Breeding basis for stress resistant plants by molecular signal analysis

• Project/Area Number: 20380004
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Saitama University (2009-2010); The University of Tokyo (2008)
• Principal Investigator: UCHIMIYA Hirofumi 埼玉大学, 環境科学研究センター, 教授 (50142229)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2009: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2008: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: ストレス応答 / シグナル因子 / NAD / 補酵素 / 代謝物質 / 遺伝子 / 酸化還元反応 / 分子育種 / 代謝物 / シロイヌナズナ / イネ / NADK / CE-MS / 代謝解析 / 光合成

Research Abstract

In plants, stress responses is a mechanism underlying cell defense functions to oxidative damage. In this regard, NAD coenzymes are typical regulators. Here, we focused on such enzymes (genes) to clarify environmental responses of plants against external stresses. At end, we were able to establish new strategies concerning molecular improvements of plants against environmental constrains.

Research Products

• [Journal Article] Fate of 13C in Metabolic Pathways and Effects of High CO2 on the Alteration of Metabolites in Rumex obtusifolius L. (2011)
  • Author(s): Miyagi, A., 0Takahara, K., Kasajima, I., Takahashi, H., Kawai-Yamada, M., Uchimiya, H.
  • Journal Title: Metabolomics Volume: 7 Pages: 524-535
  • Peer Reviewed
• [Journal Article] Nicotinate/nicotinamide mononucleotide adenyltransferase-mediated regulation of NAD biosynthesis protects guard cells from reactive oxygen species in ABA-mediated stomatal movement in Arabidopsis (2010)
  • Author(s): Hashida, S-N., Takahashi, H., Takahara, K., Nagano, M., Kawai-Yamada, M., Shoji, K., Goto, F., Yoshihara, T., Uchimiya, H.
  • Journal Title: Journal of Experimental Botany Volume: 61 Pages: 3813-3825
  • Peer Reviewed
• [Journal Article] Metabolome and photochemical analysis of rice plants over-expressing Arabidopsis NAD kinase gene (2010)
  • Author(s): Takahara, K., Kasajima, I., Takahashi, H., Hashida, S., Itami, T, Onodera, H., Toki. S., Yanagisawa, S, Kawai-Yamada, M., Uchimiya, H.
  • Journal Title: Plant Physiology Volume: 152 Pages: 1863-1873
  • Peer Reviewed
• [Journal Article] Targeted metabolomics in an intrusive weed, Rumex obtusifolius L., grown under different environmental conditions reveals alterations of organ related metabolite pathway (2010)
  • Author(s): Miyagi, A., Takahara, K., Takahashi, H., Kawai-Yamada, M., Uchimiya, H.
  • Journal Title: Metabolomics Volume: 6 Pages: 497-510
  • Peer Reviewed
• [Journal Article] Loss of calmodulin binding to bax inhibitor-1 affects pseudomonas-mediated hypersensitive response-associated cell death in Arabidopsis thaliana (2009)
  • Author(s): Kawai-Yamada, M., Hori, Z., Ogawa, T., Ihara-Ohori, Y., Tamura, T., Nagano, M., Ishikawa, T., Uchimiya, H.
  • Journal Title: Journal of Biological Chemistry Volume: 284 Pages: 27998-28003
  • Peer Reviewed
• [Journal Article] Pleiotropic Modulation of Carbon and Nitrogen Metabolism in Arabidopsis Plants Over expressing the NAD kinase2 Gene (2009)
  • Author(s): Takahashi, H., Takahara, K., Hashida, S-N., Hirabayashi, T., Fujimori, T., Kawai-Yamada, M., Yamaya, T., Yanagisawa, S., Uchimiya, H.
  • Journal Title: Plant Physiology Volume: 151 Pages: 100-113
  • Peer Reviewed
• [Journal Article] Pleiotropic modulation of carbon and nitrogen metabolism in Arabidopsis plants overexpressing NAD kinase 2 gene (2009)
  • Author(s): Takahashi,H., Takahara, K., Hashida, S-N., Hirabayashi, T., Fujimori, T., Kawai-Yamada,M., Yamaya,T., Yanagisawa, S., Uchimiya,H.
  • Journal Title: Plant Physiol Volume: 151 Pages: 100-113
  • Peer Reviewed
• [Journal Article] Metabolite Profiling by capillary electrophoresis-mass spectrometry reveals aberrant putrescine accumulation associated with idiopathic symptoms of gentian plants (2009)
  • Author(s): Takahashi, H., Munemura,I., Nakatsuka,T., Nishihara,M., Uchimiya,H.
  • Journal Title: Journal of Horicultural Science Biotechnology Volume: 84 Pages: 312-318
  • Peer Reviewed
• [Journal Article] Loss of calmodulin binding to bax inhibitor-1 affects pseudomonas mediated presensitive respose-cell death in Arabidopsis thaliana (2009)
  • Author(s): Kawai-Yamada,M., Hori,Z., Ogawa,T., Ihara-Ohori,Y., Tamura, T., Nagano, M., Ishikawa, T., Uchimiya,H.
  • Journal Title: J.Biol. Chem. Volume: 284 Pages: 27998-28003
  • Peer Reviewed
• [Journal Article] Estimation of the relative sizes of rate constants for chlorophyll de-excitation processes through comparison of inverse fluorescence intensities (2009)
  • Author(s): Kasajima I., Takahara K., Kawai-Yamada M., Uchimiya H.
  • Journal Title: Plant Cell Phys. Volume: 50 Pages: 1600-1616
  • NAID: 10027343022
  • Peer Reviewed
• [Journal Article] Nakasone A., Kawai-Yamada M., Kiyosue T., Narumi I., Uchimiya H., Oono Y., A gene encoding SMALL CIDIC PROTEIN 2 potentially mediates the response to synthetic auxin, 2,4-dichlorophenozyacetic acid, in Arabidopsis thaliana. (2009)
  • Author(s): Kawai-Yamada, M., Hori,Z., Ogawa, T., Ihara-Ohori, Y., Tamura,T., Nagano,M., Ishikawa, T., Uchimiya,H.
  • Journal Title: J.Plant Phys. Volume: 166 Pages: 1306-1313
  • Peer Reviewed
• [Journal Article] Functional association of cell death suppressor, Arabidopsis Bax Inhibitor-1, with fatty acid 2-hydroxylation through cytochrome b_5 (2009)
  • Author(s): Nagano M., Ihara-Ohori Y., Imai H., Inada N., Fujimoto M., Tsutsumi N., Uchimiya H., Kawai-Yamada M.
  • Journal Title: Plant J. Volume: 58 Pages: 122-134
  • Peer Reviewed
• [Journal Article] CO2 regulator SLAC1 and its homologues are essential for anion homeostasis in plant cells (2008)
  • Author(s): Negi, J., Matsuda, O., Nagasawa, T., Oba, Y., Takahashi, H., Kawai-Yamada, M., Uchimiya, H., Hashimoto, M., Iba, K.
  • Journal Title: Nature Volume: 452 Pages: 483-488
  • Peer Reviewed
• [Journal Article] Difference in metabolite levels between photoatotrophic and photomixotrophic cultures of Synechocystis sp. PCC 6803 examined by capillary electrophoresis electrospray ionization mass spectrometry. (2008)
  • Author(s): Takahashi H, Uchirniya H, Hihara Y
  • Journal Title: J. Exp. Bot. 59 Pages: 3009-3018
  • Peer Reviewed
• [Journal Article] The cell death factor, cell wall elicitor of rice blast fungus (Magnaporthe grisea) causes metabolic alterations including GABA shunt in rice cultured cells. (2008)
  • Author(s): Takahashi, H., Matsumura, H., Kaw ai-Yamada, M., and Uchimiya, H.
  • Journal Title: Plant Sig. Behay. 3 Pages: 1-9
  • Peer Reviewed
• [Journal Article] CO2 regulator SLAC1 and its homologues are essential for anion homeostasis in plant cells. (2008)
  • Author(s): Negi, J., O. Matsuda, T. Nagasawa, Y. Oba, H. Takahashi, M. Kawai-Yamada, H. Uchimiya, M. Hashimoto & K. lba
  • Journal Title: Nature 452 Pages: 483-488
  • Peer Reviewed
• [Presentation] 光阻害耐性のイネ品種間差 (2011)
  • Author(s): 笠島一郎、高原健太郎、川合真紀、内宮博文
  • Organizer: 日本育種学会第117会講演会
  • Place of Presentation: 京都大学吉田キャンパス
  • Year and Date: 2011-03-27
• [Presentation] 光阻害耐性のイネ品種間差 (2010)
  • Author(s): 笠島一郎, 高原健太郎, 川合真紀, 内宮博文
  • Organizer: 日本育種学会第117会講演会
  • Place of Presentation: 京都大学吉田キャンパス
  • Year and Date: 2010-03-27
• [Presentation] ニコチンアミド補酵素代謝を改変した形質転換イネの解析 (2010)
  • Author(s): 高原健太郎、笠島一郎、小野寺治子、土岐精一、川合真紀、内宮博文
  • Organizer: 日本育種学会 第115回講演会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2010-03-27
• [Presentation] パルス変調クロロフィル蛍光測定を利用したイネ品種間差の解析 (2010)
  • Author(s): 笠島一郎、高原健太郎、川合真紀、内宮博文
  • Organizer: 日本育種学会 第115回講演会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2010-03-27
• [Presentation] 細胞死抑制因子Bax inhibitor-1過剰発現イネの代謝適応とストレス耐性 (2010)
  • Author(s): 細胞死抑制因子Bax inhibitor-1過剰発現イネの代謝適応とストレス耐性
  • Organizer: 日本育種学会 第115回講演会
  • Place of Presentation: つくば国際会議場
  • Year and Date: 2010-03-27
• [Presentation] 強害帰化雑草エゾノギシギシの器官別メタボローム解析 (2010)
  • Author(s): 宮城敦子, 高原健太郎, 川合真紀, 内宮博文
  • Organizer: 第5回メタボロームジンポジウム
  • Place of Presentation: 慶応義塾大学先端生命科学研究所(山形)
• [Presentation] Physiological and genetic studies of natural variations in tolerance of photoinhibition in rice (2009)
  • Author(s): Ichiro Kasajima, Kentarou Takahara, Tosio Yamamoto, Masahiro Yano, Maki Kawai-Yamada, Hirofumi Uchimiy
  • Organizer: 6^<th> International Rice Genetics Symposium
  • Place of Presentation: Manila Hotel
  • Year and Date: 2009-11-19
• [Presentation] ニコチンアミド補酵素生合成を改変した形質転換イネの解析 (2009)
  • Author(s): 高原健太郎、笠島一郎、小野寺治子、土岐精一、川合真紀、内宮博文
  • Organizer: 第27回 日本植物細胞分子生物学会
  • Place of Presentation: 日本大学生物資源科学部
  • Year and Date: 2009-07-30
• [Presentation] パルス変調クロロフィル蛍光測定を利用したイネ品種間差の解析 (2009)
  • Author(s): 高橋秀行、中塚貴司、西原昌宏、内宮博文, 代謝解析によるリンドウこぶ症発症原因の探索
  • Organizer: 第27回 日本植物細胞分子生物学会
  • Place of Presentation: 日本大学生物資源科学部
  • Year and Date: 2009-07-30
• [Presentation] イネのクロロフィル蛍光パラメーターとストレス耐性解析 (2009)
  • Author(s): 笠島一郎、高原健太郎、川合真紀、内宮博文
  • Organizer: 第27回 日本植物細胞分子生物学会
  • Place of Presentation: 日本大学生物資源科学部
  • Year and Date: 2009-07-30
• [Presentation] ニコチンアミド補酵素生合成を改変した形質転換イネの解析 (2009)
  • Author(s): 高原健太郎, 笠島一郎, 小野寺治子, 土岐精一, 川合真紀, 内宮博文
  • Organizer: 第27回日本植物細胞分子生物学会(藤沢)大会・シンポジウム
  • Place of Presentation: 日本大学生物資源科学部湘南キャンパス
• [Presentation] パルス変調クロロフィル蛍光測定を利用したイネ品種間差の解析 (2009)
  • Author(s): 笠島一郎, 高原健太郎, 川合真紀, 内宮博文
  • Organizer: 日本育種学会第115回講演会
  • Place of Presentation: つくば国際会議場
• [Presentation] NAD生合成酵素NMNATによる気孔閉鎖運動の制御 (2009)
  • Author(s): 橋田慎之介, 高橋秀行, 高原健太郎, 長野稔, 川合真紀, 内宮博文
  • Organizer: 第50回植物生理学会年会
  • Place of Presentation: 名古屋大学東山キャンパス
• [Presentation] 葉緑体局在型NADリン酸化酵素の高発現がイネの酸化ストレス耐性と一次代謝に及ぼす影響 (2008)
  • Author(s): 高原健太郎, 高橋秀行, 小野寺治子, 土岐精一, 川合真紀, 内宮博文
  • Organizer: 日本育種学会第114回講演会
  • Place of Presentation: 滋賀県立大学
• [Presentation] タデ科ギシギシ属植物の代謝物解析 (2008)
  • Author(s): 宮城敦子, 高橋秀行, 西村芳樹, 高原健太郎, 手塚修文, 川合真紀, 内宮博文
  • Organizer: 日本植物学会72回大会
  • Place of Presentation: 高知大学朝倉キャンパス
• [Presentation] EFFECTS OF OVEREXPRESSION OF AN ARABIDOPSIS NAD KINASE IN THE CHLOROPLAST OF RICE PLANTS ON TOLERANCE TO OXIDATIVE STRESS AND PRIMARY METABOLISM (2008)
  • Author(s): Takahara, K., Takahashi, H., Onodera, H., Toki, S., Kawai-Yamada, M., Uchimiya, H.
  • Organizer: 5th International Conference on Plant Metabolomics
  • Place of Presentation: Pacifico Yokohama-JAPAN
• [Book] Molecular and Genomic Foundation (2010)
  • Author(s): Das, A., Kawai-Yamada, M., Uchimiya, H.
  • Publisher: Programmed Cell Death in Plants, Abiotic Stress Adaptation in Plants : Physiological
• [Book] Programmed Cell Death in Plants, Abiotic Stress Adaptation in Plants : Physiological (2010)
  • Author(s): Das, A., Kawai-Yamada, M., Uchimiya, H.
  • Total Pages: 12
  • Publisher: Molecular and Genomic Foundation (Spreiger)
• [Remarks] 埼玉大学環境科学研究センター
  • URL: http://iest.saitama-u.ac.jp/