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Analysis of key factor in fruit ripening using DNA array, ripening-impaired mutant and transgenic tomato

Research Project

Project/Area Number 20380022
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionOkayama University

Principal Investigator

KUBO Yasutaka  岡山大学, 大学院・自然科学研究科, 教授 (80167387)

Co-Investigator(Kenkyū-buntansha) YANO Kentaro  明治大学, 農学部, 准教授 (00446543)
Project Period (FY) 2008 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Keywords園芸利用 / エチレン / 果実成熟 / 転写因子 / 変異体 / 形質転換技術 / アレイ解析 / エチレン信号伝達系 / GRAS / 形質転換体 / 形質転換 / 1-MCP / トマト / マクロアレイ / VIGS / 転写制御因子
Research Abstract

We screened ripening key genes using tomato DNA macroarray consisted of 12, 000 clones, ripening-impaired mutants and transgenic technology. Ripening-regulated 428 genes were isolated and 244 genes were regulated under ethylene signaling. As ripening-regulated novel transcription factor or signaling factor, GRAS, SAHH, b-Zip and BTB genes were identified, suggesting their involvement in regulation of fruit ripening at the downstream of ethylene signaling. Transgenic tomatoes with all known LeEILs suppressed, exhibited non-ripening phenotype and indicated that onset of ripening-ethylene is regulated by developmental factors. Based on information in tomato, low-temperature induced ripening in kiwi fruit was analyzed and it was shown to be independent of ethylene signaling.

Report

(6 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report   Self-evaluation Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (34 results)

All 2012 2011 2010 2009 2008

All Journal Article (15 results) (of which Peer Reviewed: 13 results) Presentation (19 results)

  • [Journal Article] Low-temperature-modulated fruit ripening is independent of ethylene in' Sanuki Gold' kiwifruit2012

    • Author(s)
      Mworia, E. G., T. Yoshikawa, N. Salikon, C. Oda, W. O. Asiche, N. Yokotani, D. Abe, K. Ushijima, R. Nakano and Y. Kubo
    • Journal Title

      J. Exp. Bot.

      Volume: 63 Pages: 963-971

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of MA storage and 1-MCP on storability and quality of' Sanuki Gold' kiwifruit harvested at two different maturity stages2011

    • Author(s)
      Mworia, E. G., T. Yoshikawa, N. Salikon, C. Oda, T. Fukuda, K. Suezawa, W. O. Asiche, K. Ushijima, R. Nakano and Y. Kubo
    • Journal Title

      J. Japan. Soc. Hort. Sci.

      Volume: 80 Pages: 372-377

    • NAID

      130004510731

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Large-scale analysis of full-length cDNAs from the tomato(Solanum lycopersicum) cultivar Micro-Tom, a reference system for the Solanaceae genomics.2010

    • Author(s)
      Aoki K., K. Yano, 他
    • Journal Title

      BMC Genomics

      Volume: 11 Pages: 210-210

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparative analysis of differential gene expression inwild-type and 12C5+ ion beam-induced abnormal flower mutant of Solanum villosum by tomato cDNA macroarray.2010

    • Author(s)
      Ojiewo, C., Y. Kubo, Murakami K., and Masuda M.
    • Journal Title

      Int. J. Plant Dev. Biol.

      Volume: 4 Pages: 1-7

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] トマトDNAマクロアレイによるエチレン制御下にある果実成熟関連因子のスクリーニング.2010

    • Author(s)
      閻瑞・上高大典・横谷尚起・牛島幸一郎・中野龍平・久保康隆
    • Journal Title

      園芸学研究別冊2. 9

      Pages: 606-606

    • Related Report
      2010 Self-evaluation Report
  • [Journal Article] トマトDNAマクロアレイによるエチレン制御下にある果実成熟関連因子のスクリーニング2010

    • Author(s)
      閻瑞・上高大典・横谷尚起・牛島幸一郎・中野龍平・久保康隆
    • Journal Title

      園芸学研究 別冊2

      Volume: 9 Pages: 606-606

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Ripening-associated ethylene biosynthesis in tomato fruit is autocatalytically and developmentary regulated.2009

    • Author(s)
      Yokotani N., R. Nakano, S. Imanishi, M. Nagata, A. Inaba and Y. Kubo
    • Journal Title

      J. Exp. Bot.

      Volume: 60 Pages: 3422-3433

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Web databases for Omics data in tomato2009

    • Author(s)
      Suzuki A., Sawabe K., Yano K.
    • Journal Title

      J. Japan. Soc. Hort. Sci.

      Volume: 78 Pages: 23-31

    • NAID

      110007044039

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of ethylene biosynthesis and its regulation during fruit ripening in kiwifruit, Actinidia chinensis' Sanuki Gold'.2009

    • Author(s)
      Mworia E., T. Yoshikawa, N. Yokotani, T. Fukuda, K. Suezawa, K. Ushijima, R. Nakano, Y. Kubo
    • Journal Title

      Postharvest Biol. Technol.

      Volume: 55 Pages: 108-113

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ripening-associated ethylene biosynthesis in tomato fruit is autocatalytically and developmentary regulated.2009

    • Author(s)
      Yokotani N., R.Nakano, S.Imanishi, M.Nagata, A.Inaba, Y.Kubo
    • Journal Title

      J.Exp.Bot. 60

      Pages: 3422-3433

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Web databases for Omics data in tomato.2009

    • Author(s)
      Suzuki A., Sawabe K., Yano K.
    • Journal Title

      J.Japan.Soc.Hort.Sci. 78

      Pages: 23-31

    • NAID

      110007044039

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Characterization of ethylene biosynthesis and its regulation during fruit ripening in kiwifruit, Actinidia chinensis 'Sanuki Gold'.2009

    • Author(s)
      Mworia E., T.Yoshikawa, N.Yokotani, T.Fukuda, K.Suezawa, K.Ushijima, R.Nakano, Y.Kubo
    • Journal Title

      Postharvest Biol.Technol. 55

      Pages: 108-113

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Ripening-associated ethylene biosynthesis in tomato fruit is autocatalytically and developmentary regulated.2009

    • Author(s)
      Yokotani N., R.Nakano, S.Imanishi, M.Nagata, A.Inaba, Y.Kubo
    • Journal Title

      Journal of Expelimantal Botany 60

      Pages: 3422-3433

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of ethylene biosynthesis and its regulation during fruit ripening in kiwifruit, Actinidi a chinensis 'Sanuki Gold'2009

    • Author(s)
      Mworia F., T.Yoshikawa, N.Yokotani, T.Fukuda, K.Suezawa, K.Ushijima, R.Nakano, Y.Kubo
    • Journal Title

      Postharvest Biology and Technology 55

      Pages: 108-113

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Web databases for Oraics data in tomato2009

    • Author(s)
      Suzuki A., Sawabe K., Yano K.
    • Journal Title

      Journal of Japanese Society for Horticultural Scien 78

      Pages: 23-31

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Low-temperature induced fruit ripening is independent of ethylene in fruit2012

    • Author(s)
      Yasutaka Kubo, Eric G.Mworia, Chisato Oda, William O.Asiche, Koichiro Ushijima, Ryohei Nakano
    • Organizer
      第9回植物ホルモン-エチレン国際会議(Ethylene2012)
    • Place of Presentation
      ロトルアコンベンションセンター(ニュージーランド)(招待講演)
    • Year and Date
      2012-03-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] Low-temperature induced fruit ripening is independent of ethylene in' Sanuki Gold' kiwifruit2012

    • Author(s)
      Yasutaka Kubo, Eric G. Mworia, Chisato Oda, William O. Asiche, Koichiro Ushijima, and Ryohei Nakano
    • Organizer
      The 9th International Conference on the Plant Hormone Ethylene
    • Place of Presentation
      Rotorua Convention Center, New Zealnd
    • Related Report
      2011 Final Research Report
  • [Presentation] Regulation of fruit ripening through manipulation of ethylene biosynthesis and signaling2011

    • Author(s)
      Kubo Y
    • Organizer
      AASPP and JKUAT Joint Conference
    • Place of Presentation
      JKUAT, Kenya
    • Year and Date
      2011-11-18
    • Related Report
      2011 Final Research Report
  • [Presentation] Regulation of fruit ripening through manipulation of ethylene biosynthesis and signaling.2011

    • Author(s)
      Kubo Y.
    • Organizer
      AASPP and JKUAT Joint Conference.
    • Place of Presentation
      JKUAT, Kenya.
    • Year and Date
      2011-11-18
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] Transcriptome profiling of ripening associated genes using tomato cDNA macroarray in ethylene and 1-MCP treated fruit2011

    • Author(s)
      Enn Zui, N. Yokotani, K. Ushijima, R. Nakano and Y. Kubo
    • Organizer
      8^<th> Solanaceae and 2^<nd> Cucurbitaceae Genome Joint Conference
    • Place of Presentation
      神戸
    • Related Report
      2011 Final Research Report
  • [Presentation] トマトDNAマクロアレイとVIGS法を利用した果実成熟制御機構の解析2011

    • Author(s)
      閻瑞・上高大典・横谷尚起・牛島幸一郎・中野龍平・久保康隆
    • Organizer
      園芸学会平成23年度秋季大会
    • Place of Presentation
      岡山大学
    • Related Report
      2011 Final Research Report
  • [Presentation] トマトDNAマクロアレイとVIGS法を利用した果実成熟制御機構の解析2011

    • Author(s)
      閻瑞・上高大典・横谷尚起・牛島幸一郎・中野龍平・久保康隆
    • Organizer
      平成23年度園芸学会秋季大会
    • Place of Presentation
      岡山大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] Regulation of fruit ripening through manipulation of ethylene biosynthesis and signaling2010

    • Author(s)
      Kubo Y.
    • Organizer
      AASPP and JKUAT Joint Conference
    • Place of Presentation
      JKUAT, Kenya
    • Year and Date
      2010-11-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Screening of ripening related genes using tomato DNA macroarray, 1-MCP and ripening impaired mutants2010

    • Author(s)
      Kubo Y., N. Yokotani, Y. Rui, D. Kamitaka, K. Ushijima and R. Nakano
    • Organizer
      Gordon Conference for Postharvest Physiology and Technology
    • Place of Presentation
      Tilton School NH USA
    • Year and Date
      2010-06-30
    • Related Report
      2011 Final Research Report
  • [Presentation] Screening of ripening related genes using tomato DNA macroarray, 1-MCP and ripening impaired mutants.2010

    • Author(s)
      Kubo Y., N.Yokotani, Y.Rui, D.Kamitaka, K.Ushijima, R.Nakano
    • Organizer
      Gordon Conference for Postharvest Physiology and Technology.
    • Place of Presentation
      Tilton School NH USA.
    • Year and Date
      2010-06-30
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] Screening of ripening related genes using tomato DNA macroarray, 1-MCP and ripening impaired mutants2010

    • Author(s)
      Kubo Y., N.Yokotani, Y.Rui, D.Kamitaka, K.Ushijima, R.Nakano
    • Organizer
      Gordon Conference for Posyharvest Physiology and Technology
    • Place of Presentation
      Tilton School NH 03276 United States
    • Year and Date
      2010-06-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] Diversity in regulation offruit ripening2010

    • Author(s)
      Kubo Y.
    • Organizer
      JSOL2010
    • Place of Presentation
      東北大学
    • Year and Date
      2010-03-13
    • Related Report
      2011 Final Research Report
  • [Presentation] Diversity in Regulation of Fruit Ripening.2010

    • Author(s)
      Kubo Y.
    • Organizer
      JSOL
    • Place of Presentation
      東北大学
    • Year and Date
      2010-03-13
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] トマトDNAマクロアレイによるエチレン制御下にある果実成熟関連因子のスクリーニング2010

    • Author(s)
      閻瑞・上高大典・横谷尚起・牛島幸一郎・中野龍平・久保康隆
    • Organizer
      園芸学会平成22年度秋季大会
    • Place of Presentation
      大分大学
    • Related Report
      2011 Final Research Report
  • [Presentation] Diversity in Regulation of Fruit Ripening2010

    • Author(s)
      久保康隆
    • Organizer
      JSOL2010
    • Place of Presentation
      東北大学川内荻ホール(宮城県)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Functional screening for the secretomes associated with fruit softening in pear2009

    • Author(s)
      Mwaniki, W. M., R. Nkano, K. Ushijima, K. Aoki, D. Shibata, J. K. C. Rose and Y. Kubo
    • Organizer
      The 8^<th> International Symposium on the Plant Hormone Ethylene
    • Place of Presentation
      Cornell University Ithaca, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] トマトマクロアレイを用いた果実成熟に関連する転写因子の探索2008

    • Author(s)
      久保康隆・上高大典・牛島幸一郎・中野龍平
    • Organizer
      園芸学会平成20年度秋季大会
    • Place of Presentation
      三重大学
    • Related Report
      2011 Final Research Report
  • [Presentation] トマトマクロアレイを用いた果実 成熟に関連する転写因子の探索.2008

    • Author(s)
      久保康隆・上高大典・牛島幸一郎・中野龍平.
    • Organizer
      園芸学会平成20年度秋季大会.
    • Place of Presentation
      三重大学
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] トマトマクロアレイを用いた果実成熟に関連する転写因子の探索2008

    • Author(s)
      久保, 上高, 牛島, 中野
    • Organizer
      園芸学会平成20年度秋季大会研究発表
    • Place of Presentation
      三重大学
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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