Analysis of key factor in fruit ripening using DNA array, ripening-impaired mutant and transgenic tomato
| Project/Area Number |
20380022
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | Okayama University |
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Principal Investigator |
KUBO Yasutaka 岡山大学, 大学院・自然科学研究科, 教授 (80167387)
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| Co-Investigator(Kenkyū-buntansha) |
YANO Kentaro 明治大学, 農学部, 准教授 (00446543)
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| Project Period (FY) |
2008 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2008: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Keywords | 園芸利用 / エチレン / 果実成熟 / 転写因子 / 変異体 / 形質転換技術 / アレイ解析 / エチレン信号伝達系 / GRAS / 形質転換体 / 形質転換 / 1-MCP / トマト / マクロアレイ / VIGS / 転写制御因子 |
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| Research Abstract |
We screened ripening key genes using tomato DNA macroarray consisted of 12, 000 clones, ripening-impaired mutants and transgenic technology. Ripening-regulated 428 genes were isolated and 244 genes were regulated under ethylene signaling. As ripening-regulated novel transcription factor or signaling factor, GRAS, SAHH, b-Zip and BTB genes were identified, suggesting their involvement in regulation of fruit ripening at the downstream of ethylene signaling. Transgenic tomatoes with all known LeEILs suppressed, exhibited non-ripening phenotype and indicated that onset of ripening-ethylene is regulated by developmental factors. Based on information in tomato, low-temperature induced ripening in kiwi fruit was analyzed and it was shown to be independent of ethylene signaling.
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Report
(6 results)
Research Products
(34 results)
