| Project/Area Number |
20380014
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | Hokkaido University |
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Principal Investigator |
MASUDA Kiyoshi Hokkaido University, 北方生物圏フィールド科学センター, 教授 (60157203)
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| Co-Investigator(Kenkyū-buntansha) |
SHIOTSUKI Akira 東北大学, 大学院・生命科学研究科, 准教授 (10260449)
YAMAGISHI Masumi 北海道大学, 大学院・農学研究院, 准教授 (40210348)
KOMAI Fuminori 佐賀大学, 農学部, 准教授 (10372765)
SUZUKI Masahiko 北海道大学, 大学院・農学研究院, 教授 (90451418)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2009: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2008: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
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| Keywords | アスパラガス / 超雄性 / 定量PCR / 雄性判別マーカー / 同型接合雄性 / 雌雄性 / 全雄種子 / 雌雄判別マーカー / ホメオティック変異 |
|---|
| Research Abstract |
Homozygous male of asparagus that is called super-male has been used as a pollinator for producing all-male hybrid seeds. However, we do not know a simplified method to discriminate homozygous males (YY) and heterozygous males (XY) in a short period. Then, we grew homozygous and heterozygous male plants from seeds produced by selfing of an andromonoecious line of asparagus and examined the method to identify their genotype. As a result, we found that these genotypes can be identified, based on quantitative analysis of a male-specific diagnostic marker, Asp1-T7.
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