. Bot. Volume: 60 Pages: 1309-1318
  • Peer Reviewed
• [Journal Article] Pistil non-S-specificself-incompatibility factor HT-B is tightly linked to the HT-like gene HTL in Petunia (2009)
  • Author(s): Puerta, A. R., Takagawa, M. Sassa, H.
  • Journal Title: Breed. Sci Volume: 59 Pages: 99-101
  • Peer Reviewed
• [Journal Article] Determination of partial genomic sequences and development of a CAPS system of the S-RNase gene for the identification of 22 Shaplotypes of apple (2009)
  • Author(s): Kim, H., Kakui, H., Kotoda, N., Hirata, Y., Koba, T. Sassa, H.
  • Journal Title: Mol. Breed Volume: 23 Pages: 463-472
  • Peer Reviewed
• [Journal Article] Identification and functional analysis of pistil-part self-incompatibility factor HT-B of Petunia. (2009)
  • Author(s): Puerta, A.R., Ushijima, K., Koba, T., Sassa, H.
  • Journal Title: J Exp Bot 60 Pages: 1309-1318
  • Peer Reviewed
• [Journal Article] Pistil non-S-specific self-incompatibility factor HT-B is tightly linked to the HT-like gene HTL in Petunia. (2009)
  • Author(s): Puerta, A.R., Takagawa, M., Sassa, H.
  • Journal Title: Breed Sci 59 Pages: 99-101
  • NAID: 110007122744
  • Peer Reviewed
• [Journal Article] Determination of partial genomic sequences and development of a CAPS system of the S-RNase gene for the identification of 22 S haplotypes of apple (Malus × domestica Borkh.). (2009)
  • Author(s): Kim, H., Kakui, H., Kotoda, N., Hirata, Y., Koba, T., Sassa, H.
  • Journal Title: Mol Breed 23 Pages: 463-472
  • Peer Reviewed
• [Journal Article] Identification and functional analysis of pistil-part self-incompatibility factor HT-B of Petunia. (2009)
  • Author(s): Puerta, A. R. , Ushij ima, K. , Koba, T. and *Sassa, H.
  • Journal Title: J Exp Bot 60 Pages: 1309-1318
  • Peer Reviewed
• [Journal Article] Pistil non-S-specific self-incompatibility factor HT-18 is tightly linked to the HT-like gene HTL in Petunia. (2009)
  • Author(s): Puerta, A. R., Takagawa, M. and *Sassa, H.
  • Journal Title: Breed Sci 59 Pages: 99-101
  • Peer Reviewed
• [Journal Article] Determination of partial genomic sequences and development of a CAPS -system of the S-RNase gene for the identification of 22 S haplotypes of apple (Malus x domestica Borkh.). (2009)
  • Author(s): Kim, H. , Kakui, H., Kotoda, Na , Hirata, Y., Koba, T. and *Sassa, H.
  • Journal Title: Mol Breed 23 Pages: 463-472
  • Peer Reviewed
• [Journal Article] Identification of self-incompatibility related proteins in the pistil of Japanes epear (Pyrus pyrifolia (Burm. f.)) by proteome analysis
  • Author(s): Uchida, A., Sassa, H., Takenaka, S., Sakakibara, Y., Suiko, M. Kunitake, H.
  • Journal Title: Plant Omics Volume: (in press)
  • Peer Reviewed
• [Presentation] Non-self recognition by multiple F-box genes in self-incompatibility of Japanese pear (Pyrus pyrifolia) (2012)
  • Author(s): Sassa, H., Kakui, H., Kato, M., Ushijima, K.
  • Organizer: Plant and Animal Genome XX
  • Place of Presentation: San Diego, CA, USA(招待講演)
  • Year and Date: 2012-01-14
• [Presentation] Non-self recognition by multiple F-box genes inself-incompatibility of Japanese pear (Pyrus pyrifolia) (2012)
  • Author(s): Sassa, H., Kakui, H., Kato, M. Ushijima, K.
  • Organizer: Plant and AnimalGenome XX
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Structural features of the S locus of apple (2011)
  • Author(s): Sassa H., Wang, S., Minamikawa, M., Kakui, H., Kikuchi, S., Koba, T.
  • Organizer: Floral Biology and S-incompatibility in fruit species
  • Place of Presentation: San Michele all'Adige, Italy(招待講演)
  • Year and Date: 2011-06-23
• [Presentation] ニホンナシ品種「なつひかり」の弱い自家不和合性の解析 (2011)
  • Author(s): 加藤雅樹, 加藤修, 北口美代子, 佐々英徳
  • Organizer: 日本育種学会第120回講演会
  • Place of Presentation: 福井県立大学
• [Presentation] Structural features of the S locus of apple (2011)
  • Author(s): Sassa H., Wang, S., Minamikawa, M., Kakui, H., Kikuchi, S. Koba, T.
  • Organizer: Floral Biology and S-incompatibility in fruit species
  • Place of Presentation: San Michele all'Adige, Italy
• [Presentation] リンゴにおける自家不和合性関連遺伝子SBP1(S-RNase binding protein1)ホモログの同定と解析 (2010)
  • Author(s): 藤井大輔、角井宏行、古藤田信博、牛島幸一郎、佐々英徳
  • Organizer: 日本育種学会
  • Place of Presentation: 秋田県立大学
  • Year and Date: 2010-09-25
• [Presentation] ニホンナシにおける雌ずい側自家和合性変異ハプロタイプS4smの花粉側特異性の解析 (2010)
  • Author(s): 角井宏行、加藤雅樹、加藤修、北口美代子、佐々英徳
  • Organizer: 日本育種学会
  • Place of Presentation: 秋田県立大学
  • Year and Date: 2010-09-25
• [Presentation] Characterization of the F-boxgene cluster linked to the S locus of apple (2010)
  • Author(s): Minamikawa, M, Kakui, H., Wang, S., etal.
  • Organizer: XXI International Congress on Sexual Plant Reproduction
  • Place of Presentation: ブリストル大学
  • Year and Date: 2010-08-03
• [Presentation] S-RNase and SFBB family F-box genes are located in sub-telomeric and Heterochromatic chromosomal regionin apple (2010)
  • Author(s): Wang, S., Kakui, H., Kikuchi, S.Koba, T., Sassa, H.
  • Organizer: XXI International Congress on Sexual Plant Reproduction
  • Place of Presentation: ブリストル大学
  • Year and Date: 2010-08-03
• [Presentation] S-RNase and SFBB family F-box genes are located in sub-teromeric and heterochromatic chromosomal region in apple (2010)
  • Author(s): Wang, S., Kakui, H., Kikuchi, S., Koba,T. Sassa H.
  • Organizer: XXI International Congress on Sexual Plant Reproduction
  • Place of Presentation: Bristol, UK
• [Presentation] Characterization of the F-box gene cluster linked to the S locus of apple (2010)
  • Author(s): Minamikawa, M., Kakui, H., Wang, S., Kotoda, N., Kikuchi, S., Koba, T. Sassa, H.
  • Organizer: XXI International Congress on Sexual Plant Reproduction
  • Place of Presentation: Bristol, UK
• [Presentation] Analysis of pollen-part S specificity of S4sm, a stylar-part self-compatible S haplotype in Japanese pear (2010)
  • Author(s): Kakui, H., Sassa H., Kitaguchi, M., Kato, S. Kato , M.
  • Organizer: XXI International Congress on Sexual Plant Reproduction
  • Place of Presentation: Bristol, UK
• [Presentation] リンゴにおける自家不和合性関連遺伝子SBP1 (S-RNase binding protein1)ホモログの同定と解析 (2010)
  • Author(s): 藤井大輔, 角井宏行, 古藤田信博, 牛島幸一郎, 佐々英徳
  • Organizer: 日本育種学会第118回講演会
  • Place of Presentation: 秋田県立大学
• [Presentation] ニホンナシにおける雌ずい側自家和合性変異ハプロタイプS4smの花粉側特異性の解析 (2010)
  • Author(s): 角井宏行, 加藤雅樹, 加藤修, 北口美代子, 佐々英徳
  • Organizer: 日本育種学会第118回講演会
  • Place of Presentation: 秋田県立大学
• [Presentation] S-RNase and SFBB family F-box genes are located in sub-teromeric and heterochromatic chromosomal region in apple. (2010)
  • Author(s): Wang, S., Kakui, H., Kikuchi, S., Koba, T., Sassa H.
  • Organizer: XXI International Congress on Sexual Plant Reproduction.PSR6.22, Abstract
  • Place of Presentation: Bristol, UK
• [Presentation] Characterization of the F-box gene cluster linked to the S locus of apple. (2010)
  • Author(s): Minamikawa, M., Kakui, H., Wang, S., Kotoda, N., Kikuchi, S., Koba, T., Sassa, H.
  • Organizer: XXI International Congress on Sexual Plant Reproduction PSR6.23, Abstract
  • Place of Presentation: Bristol, UK
• [Presentation] Analysis of pollen-part S specificity of S4sm, a stylar-part self-compatible S haplotype in Japanese pear. (2010)
  • Author(s): Kakui, H., Sassa H., Kitaguchi, M., Kato, S., Kato , M.
  • Organizer: XXI International Congress on Sexual Plant Reproduction PSR6.25, Abstract
  • Place of Presentation: Bristol, UK
• [Presentation] リンゴS遺伝子座に連鎖するF-box遺伝子群 (2009)
  • Author(s): 南川舞、角井宏行、古藤田信博、佐々英徳
  • Organizer: 日本育種学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-09-26
• [Presentation] Identification and functional analysis of non-S-specific pistil-part self-incompatibility factor HT-B of Petunia (2009)
  • Author(s): Puerta AR, Ushijima K, Koba T, Sassa H.
  • Organizer: 日本育種学会第115回講演会
  • Place of Presentation: つくば国際会議場
• [Presentation] リンゴS遺伝子座に連鎖するF-box遺伝子群の解析 (2009)
  • Author(s): 南川舞, 角井宏行, 古藤田信博, 佐々英徳
  • Organizer: 日本育種学会第116回講演会
  • Place of Presentation: 北海道大学
• [Presentation] S-RNase遺伝子のCAPS分析によるリンゴ品種のS対立遺伝子22種類の識別 (2008)
  • Author(s): Kim HT, 角井宏行, 古藤田信博, 木庭卓人, 佐々英徳
  • Organizer: 日本育種学会第114回講演会
  • Place of Presentation: 滋賀県立大学
• [Presentation] Characterization of multiple F-box genes linked to the S locus of apple and Japanese pear (2008)
  • Author(s): Sassa, H., Kakui, H., Minamikawa, M.
  • Organizer: Frontiers of Sexual Plant Reproduction III
  • Place of Presentation: Tucson, USA
• [Presentation] Characterization of multiple F-box genes linked to the S locus of apple and Japanese pear. (2008)
  • Author(s): Sassa, H., Kakui, H., Minamikawa, M.
  • Organizer: Frontiers of Sexual Plant Reproduction III, Abstract
  • Place of Presentation: Tucson, USA(招待講演)